Highlights Easy purification of high-quality DNA from whole blood, buffy coat, swabs, or cultured cells. Protocol excludes the use of Proteinase K and organic denaturants for biofluid and cell samples. Eluted, inhibitor-free DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc. DescriptionThe Quick-DNA Kits are ideal DNA isolation kits for easy, rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. Whole blood (fresh or stored), buffy coat, buccal cells, cells from culture, saliva, and other biological liquid samples can be processed with these kits.Zymo-Spin Column/Plate technology enables high-quality DNA purification in minutes.PCR inhibitors are effectively removed, and the eluted DNA is ideal for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications. Technical Specifications Elution Volume≥50 µlEquipmentMicrocentrifuge, VortexPurityHigh-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280>1.8Sample SourceWhole blood, plasma, or serum from humans, mice, rats, etc. Cells from culture, buccal cells, as well as a variety of biological liquids are effectively processed using this kit. Tissue already digested with Proteinase K or mechanically homogenized.Size RangeCapable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recoveredWorkflowUnique lysis buffer system omits the need for Proteinase K digestion for biological fluids and cell culture samples.YieldUp to 25 µg total DNA is eluted into ≥50 µl (30 µl minimum) DNA Elution Buffer or water. Human whole blood yields 3-7 µg DNA per 100 µl blood sampled. Mammalian tissues already homogenized will yield 1-3 µg DNA per mg.Product FAQ Q1: What is the difference between Quick-DNA and Quick-DNA Plus kits? The Quick-DNA is optimized for cells, soft tissues, and homogenized/digested samples using a single lysis/binding buffer. The Quick-DNA Plus kits contain an optimized Proteinase K for processing a wider variety of sample inputs, such as cells, blood, tissues, etc. The upgraded Quick-DNA Plus typically recovers more DNA. Q2: I’m seeing some yield inconsistencies with my blood samples, what’s happening? White blood cells, which are the major source of genomic DNA in blood, easily and quickly settles. Mix the blood sample well prior to taking an aliquot for purification. Q3: Can the Quick-DNA kit be used with bacterial samples? E.coli cells are easy-to-lyse and can be processed directly. For other microbes, additional pretreatment (e.g. enzymatic digestion or mechanical lysis) can be implemented and then processed with the Quick-DNA Kit. Alternatively, for an all-inclusive kit to process all microbes, use any of Zymo Research’s Environmental Kits (e.g. Quick-DNA Fungal/Bacterial, Quick-DNA Fecal/Soil, ZymoBIOMICS DNA, etc.) for DNA isolation. Q4: Can I use the Quick-DNA kit to clean-up previously isolated DNA? No, the kit is designed for direct use with biological samples. For clean-up of isolated DNA, please use the Genomic DNA Clean & Concentrator or the DNA Clean & Concentrator kits. Q5: Can Quick-DNA process crude lysates? Yes, add 4 volumes of Genomic Lysis Buffer to 1 volume of crude lysate, homogenized, or digested sample (see Cell Suspensions and Proteinase K Digested Samples) and proceed with the remainder of the protocol. Q6: What is the purpose of adding beta-mercaptoethanol? Can this step be substituted or omitted? Beta-mercaptoethanol is a reducing agent that helps break down proteins and improves DNA recovery and purity. Addition of beta-mercaptoethanol is recommended to enhance sample lysis, but can be substituted with dithiothreitol (DTT, final concentration of 10 mM) or omitted. Q7: Is it possible to add an RNase A treatment to the protocol? The Quick-DNA kits recover RNA-free genomic DNA. The selective chemistry allows for binding of double stranded DNA to the column and for RNA to flow through. No RNase A treatment is required when processing samples within kit specifications. Q8: What are the expected yields for each sample type? Keep in mind that there is sample to sample variability. Sample TypeInput Amount Expected YieldEukaryotic Cells1x106 Cells 5-6 µgSkeletal, Heart, Lung, Brain Tissue1 mg 1-3 µgLiver and Kidney Tissue1 mg 3-5 µgHuman Whole Blood100 µl 5-7 µg Citations Reviews“It was easy to work with, protocol easy to follow” -Tinatin T.“This kit did a good job of prepping clean genomic DNA.” -Tara N. (United States Agricultural Research Service)"This product was amazing! I"ve used the same type of kit (quick DNA extract) from Sigma and this was far more superior. I used the same amount of postnatal tissue as I would have for the Sigma kit, however the yield I obtained from Zymo was quite astounding considering the time of tissue digestion. Secondly, the gDNA was much "cleaner" upon measurement with our NanoDrop! " -Stephen C. (Johns Hopkins University School of Medicine) Kit ComponentsCat #NameSizePrice C1011-50Zymo-Spin IIC Columns50 Pack$55.00 C1001-50Collection Tubes50 Pack$15.00 D3004-5-50DNA Pre-Wash Buffer50 ml$26.00 D3004-5-15DNA Pre-Wash Buffer15 ml$10.00 D3004-1-50Genomic Lysis Buffer50 ml$34.00 D3004-1-100Genomic Lysis Buffer100 ml$60.00 D3004-2-50g-DNA Wash Buffer50 ml$18.00 D3004-2-100g-DNA Wash Buffer100 ml$30.00 D3004-4-10DNA Elution Buffer10 ml$14.00