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Adipogen/IL-1α (mouse) ELISA Kit/AG-45B-0003-KI01/96 wells
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Specifications&Handling:ProductDataSynonymsInterleukin-1αIL-1F1PropertiesApplicationSetQuantitativeELISASpecificityDetectsnaturalandrecombinantmouseIL-1α(proformandp17fragment).DoesnotdetecthumanIL-1αormouseIL-1β.CrossreactivityMouseQuantity1x96wellsSensitivity9pg/mlRange15.6pg/mlto1ng/mlSampleTypeCellCultureSupernatant,Plasma,SerumAssayTypeSandwichDetectionTypeColorimetricProductTypeKitShippingandHandlingShippingBLUEICEShortTermStorage+4°CLongTermStorage+4°CHandlingAdviceAfterstandardreconstitution,preparealiquotsandstoreat-20°C.Avoidfreeze/thawcycles.Plateandreagentsshouldreachroomtemperaturebeforeuse.Use/StABIlity12monthsafterthedayofmanufacturing.SeeexpirydateonELISAKitbox.DocumentsManualDownloadMSDSDownloadRelatedProductsanti-IL-1α(p18)(mouse),mAb(Teo-1)anti-IL-1α(mouse),mAb(Bamboo-2)(Biotin)anti-IL-1α(mouse),mAb(Bamboo-2)IL-1α(mouse)MatchedPairDetectionSetanti-IL-1α(mouse),mAb(Bamboo-1)Description:Themostprominentmembersoftheinterleukin-1(IL-1)superfamilyareIL-1αandIL-1β.Theylackasignalpeptideandaresecretedbyanunconventional,endoplasmicreticulum-Golgi-independentmechanism.IL-1αwasreportedtobemorewidelyandconstitutivelyexpressedandhasintracellularfunctions,butalsoactslocallyinamembrane-boundformbyactivatingIL-1R1.Additionally,passivereleaseofIL-1αuponcelldeathcantriggerasterileinflammatoryresponsetodyingcells.ThecleavageofIL-1αisnotmediatedbycaspase-1andisnotrequiredforbindingtoIL-1R1.RecentlyithasbeenobservedthatallactivatorsoftheinflammasomeNLRP3/NALP3inducethesimultaneoussecretionofIL-1αandIL-1β.AlthoughmostactivatorsfullyrelyontheinflammasomeforIL-1αsecretion,someinducetheprocessingandsecretionofIL-1αinaninflammasome-independentmanner.
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