Real-Time PCR Enzymes & KitsProbe-Based One-Step qRT-PCR12Probe-Based qPCR13Reverse Transcription & cDNA Synthesis for qPCR8SYBR Green- or Dye-Based One-Step qRT-PCR6SYBR Green- or Dye-Based qPCR10Rotor-Gene SYBR® Green RT-PCR KitPrint For ultrafast, one-step qRT-PCR gene expression analysis using SYBR Green I on Rotor-Gene cyclers Optimized for ultrafast, reliable results on Rotor-Gene cyclersSensitive detection of even low copy numbersAccurate detection of a wide range of template amountsSpecially formulated, ready-to-use master mix for fast cyclingGuaranteed performance combined with QuantiTect Primer AssaysThe Rotor-Gene SYBR Green RT-PCR Kit is designed for use with the Rotor-Gene Qand other Rotor-Gene cyclers, providing ultrafast, highly specific quantification of RNA targets withreal-time one-step RT-PCR using SYBR Green I detection. Outstanding performance is achieved through the combination of a specially optimized master mix and the unique Rotor-Gene cycler. For convenience, the master mix can be stored at 2–8°C.Buy ProductsProduct DetailsSpecificationsProduct ResourcesBuy ProductsCat No./ID:204174Rotor-Gene SYBR Green RT-PCR Kit (400)$961.00Order ProductFor 400 x 25 µl reactions: 3 x 1.7 ml 2x Rotor-Gene SYBR Green RT-PCR Master Mix, 100 µl Rotor-Gene RT Mix, 2x2ml RNase-Free WaterThe Rotor-Gene SYBR® Green RT-PCR Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.Product Details Specific primer annealing.Fast primer annealing.Specific detection without the need for optimization.Tenfold dilutions of human leukocyte RNA (100 ng to 10pg) were used as template in SYBR® Green-based real-time one-step RT-PCR. Duplicate reactions were run using the QuantiTect Primer Assay for BCL2 (B-cell CLL/lymphoma 2). [A] The Rotor-Gene Q and Rotor-Gene SYBR® Green RT-PCR Kit provided sensitive detection from 10 pg RNA and amplification of specific PCR product (melting curve shown in inset). [B] In contrast, an instrument and kit from Supplier R provided detection only after optimization of Mg2+ concentration. However, the limit of detection was 100 pg RNA and coamplification of nonspecific products was observed (melting curve shown in inset).PerformanceWhen the Rotor-Gene SYBR Green RT-PCR Kit is used together with QuantiTect Primer Assays, highly sensitive quantification of specific PCR products is achieved without the need for optimization (see figures "Specific detection without the need for optimization").Principle The Rotor-Gene SYBR Green RT-PCR Kit enables reliable real-time RT-PCR quantification on the Rotor-Gene Q without the need for optimization of reaction and cycling conditions. Real-time one-step RT-PCR is carried out, which means that RNA is used as template in a reaction where reverse transcription and PCR take place sequentially in the same reaction vessel. Since it is not necessary to transfer the finished RT reaction to another tube for PCR, the real-time RT-PCR procedure is streamlined, making high-throughput analysis possible. The fluorescent dye SYBR Green I in the master mix enables the analysis of many different targets without having to synthesize target-specific labeled probes. Highly specific amplification is assured through a balanced combination of K+ and NH4+ ions, which promote specific primer annealing, enabling high PCR specificity and sensitivity (see figure "Specific primer annealing"). Fast cycling without compromising performance is achieved