Cat#:AER-051-FLyophilizedPowderLyophilizedPowderThisproductisfreezedried.Allwatermoleculeshavebeenremoved.AntigenIncl.AntigenIncl.ThisantibodyisshippedwithitsantigenFREEofcharge!ShippedatRoomTemp.ShippedatRoomTemp.Thislyophilizedproductisshippedatroomtemperature.Pleaseseeitscertificateofanalysisforfurtherstorageinstructions.Type:PolyclonalSource:RabbitReactivity:h,m,rImmunogenPeptideCKQGFLSSNGQNHFK,correspondingtoaminoacidresidues58-72ofhumanEMR1(Accession Q14246).Extracellular,N-terminus.Accession(Uniprot)NumberQ14246GeneID2015PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.HomologyMouse,Rat–11/14aminoacidresiduesidentical.PurityAffinitypurifiedonimmobilizedantigen.FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.LabelFluoresceinisothiocyanate(FITC).StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.Reconstitution50µldoubledistilledwater(DDW).Antibodyconcentrationafterreconstitution1mg/ml.StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°C,protectedfromthelight,forupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000×g5min).ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefortwoweeks.Forlongerperiods,itshouldbestoredat-20°C.Controlantigenreconstitution100µldoubledistilledwater(DDW).Controlantigenstorageafterreconstitution-20°C.PreadsorptionControl1µgpeptideper1µgantibody.StandardqualitycontrolofeachlotWesternblotanalysis(unlabeledantibody,#AER-051),anddirectflowcytometry(labeledantibody).Applications:fcMayalsoworkin:ic,lciDirectflowcytometryDirectflowcytometryofliveintactmouseJ774macrophagecells:___ Cells.___ Cells+rabbitIgGisotypecontrol-FITC.___ Cells+Anti-EMR1(extracellular)-FITCAntibody(#AER-051-F),(2.5µg).DirectflowcytometryofliveintacthumanTHP-1monocyticleukemiacells:___ Cells.___ Cells+rabbitIgGisotypecontrol-FITC.___ Cells+Anti-EMR1(extracellular)-FITCAntibody(#AER-051-F),(2.5µg).ReferencesLegrand,F.etal.(2014)J.AllergyClin.Immunol.133,1439.McKnight,A.J.andGordon,S.(1998)J.Leukoc.Biol.63,271.Araç,D.etal.(2012)EMBOJ.31,1364.ScientificbackgroundHumanepidermalgrowthfactor(EGF)–likemodulecontainingmucin-likehormonereceptor1(EMR1)isasurfacereceptorwithunknownfunctionthatbelongstotheadhesionfamilyoftheGprotein-coupledreceptors(GPCR).ThisreceptorisencodedbytheADGRE1andlikeallGPCRs,containsseventransmembranedomains,anintracellularC-terminusandanextracellularN-terminus.TheNH2domainhassixEGF-likedomains.AdhesionGPCRssharealongN-terminalextracellularsequencescomprisingmultipledomains,separatedfromthetransmembranesegmentsbyaserine/threonine-richdomain,afeaturereminiscentofmucin-like,single-span,integralmembraneglycoproteinswithadhesiveproperties1,2.EMR1proteinisahomologofF4/80,amurineproteinthatiswidelybeingusedasaMarkerofmurinemacrophagepopulations1.HumanEMR1isexclusivelyexpressedonmatureeosinophilsinthebloodandbonemarrow andinnasalpolyps.Thus,EMR1is consideredtobeapotentialtherapeutictargetforthetreatmentofeosinophilicdisorders1,3.Applicationkey:CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-WesternblotSpeciesreactivitykey:H-Human,M-Mouse,R-RatLyophilizedPowderControlAntigenIncludedCertificateofAnalysisMSDSCheckoutmoreofournewproducts.Lastupdate:20/03/2019Anti-EMR1(extracellular)Antibody (#AER-051)isahighlyspecificantibodydirectedagainstanepitopeofthehumanprotein.Theantibodycanbeusedinwesternblotandlivecellimagingapplications.Itrecognizesanextracellularepitopeandisthusidealfordetectingthereceptorinlivingcells.IthasbeendesignedtorecognizeEMR1fromhuman,mouse,andratsamples.Anti-EMR1(extracellular)–FITCAntibody (#AER-051-F)is directlyconjugatedtofluoresceinisothiocyanate(FITC). The antibodycanbeusedinimmunofluorescentapplicationssuchasdirectlivecellflowcytometry.Forresearchpurposesonly,notforhumanuseDirectflowcytometryDirectflowcytometryofliveintactmouseJ774macrophagecells:___ Cells.___ Cells+rabbitIgGisotypecontrol-FITC.___ Cells+Anti-EMR1(extracellular)-FITCAntibody(#AER-051-F),(2.5µg).DirectflowcytometryofliveintacthumanTHP-1monocyticleukemiacells:___ Cells.___ Cells+rabbitIgGisotypecontrol-FITC.___ Cells+Anti-EMR1(extracellular)-FITCAntibody(#AER-051-F),(2.5µg).ReferencesLegrand,F.etal.(2014)J.AllergyClin.Immunol.133,1439.McKnight,A.J.andGordon,S.(1998)J.Leukoc.Biol.63,271.Araç,D.etal.(2012)EMBOJ.31,1364.

       Alomone Labs是您离子通道研究中的伙伴，是离子通道、G蛋白耦联受体和神经信号工具的主要供应商。我们专业生产基础科学研究中所需的一抗、小分子、蛋白质和多肽。Alomone Labs提供的如下生命科学领域研究方案享誉海内外：离子通道研究领域（一系列全面的离子通道研究产品线，包括标记和未标记的抗体及其相关调节因子）、G蛋白耦联受体研究领域（提供了一整套不断增长的GPCR抗体、受体激动剂和拮抗剂产品名单）、 神经信号通路研究（包括一些用于神经营养因子研究的最重要的抗体和生长因子）。
 

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