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ZYMO RESEARCH/Zymoclean Large Fragment DNA Recovery/100 Preps/D4046
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Highlights Quick (15 minute) recovery of large-sized DNA (e.g., genomic, plasmid [BAC/PAC], viral, phage, etc.) from agarose gels. Unique column design for low volume (≥ 10 µl) elution of ultra-pure, high-yield DNA. Eluted DNA is well-suited for use in endonuclease digestion, sequencing, labeling, PCR, etc. DescriptionThe Zymoclean Large Fragment DNA Recovery Kit is a gel DNA extraction kit that provides a streamlined method for the rapid purification and concentration of high-quality large-sized DNA from agarose gels. Simply add the specially formulated Agarose Dissolving Buffer (ADB) to the gel slice containing a DNA sample, let dissolve, and then transfer to the supplied Zymo-Spin IC-XL Column. There is no need for organic denaturants or chloroform. Instead, the product utilizes unique spin column technology to yield high-quality, purified DNA in just minutes. DNA purified using the Zymoclean Large Fragment DNA Recovery Kit is ideal for PCR, sequencing, endonuclease digestion, ligation, etc. The entire procedure typically takes about 15 minutes. Technical Specifications Applicable ForHigh-quality, purified DNA is ideal for sequencing ligation reactions, and any other sensitive downstream applicationsElution Volume≥ 10 µlEquipmentMicrocentrifugePurityHigh-quality, purified DNA is especially well-suited for sequencing and ligation reactionsSample SourceAgarose gels containing large DNA fragments of interestSample storageEluted DNA can be used immediately or stored at ≤ -20°CSize Range50 bp to 200 kbTypeLarge DNAYieldUp to 10 µg DNA Citations Reviews“The best advantage about this kit is that it is really quick to make it, and the total DNA recuperated have a good quality and it have a good concentration to follow the experiments.” -Ingrid M.“The ADB buffer and higher temperature used melted the gel quicker than previously used kits thus making DNA recovery using this kit more efficient and generally quicker than previously used kits.” -Kelly-Anne F. “The kit was very easy to follow and yielded good results. It was more affordable than the Qiagen Gel Extraction kits, but worked just as efficiently. Solid product“ -Thomas B (University of Tennessee, Knoxville)Read More“Protocols are very well written and Instructions easy to follow. Always had good results with your products.” -Simin H. (University of California, Irvine)“It"s a kit with a faster protocol and [has] a very good efficiency in quantity of DNA recovered. Also have a possibility to elute only in 6 µl of water. “ -Joao B, (Instituto de Tecnologia Química e Biológica)“I had tried three other kits to do a certain recovery before this, I was getting bad yields, the elution volume required was quite large, the protocols were not as easy to follow and they took longer to complete. I liked everything about this kit, it is simple, efficient and quick.The protocol is easy to follow and the yield was 4-5 times better than what I got from other kits.” -Wendy P. (University of Auckland)“I did a direct comparison with the Qiagen kit (which we normally use) on duplicate samples. I found the yields to be the same, but the high concentration from Zymo resulting from the low elution volume was extremely helpful for a downstream ligation. That ligation also showed that the DNA products from both kits performed exactly the same (within the resolution of a standard gel). The elution concentration is probably the best aspect. The reduced number of buffers and steps is also nice, as are the low-bind column containers. I"ll probably be using this kit from now on.” -Adam C. (University of Texas, Southwestern) Kit ComponentsCat #NameSizePrice C1001-50Collection Tubes50 Pack$15.00 D3004-4-4DNA Elution Buffer4 ml$10.00 D3004-4-1DNA Elution Buffer1 ml$11.00 D4003-2-24DNA Wash Buffer (Concentrate)24 ml$33.00 D4001-1-50ADB (Agarose Dissolving Buffer)50 ml$33.00 D4001-1-100ADB (Agarose Dissolving Buffer)100 ml$63.00 D4003-2-6DNA Wash Buffer (Concentrate)6 ml$10.00 C1002-50Zymo-Spin IC-XL50 Pack$66.00 C1002-25Zymo-Spin IC-XL25 Pack$34.00
ZYMO RESEARCH生物科技公司位于美国加州Orange County，创建于上世纪90年代初，主要研发和生产生物科技实验室使用的各种生物试剂、耗材、仪器。其DNA提取试剂盒系列产品以“快速、简便、超纯”的特点在众多生物科技产品中脱颖而出，独占鳌头。已经形成了覆盖美、英、德、法、日、韩等50多个国家的销售网络，销售额快速增长。使用其产品的实验论文在各种权威性科技期刊（NATURE 自然、PNAS 美国科学院院刊、StemCells 干细胞、Nucleic Acids Res核酸研究等）上发表。
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