首页 > Jackson > dianova/Ziege IgG anti-Maus IgG (H+L)-HRPO, MinX keine/2 ml/115-035-003

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dianova/Ziege IgG anti-Maus IgG (H+L)-HRPO, MinX keine/2 ml/115-035-003

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ÜbersichtArtikelnummer115-035-003WirtsspeziesZiegeIgG-FormIgG GesamtmolekülSpezies-ReaktivitätMausSpezifitätIgG (H+L)IsotypIgKlonalität (Mono-/Polyklonal)polyklonalAnwendungELISA (Enzyme Linked Immunosorbent Assay), Immuncytochemie, Immunfluoreszenz, Immunhistochemie (Gefrierschnitte), Immunhistochemie (IHC), Immunhistochemie (Paraffingewebe), Western BlotKonjugationHorseradish Peroxidase (HRP)Keine Kreuzreaktivität (MinX) mitkeineVerdünnungELISA 1:5.000 – 1:100.000, Histo-/Zytochemie 1:500 – 1:5.000, Western Blot (ECL): 1:10.000 – 1:200.000, Western Blot (non-ECL): 1:5.000 – 1:100.000Format15 mg/ml BSA (IgG- und Protease-frei), 250 mM NaCl, affinitätsgereinigt durch antigenspezifische Affinitätschromatographie, in 10 mM PBS (pH 7,6), LyophilisatZweckbestimmungnur für ForschungszweckeProduktlinie / ThemaJackson ImmunoResearchHersteller / MarkeJackson ImmunoResearchDatenblätter und Downloads115-035-003 – DatenblattWeitere ProduktinformationenBased on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.ConjugateHorseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

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