using Q-Bond, a novel PCR additive that considerably shortens cycler run times (see figure "Fast primer annealing"). Components of 2x Rotor-Gene SYBR Green RT-PCR Kit* ComponentFeatures Benefits HotStarTaq Plus DNA Polymerase 5 min activation at 95ºC Set up of qPCR reactions at room temperature Rotor-Gene SYBR Green RT-PCR Buffer Balanced combination of NH4+ and K+ ions Specific primer annealing ensures reliable qPCR results Unique Q-Bond additive Faster PCR run times enable faster results and more reactions per day SYBR Green I dye Yields a strong fluorescent signal upon binding double-stranded DNA Highly sensitive quantification Rotor-Gene RT Mix   Special blend of reverse transcriptases with a high affinity for RNA RNA can be transcribed in just 10 minutes, even through complex secondary structures* Also contains dNTP mix (dATP, dCTP, dGTP, dTTP).Procedure A ready-to-use master mix eliminates the need for optimization of reaction and cycling conditions. Simply add template RNA, primers, and the supplied reverse transcriptase mix to the master mix and program the cycler. Instructions are provided in the detailed handbook supplied with the kit. For gene expression analysis using real-time one-step RT-PCR, the combination of the Rotor-Gene SYBR Green RT-PCR Kit with QuantiTect Primer Assays and the Rotor-Gene Q provides a complete, ready-to-run solution. QuantiTect Primer Assays are bioinformatically validated primer sets for any gene from human, mouse, rat, and many other species. Assays can be easily ordered online at the GeneGlobe Web portal.Applications The Rotor-Gene SYBR Green RT-PCR Kit provides rapid real-time quantification of RNA targets on the Rotor-Gene Q. The kits are also compatible with the Rotor-Gene 3000 and the Rotor-Gene 6000. SpecificationsFeaturesSpecifications ApplicationsReal-time quantification of RNA targets DescriptionFor ultrafast quantitative real-time one-step RT-PCR using SYBR Green I Reaction typeReal-time one-step RT-PCR Real-time or endpointReal-time Sample/target typeRNA Single or multiplexSingle SYBR Green I or sequence-specific probesSYBR Green I Thermal cyclerRotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000 With or without ROXWithout ROX dyeProduct ResourcesYou are not authorized to download the resourceBrochures & Guides (1) Sort optionsSort alphabetically (EN) - Rotor-Gene Q - Pure DetectionNow with even more applications!Show detailsShow allShow lessFAQs (12) Sort optionsSort alphabetically What is a QuantiTect Primer Assay?FAQ ID -1141ViewHow do you achieve fast cycling, yet still deliver the same performance in PCR as that achieved with standard cycling?FAQ ID -1430ViewDo you have a protocol for Rotor-Gene software setup for the Rotor-Gene SYBR Green PCR and RT-PCR Kits?FAQ ID -2104ViewAre Rotor-Gene Kits compatible with reaction setup using the QIAgility instrument?FAQ ID -2116ViewDo the master mixes in Rotor-Gene Kits contain dUTP to allow UNG pretreatment?FAQ ID -2117ViewWhy is the initial activation step different for Rotor-Gene Probe, SYBR Green and Multiplex Kits?FAQ ID -2118ViewWhat are the main differences between Rotor-Gene and QuantiTect or QuantiFast PCR Kits?FAQ ID -2119ViewWill Rotor-Gene Kits also work on the Rotor-Gene 6000 and 3000 cyclers?FAQ ID -2121ViewWhy is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits?FAQ ID -2122ViewDo you have any information or guidelines regarding the choice of