提醒:代购产品,无质量问题不接受退换货,下单前请仔细核对信息。下单后请及时联系客服
核对商品价格,订单生效后再付款。
Smartox/Maurocalcine, a potent agonist of ryanodine receptors/07MAU001-00100/0.1mg
价格:
自营商城
解放采购
正品保障
及时交付
厂家直采
一站服务
货号:
品牌:
会员服务:
尊享会员价
贵宾专线
运费优惠
闪电退款
福利优惠
上门换新
友情提示
以上价格仅为参考,请联系客服询价。
免费咨询热线
4000-520-616
产品说明
Maurocalcine,acomponentofthevenomofScorpiomauruspalmatus,wasdiscoveredbyMichelDeWaard,co-founderofSmartox. Maurocalcine foldsaccordingtoaninhibitorcysteineknot.Suchas ImperatoxinA,Maurocalcine actsasahighaffinityagoNISTofthe type-1ryanodinereceptorexpressedinskeletalmuscleswithanaffinityinthe10nMrange. Maurocalcine inducesanincreaseinchannelopeningprobABIlityaccompaniedbysuddentransitionstolonglastingsubconductancestates. Maurocalcine hasalsobeencharacterizedasacellpenetratingpeptideanditspharmacologicalactivitycanbeobserveduponextracellularperfusion. Description:Productcode:07MAU001.Categories:Calciumchannels,Ryanodinereceptors.Tags:hadrucalcin,imperatoxin,ryanodine,RyR.AAsequence: Gly-Asp-Cys3-Leu-Pro-His-Leu-Lys-Leu-Cys10-Lys-Glu-Asn-Lys-Asp-Cys16-Cys17-Ser-Lys-Lys-Cys21-Lys-Arg-Arg-Gly-Thr-Asn-Ile-Glu-Lys-Arg-Cys32-Arg-OHDisulfidebonds: Cys3-Cys17,Cys10-Cys21 andCys16-Cys32Length(aa): 33Formula: C156H260N56O46S6MolecularWeight: 3858.8DaAppearance: WhitelyophilizedsolidSolubility: waterandsalinebufferCASnumber: notavailableSource: SyntheticPurityrate: >98%Reference:Redox-sensitivestimulationoftype-1ryanodinereceptorsbythescorpiontoxinmaurocalcine.CellCalciumThescorpiontoxinmaurocalcineactsasahighaffinityagonistofthetype-1ryanodinereceptorexpressedinskeletalmuscle.Here,weinvestigatedtheeffectsofthereducingagentdithiothreitolortheoxidizingreagentthimerosalontype-1ryanodinereceptorstimulationbymaurocalcine.MaurocalcineadditiontosarcoplasmicreticulumvesiclesactivelyloadedwithcalciumelicitedCa²⁺releasefromnativevesiclesandfromvesiclespre-incubatedwithdithiothreitol;thimerosaladditiontonativevesiclesafterCa²⁺uptakecompletionpreventedthisresponse.Maurocalcineenhancedequilibrium[³H]-ryanodinebindingtonativeandtodithiothreitol-treatedreticulumvesicles,andincreased5-foldtheapparentKiforMg²⁺inhibitionof[³H]-ryanodinebindingtonativevesicles.Singlecalciumreleasechannelsincorporatedinplanarlipidbilayersdisplayedalong-livedopensub-conductancestateaftermaurocalcineaddition.Thefractionaltimespentinthissub-conductancestatedecreasedwhenloweringcytoplasmic[Ca²⁺]from10μMto0.1μMoratcytoplasmic[Mg²⁺]≥30μM.At0.1μM[Ca²⁺],onlychannelsthatdisplayedpooractivationbyCa²⁺werereADIlyactivatedby5nMmaurocalcine;subsequentincubationwiththimerosalabolishedthesub-conductancestateinducedbymaurocalcine.Weinterprettheseresultsasanindicationthatmaurocalcineactsasamoreeffectivetype-1ryanodinereceptorchannelagonistunderreducingconditions.RonjatM, etal.