Cat#:AAR-016LyophilizedPowderLyophilizedPowderThisproductisfreezedried.Allwatermoleculeshavebeenremoved.AntigenIncl.AntigenIncl.ThisantibodyisshippedwithitsantigenFREEofcharge!ShippedatRoomTemp.ShippedatRoomTemp.Thislyophilizedproductisshippedatroomtemperature.Pleaseseeitscertificateofanalysisforfurtherstorageinstructions.Type:PolyclonalSource:RabbitReactivity:m,rMayalsoworkin:hImmunogenPeptide(C)NGSRAPDHDVTQERDE,correspondingtoaminoacidresidues15-30ofmouseADRB2 (AccessionP18762).Extracellular,N-terminus.Accession(Uniprot)NumberP18762GeneID11555PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.HomologyHuman-15/16aminoacidresiduesidentical;rat-14/16aminoacidresiduesidentical.PurityAffinitypurifiedonimmobilizedantigen.FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.Reconstitution25µl,50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.Antibodyconcentrationafterreconstitution0.7mg/ml.StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.Controlantigenreconstitution100µldoubledistilledwater(DDW).Controlantigenstorageafterreconstitution-20°C.PreadsorptionControl1μgpeptideper1μgantibody.StandardqualitycontrolofeachlotWesternblotanalysis.Applications:ih,wbMayalsoworkin:ic,ifc,ip,lciWesternblotWesternblotanalysisofratheart(lanes1and3)andlung(lanes2and4)lysates:1,3.Anti-β2-AdrenergicReceptor(extracellular) Antibody(#AAR-016),(1:200).2,4.Anti-β2-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.ImmunohistochemistryExpressionof β2-AdrenoceptorinratlungImmunohistochemicalstainingofparaffin-embeddedsectionofratlungusingAnti-β2-AdrenergicReceptor(extracellular) Antibody(#AAR-016),(1:100).β2-Adrenoceptorisexpressedintherespiratoryepithelium.Hematoxilinisusedasthecounterstain.ReferencesIUPHARRECEPTORDATABASE|ADRENOCEPTORSPiascik,M.T.andPerez,D.M.(2001)J.Pharmacol.Exp.Ther.298,403.Pullar,C.E.etal.(2006)FASEBJ.20,76.Oostendorp,J.etal(2005)Am.J.Respir.Crit.CareMed.172,322.Giembycz,M.A.andNewton,R.(2006)Eur.Respir.J.27,1286.ScientificbackgroundAdrenergicreceptors(alsocalledadrenoceptors)arethereceptorsforthecatecholaminesadrenalineandnoradrenaline(calledepinephrineandnorepinephrineintheUnitedStates).Adrenalineandnoradrenalineplayimportantrolesinthecontrolofbloodpressure,myocardialcontractilerateandforce,airwayreactivity,andavarietyofmetabolicandcentralnervoussystemfunctions. AdrenergicreceptorsaremembersoftheG-proteincoupledreceptor(GPCR)superfamilyofmembraneproteins.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularaminoterminus,andacytoplasmiccarboxylterminus.Adrenoceptorsaredividedintothreetypes:α1,α2 andβ-adrenoceptors.Eachtypeisfurtherdividedintoatleastthreesubtypes:α1A,α1B,α1D,α2A,α2B,α2C,β1,β2,β3.1,2 Adrenoceptors areexpressedinnearlyallperipheraltissuesandinthecentralnervoussystem.1,2 β2-Adrenoceptorsaremainlypostsynapticreceptors.Theyareexpressedonanumberoftissuesincludingbloodvessels,bronchi,gastrointestinaltract(GIT),skeletalmuscle,liverandmastcell.3 Functionalβ2-adrenoceptorswerealsofoundonlymphocytes,macrophagesandneutrophils.4,5 Theyarealsotheonlyβ-adrenoceptorsubtypeexpressedinkeratinocytes,fibroblastsandmelanocytes.3 Activationofβ2-adrenoceptorsresultsinvasodilation,relaxationoftheGIT,inhibitionofhistaminereleasefrommastcellsandbronchodilation.SelectiveagoNISTsofβ2-adrenoceptorsareusedtotreatasthmaandotherrelatedbronchospasticconditionssuchasChronicobstructivepulmonarydisease(COPD).5 Applicationkey:CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-WesternblotSpeciesreactivitykey:H-Human,M-Mouse,R-RatLyophilizedPowderControlAntigenIncludedClickheretoreceivea25µlfreetrialsample!CertificateofAnalysisMSDSLastupdate:01/01/2019Anti-β2-AdrenergicReceptor(extracellular)Antibody(#AAR-016)isahighlyspecificantibodydirectedagainstanextracellularepitopeofthemouseβ2-adrenoceptor.Theantibodycanbeusedinwesternblotandimmunohistochemistryapplications.Ithasbeendesignedtorecognizeβ2ARfrommouse,rat,andhumansamples.Forresearchpurposesonly,notforhumanuseWesternblotWesternblotanalysisofratheart(lanes1and3)andlung(lanes2and4)lysates:1,3.Anti-β2-AdrenergicReceptor(extracellular) Antibody(#AAR-016),(1:200).2,4.Anti-β2-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.

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