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ZYMO RESEARCH/OneStep PCR Inhibitor Removal Kit/50 Preps/D6030
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产品说明
Highlights Removes PCR inhibitors such as polyphenolics, humic/fulvic acids, tannins, melanin, etc. from nucleic acid solutions to yield high quality DNA or RNA. Fast, one-step procedure for cleaning impure samples prior to PCR, sequencing, reverse transcription (RT), etc. DescriptionThe OneStep PCR Inhibitor Removal Kits are PCR inhibitor clean up kits that contain all the components needed for efficient removal of contaminants that can inhibit downstream enzymatic reactions (e.g. PCR and RT) from DNA and RNA preparations. The column matrices have been specifically designed for the efficient removal of polyphenolic compounds, humic/fulvic acids, tannins, melanin, etc. from the most impure DNA and RNA preparations. Sample clean-up is as simple as applying, spinning, and recovering a sample from the column. Technical Specifications Applicable ForCleaned samples are ready for PCR, sequencing, reverse transcription (RT), etc.Elution Volume50 - 200 µlEquipmentMicrocentrifugePurityHigh quality, enzymatic-reaction ready DNA/RNA products.Sample SourceDNA/RNA samples contaminated with enzymatic reaction inhibitors.Sample StorageEluted DNA/RNA can be used immediately or stored at ≤ -20°C.TypeDNA and RNAYield50 - 90% recoveryProduct FAQ Q1: What is the smallest input volume of DNA that can be added to the OneStep Inhibitor Removal kit? The smallest volume will be 50 ul. Q2: What is the largest input volume of DNA that can be added to the OneStep Inhibitor Removal kit? The OneStep PCR Inhibitor Removal Kit can process about 500 µl of sample at a time. However, the same column can be reused for the same sample. Q3: Can I centrifuge at a lower speed than 3,500 x g for the 96-well kit? No, the kit requires the speed to be at 3,500 x g. Centrifuging at lower speeds can result in inefficient sample elution. Citations Reviews“The ease.Holy cow, this thing was so freaking simple compared to similar products” -Christian H. (University of Georgia)“It was VERY fast and yielded the intended results...PCR products from previously non-amplifiable DNA.” -Tara N. (United States Agricultural Research Service) Kit ComponentsCat #NameSizePrice C1001-50Collection Tubes50 Pack$15.00 D6035-1-30Prep Solution30 ml$18.00 C1058-50Zymo-Spin III-HRC Filters50 Pack$108.00 C2003Elution Plate2 Plates$19.00 C2009Silicon-A-HRC Plate2 Plates$405.00
ZYMO RESEARCH生物科技公司位于美国加州Orange County，创建于上世纪90年代初，主要研发和生产生物科技实验室使用的各种生物试剂、耗材、仪器。其DNA提取试剂盒系列产品以“快速、简便、超纯”的特点在众多生物科技产品中脱颖而出，独占鳌头。已经形成了覆盖美、英、德、法、日、韩等50多个国家的销售网络，销售额快速增长。使用其产品的实验论文在各种权威性科技期刊（NATURE 自然、PNAS 美国科学院院刊、StemCells 干细胞、Nucleic Acids Res核酸研究等）上发表。
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