(2013)Redox-sensitivestimulationoftype-1ryanodinereceptorsbythescorpiontoxinmaurocalcine. CellCalcium. PMID:23623374MaurocalcineinteractswiththecardiacryanodinereceptorwithoutinducingchannelmodificationWehavepreviouslyshownthatMCa(maurocalcine),atoxinfromthevenomofthescorpionMauruspalmatus,bindstoRyR1(type1ryanodinereceptor)andinducesstrongmodificationsofitsgatingbehaviour.Inthepresentstudy,weinvestigatedtheabilityofMCatobindtoandmodifythegatingprocessofcardiacRyR2.Byperformingpull-downexperimentsweshowthatMCainteractsdirectlywithRyR2withanapparentaffinityof150nM.ByexpressingdifferentdomainsofRyR2invitro,weshowthatMCabindstotwodomainsofRyR2,whicharehomologouswiththosepreviouslyidentifiedonRyR1.TheeffectofMCabindingtoRyR2wasthenevaluatedbythreedifferentapproaches:(i)[(3)H]ryanodinebindingexperiments,showingaveryweakeffectofMCa(upto1muM),(ii)Ca(2+)releasemeasurementsfromcardiacsarcoplasmicreticulumvesicles,showingthatMCaupto1muMisunabletoinduceCa(2+)release,and(iii)single-channelrecordings,showingthatMCahasnoeffectontheopenprobabilityorontheRyR2channelconductancelevel.Long-lastingopeningeventsofRyR2wereobservedinthepresenceofMCaonlywhentheioniccurrentdirectionwasoppositetothephysiologicaldirection,i.e.fromthecytoplasmicfaceofRyR2toitsluminalface.Therefore,despitetheconservedMCabindingabilityofRyR1andRyR2,functionalstudiesshowthat,incontrastwithwhatisobservedwithRyR1,MCadoesnotaffectthegatingpropertiesofRyR2.TheseresultshighlightadifferentroleoftheMCa-bindingdomainsinthegatingprocessofRyR1andRyR2.AltafajX, etal. (2007) Maurocalcineinteractswiththecardiacryanodinereceptorwithoutinducingchannelmodification.BiochemJ. PMID:17537000Chemicalsynthesisandcharacterizationofmaurocalcine,ascorpiontoxinthatactivatesCa(2+)releasechannel/ryanodinereceptorsMaurocalcineisanoveltoxinisolatedfromthevenomofthechactidscorpionScorpiomauruspalmatus.Itisa33-merbasicpeptidecross-linkedbythreedisulfidebridges,whichshares82%sequenceidentitywithimperatoxinA,ascorpiontoxinfromthevenomofPandinusimperator.Maurocalcineispeculiarintermsofstructuralpropertiessinceitdoesnotpossessanyconsensusmotifreportedsofarinotherscorpiontoxins.Duetoitslowconcentrationinvenom(0.5%oftheproteins),maurocalcinewaschemicallysynthesizedbymeansofanoptimizedsolid-phasemethod,andpurifiedafterfolding/oxidationbyusingbothC18reversed-phaseandionexchangehigh-pressureliquidchromatographies.Thesyntheticproduct(sMCa)wascharacterized.Thehalf-cystinepairingpatternofsMCawasidentifiedbyenzyme-basedcleavageandEdmansequencing.ThepairingswereCys3-Cys17,Cys10-Cys21,andCys16-Cys32.Invivo,thesMCawaslethaltomicefollowingintracerebroventricularinoculation(LD(50),20microg/mouse).Invitro,electrophysiologicalexperimentsbasedonrecordingsofsinglechannelsincorporatedintoplanarlipidbilayersshowedthatsMCapotentlyandreversIBLymodifieschannelgatingbehaviorofthetype1ryanodinereceptorbyinducingprominentsubconductancebehavior.Fajloun,Z., etal.