class="ie9 no-js" lang="en"> DNA Clean & Concentrator-25 DNA Clean & Concentrator-25– ZYMO RESEARCHModal×.modal-body {padding: 2rem;}@media only screen and (max-width: 576px) {#global-modal-center-title {font-size: 28px;}.modal-body {padding: 1rem;}} #SearchDrawer {display: none;position: absolute;-webkit-overflow-scrolling: touch;z-index: 100;background-color: #fff;padding: 0 27.5px;}.search__input:focus {outline: none;}.search-bar__table-cell {display: table-cell;vertical-align: middle;}.search-bar__table {display: table;table-layout: fixed;width: 100%; height: 100%;}/*=============== Site Navbar Search Drawer =========================================*/.search-drawer--search__bar {text-align: center;}.search-drawer__form-wrapper {padding: 10px 0;width: 90%;}.search-drawer-search__input {max-width: 650px;width: 90%;border-radius: 25px;}.search-bar-drawer__off-click {opacity: 0;background-color: rgba(0,0,0,0.2);transition: opacity 0.3s;-webkit-transition: opacity 0.3s;}.search-bar-drawer__off-click--active {display: block;height: 100%;width: 100%;position: fixed;top: 0;z-index: 99;opacity: 1;}#search__submit_header > .icon-search {width: 20px;height: 20px;}@media only screen and (max-width: 576px) {.search-drawer-search__input {width: 80%;}}.ajax-loading-icon{position: absolute;margin-top: 70px;width: 40px;z-index:6;}.ui-autocomplete{background-color:#fff;border-radius:10px;top:-30px;box-shadow:0px 8px 25px rgba(0,0,0,.3);max-height:360px;overflow-y: auto;/* prevent horizontal scrollbar */overflow-x: hidden;/* add padding to account for vertical scrollbar */z-index:5;}.ui-helper-hidden-accessible {border: 0;clip: rect(0 0 0 0);height: 1px;margin: -1px;overflow: hidden;padding: 0;position: absolute;width: 1px;}.ui-menu-item{padding:10px 20px;border-top:1px solid #ececec;cursor:pointer;border-radius:10px;display:table;width:100%;}.ui-menu {padding-left: 0;}.ui-menu-item:first-child{border-top:none;}.ui-menu-item:hover{background-color:#fff3b4;}.sku-text{color: #bababa;font-size: 14px;margin: 5px 0;}.img-result {max-height: 100px;float: right;}.ui-menu-descrip {color: #69727b;font-size: 14px;line-height: 1.5;}#SearchDrawer > .ui-autocomplete{width: 100%;max-width: 650px;position: fixed;max-height: 600px;padding-left: 0;}.drawer{overflow:visible;}@media(max-width:767px){#SearchDrawer > .ui-autocomplete{left: 0 !important;max-width:100%;}}PRODUCTSSERVICESDISTRIBUTORSRESOURCESABOUT Welcome, Guest! Login RegisterPRODUCTSPRODUCTS MenuAllProductsDNARNAEpigeneticsMicrobiomicsE. coli YeastProtein & EnzymesSample CollectionColumns & PlasticsDevices & InstrumentsCustom SolutionsSERVICESSERVICES MenuAll ServicesEpigenetics Aging ClockDNA MethylationMicrobiomicsTargeted SequencingAdditional ServicesDISTRIBUTORSRESOURCESRESOURCES MenuBlogWhat is EpigeneticsBisulfite Beginner GuideLiteratureCertificate of AnalysisQuick OrderVideo LibraryABOUTABOUT MenuCompany ProfileAdvisory BoardContactOrderingEventsPress ReleasesHistory & StoriesAll ProductsDNARNAEpigeneticsMicrobiomicsE. coli YeastProtein & EnzymesSample CollectionColumns & PlasticsDevices & InstrumentsCustom SolutionsAll ServicesEpigenetics Aging ClockDNA MethylationTargeted SequencingMicrobiomicsRNA-SeqATAC-SeqChIP-SeqBlogWhat is EpigeneticsBisulfite Beginner GuideLiteratureCertificate of AnalysisQuick OrderVideo LibraryCompany ProfileAdvisory BoardContactOrderingEventsPress ReleasesHistory & Stories.sub-navigation{position: absolute; padding: 8px 20px; z-index: 5; background-color: #00843D; width: 100%; display: none;}.subnav-list{display: flex; justify-content: space-around; width: 100%; margin: 0 auto;list-style: none;flex-wrap: wrap;}.child-nav-label{margin: 0px 10px;color: #fff;font-size: 13px;}.child-nav-label:hover{color: #FEDD00;}.subnav-container{}.subnav-scroll-hide{}.site-nav__child-link {white-space: nowrap;padding: 0px;display: inline-block;margin: 5px 0;}@media (max-width: 992px) {.sub-navigation {display: none !important;}}Login×Email AddressPasswordForgot PasswordRegister×First NameLast NameEmailPasswordConfirm PasswordReset Your Password×Email Address.modal-title {font-size: 32px;}.nav-modal__input {width: 100%;margin-bottom: 