Cat#:AAR-004LyophilizedPowderLyophilizedPowderThisproductisfreezedried.Allwatermoleculeshavebeenremoved.AntigenIncl.AntigenIncl.ThisantibodyisshippedwithitsantigenFREEofcharge!ShippedatRoomTemp.ShippedatRoomTemp.Thislyophilizedproductisshippedatroomtemperature.Pleaseseeitscertificateofanalysisforfurtherstorageinstructions.Type:PolyclonalSource:RabbitReactivity:h,rMayalsoworkin:mImmunogenPeptide(C)KETGAFYGREFKTAK,correspondingtoaminoacidresidues216-230ofhumanA3AR(AccessionP0DMS8).3rdintracellularloop.Accession(Uniprot)NumberP0DMS8GeneID140PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.HomologyRat,mouse -14/16aminoacidresiduesidentical.PurityAffinitypurifiedonimmobilizedantigen.FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.Reconstitution25μl,50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.Antibodyconcentrationafterreconstitution0.7mg/ml.StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.Controlantigenreconstitution100µldoubledistilledwater(DDW).Controlantigenstorageafterreconstitution-20°C.PreadsorptionControl1μgpeptideper1μgantibody.StandardqualitycontrolofeachlotWesternblotanalysis.Applications:ic,ih,wbMayalsoworkin:ifc,ipWesternblotWesternblotanalysisofMalme-3Mcelllysate:1.Anti-Adenosine A3 ReceptorAntibody(#AAR-004),(1:200).2.Anti-AdenosineA3 ReceptorAntibody,preincubatedwiththecontrolpeptideantigen.ImmunohistochemistryExpressionofAdenosine A3 ReceptorinratlungImmunohistochemicalstainingofparaffin-embedded ratlungsectionsusingAnti-Adenosine A3 Receptor Antibody(#AAR-004),(1:50).Stainingisspecificforrespiratoryepithelialcellsofthebronchiole(arrows)andpneumonocytesinthealveolarwall(a).Hematoxilinisusedasthecounterstain.ImmunocytochemistryExpressionofAdenosine A3 ReceptorinahumanmelanomacelllineImmunocytochemicalstainingofparaformaldehyde-fixedandpermeABIlized humanmelanomacells(A2058).A,D.CellswerestainedusingAnti-Adenosine A3 Receptor Antibody(#AAR-004),(1:100),followedbygoatanti-rabbitAlexaFluor-555secondaryantibody.B,E.Nuclearfluorescencestainingofcellsusingthemembrane-permeableDNAdyeHoechst33342.C.MergedimageofpanelsAandB.F.MergedimageofpanelsDandE.ReferencesOkusa,M.D.(2002)Am.J.Physiol.RenalPhysiol.282,F10.Fredholm,B.B.etal.(2001)Pharmacol.Rev.53,527.Nakata,H.(1989)J.Biol.Chem.264,16545Baraldi,P.G.etal.(2006)Curr.Med.Chem.13,3467.Headrick,J.P.etal.(2003)Am.J.Physiol.HeartCirc.Physiol.285,H1797.MADI,L.etal.(2004)Clin.CancerRes.10,4472.Ochaion,A.etal.(2007)Ann.Rheum.Dis.66,446.ScientificbackgroundAdenosineisanendogenousnucleosidegeneratedlocallyintissuesunderconditionsofhypoxia,ischemia,orinflammation.Itmodulatesavarietyofphysiologicalfunctionsinmanytissuesincludingthebrainandheart.1,2 Adenosineexertsitsactionviafourspecificadenosinereceptors(alsonamedP1purinergicreceptors):AdenosineA1 Receptor (A1AR), AdenosineA2A Receptor (A2AAR),AdenosineA2B Receptor (A2BAR),and AdenosineA3 Receptor (A3AR).Thevariousadenosinereceptorscanbedistinguishedonthebasisoftheirdistinctmolecularstructures,distincttissuedistributions,anddifferentialselectivityforadenosineanalogs.1-4 AllareintegralmembraneproteinsandaremembersoftheGprotein-coupledreceptorsuperfamily.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularaminoterminus,acytoplasmiccarboxylterminus,andathirdintracellularloopimportantinbindingGproteins.1-3ExpressionofA3ARhasbeenreportedinthebrain,kidney,liver,andheart.Itplaysaroleinmodulationofcerebralischemia,asthma,andcellgrowth.Inaddition,recentstudieshaveestablishedacardioprotectiveroleforA3AR.5 ExpressionofA3ARwasreportedtobeelevatedincanceroustissuesaswellasinauto-immuneinflammatorydiseases.6,7 ApatenthasbeenfiledfortheuseofA3ARandA3ARagoNISTasadiagnosticMarkerfortherapeutictreatmentsofcancerandotherdiseases.Applicationkey:CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-WesternblotSpeciesreactivitykey:H-Human,M-Mouse,R-RatLyophilizedPowderControlAntigenIncludedClickheretoreceivea25µlfreetrialsample!CertificateofAnalysisMSDSLastupdate:26/02/2019AlomoneLabsispleasedtoofferahighlyspecificantibodydirectedagainstanintracellularepitopeofthehumanadenosineA3 receptor(A3AR).Anti-Adenosine A3 ReceptorAntibody(#AAR-004)canbeusedinwesternblotanalysis,aswellasimmunocytochemicalandimmunohistocemicalapplications,andhasbeendesignedtorecognizeA3ARfromhuman,rat,andmousesamples.Forresearchpurposesonly,notforhumanuseWesternblotWesternblotanalysisofMalme-3Mcelllysate:1.Anti-Adenosine A3 ReceptorAntibody(#AAR-004),(1:200).2.Anti-AdenosineA3 ReceptorAntibody,preincubatedwiththecontrolpeptideantigen.

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