(2000)Chemicalsynthesisandcharacterizationofmaurocalcine,ascorpiontoxinthatactivatesCa(2+)releasechannel/ryanodinereceptors, FEBSLett. PMID:10713267Anewfoldinthescorpiontoxinfamily,associatedwithanactivityonaryanodine-sensitivecalciumchannelWedeterminedthestructureinsolutionby(1)Htwo-dimensionalNMRofMaurocalcinefromthevenomofScorpiomaurus.Thistoxinhasbeendemonstratedtobeapotenteffectorofryanodyne-sensitivecalciumchannelfromskeletalmuscles.ThisisthefirstdescriptionofascorpiontoxinwhichfoldsfollowingtheInhibitorCystineKnotfold(ICK)alreadydescribedfornumeroustoxicandinhibitorypeptides,aswellasforvariousproteaseinhibitors.Itsthreedimensionalstructureconsistsofacompactdisulfide-bondedcorefromwhichemergeloopsandtheN-terminus.Adouble-strandedantiparallelbeta-sheetcomprisesresidues20-23and30-33.Athirdextendedstrand(residues9-11)isperpendiculartothebeta-sheet.MaurocalcinestructuremimicstheactivatingsegmentofthedihydropyridinereceptorII-IIIloopandisthereforepotentiallyusefulfordihydropyridinereceptor/ryanodinereceptorinteractionstudies.Mosbah,A., etal.(2000)Anewfoldinthescorpiontoxinfamily,associatedwithanactivityonaryanodine-sensitivecalciumchannel, Proteins. PMID:10861934
Smartox Biotechnology 是全球唯一一家专门生产动物毒液多肽毒素,用于细胞离子通道功能研究的生物医药公司。多肽毒素在生物制药领域具有重要的使用价值。Smartox Biotechnology 于 2009 年由来自 Grenoble 神经科学研究所 (Grenoble Institute of Neuroscience) 的 Michel de waard 博士创立, Smartox Biotechnology 专门研究动物毒液,制作合成多种毒液中的多肽成分(常称为毒素)。 De Waard 博士研究离子通道与毒素多肽的关系,尤其是鉴定、开发毒素多肽作为治疗性分子或细胞穿透肽 (cell penetrating peptides, CPP) 。其研究团队在毒液分离,药理性活性肽鉴定、富半胱氨酸肽定性、制作和优化等方面具有独特、丰富的经验。 2010 年, Smartox Biotechnolgy 被法国研究部 (Ministry of Research) 授予“新兴企业 OSEO 奖 (OSEO prize for emerging businesses) ”。
新品排行榜
1
Smartox//12IBX001-00500/0.5mg
2
Smartox/ω Conotoxin MVII...
3
Smartox/Selective blocker of Na<...
4
Smartox/Blocker of Kv...
5
Smartox/Selective blocker of N-t...
6
Smartox//PTF004-00010/0.01mg
7
Smartox/Blocker of Kv...
8
Smartox/ASIC1a selective blocker...
9
Smartox/Selective blocker of ins...
10
Smartox/Selective blocker of mec...
文章排行榜
1
Smartox Biotechnology smartox-biotech|产品详情|进口试剂采购网
2
smartox-biotech【上等代理】141029 smartox-biotech公司
3
Smartox/Huwentoxin-XVI is a selective blocker of N-type Ca2+ channels/HWT016-00100/0.1mg
4
Smartox/Blocker of Kv2 channels/SCT01-50010/5x.0.01mg
5
特硫平 Q | smartox-biotech代理
6
smartox 多肽毒素_化学试剂_行业专用试剂_生化与分子生物学用试剂...
7
Smartox/TTX-S selective blocker/08HWT002-00100/0.1mg
8
Smartox/TTX-S selective blocker/HWF002-00100/0.1mg
9
ω-芋螺毒素-GVIA | Smartox产品
10
海南毒素-III | Smartox