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ZYMO RESEARCH/ZR-96 Genomic DNA Clean & Concentrator-5 Kit/4 x 96 Preps/D4066
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Highlights Quick, high-throughput (96-well) recovery of large-sized DNA from any enzymatic reaction or impure preparation without messy precipitations UniqueUnique spin column for low volume elution of ultra-pure, high-yield DNA. spin column for low volume elution of ultra-pure, high-yield DNA Eluted DNA is ideal for PCR, endonuclease digestion, sequencing, etc. DescriptionThe ZR-96 Genomic DNA Clean & Concentrator (DCC) is a 96-well gDNA clean up kit that provides quick (5 minute) recovery of ultra-pure, large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, (wga) DNA, etc.) from any enzymatic reaction or impure preparation (e.g., Proteinase K digestion). There is no need for organic denaturants, chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Buffer to a sample and then transfer the mixture to the supplied Zymo-Spin Column. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. This 96-well gDNA clean up kit is also compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions, crude plasmid preparations, cDNA synthesis, etc. Technical Specifications Applicable ForEluted DNA is ideal for ligation, sequencing, labeling, PCR, microarray, transfection, transformation, restriction digestion procedures, and any other sensitive downstream applicationElution Volume≥ 15 µl of DNA Elution BufferEquipmentCentrifuge w/ microplate carriersPurityA260/A280 > 1.8, A260/A230 > 1.8Sample SourceEnzymatic reactions or impure preparations containing genomic DNASample storageEluted DNA can be used immediately or stored at ≤ -20°CSize Range> 50 bp and up to 200 kbYieldUp to 5 µg DNA. Recovery of DNA ranges from 70 - 95%Product FAQ Q1: What is the lower limit and minimal amount of DNA that can be recovered? Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method. Q2: How to process naked DNA stored in DNA/RNA Shield? Use the standard protocol(add 2 or 5 volumes of DNA binding buffer depending on DNA size). Q3: What to do if ethanol addition to the DNA Wash Buffer was omitted? The DNA will be eluted off the column. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer. Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity? Oversaturation of the column can result in total DNA loss due to clogging of silica matrix. Q5: How many times can columns be reloaded? We recommend no more than 5 times as binding efficiency might decrease. Q6: What is the minimum input volume of DNA sample? Working with volumes below 50 µl can result in decreased recovery. We recommend raising the starting volume to 100 µl with water to ensure optimal binding conditions. Citations Kit ComponentsCat #NameSizePrice D3004-4-4DNA Elution Buffer4 ml$10.00 D3004-4-1DNA Elution Buffer1 ml$11.00 D3004-4-16DNA Elution Buffer16 ml$18.00 D3004-4-10DNA Elution Buffer10 ml$14.00 D4003-2-24DNA Wash Buffer (Concentrate)24 ml$33.00 D4003-2-6DNA Wash Buffer (Concentrate)6 ml$10.00 D4003-2-48DNA Wash Buffer (Concentrate)48 ml$60.00 D5201-1-50ChIP DNA Binding Buffer50 ml$37.00 D5201-1-100ChIP DNA Binding Buffer100 ml$83.00 C2010Zymo-Spin I-96-XL Plates2 Plates$257.00 C2003Elution Plate2 Plates$19.00 C2002Collection Plate2 Plates$22.00
ZYMO RESEARCH生物科技公司位于美国加州Orange County，创建于上世纪90年代初，主要研发和生产生物科技实验室使用的各种生物试剂、耗材、仪器。其DNA提取试剂盒系列产品以“快速、简便、超纯”的特点在众多生物科技产品中脱颖而出，独占鳌头。已经形成了覆盖美、英、德、法、日、韩等50多个国家的销售网络，销售额快速增长。使用其产品的实验论文在各种权威性科技期刊（NATURE 自然、PNAS 美国科学院院刊、StemCells 干细胞、Nucleic Acids Res核酸研究等）上发表。
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