Cat#:AAR-018LyophilizedPowderLyophilizedPowderThisproductisfreezedried.Allwatermoleculeshavebeenremoved.AntigenIncl.AntigenIncl.ThisantibodyisshippedwithitsantigenFREEofcharge!ShippedatRoomTemp.ShippedatRoomTemp.Thislyophilizedproductisshippedatroomtemperature.Pleaseseeitscertificateofanalysisforfurtherstorageinstructions.Type:PolyclonalSource:RabbitReactivity:h,rMayalsoworkin:mImmunogenPeptide(C)KNANFTGPNQTSSNS,correspondingtoaminoacidresidues21-35ofhumanα1B-adrenoceptor(AccessionP35368).Extracellular,N-terminus.Accession(Uniprot)NumberP35368GeneID147PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.HomologyMouse-14/15aminoacidresiduesidentical;rat-13/15aminoacidresiduesidentical.PurityAffinitypurifiedonimmobilizedantigen.FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.Reconstitution50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.Antibodyconcentrationafterreconstitution0.8mg/ml.StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.Controlantigenreconstitution100µldoubledistilledwater(DDW).Controlantigenstorageafterreconstitution-20°C.PreadsorptionControl1μgpeptideper1μgantibody.StandardqualitycontrolofeachlotWesternblotanalysis.Applications:ic,ifc,ih,lci,wbMayalsoworkin:ipWesternblotWesternblotanalysisofα1B-adrenoreceptorinlysatesofratbrain(lanes1and3),ratkidney(lanes2and4)andGH3cellline(lanes5and6):1,2,5.Anti-α1B-AdrenergicReceptor(extracellular)Antibody(#AAR-018),(1:200).3,4,6.Anti-α1B-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.ImmunohistochemistryExpressionofα1B-AdrenoreceptorinratcerebellumImmunohistochemicalstainingoffrozensectionofratcerebellumusingAnti-α1B-AdrenergicReceptor(extracellular)Antibody (#AAR-018),(1:100).α1B-Adrenoceptor(red)isdetectedinPurkinjecells.Hoechst33342wasusedasthecounterstain.IndirectflowcytometryIndirectflowcytometryanalysisofGH3livingcells:___Unstainedcells.___Cells+Anti-α1B-AdrenergicReceptor(extracellular)Antibody(#AAR-018),(10µg/5x105cells).Thecontrolantigenisnotsuitableforthisapplication.Livecellimaging/ImmunocytochemistryExpressionofα1B-AdrenoceptorinGH3cellsCellsurfacedetectionof α1B-AdrenoceptorinlivingGH3cellswithAnti-α1B-AdrenergicReceptor (extracellular)Antibody (#AAR-018),followedbygoatanti-rabbit-AlexaFluor-488secondaryantibody(green).NuclearstainingofcellsusingtheDNAdyeHoechst33342(blue).ReferencesIUPHARRECEPTORDATABASE|ADRENOCEPTORSPiascik,M.T.andPerez,D.M.(2001)J.Pharmacol.Exp.Ther.298,403.Perez,D.M.etal.(1993)Mol.Pharmacol.44,784.Minneman,K.P.(1988)Pharmacol.Rev.40,87.Schwinn,D.A.etal.(2004)MayoClin.Proc.79,1423.ScientificbackgroundAdrenergicreceptors(alsocalledadrenoceptors)arethereceptorsforthecatecholaminesadrenalineandnoradrenaline(calledepinephrineandnorepinephrineintheUnitedStates).Adrenalineandnoradrenalineplayimportantrolesinthecontrolofbloodpressure,myocardialcontractilerateandforce,airwayreactivity,andavarietyofmetabolicandcentralnervoussystemfunctions. Adrenergicreceptorsaremembersofthe G-proteincoupledreceptor (GPCR)superfamilyofmembraneproteins.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularaminoterminus,andacytoplasmiccarboxylterminus.Adrenoceptorsaredividedintothreetypes:α1,α2 andβadrenoceptors.Eachtypeisfurtherdividedintoatleastthreesubtypes:α1A,α1B,α1D,α2A,α2B,α2C,β1,β2,β3.1,2 Theyareexpressedinnearlyallperipheraltissuesandinthecentralnervoussystem.1,2 Allα1-adrenoceptors(α1-ARs)activatephospholipasesCandA2.3 Inadditiontomobilizingintracellularcalcium,theα1-ARshavealsobeenshowntoactivatecalciuminfluxviavoltage-dependentand-independentcalciumchannels.4 α1B-Adrenergicreceptorpopulationsaregreatestinthespleen,kidney,cerebellum,andfetalbrain.5α1B-Adrenoceptorcausescontractionofsmoothmusclecellsandtherebycontrolsvasculartone,bloodpressure,andacceleratesthedevelopmentofatherosclerosis.5 Applicationkey:CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-WesternblotSpeciesreactivitykey:H-Human,M-Mouse,R-RatLyophilizedPowderControlAntigenIncludedClickheretoreceivea50µlfreetrialsample!CertificateofAnalysisMSDSLastupdate:19/03/2019Anti-α1B–AdrenergicReceptor(extracellular)Antibody(#AAR-018)isahighlyspecificantibodydirectedagainstanextracellularepitopeofthehumanα1B-adrenoceptor.Theantibodycanbeusedinwesternblot,immunohistochemistry,livecellimagingandindirectflowcytometryapplications.Ithasbeendesignedtorecognizeα1B-adrenoceptorfromhuman,ratandmousesamples.Forresearchpurposesonly,notforhumanuseWesternblotWesternblotanalysisofα1B-adrenoreceptorinlysatesofratbrain(lanes1and3),ratkidney(lanes2and4)andGH3cellline(lanes5and6):1,2,5.Anti-α1B-AdrenergicReceptor(extracellular)Antibody(#AAR-018),(1:200).3,4,6.Anti-α1B-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.

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