20px;}.recover-password-anchor {display: block;padding-top: 10px;color: #00843d;cursor: pointer;margin-bottom: 0;}.nav__anchor {cursor: pointer;}#nav-register__confirm-pass,#confirm-password-btn {margin-top: 10px;}.form--error {color: #dc0000;background-color: #fff0f0;border-color: #dc0000;padding: 0.5em;}.global-site-navigation-bar {height: 75px;}.site-nav {background-color: white;box-shadow: 0px 2px 6px 0px rgba(0,0,0,0.2);}.navbar-brand {width: 175px;}.site-logo {width: 135px;height: 100%;}.nav-link-label {font-size: 13px;line-height: 1.2;overflow-wrap: break-word;word-wrap: break-word;color: #00843d;font-weight: 700;}.navbar-nav {width: 100%;}.nav-link {color: #00843d;}.nav-link .icon-chevron-down {width: 8px;height: 8px;margin-left: 0.5rem;}.icon-user-circle-solid {background-color: #FFF;border-radius: 30px;}.nav-btn--link {background-color: transparent;border: 0;margin: 0;color: #00843d;text-align: left;}.icon {display: inline-block;width: 20px;height: 20px;vertical-align: middle;fill: currentColor;}.site-header__icon {padding: 2px 5px;}.site-header__icon svg {height: 23px;width: 22px;}.site-header__account {color: #00843d;}.site-header__account-menu {display: none;position: absolute;z-index: 6;background-color: white;text-align: left;border-radius: 15px;padding: 15px 20px;border: 2px solid #00843D;top: 60px;width: 200px;}.site-header__cart-count {display: flex;align-items: center;justify-content: center;position: absolute;font-weight: bold;border-radius: 50%;min-width: 18px;height: 18px;right: -4px;top: 0;background-color: #FEDD00;color: #000;padding: 8px 6px;}@media only screen and (max-width: 1200px) {.site-header__account-menu {right: 20px;}}@media only screen and (max-width: 762px) {.site-header__account {display: none;}.site-header__account-menu.display-block {display: none;}}@media only screen and (max-width: 400px) {.nav-xsm-px-0 {padding-left: 0 !important;padding-right: 0 !important;}.site-logo {margin-left: -24px;width: 152px;}}/* Mobbile Side Nav */.mobile-nav-wrapper {min-height: 100vh;}.main-body-content,.flex-header-content {transition:transform 0.6s ease-out;}.main-body-content__close {overflow:hidden;transition:transform 0.6s ease-out;margin-right:auto;transform: translate(-80%);}.mobile-nav__close {-webkit-transform: translateX(112%);transform: translateX(112%);width: 90%;overflow-y: scroll;max-height: 100vh;display: none;position: absolute;margin-left: -5px;background-color: white;}.mobile-nav__dropdown {list-style: none;}.mobile-nav__item > .mobile-nav__link,.mobile-nav-parent__dropdown > .mobile-nav__label {color: #69727b;}.mobile-nav__label {border-bottom: 0;font-weight: 700;}.mobile-nav__off-click {opacity: 0;background-color: rgba(0,0,0,0.2);transition: opacity 0.3s;-webkit-transition: opacity 0.3s;width: 100%;}.mobile-nav__off-click--active {display: block;height: 100%;width: 20%;position: fixed;top: -30px;z-index: 99;right: 0px;opacity: 1;}.mobile-nav {display: block;-ms-transform: translate3d(0, 0, 0);-webkit-transform: translate3d(0, 0, 0);transform: translate3d(0, 0, 0);transition: all 0.45s cubic-bezier(0.29, 0.63, 0.44, 1);list-style: none;padding-left: 0;}.mobile-nav__link,.mobile-nav__sublist-link {display: block;width: 100%;padding: 15px 30px;font-size: 16px;padding-left: 0;}/* Space Filler Styles */.space-filler {height: 75px;}DNA Clean & Concentrator Kits (DCC) › DNA Clean & Concentrator-25.breadcrumb > a:not([disabled]):hover{color:#FEdd00;}.green-bg {background-color: #00843D;}.breadcrumb {color: white;font-weight: bold;padding: 10px 0;background-color: transparent;margin-bottom: 0;}.divider {margin: 0 5px;}DescriptionTech Specs FAQs Video Citations ReviewsComponentsDescriptionTech Specs FAQs VideoCitations ReviewsComponents.selector-wrapper select,.product-variants select {margin-bottom: 13px;} Request Samples×Only 3 free samples are allowed per order.Add Samples to CartLaunch demo modalThe sample has successfully been added to your cartGo To CartClose/* center popup sample modal */#sampleModal .modal-dialog {-webkit-transform: translate(0,-50%);-o-transform: translate(0,-50%);transform: translate(0,-50%);top: 47%;}#sampleModal .btn {width: 