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ZYMO RESEARCH/ZR-96 DNA Clean-Up Kit/4 x 96 Preps/D4017
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Highlights Quick (20 minute), large-scale recovery of ultra-pure DNA from PCR, endonuclease digestions, cell-free lysates, etc. ZR-96 Silicon-A Plate design allows DNA to be eluted at high concentrations into minimal volumes of solvent. Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, etc. DescriptionThe ZR-96 DNA Clean-up Kit is a PCR purification kit that provides for rapid, large-scale (96-well) purification and concentration of high-quality DNA from PCR samples, endonuclease digestions, or crude plasmid preparations. Simply add the specially formulated DNA Binding Buffer to your samples and transfer to the wells of the supplied Silicon-A Plate. There is no need for organic denaturants or chloroform. Instead, the product features Fast-Spin plate technology to yield high-quality, purified DNA in just minutes. Technical Specifications Detergent Tolerance≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDSElution Volume≥ 30 µl for shallow well, ≥ 10 µl for deep wellEquipmentCentrifuge with microplate carriersPurityA260/A280 > 1.8Sample Source DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc.Size Range75 bp to 23 kb for shallow well, 50 bp to 23 kb for deep wellYield≤ 5 µg total DNA can be recovered.For DNA 75 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%.Product FAQ Q1: What is the lower limit and minimal amount of DNA that can be recovered? Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method. Q2: How to process naked DNA stored in DNA/RNA Shield? Add an equal volume of ethanol (95-100%) to the sample and mix well. The sample is ready-to-bind and does not require DNA Binding Buffer. Proceed to Step 2. Q3: What to do if ethanol addition to the DNA Wash Buffer was omitted? The DNA will be eluted off the column. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer. Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity? Oversaturation of the column can result in total DNA loss due to clogging of silica matrix. Q5: How many times can columns be reloaded? We recommend no more than 5 times as binding efficiency might decrease. Q6: What is the minimum input volume of DNA sample? Working with volumes below 50 µl can result in decreased recovery. We recommend raising the starting volume to 100 µl with water to ensure optimal binding conditions. Citations Kit ComponentsCat #NameSizePrice D4003-2-24DNA Wash Buffer (Concentrate)24 ml$33.00 D4004-1-LDNA Binding Buffer100 ml$57.00 D4003-2-48DNA Wash Buffer (Concentrate)48 ml$60.00 C2001Silicon-A Plate2 Plates$129.00 C2003Elution Plate2 Plates$19.00 C2002Collection Plate2 Plates$22.00
ZYMO RESEARCH生物科技公司位于美国加州Orange County，创建于上世纪90年代初，主要研发和生产生物科技实验室使用的各种生物试剂、耗材、仪器。其DNA提取试剂盒系列产品以“快速、简便、超纯”的特点在众多生物科技产品中脱颖而出，独占鳌头。已经形成了覆盖美、英、德、法、日、韩等50多个国家的销售网络，销售额快速增长。使用其产品的实验论文在各种权威性科技期刊（NATURE 自然、PNAS 美国科学院院刊、StemCells 干细胞、Nucleic Acids Res核酸研究等）上发表。
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