Cat#:AAR-018-AGLyophilizedPowderLyophilizedPowderThisproductisfreezedried.Allwatermoleculeshavebeenremoved.AntigenIncl.AntigenIncl.ThisantibodyisshippedwithitsantigenFREEofcharge!ShippedatRoomTemp.ShippedatRoomTemp.Thislyophilizedproductisshippedatroomtemperature.Pleaseseeitscertificateofanalysisforfurtherstorageinstructions.Type:PolyclonalSource:RabbitReactivity:h,rImmunogenPeptide(C)KNANFTGPNQTSSNS,correspondingtoaminoacidresidues21-35ofhumanα1B-adrenoceptor(Accession P35368).Extracellular,N-terminus.Accession(Uniprot)NumberP35368GeneID147PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.HomologyMouse-14/15aminoacidresiduesidentical;rat-13/15aminoacidresiduesidentical.PurityAffinitypurifiedonimmobilizedantigen.FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.LabelATTO-488.Maximumabsorption501nm;maximumfluorescence523nm.Thefluorescenceisexcitedmostefficientlyinthe480–515nmrange.Thislabelisanalogoustothewellknowndyefluoresceinisothiocyanate(FITC)andcanbeusedwithfilterstypicallyusedtodetectFITC.StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.Reconstitution50µldoubledistilledwater(DDW).Antibodyconcentrationafterreconstitution1mg/ml.StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°C,protectedfromthelight,forupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000×g5min).ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.Controlantigenreconstitution100µldoubledistilledwater(DDW).Controlantigenstorageafterreconstitution-20°C.PreadsorptionControl1μgpeptideper1μgantibody.StandardqualitycontrolofeachlotWesternblotanalysis(unlabeledantibody,#AAR-018),andlivecellimaging(labeledantibody).Applications:fc,ic,lciMayalsoworkin:ihDirectflowcytometryDirectflowcytometryanalysisinratGH3pituitaryadenomacells:___Cells.___Cells+ Anti-α1B-AdrenergicReceptor(extracellular)-ATTO-488 Antibody(#AAR-018-AG),(10µg/5x105 cells).Livecellimaging/ImmunocytochemistryExpressionofα1B-AdrenoceptorinratGH3cellsImmunocytochemicalstainingofGH3cellswithAnti-α1B-AdrenergicReceptor(extracellular)-ATTO-488Antibody (#AAR-018-AG)(green).Nuclearfluorescencestainingofcellsusingthemembrane-permeableDNAdyeHoechst33342(blue).ReferencesIUPHARRECEPTORDATABASE|ADRENOCEPTORSPiascik,M.T.andPerez,D.M.(2001)J.Pharmacol.Exp.Ther.298,403.Perez,D.M.etal.(1993)Mol.Pharmacol.44,784.Minneman,K.P.(1988)Pharmacol.Rev.40,87.Schwinn,D.A.etal.(2004)MayoClin.Proc.79,1423.ScientificbackgroundAdrenergicreceptors(alsocalledadrenoceptors)arethereceptorsforthecatecholaminesadrenalineandnoradrenaline(calledepinephrineandnorepinephrineintheUnitedStates).Adrenalineandnoradrenalineplayimportantrolesinthecontrolofbloodpressure,myocardialcontractilerateandforce,airwayreactivity,andavarietyofmetabolicandcentralnervoussystemfunctions. Adrenergicreceptorsaremembersofthe G-proteincoupledreceptor (GPCR)superfamilyofmembraneproteins.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularaminoterminus,andacytoplasmiccarboxylterminus.Adrenoceptorsaredividedintothreetypes:α1,α2 andβadrenoceptors.Eachtypeisfurtherdividedintoatleastthreesubtypes:α1A,α1B,α1D,α2A,α2B,α2C,β1,β2,β3.1,2 Theyareexpressedinnearlyallperipheraltissuesandinthecentralnervoussystem.1,2 Allα1-adrenoceptors(α1-ARs)activatephospholipasesCandA2.3 Inadditiontomobilizingintracellularcalcium,theα1-ARshavealsobeenshowntoactivatecalciuminfluxviavoltage-dependentand-independentcalciumchannels.4 α1B-Adrenoceptorpopulationsaregreatestinthespleen,kidney,cerebellum,andfetalbrain.5α1B-Adrenoceptorcausescontractionofsmoothmusclecellsandtherebycontrolsvasculartone,bloodpressure,andacceleratesthedevelopmentofatherosclerosis.5Applicationkey:CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-WesternblotSpeciesreactivitykey:H-Human,M-Mouse,R-RatLyophilizedPowderControlAntigenIncludedCertificateofAnalysisMSDSLastupdate:24/03/2019Anti-α1B–AdrenergicReceptor(extracellular)Antibody(#AAR-018)isahighlyspecificantibodydirectedagainstanextracellularepitopeofthehumanα1B-adrenoceptor.Theantibodycanbeusedinwesternblot,immunohistochemistry,livecellimagingandindirectflowcytometryapplications.Ithasbeendesignedtorecognizeα1B-adrenoceptorfromhuman,ratandmousesamples.Anti-α1B-AdrenergicReceptor(extracellular)-ATTO-488Antibody(#AAR-018-AG)isdirectlylabeledwithanATTO-488fluorescentdye.ATTOdyesarecharacterizedbystrongabsorption(highextinctioncoefficient),highfluorescencequantumyield,andhighphoto-stABIlity.TheATTO-488labelisanalogoustothewellknowndyefluoresceinisothiocyanate(FITC)andcanbeusedwithfilterstypicallyusedtodetectFITC.Anti-α1B-AdrenergicReceptor(extracellular)-ATTO-488Antibody hasbeentestedindirectflowcytometryapplicationsandisspeciallysuitedtoexperimentsrequiringsimultaneouslabelingofdifferentMarkers.Forresearchpurposesonly,notforhumanuseDirectflowcytometryDirectflowcytometryanalysisinratGH3pituitaryadenomacells:___Cells.___Cells+ Anti-α1B-AdrenergicReceptor(extracellular)-ATTO-488 Antibody(#AAR-018-AG),(10µg/5x105 cells).Livecellimaging/ImmunocytochemistryExpressionofα1B-AdrenoceptorinratGH3cellsImmunocytochemicalstainingofGH3cellswithAnti-α1B-AdrenergicReceptor(extracellular)-ATTO-488Antibody (#AAR-018-AG)(green).Nuclearfluorescencestainingofcellsusingthemembrane-permeableDNAdyeHoechst33342(blue).ReferencesIUPHARRECEPTORDATABASE|ADRENOCEPTORSPiascik,M.T.andPerez,D.M.(2001)J.Pharmacol.Exp.Ther.298,403.Perez,D.M.etal.(1993)Mol.Pharmacol.44,784.Minneman,K.P.(1988)Pharmacol.Rev.40,87.Schwinn,D.A.etal.(2004)MayoClin.Proc.79,1423.

       Alomone Labs是您离子通道研究中的伙伴，是离子通道、G蛋白耦联受体和神经信号工具的主要供应商。我们专业生产基础科学研究中所需的一抗、小分子、蛋白质和多肽。Alomone Labs提供的如下生命科学领域研究方案享誉海内外：离子通道研究领域（一系列全面的离子通道研究产品线，包括标记和未标记的抗体及其相关调节因子）、G蛋白耦联受体研究领域（提供了一整套不断增长的GPCR抗体、受体激动剂和拮抗剂产品名单）、 神经信号通路研究（包括一些用于神经营养因子研究的最重要的抗体和生长因子）。
 

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