200px;}.samples-recv-btn,.request-sample-disabled,label.request-sample-disabled {cursor: not-allowed;opacity: .4;}.request-sample {font-size: 12px;padding: 5px 15px;margin-top: -10px;}#request-sample-bootstrap-override{color: #00843D;}.modal-body {padding: 2rem;}@media only screen and (max-width: 576px) {#add-multi-sample {font-size: 11px;}}@media only screen and (max-width: 1200px) {.sample-request__btn--mobile {float: right;margin: -50px 0;}}@media only screen and (max-width: 880px) {.sample-request__btn--mobile {margin: 0;float: none;display: block;}} DNA Clean & Concentrator-25DNA Clean & Concentrator-25Cat #NameSize PriceQuantityD4033DNA Clean & Concentrator-25 (Capped)50 Preps$75.00-+D4034DNA Clean & Concentrator-25 (Capped)200 Preps$272.00-+D4005DNA Clean & Concentrator-25 (Uncapped)50 Preps$75.00-+D4006DNA Clean & Concentrator-25 (Uncapped)200 Preps$272.00-+ ADD TO CARTDocuments.product-attachments {text-align: justify;} Highlights Quick (2 minute) desalting and recovery of ultra-pure DNA from enzymatic reactions (e.g., PCR and endonuclease digestions), cell-free lysates, etc. Column design allows DNA to be eluted at high concentrations into minimal volumes. Eluted DNA is well-suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc. DescriptionThe DNA Clean & Concentrator-25 (DCC-25) is a PCR purification kit designed for rapid desalting and purification of up to 25 µg DNA from enzymatic reactions (e.g., PCR), endonuclease digestions, or cell-free lysates. Simply add the specially formulated DNA Binding Buffer to your sample and transfer to the supplied Zymo-Spin column. This PCR purification kit features Zymo-Spin column technology to yield high-quality, purified DNA in just minutes, and it is compatible with cDNA and ssDNA. Eluted DNA is suitable for sequencing, microarray analysis, PCR, nucleotide blotting, and restriction endonuclease digestion procedures. Technical Specifications Detergent Tolerance≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDSElution Volume≥ 25 µl EquipmentMicrocentrifugePurityHighly-pure DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions.Sample Source DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc.Size Range50 bp to 23 kbYield≤ 25 µg total DNA can be recovered.For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%.Product FAQ Q1: What is the difference between capped and uncapped? The only difference is the cap. Everything else between the columns (max capacity, column matrix, etc.) is the same. Unsure which to use? It mainly comes down to preference. Some people like the capped columns for easy labeling or added security while others like the uncapped columns for faster sample processing. Q2: What is the minimum input volume of DNA sample? Working with volumes below 50 µl can result in decreased recovery. Zymo Research recommends raising the starting volume to 100 µl with water to ensure optimal binding conditions. Q3: How many times can columns be reloaded? Zymo Research recommends reloading columns no more than 5 times, as binding efficiency might decrease. Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity? Oversaturation of the column can result in total DNA loss due to clogging of silica matrix. Q5: How can I process naked DNA stored in DNA/RNA Shield? Use the standard protocol (add 2 or 5 volumes of DNA binding buffer depending on DNA size). Q6: What is the lower limit and minimal amount of DNA that can be recovered? Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method. Q7: What should I do if I did not add ethanol to the DNA Wash Buffer before starting? The DNA will be eluted off the column during the wash step. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.Product Video Citations Reviews“This kit performed as described. I was able to obtain good quality and yield of the DNA -better than with a couple of competitor"s kits.” -Kathleen B. (SUNY-ESF)“The simple steps and washing instructions were excellent. DNA was ready for use for PCR and gave strong results.” -Tyler C.