Cat#:AAR-022LyophilizedPowderLyophilizedPowderThisproductisfreezedried.Allwatermoleculeshavebeenremoved.AntigenIncl.AntigenIncl.ThisantibodyisshippedwithitsantigenFREEofcharge!ShippedatRoomTemp.ShippedatRoomTemp.Thislyophilizedproductisshippedatroomtemperature.Pleaseseeitscertificateofanalysisforfurtherstorageinstructions.Type:PolyclonalSource:RabbitReactivity:rMayalsoworkin:h,mImmunogenPeptide(C)RRPDGAAYPQ*SGLNDE,correspondingtoaminoacidresidues192-207ofratα2C-adrenoceptorwithreplacementofcysteine202(C202)withserine(*S)(Accession P22086).2nd extracellularloop.Accession(Uniprot)NumberP22086GeneID24175PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.HomologyRat,mouse-15/16aminoacidresiduesidentical;human-14/16aminoacidresiduesidentical.PurityAffinitypurifiedonimmobilizedantigen.FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.Reconstitution50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.Antibodyconcentrationafterreconstitution0.8mg/ml.StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.Controlantigenreconstitution100µldoubledistilledwater(DDW).Controlantigenstorageafterreconstitution-20°C.PreadsorptionControl1μgpeptideper1μgantibody.StandardqualitycontrolofeachlotWesternblotanalysis.Applications:ih,wbMayalsoworkin:ic,ifc,ip,lciWesternblotWesternblotanalysisofratlung(lanes1and4),kidney(lanes2and5)andbrain(lanes3and6)membranes:1-3. Anti-α2C-AdrenergicReceptor(extracellular)Antibody(#AAR-022),(1:200).4-6.Anti-α2C-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.ImmunohistochemistryExpressionofα2C-AdrenoreceptorinratkidneyImmunohistochemicalstainingofratkidneyparaffinembeddedsectionsusing Anti-α2C-AdrenergicReceptor(extracellular) Antibody(#AAR-022),(1:100).α2C-Adrenoreceptorisexpressedinbothproximal(PT)anddistal(DT)renaltubules andinthecollectingducts(CD).Hematoxilinisusedasthecounterstain.ReferencesIUPHARRECEPTORDATABASE|ADRENOCEPTORS.Piascik,M.T.andPerez,D.M.(2001)J.Pharmacol.Exp.Ther.298,403.Murphy,T.J.andBylund,D.B.(1988)J.Pharmacol.Exp.Ther.244,571.Blaxall,H.S.etal.(1991)J.Pharmacol.Exp.Ther.259,323.BjorklindM.etal.(1999)Neuroscience88,1187.TanilaH.etal.(1999)Eur.J.Neurosci.11,599.ScientificbackgroundAdrenergicreceptors(alsocalledadrenoceptors)arethereceptorsforthecatecholaminesadrenalineandnoradrenaline(calledepinephrineandnorepinephrineintheUnitedStates).Adrenalineandnoradrenalineplayimportantrolesinthecontrolofbloodpressure,myocardialcontractilerateandforce,airwayreactivity,andavarietyofmetabolicandcentralnervoussystemfunctions.AdrenergicreceptorsaremembersoftheG-proteincoupledreceptor(GPCR)superfamilyofmembraneproteins.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularN-terminus,andacytoplasmicC-terminus.Adrenoceptorsaredividedintothreetypes:α1,α2 andβ-adrenoceptors.Eachtypeisfurtherdividedintoatleastthreesubtypes:α1A,α1B,α1D,α2A,α2B,α2C,β1,β2,β31,2.Adrenoceptors areexpressedinnearlyallperipheraltissuesandinthecentralnervoussystem1,2.Theα2C-adrenoceptorsubtypewasidentifiedbybindingstudiesintheopossumkidneyandincelllinesfromthisorgan(OKcells)3,4.Surprisingly,theα2C-adrenoceptorsubtypeseemsnottoplayamajorroleincardiovascularregulationortheotherclassicaleffectsofα2-adrenergicreceptors5.Someresearchershavesuggestedthattheα2C-adrenoceptorsubtypemayplayaroleinmodulatingmotorbehaviourandperhapsinmemoryprocesses6,7.Applicationkey:CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-WesternblotSpeciesreactivitykey:H-Human,M-Mouse,R-RatLyophilizedPowderControlAntigenIncludedClickheretoreceivea50µlfreetrialsample!CertificateofAnalysisMSDSLastupdate:01/11/2018AlomoneLabsispleasedtoofferahighlyspecificantibodydirectedagainstanextracellularepitopeoftheratα2C-adrenoceptor.Anti-α2C-AdrenergicReceptor(extracellular)Antibody(#AAR-022)canbeusedforwesternblotandimmunohistochemistryapplications.Ithasbeendesignedtorecognizeα2C-adrenoceptorfromrat,mouseandhumansamples.Forresearchpurposesonly,notforhumanuseWesternblotWesternblotanalysisofratlung(lanes1and4),kidney(lanes2and5)andbrain(lanes3and6)membranes:1-3. Anti-α2C-AdrenergicReceptor(extracellular)Antibody(#AAR-022),(1:200).4-6.Anti-α2C-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.

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