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Alomone/Anti-Adenosine A2B Receptor (extracellular)-FITC Antibody/AAR-003-F/2 x 50 µl
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Cat#:AAR-003-FLyophilizedPowderLyophilizedPowderThisproductisfreezedried.Allwatermoleculeshavebeenremoved.AntigenIncl.AntigenIncl.ThisantibodyisshippedwithitsantigenFREEofcharge!ShippedatRoomTemp.ShippedatRoomTemp.Thislyophilizedproductisshippedatroomtemperature.Pleaseseeitscertificateofanalysisforfurtherstorageinstructions.Type:PolyclonalSource:RabbitReactivity:h,mMayalsoworkin:rImmunogenPeptideKDSATNN(S)TEPWDGTTNESC,correspondingtoaminoacidresidues147-166ofhuman A2BAR(Accession P29275).2nd extracellularloop.Accession(Uniprot)NumberP29275GeneID136PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.HomologyRat -14/19aminoacidresiduesidentical;mouse-13/19aminoacidresiduesidenticalPurityAffinitypurifiedonimmobilizedantigen.FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.LabelFluoresceinisothiocyanate(FITC).StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.Reconstitution50µldoubledistilledwater(DDW).Antibodyconcentrationafterreconstitution1mg/ml.StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°C,protectedfromthelight,forupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000×g5min).ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.Controlantigenreconstitution100µldoubledistilledwater(DDW).Controlantigenstorageafterreconstitution-20°C.PreadsorptionControl1µgpeptideper1µgantibody.StandardqualitycontrolofeachlotWesternblotanalysis(unlabeledantibody,#AAR-003),anddirectflowcytometry(labeledantibody).Applications:fcMayalsoworkin:ic,lciDirectflowcytometryDirectflowcytometryofliveintacthumanTHP-1monocyticleukemiacells:___Cellsalone.___Cells+RabbitIgGisotypecontrol-FITC.___Cells+Adenosine A2B Receptor (extracellular)-FITCAntibody(AAR-003-F),5µg.DirectflowcytometryofliveintactmouseJ774macrophagecells:___Cellsalone.___Cells+RabbitIgGisotypecontrol-FITC.___Cells+Adenosine A2B Receptor (extracellular)-FITCAntibody(AAR-003-F),5µg.ReferencesPeeters,M.C.etal.(2011)FASEBJ.25,632.Sun,Y.andHuang,P.(2016)Front.Chem.4,37.ScientificbackgroundExtracellularadenosineisaubiquitoussignalingmoleculethatmodulatesawidearrayofBIOLOGicalprocesses.Therearecurrentlyfourknownsub-typesofadenosinereceptors:A1,A2A,A2B andA3. TheadenosineA2B receptorisatypicalclassA,Gprotein-coupledreceptorwithseventransmembranesegments,anextracellularN-terminusandanintracellularC-terminus.AdenosineA2BRcausesanincreaseinintracellularcAMPlevelsbycouplingmainlytoGs proteins,resultingintheactivationofadenylylcyclase1 andsubsequentactivationofPKAandothercAMPeffectorssuchasEpac.Inaddition,theA2BRcouplestoGq andactivatesthePLCpathwaythatmediatesseveralcrucialfunctionsofA2BR.A2BRalsocouplestotheMAPKandarachidonicacidsignalingpathwaysandregulatesmembraneionchannelsprobablythroughG-proteinβγsubunits. MultiplemoleculesthatbindtoA2BRhavebeenidentifiedinanefforttounderstandthereceptor’sfunctionandregulation.NFκB1/p105isamemberoftheNFκBfamilyofproteinsthatperformregulatoryfunctionsindiversebiologicalprocessessuchasinflammationandcellsurvivalanddifferentiation,aswellasinvariousdiseases.Interestingly,AlthoughA2BRactivationbyadenosineproducespro-inflammatoryeffectsthereceptoralsobindstospecificsitesonp105,preventsthepoly-ubiquitinationanddegradationofthep105proteinandinhibitsNFκBactivationthusreducinginflammation. A2BRishighlyexpressedinvarioustypesoftumorcellsortissuesandpromotestumor-cellproliferation.A2BRwasfoundtobeoverexpressedincolorectalcarcinomacellsandtissues,andinhibitionofA2BRblockstheproliferationofcoloncancercells.A2BRhasbeenimplicatedinthepathophysiologyofvasculardiseases,renaldiseaseanddiabetes2.Applicationkey:CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-WesternblotSpeciesreactivitykey:H-Human,M-Mouse,R-RatLyophilizedPowderControlAntigenIncludedCertificateofAnalysisMSDSLastupdate:24/03/2019Anti-AdenosineA2B Receptor(extracellular)Antibody(#AAR-003)isahighlyspecificantibodydirectedagainstanextracellularepitopeofthehumanprotein.Theantibodycanbeusedinwesternblot,immunohistochemistry,livecellimaging,andindirectflowcytometryapplications.IthasbeendesignedtorecognizeA2BARfromhuman,rat,andmousesamples.Anti-Adenosine A2B Receptor(extracellular)-FITCAntibody(#AAR-003-F)isdirectlyconjugatedtofluoresceinisothiocyanate(FITC).Thislabeledantibodycanbeusedinimmunofluorescentapplicationssuchasdirectflowcytometryusinglivecells.Forresearchpurposesonly,notforhumanuseDirectflowcytometryDirectflowcytometryofliveintacthumanTHP-1monocyticleukemiacells:___Cellsalone.___Cells+RabbitIgGisotypecontrol-FITC.___Cells+Adenosine A2B Receptor (extracellular)-FITCAntibody(AAR-003-F),5µg.DirectflowcytometryofliveintactmouseJ774macrophagecells:___Cellsalone.___Cells+RabbitIgGisotypecontrol-FITC.___Cells+Adenosine A2B Receptor (extracellular)-FITCAntibody(AAR-003-F),5µg.ReferencesPeeters,M.C.etal.(2011)FASEBJ.25,632.Sun,Y.andHuang,P.(2016)Front.Chem.4,37.

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