“It only took 2 minutes for the total procedure - shorter than my current method of PCR purification.” -Marissa V. (Harvard Medical School) Read More“Almost no loss of elution buffer at the final step. It is quite impressive compared to the other kits I have used, which lose about 5-7µl.” -Takashi K. (Monash University)“I like the enhanced design of the column as it funnels every drop of volume directly through the silica in a very efficient manner.The quick spin times and relative ease of use are also a big draw as it cuts down on processing time and allows the attack of subsequent enzymatic steps at a heightened pace.” -Joseph R. (Miller School of Medicine, University of Miami)“I sampled this kit next to a kit we already use. I had three of the same sample, two for the Zymo kit and one for the other. On one of the ones designated for your kit, I accidentally added the sample directly to the column filter, then added other reagents (I figured I might as well try it). The other I did as per the instructions. The one on which I goofed gave a similar purification and concentration as the other kit, while the one I did properly yielded approximately four times the DNA per µL as the other kit. I am very happy with this result, and when I rerun my samples, I plan to use your product.” -Rosalind P. (University of Arkansas for Medical Sciences)“The elution volume is low enough to concentrate any sample very well and the efficiency is not compromised at all.” -Shanshan L. (UT Southwestern) “It was a very simple procedure and it gave a concentration ten times the original amount.” -Kimberly M. (University of Pennsylvania) Kit ComponentsCat #NameSizePrice C1078-50Zymo-Spin IICR Columns50 Pack$55.00 C1078-250Zymo-Spin IICR Columns5 x 50 Pack$228.00 D4003-2-6DNA Wash Buffer (Concentrate)6 ml$10.00 D4004-1-LDNA Binding Buffer100 ml$57.00 D4003-1-LDNA Binding Buffer50 ml$33.00 D4003-2-24DNA Wash Buffer (Concentrate)24 ml$33.00 D3004-4-10DNA Elution Buffer10 ml$14.00 D3004-4-4DNA Elution Buffer4 ml$10.00 C1001-50Collection Tubes50 Pack$15.00 C1008-50Zymo-Spin II Columns50 Pack$54.00 C1008-250Zymo-Spin II Columns5 x 50 Pack$233.00 C1001-500Collection Tubes500 Pack$52.00 C1001-1000Collection Tubes1000 Pack$90.00 Sample Report Instructions×To View the Report, Please Follow These Steps:Extract all the contents of the Sample16SReport1.Zymo.zip file.Open the extracted folder and find the file "report.html".Open the "report.html" file in your browser of choice.Within the report, there are links to view all the analyses performed for the project.Download ReportClose@media only screen and (min-width: 1500px) {.icon-desc.sd-xl{display:block; }#main-content.shadow-cover{ opacity: 1;}}@media (min-width: 1200px) {.pdp-ordering {position: sticky;position: -webkit-sticky;top: 94px;float: right;border-radius: 15px;width: 50%;box-shadow: 0 5px 25px rgba(0, 0, 0, .25);}}@media (min-width: 768px) {.prod-list-img{height: 350px;}}.product-nav-side {position: fixed;float: left;margin-right: -50px;-webkit-touch-callout: none;/* iOS Safari */-webkit-user-select: none;/* Safari */-khtml-user-select: none;/* Konqueror HTML */-moz-user-select: none;/* Firefox */-ms-user-select: none;/* Internet Explorer/Edge */user-select: none;/* Non-prefixed version, currently supported by Chrome and Opera */top: 180px;left: 0;z-index: 5;}.product-nav ul {background-color: #00843D;}.product-nav li {background-color: #00843D;cursor: pointer;color:white;}.hide-xl { display: none;}.icon-desc{display:block; -webkit-transition: display 1s;/* For Safari 3.1 to 6.0 */transition: display 1s;}.product-nav li:hover {background-color: #007041;color: #FEDD00;}.product-nav i {margin-right: 10px;}#stickyHeader.product-nav{position:fixed;z-index: 6;top:75px;}#down-icon span {line-height: 29px;}.fa-check.mr-2 {margin-left: -24px;}.text-red {color: red;}.highlights-section, .table, .expand-div {font-size:14px;}/* Pop up notification */.pop-up-notification {position: fixed;z-index: 1;padding-top: 100px;left: 0;width: 100%;height: 100%;overflow: auto;background-color: rgba(0, 0, 0, 0.4);top: 0px;}.close:hover,.close:focus {color: #000;text-decoration: none;cursor: pointer;}#PageContainer {overflow: visible;}.ajaxifyCart--qty input {font-size: 14px;border-radius: 0;border: 0;background-color: