MoreInformationProductDetailsSynonymsF-actinCross-linkingProteinProductTypePolyclonalAntibodyPropertiesSource/HostRabbitImmunogen/AntigenSyntheticpeptidecorrespondingtoaa27-38oftheN-terminalα-Actinin-4,coupledviaaC-terminalcysteineresiduetoKLH.ApplicationImmunocytochemistry:(1:250-1:1"600)Immunohistochemistry:(1:250-1:1"600)Immunoprecipitation:(1:10)WesternBlot:(1:1"000-1:2"500)Optimalconditionsmustbedeterminedindividuallyforeachapplication.CrossreactivityHamsterHumanMousePigRatSpecificityRecognizeshuman,mouse,rat,pigandChinesehamsteractinin-4.Doesnotcross-reactwithactinin-1,-2or-3.PurityAntigenaffinitypurified.Concentration250μg/mlFormulationLiquid.InPBScontaining1mg/mlBSAand0.01%sodiumazide.OtherProductDataContainsPositiveControl:Humanplateletprotein(Prod.No.AG-35T-0001),500μgsuppliedat5mg/mlinSDSsamplebuffer.Use5μl(25μg)perlaneforWesternblotting.AccessionNumberUniProtIDO43707:alpha-Actinin-4(human)ShippingandHandlingShippingBLUEICEShortTermStorage+4°CLongTermStorage-20°CHandlingAdviceAvoidfreeze/thawcycles.Use/StABIlityStableforatleast1yearafterreceiptwhenstoredat-20°C.DocumentsMSDSDownloadPDFProductSpecificationSheetDatasheetDownloadPDFα-Actinin4isanactin-bundlingproteinof~100kDathatisassociatedwithcellmotility,endocytosisandcancerinvasion.Theα-actininfamilycomprisestwonon-muscleisoforms(α-actinin-1and-4)andtwoskeletalmuscleisoforms(α-actinin-2and-3),withα-actinin-2beingalsoexpressedincardiacmuscle.Whileα-actinin-4isalmostubiquitouslyexpressed,particularlyhighconcentrationsarefoundinglomeruli.Onthesubcellularlevelitisassociatedwithactinstressfibers,butincertaincellsitalsolocalizestothenucleus.Mutationsintheα-actinin-4genecauseanautosomal-dominantformoffamilialfocalsegmentalglomerulosclerosis(FSGS),whichisthoughttoresultfromadefectinglomerularpodocytefunction.Apointmutationintheα-actinin-4genewasfoundtogenerateanantigenicpeptidethatisrecognizedbyautologouscytolyticTlymphocytes(CTL)onahumanlungcarcinoma.α-Actinin-4interactswithavarietyofproteins,includingtheringfingerproteinBERP,thePDZ-LIMproteinCLP-36,thehemidesmosomalandcell-cellcontactproteinBP180,andthetightjunctionproteinMAGI-1.Moreover,α-actinin-4formsaternarycomplexwithCa2+/Calmodulin-dependentproteinkinaseIIanddensin-180,aproteinofpostsynapticdensitiesinCNSneurons.Ca2+-dependentassociationofα-actinin-4withE3KARPisrequiredforCa2+-dependentinhibitionoftheNa+/H+exchanger3(NHE3).ProductReferencesExpressionofα-actinin-4inacquiredhumannephroticsyndrome:aquantitativeimmunoelectronmicroscopystudy:N.P.Goode,etal.;Nephrol.Dial.Transplant. 19,844(2004)TheRelationshipbetweenSubcellularLocalizationofActinin-4andCellMotilityinOralSquamousCellCarcinoma:Y. Iida&H.Chiba; OralSci.Intern.1,30(2004)ParietalPodocytesinNormalHumanGlomeruli:J.Beriety,etal.;JASN17,2770(2006)VASP-dependentregulationofactinCytoskeletonrigidity,celladhesion,anddetachment:A.B. Galler,etal.; Histochem.CellBiol.125,457(2006)Extra-cellularmatrixproteinsinducere-distributionofalpha-actinin-1andalpha-actinin-4inA431cells:A. Bolshakova,etal.; CellBiol.Int.31,360(2007)Alpha-actininisrequiredfortightlyregulatedremodelingoftheactincorticalnetworkduringcytokinesis:S.Mukhina,etal.; Dev.Cell13,554(2007)Expressionofalpha-actinin-4inhumandiabeticnephropathy:M.Kimura,etal.; Intern.Med.47,1099(2008)Expressionofα-Actinin-1inHumanGlomerularMesangialCellsInVivoandInVitro:C.Yang&W.F.Glass;  Exp.Biol.Med.233,689(2008)AnalysisofNuclearProteinComplexesComprisinga-Actinin-4by2D-ElectrophoresisandMassSpectrometry:M.G.Khotin,etal.;CellandTissueBIOLOGy3,431(2009)Autophagyinfluencesglomerulardiseasesusceptibilityandmaintainspodocytehomeostasisinagingmice:B.Hartleben,etal.;  J.Clin.Invest.120,1084(2010)Molecularmechanismsunderlyingnucleocytoplasmicshuttlingofactinin-4:M.Kumeta,etal.;J.CellSci.123,1020(2010)AlbuminuriaandGlomerularDamageinMiceLackingtheMetabotropicGlutamateReceptor1:A.Puliti,etal.;Am.J.Pathol.178,1257(2011)NephroticSyndromeandSubepithelialDepositsinaMouseModelofImmune-MediatedAnti-PodocyteGlomerulonephritis:C.Meyer-Schwesinger,etal.;J.Immunol.187,3218(2011)UbiquitinC-terminalhydrolase-l1activityinducespolyubiquitinaccumulationinpodocytesandincreasesproteinuriainratmembranousnephropathy:C.Meyer-Schwesinger,etal.; Am.J.Pathol.178,2044(2011)Calciumsensitivityofα-actininisrequiredforequatorialactinassemblyduringcytokinesis:R.Jayadev,etal.;CellCycle11,1929(2012)InteractionofG-proteinβγcomplexwithchromatinmodulatesGPCR-dependentgeneregulation:A.Bhatnagar,etal.;PLoSONE8,e52689(2013)