首页 > Adipogen > Adipogen/Ubiquitin Conjugation Kit/AG-44T-0132-KI01/1 Kit

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Adipogen/Ubiquitin Conjugation Kit/AG-44T-0132-KI01/1 Kit

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AG-44T-0132-KI01

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Adipogen

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Specifications&Handling:ProductDataSynonymsUBB;UbiquitinBPropertiesCrossreactivityRabbitKitContains200µgofConugationFractionA200µgofConugationFractionB50µlofUbiquitinSolution150µlof10XEnergySolutionNOTE:Thiskitsuppliesenoughmaterialsfor10x50µlreactions.OtherProductDataUse:Thiskitisfortheformationofubiquitinatedsubstrateproteins.Theenzymessuppliedrepresentthefullcomplimentofpurifiedconjugationenzymes(E1,E2s,andE3s)thatarefoundinFractionII.ConjugationFractionAcontainspredominantlyE1andE2enzymes,whileConjugationFractionBcontainspredominantlyE3anddeubiquitinatingenzymes.Theenzymeshavebeentestedandshowntoworkwithtypical[125I]-labeledsubstrateproteinssuchaslysozymeandβ-lactoglobulin.ThisconjugationfractionalsocontainsubiquitinC-terminalhydrolasesandthereforetheadditionofUbiquitinAldehyde(AG-40T-0511)isrecommendedfortheinhibitionofUCHstoimproveoverallconjugateyield.Thesuppliedfractionsdonotcontain20Sor26Sproteasomesoranyotherproteindegradationactivity.IfthesubstratebeingconjugatedrequiresE2orE3enzymesnotfoundinFractionII,thereactioncanbesupplementedwithenzymesfromFractionI(Prod.No.AG-40T-0344).OriginManufacturedbyBostonBiochemProductTypeKitShippingandHandlingShippingDRYICEShortTermStorage-20°CLongTermStorage-80°CHandlingAdviceAliquottoavoidfreeze/thawcycles.Use/StABIlityStableforatleast6monthsafterreceiptwhenstoredat-80°C.DocumentsManualDownloadDescription:Ubiquitinisa76aminoacid(aa)proteinthatisubiquitouslyexpressedinalleukaryoticorganisms.ubiquitinishighlyconservedwith96%aasequenceidentitysharedbetweenhumanandyeastubiquitin,and100%aasequenceidentitysharedbetweenhumanandmouseubiquitin.Inmammals,fourubiquitingenesencodefortwoubiquitin-ribosomalfusionproteinsandtwopoly-ubiquitinproteins.Cleavageoftheubiquitinprecursorsbydeubiquitinatingenzymesgivesrisetoidenticalubiquitinmonomerseachwithapredictedmolecularweightof8.6kDa.ConjugationofubiquitintotargetproteinsinvolvestheformationofanisopeptidebondbetweentheC-terminalglycineresidueofubiquitinandalysineresidueinthetargetprotein.Thisprocessofconjugation,referredtoasubiquitinationorubiquitylation,isamulti-stepprocessthatrequiresthreeenzymes:aubiquitin-activating(E1)enzyme,aubiquitin-conjugating(E2)enzyme,andaubiquitinligase(E3).ubiquitinationisclassicallyrecognizedasamechanismtotargetproteinsfordegradationandasaresult,ubiquitinwasoriginallynamedATP-dependentProteolysisFactor1(APF-1).Inadditiontoproteindegradation,ubiquitinationhasbeenshowntomediateavarietyofBIOLOGicalprocessessuchassignaltransduction,endocytosis,andpost-endocyticsorting.

Adipogen公司由三个子公司组成，分别是Adipogen,Inc.(韩国),AdipogenSA(瑞士)和 AdipogenCorporation(美国圣地亚哥)。总部设在美国，运营总部在瑞士利斯塔尔。Adipogen公司专门从事生命科学研究试剂的开发，如癌症、免疫、炎症、代谢疾病（糖尿病、肥胖）、干细胞和神经退行性疾病等领域。其重点关注开发创新优质的ELISA试剂盒。另外，也可提供抗体和蛋白产品，Adipogen拥有自己的化学实验室，致力于开发创新型小分子化合物。