Cat#:AAR-023LyophilizedPowderLyophilizedPowderThisproductisfreezedried.Allwatermoleculeshavebeenremoved.AntigenIncl.AntigenIncl.ThisantibodyisshippedwithitsantigenFREEofcharge!ShippedatRoomTemp.ShippedatRoomTemp.Thislyophilizedproductisshippedatroomtemperature.Pleaseseeitscertificateofanalysisforfurtherstorageinstructions.Type:PolyclonalSource:RabbitReactivity:m,rMayalsoworkin:hImmunogenPeptide(C)RAESDEARR(S)YNDP,correspondingtoaminoacidresidues200-214ofratADRB1 (Accession P18090).2nd extracellularloop.Accession(Uniprot)NumberP18090GeneID64508PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.HomologyRat,Mouse,human-14/15aminoacidresiduesidentical.PurityAffinitypurifiedonimmobilizedantigen.FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.Reconstitution25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.Antibodyconcentrationafterreconstitution0.85mg/ml.StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.Controlantigenreconstitution100µldoubledistilledwater(DDW).Controlantigenstorageafterreconstitution-20°C.PreadsorptionControl1µgpeptideper1µgantibody.StandardqualitycontrolofeachlotWesternblotanalysis.Applications:ih,wbMayalsoworkin:ic,ifc,ip,lciWesternblotWesternblotanalysisofratbrain(lanes1and4),mousebrain(lanes2and5)andratlung(lanes3and6)membranes:1-3. Anti-β1-AdrenergicReceptor(extracellular) Antibody(#AAR-023),(1:200).4-6.Anti-β1-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.ImmunohistochemistryExpressionofβ1-AdrenoceptorinratandmousebrainImmunohistochemicalstainingofratdorsalraphenucleusandmousecerebellumusing Anti-β1-AdrenergicReceptor(extracellular) Antibody(#AAR-023),(1:400). A. β1-Adrenoceptorstaining(red) inratdorsalraphenucleusappearsinneurons(horizontalarrows). C. β1-Adrenoceptor inmousecerebellumstaining(red)isdetectedingranulecells(horizontalarrows)andinthePurkinjecelllayer(verticalarrow). B,D.NuclearstainingusingDAPIasthecounterstain(blue).ReferencesGilbert,C.E.etal.(2014)Int.J.Mol.Sci.15,2929.Woo,A.Y.etal.(2012)ActaPharmacol.Sin.33,335.Vicco,M.H.etal.(2014)Can.J.Cardio.30,332.Wallukat,G.etal.(2002)Herz27,683.Kamal,F.A.etal.(2014)Circ.Res.114,742.Scientificbackgroundβ-Adrenergicreceptors(βAR)belongtothesuperfamilyof G-proteincoupledreceptors (GPCRs).βARstimulationbythesympatheticnervoussystemorcirculatingcatecholaminesisinvolvedinperipheralbloodcirculation,metabolicregulation,musclecontraction,andcentralneuralactivities.Intheheart,acuteβARstimulationservesasthemostpowerfulmeanstoregulatecardiacoutputinresponsetoafight-or-flightsituation.TherearethreeβARsubtypes,β1AR, β2AR and β3AR,incardiacmyocytes1.Thereceptorsconsistofsevenmembrane-spanningdomains,threeintra-andthreeextracellularloops,anextracellularN-terminaldomain,andanintracellularC-terminaltail2.β1ARisthepredominantreceptorsubtypeexpressedinhumancardiomyocytes.β1ARisalsoexpressedinthekidney3.IthasbeenimplicatedinADIpogenesisandmemoryformation4.β1ARhasbeenwidelyconnectedwithheartdisease.Boththedownregulationandoverstimulationofβ1ARhavebeenconnectedtomyocyteapoptosis.Inaddition,chronicheartfailureischaracterizedbyabnormalitiesinβ1ARsignaltransduction.ItwasdemonstratedthatheartfailureisalsoinducIBLeinanimalmodelsbyoverexpressionofcomponentsoftheβ1ARsignaltransductioncascade.Transgenicmiceoverexpressingβ1ARdevelop,withaging,heartfailuresimilartothatobservedindilatedcardiomyopathyinhumans5.Applicationkey:CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-WesternblotSpeciesreactivitykey:H-Human,M-Mouse,R-RatLyophilizedPowderControlAntigenIncludedClickheretoreceivea25µlfreetrialsample!CertificateofAnalysisMSDSLastupdate:26/12/2018Anti-β1-AdrenergicReceptor(extracellular)Antibody(#AAR-023)isahighlyspecificantibodydirectedagainstanextracellularepitopetheratβ1-adrenoceptor.Theantibodycanbeusedinwesternblotandimmunohistochemistryapplications.Theantibodyrecognizesanextracellularepitopeandisthusidealfordetectingthereceptorinlivingcells.Ithasbeendesignedtorecognizeβ1ARfrommouse,ratandhumansamples.Forresearchpurposesonly,notforhumanuseWesternblotWesternblotanalysisofratbrain(lanes1and4),mousebrain(lanes2and5)andratlung(lanes3and6)membranes:1-3. Anti-β1-AdrenergicReceptor(extracellular) Antibody(#AAR-023),(1:200).4-6.Anti-β1-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.

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