Cat#:APZ-045LyophilizedPowderLyophilizedPowderThisproductisfreezedried.Allwatermoleculeshavebeenremoved.AntigenIncl.AntigenIncl.ThisantibodyisshippedwithitsantigenFREEofcharge!ShippedatRoomTemp.ShippedatRoomTemp.Thislyophilizedproductisshippedatroomtemperature.Pleaseseeitscertificateofanalysisforfurtherstorageinstructions.Type:PolyclonalSource:RabbitReactivity:h,m,rImmunogenPeptide(C)KAPPLVENEEAEPGR,correspondingtoaminoacidresidues10-24ofhumanGIPC1(AccessionO14908).Intracellular,N-terminus.Accession(Uniprot)NumberO14908GeneID10755PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.HomologyRat,mouse–14/15aminoacidresiduesidentical.PurityAffinitypurifiedonimmobilizedantigen.FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.Reconstitution25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.Antibodyconcentrationafterreconstitution0.8mg/ml.StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefortwoweeks.Forlongerperiods,itshouldbestoredat-20°C.Controlantigenreconstitution100µldoubledistilledwater(DDW).Controlantigenstorageafterreconstitution-20°C.PreadsorptionControl1µgpeptideper1µgantibody.StandardqualitycontrolofeachlotWesternblotanalysis.Applications:ih,wbMayalsoworkin:ic,ipWesternblotWesternblotanalysisofratbrainlysate(lanes1and3)andmousebrainmembranes(lanes2and4):1,2.Anti-GIPC1Antibody(#APZ-045),(1:400).3,4.Anti-GIPC1Antibody,preincubatedwiththecontrolpeptideantigen.WesternblotanalysisofhumanU87-MGglioblastomacelllinelysate:1.Anti-GIPC1 Antibody(#APZ-045),(1:400).2.Anti-GIPC1Antibody,preincubatedwiththecontrolpeptideantigen.ImmunohistochemistryExpressionofGIPC1inmouseandrathippocampusImmunohistochemicalstainingofperfusion-fixedfrozenmouseandratbrainsectionswith Anti-GIPC1Antibody(#APZ-045),(1:200),followedbygoat-anti-rabbit-AlexaFluor-488.A.Inmousebrain,GIPC1staining(green)appearsinneuronsofthepyramidallayer(P)andtheirapicaldendrites(arrows).B.Inratbrainsimilarstainingisobserved.CellnucleiarestainedwithDAPI(blue).ReferencesShang,G.etal.(2017)eLife6,e27322.Katoh,M.(2013)Exp.Mol.Med.45,e26.ScientificbackgroundGIPC1belongstoafamilyofevolutionarilyconservedadaptorproteinsresponsIBLeforthetraffickingofnumeroustransmembraneproteinsviaendocytosis.MembersofGIPC(GAIPinteractingprotein,C-terminus)havethreemaindomainsresponsibleforproteininteractions;aPDZdomainwhichisflankedbyGH1andGH2domains.GH1domainisresponsibleforthedimerizationoftheprotein,whileGH2isimportantfortheinteractionofGIPC1withMYO6,aretrogrademotorproteininvolvedinproteintrafficking.ThePDZdomainofGIPC1isresponsiblefortheproteintointeractwithnumerousproteins;namelyβ1-adrenoceptor,D2dopaminereceptor,GLUT1,TrkAreceptorjusttonameafew.ThezebrafishorthologalsointeractswithNeuropilin-21,2.GIPC1expressionisupregulatedinbreast,ovarianandpancreascancers2.Applicationkey:CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-WesternblotSpeciesreactivitykey:H-Human,M-Mouse,R-RatLyophilizedPowderControlAntigenIncludedClickheretoreceivea25µlfreetrialsample!CertificateofAnalysisMSDSLastupdate:23/10/2018Anti-GIPC1Antibody(#APZ-045)isahighlyspecificantibodydirectedagainstanepitopeofthehumanprotein.Theantibodycanbeusedinwesternblotandimmunohistochemistryapplications.IthasbeendesignedtorecognizeGIPC1fromhuman,rat,andmousesamples.Forresearchpurposesonly,notforhumanuseWesternblotWesternblotanalysisofratbrainlysate(lanes1and3)andmousebrainmembranes(lanes2and4):1,2.Anti-GIPC1Antibody(#APZ-045),(1:400).3,4.Anti-GIPC1Antibody,preincubatedwiththecontrolpeptideantigen.WesternblotanalysisofhumanU87-MGglioblastomacelllinelysate:1.Anti-GIPC1 Antibody(#APZ-045),(1:400).2.Anti-GIPC1Antibody,preincubatedwiththecontrolpeptideantigen.

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