transparent;}.prod-qty-adjuster {cursor: pointer;line-height: 14px;display: block;top: 0;bottom: 0;padding: 5px 0;width: 33px;text-align: center;overflow: hidden;-webkit-user-select: none;-moz-user-select: none;-ms-user-select: none;-o-user-select: none;user-select: none;-webkit-transition: all 0.2s ease-out;-moz-transition: all 0.2s ease-out;-ms-transition: all 0.2s ease-out;-o-transition: all 0.2s ease-out;transition: all 0.2s ease-out;}.ajaxifyCart--qty {max-width: 80px;z-index: 5;border-color: #69727b;position: relative;}.section-btn.collapsed i:before{content:"f055";}.section-btn i:before{content:"f056"; } .pdf-icon {width: 20px;}.expand-header:hover,.expand-header:active {background-color: rgba(254, 221, 0, .3);cursor: pointer;}.readMore {font-size: 12px;margin: 5px;margin-top: 0px;}.order-table {font-size: 14px;color: #69727b;margin-bottom: 0;}.order-table td,.order-table th {padding: 8px 10px;border: none;}.order-table td {border-top: 1px solid #ececec;}.order-table thead {background-color: #00843D;color: white;border-bottom: 2px solid #FEDD00;}#submit-table svg {margin-left: -5px;margin-right: 5px;}.faq-question {border-radius: 10px;background-color: #ececec;margin-top: 10px;padding:12px;}.faq-question:before {content: "";width: 0px;height: 0px;position: relative;border-left: 10px solid transparent;border-right: 10px solid transparent;border-top: 10px solid #ececec;border-bottom: 10px solid transparent;right: 20px;top: 6px;margin-right: -15px;}.kit-component-table {max-height: 500px;overflow-y: auto;overflow-x: auto;}.order-table form {margin: 0;}.prod-list-img {border-radius: 10px;}.ordering-table-column-size, .ordering-table-sku {width: 20%;}.spec-table td,.spec-table th {border: none;border-bottom: 1px solid rgba(0, 0, 0, .1);}.spec-table td:last-child {max-width: 419px;}.spec-table tr:last-child td,.spec-table tr:last-child th {border: none;}.spec-table th {background-color: #00843D;}.spec-table tr:first-child th {border-radius: 8px 8px 0 0;}.spec-table tr:last-child th {border-radius: 0 0 8px 8px;}.discontinued-banner {width: 100%;background: #c32035;color: #fff;text-align: center;padding: 15px;}.discontinued-banner a {color: lightgray;}.featured-citations-row { margin: 30px 0; }.featured-citations-type { font-weight: 700;font-size: 0.9em;}@media only screen and (max-width: 1500px) {#side-nav-list {-webkit-transition: margin 1s;/* For Safari 3.1 to 6.0 */transition: margin 1s;}#main-content.shadow-cover{ opacity: 0.4; pointer-events: none;}.product-nav-side {position: fixed;overflow-x: hidden;}#page-width {-webkit-transition: margin 1s;/* For Safari 3.1 to 6.0 */transition: margin 1s;padding: 16px;}.hide-xl {display: block;}.icon-desc.sd-xl{display:none;}}@media only screen and (max-width: 1200px) {.product-nav-side {top: 200px;}}@media (max-width: 767px) {.prod-list-img{height: 100%;width:100%;}}CompanyHomeCareersEventsScientific Advisory Board25th AnniversaryHelpOrderingContactResourcesBlog Privacy PolicyTerms of ServicePromotionsCertificate of AnalysisQuick OrderVideo LibrarySocialize © 2020 Zymo Researchfooter {color: #ffffff;background-color: #00843d;font-style: normal;bottom: 0;}.footer-icon {font-size: 20px;width: 30px;padding: 15px 10px;}footer h2,.footer-anchor {color: #fff;}footer .footer-anchor:hover {color: #e6e6e6;}hr.hr-color {background-color: rgba(255, 255, 255, .2);}.zymo-corp {font-size: 13px;color: #BADAC7;}.footer-section {border-top: 4px solid #FEDD00;}.footer-width {padding: 0 55px;}.footer-lists-container {max-width: 990px;}.footer-columns {min-width: 210px;}.footer-list-title {font-size: 1.125em;letter-spacing: .03em;}.footer-list-title,.footer-list li {width: -webkit-max-content;width: intrinsic;}ul.footer-list {list-style-type: none;padding-inline-start: 0;}@media only screen and (max-width: 990px) {.social-insta {justify-content: center;margin: auto;width: -webkit-fill-available;min-width: -webkit-fit-content;}.media-title {margin: 17.5px auto;}}@media only screen and (max-width: 768px) {.footer-icon {width: 43px;padding: 10px 7px;}}@media only screen and (max-width: 480px) {.footer-icon {width: auto;font-size: 13px;}}Chat with us,powered by LiveChat