Cat#:AAR-020LyophilizedPowderLyophilizedPowderThisproductisfreezedried.Allwatermoleculeshavebeenremoved.AntigenIncl.AntigenIncl.ThisantibodyisshippedwithitsantigenFREEofcharge!ShippedatRoomTemp.ShippedatRoomTemp.Thislyophilizedproductisshippedatroomtemperature.Pleaseseeitscertificateofanalysisforfurtherstorageinstructions.Type:PolyclonalSource:RabbitReactivity:m,rMayalsoworkin:hImmunogenPeptide(C)DAGNSSWNGTEAPG,correspondingtoaminoacidresidues7-20ofratα2A-adrenoceptor(AccessionP22909).Extracellular,N-terminus.Accession(Uniprot)NumberP22909GeneID25083PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.HomologyMouse-identical;human-13/14aminoacidresiduesidentical.PurityAffinitypurifiedonimmobilizedantigen.FormulationLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.Reconstitution25µl,50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.Antibodyconcentrationafterreconstitution0.85mg/ml.StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).ControlantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.Controlantigenreconstitution100µldoubledistilledwater(DDW).Controlantigenstorageafterreconstitution-20°C.PreadsorptionControl1μgpeptideper1μgantibody.StandardqualitycontrolofeachlotWesternblotanalysis.Applications:ih,wbMayalsoworkin:ic,ifc,ip,lciWesternblotWesternblotanalysisofmouse(lanes1and3)andrat(lanes2and4)brainlysates:1,2.Anti-α2A-AdrenergicReceptor(extracellular) Antibody(#AAR-020),(1:200).3,4.Anti-α2A-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.ImmunohistochemistryExpressionofα2A-AdrenoreceptorinratcerebellumImmunohistochemicalstainingofratcerebellumfreefloatingfrozensectionusingAnti-α2A-AdrenergicReceptor(extracellular) Antibody(#AAR-020).A.α2A-Adrenoreceptorstaining(green)appearsinprocessesofBergmannglia(whitearrows)inthemolecularlayer(ML).B.Stainingofthesamesectionwithmouseantiglialfibrillaryacidicprotein(GFAP)(red),demonstratesexpressioninbothBergmanngliaandingranulelayer(GL)astrocytes.C.MergedimageofpanelAandBdemonstratingthatα2A-adrenoreceptorexpressionisrestrictedtoBergmannglia.DAPIisusedasthecounterstain(blue).Expressionofα2A-AdrenoreceptorinrathypothalamusImmunohistochemicalstainingofratventromedialhypothalamusfreefloatingfrozensectionusingAnti-α2A-AdrenergicReceptor(extracellular) Antibody(#AAR-020),(1:250).A.α2A-Adrenoreceptorstaining(green)appearsinastrocyticfibers(arrows)originatingfromthewallofthe3rdventricle(3rdV).B.DAPIisusedasthecounterstain(blue).C.MergedimageofpanelsAandB.ReferencesIUPHARRECEPTORDATABASE|ADRENOCEPTORSPiascik,M.T.andPerez,D.M.(2001)J.Pharmacol.Exp.Ther.298,403.CivantosCalzada,B.andAleixandredeArtinano,A.(2001)Pharmacol.Res.44,195.Bylund,D.B.(2005)Br.J.Pharmacol.144,159.ScientificbackgroundAdrenergicreceptors(alsocalledadrenoceptors)arethereceptorsforthecatecholaminesadrenalineandnoradrenaline(calledepinephrineandnorepinephrineintheUnitedStates).Adrenalineandnoradrenalineplayimportantrolesinthecontrolofbloodpressure,myocardialcontractilerateandforce,airwayreactivity,andavarietyofmetabolicandcentralnervoussystemfunctions. AdrenergicreceptorsaremembersoftheG-proteincoupledreceptor(GPCR)superfamilyofmembraneproteins.Theyshareacommonstructureofsevenputativetransmembranedomains,anextracellularN-terminus,andacytoplasmicC-terminus.Adrenoceptorsaredividedintothreetypes:α1,α2 andβ-adrenoceptors.Eachtypeisfurtherdividedintoatleastthreesubtypes:α1A,α1B,α1D,α2A,α2B,α2C,β1,β2,β31,2.Adrenoceptors areexpressedinnearlyallperipheraltissuesandinthecentralnervoussystem1,2. Ingeneral,thepharmacologicalpropertiesofeachsubtypearequitehomogenousacrossdifferentspecies.However,thisisnotthecasefortheα2A subtypewhichwasfirstisolatedfromhumanplatelets3.Thissubtypeshowsdifferentpharmacologicalpropertiesfromthatofmouseandrat.Forthisreason,untilmoleculartechniquessignificantlyadvanced,itwasbelievedthathumanα2A andthatofmouseandrat(termedα2D)weretwodifferentsubtypes.Today,itisacceptedthatthesetwosubtypesareinfactonegeneproductandisgenerallytermedα2A.Oneofthemaindifferencesbetweenthissubtypefromthedifferentorganismsisitsaffinityforyohimbineandrauwolscine;ratandmouseα2A displayslessaffinitycomparedtohuman3,4.Applicationkey:CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-WesternblotSpeciesreactivitykey:H-Human,M-Mouse,R-RatLyophilizedPowderControlAntigenIncludedClickheretoreceivea25µlfreetrialsample!CertificateofAnalysisMSDSLastupdate:04/11/2018Anti-α2A-AdrenergicReceptor(extracellular)Antibody(#AAR-020)isahighlyspecificantibodydirectedagainstanepitopeoftheratprotein.Theantibodycanbeusedinwesternblotandimmunohistochemistryapplications.Ithasbeendesignedtorecognizeα2A-adrenoceptorfrommouse,rat,andhumansamples.Forresearchpurposesonly,notforhumanuseWesternblotWesternblotanalysisofmouse(lanes1and3)andrat(lanes2and4)brainlysates:1,2.Anti-α2A-AdrenergicReceptor(extracellular) Antibody(#AAR-020),(1:200).3,4.Anti-α2A-AdrenergicReceptor(extracellular)Antibody,preincubatedwiththecontrolpeptideantigen.

       Alomone Labs是您离子通道研究中的伙伴，是离子通道、G蛋白耦联受体和神经信号工具的主要供应商。我们专业生产基础科学研究中所需的一抗、小分子、蛋白质和多肽。Alomone Labs提供的如下生命科学领域研究方案享誉海内外：离子通道研究领域（一系列全面的离子通道研究产品线，包括标记和未标记的抗体及其相关调节因子）、G蛋白耦联受体研究领域（提供了一整套不断增长的GPCR抗体、受体激动剂和拮抗剂产品名单）、 神经信号通路研究（包括一些用于神经营养因子研究的最重要的抗体和生长因子）。
 

       Alomone成立于1989年,已经成为全世界科学家信赖的最全面的离子通道研究工具供应商。所有Alomone产品均进行了生物实验验证，以冻干粉形式提供，可确保产品生物活性和保质期。Alomone可提供多种包装规格，灵活方便。作为Alomone Labs在中国的区域总代理，蚂蚁淘科技有限公司将为中国客户提供最全面的Alomone Labs产品以及客户订制化服务。