首页 > Adipogen > Adipogen/HSP70/HSP40 (human) Protein Refolding Kit/AG-44T-0115-KI01/1 Kit

提醒：代购产品，无质量问题不接受退换货，下单前请仔细核对信息。下单后请及时联系客服核对商品价格，订单生效后再付款。

Adipogen/HSP70/HSP40 (human) Protein Refolding Kit/AG-44T-0115-KI01/1 Kit

价格：

登录查看

自营商城

解放采购

正品保障

及时交付

厂家直采

一站服务

货号：

AG-44T-0115-KI01

品牌：

Adipogen

会员服务：

尊享会员价

贵宾专线

运费优惠

闪电退款

福利优惠

上门换新

友情提示

以上价格仅为参考，请联系客服询价。

免费咨询热线

4000-520-616

产品说明

Specifications&Handling:PropertiesCrossreactivityHumanKitContains30μlof10XHSP70Solution30μlof10XHSP40Solution50μlof10XHSP70ReactionBuffer30μlof10XMg-ATPSolution50μlof10XGlow-Fold™SubstrateProtein2mlofLuciferinReagentNOTE:Kitcontainsreagentssufficientfor15x20μlreactions.OtherProductDataUse:ThiskitprovidesafunctionalinvitroHSP70/HSP40refoldingsystem.Usingtheprovidedsubstrateprotein,thekitcanbeusedtoscreenforsmallmoleculesaffectingtheefficiencyoftherefoldingprocess(suchasHSPinhibitors).Alternatively,theHSP70/HSP40complexmaybeusedtotestrefoldingofuser-suppliedproteinsifafunctionalassayisavailable.OriginManufacturedbyBostonBiochemProductTypeKitShippingandHandlingShippingDRYICEShortTermStorage-20°CLongTermStorage-80°CHandlingAdviceAliquottoavoidfreeze/thawcycles.Use/StABIlityStableforatleast1yearafterreceiptwhenstoredat-80°C.DocumentsManualDownloadDescription:Heatshockproteins(HSPs)areafamilyofhighlyconservedstressresponseproteins.Heatshockproteinsfunctionprimarilyasmolecularchaperonesbyfacilitatingthefoldingofothercellularproteins,preventingproteinaggregationortargetingimproperlyfoldedproteinstospecificdegradativepathways.HSP70functiondependsonitscyclingbetweentwostates:ATP-boundandADP-bound.ATP-boundHSP70recognizesstretchesofhydrophobicaminoacidresiduesinmisfoldedornewlysynthesizedproteinsandinteractswiththem.ThisinitialbindingeventisrelativelyweakandreversIBLe,andsotheATP-boundHSP70freelybindsandreleasepeptides.However,thepresenceofapeptideinthebindingdomainweaklystimulatesthelatentATPaseactivityofHSP70.OnceATPishydrolyzedtoADP,thebindingpocketofHSP70isreconfiguredintoatight-bindingstatethatclampsdownonproteintargetsandhelpstopreventtheiraggregation.ATPhydrolysisisstimulatedbyHSP70associationwith“J-domain”classco-chaperonessuchasHSP40.Theseco-chaperonesdramaticallyincreasetheactivityandthefunctionalityofHSP70inthepresenceofinteractingpeptides/proteins.EventuallyADPisexchangedforanATPmolecule,thusbringingthesubstrate-bindingdomainbacktoitslowaffinitystate.Thisreleasestheboundproteinsothatitmayfoldcorrectlybyitself,interactwithotherchaperonesystemstocompletefolding,orbindagaintoHSP70.

Adipogen公司由三个子公司组成，分别是Adipogen,Inc.(韩国),AdipogenSA(瑞士)和 AdipogenCorporation(美国圣地亚哥)。总部设在美国，运营总部在瑞士利斯塔尔。Adipogen公司专门从事生命科学研究试剂的开发，如癌症、免疫、炎症、代谢疾病（糖尿病、肥胖）、干细胞和神经退行性疾病等领域。其重点关注开发创新优质的ELISA试剂盒。另外，也可提供抗体和蛋白产品，Adipogen拥有自己的化学实验室，致力于开发创新型小分子化合物。