reference genes for real-time PCR?FAQ ID -2371ViewHow do I create a workspace that is free of DNA contamination, prior to carrying out a qPCR experiment? FAQ ID -2654ViewHow important is the RNA purification process, for obtaining reliable qRT-PCR results? FAQ ID -2655ViewShow allShow lessKit Handbooks (2) Sort optionsSort alphabetically (EN) - Rotor-Gene SYBR Green HandbookFor fast real-time PCR, two-step RT-PCR, and one-step RT-PCR using SYBR Green I on Rotor-Gene cyclersShow details(EN) - QuantiTect Primer Assay HandbookFor genomewide, ready-to-use real-time RT-PCR assays using SYBR Green detectionShow detailsShow allShow lessSupplementary Protocols (1) Sort optionsSort alphabetically Rotor-Gene® software setup for the Rotor-Gene SYBR® Green RT-PCR Kit - (EN)Show detailsShow allShow lessSafety Data Sheets (1) Sort optionsSort alphabetically MSDS Rotor-Gene SYBR Green RT-PCR Kit (400)Show allShow lessfragment fix placeholderCustomers who bought these products also boughtCat No./ID:204574Rotor-Gene Probe RT-PCR Kit (400)For 400 x 25 µl reactions: 3 x 1.7 ml 2x Rotor-Gene Probe RT-PCR Master Mix, 100 µl Rotor-Gene RT Mix, 2 x 2 ml RNase-Free Water$707.00Add To CartCat No./ID:9019725Rotor-Disc Heat Sealer 230VHeat sealing instrument for use with Rotor-Discs; requires Rotor-Disc 72 or 100 Loading Block$5,719.00Add To CartCat No./ID:9018897Rotor-Disc Pipetting AidAid for marking well during manual reaction setup on a Rotor-Disc Loading Block$147.00Add To CartCat No./ID:9018908Rotor HolderMetal free-standing holder for assembling tubes and Rotor-Discs into rotors$654.00Add To CartCat No./ID:204074Rotor-Gene SYBR Green PCR Kit (400)For 400 x 25 µl reactions: 3 x 1.7 ml 2x Rotor-Gene SYBR Green PCR Master Mix, 2 x 2 ml RNase-Free Water$472.00Add To CartCat No./ID:204974Rotor-Gene Multiplex RT-PCR Kit (400)For 400 x 25 µl reactions: 3 x 1.7 ml 2x Rotor-Gene Multiplex RT-PCR Master Mix, 100 µl Rotor-Gene RT Mix, 2x2 ml RNase-Free Water$1,038.00Add To CartCat No./ID:333414QIAseq DNA QuantiMIZE Assay KitAssay 100 and Assay 200 for 400 x 25µl reactions (400µl) each; QuantiMIZE Control gDNA (50µl); RNase- and DNase-free water (1 ml x 4); GeneRead qPCR SYBR Green Mastermix (1.35 ml x 4)Select TargetsCat No./ID:9018905Loading Block 96 x 0.2 ml TubesAluminum block for manual reaction set-up in a standard 8 x 12 array using 96 x 0.2 ml tubes$355.00Add To CartCat No./ID:9018896Rotor-Disc 100 Locking RingFor locking a Rotor-Disc 100 in the Rotor-Disc 100 Rotor$343.00Add To CartCat No./ID:9018895Rotor-Disc 100 RotorFor holding Rotor-Disc 100 discs with reaction volumes of 15–25 µl in the Rotor-Gene Q; requires Rotor-Disc 100 Locking Ring$570.00Add To CartCat No./ID:981103Strip Tubes and Caps, 0.1 ml (250)250 strips of 4 tubes and caps for 1000 reactions of 10–50 µl$258.00Add To CartCat No./ID:47154AllPrep Fungal DNA/RNA/Protein Kit (50)For the extraction ofprotein and nucleic acids from fungal cell cultures$550.00Add To CartCat No./ID:931447InvestigatorSTAR Lyse&PrepKit (400)For 400 preps of 300 µl each from casework and reference samples: Buffer ATL, Buffer QSL3, Buffer QSW1, Buffer QSW2, Bead Suspension G, Buffer ATE, Proteinase K, Carrier RNA, Q-Card, Handbook$1,880.00Add To CartCat No./ID:206045Type-it Fast SNP Probe PCR Kit (800)For 800 x 25 μl reactions: 6 x 1.7 ml of 2x Type-it Fast SNP Probe PCR Master Mix, 5x Q-Solution, RNase-Free Water$786.00Add To CartCat No./ID:981311Rotor-Disc 100 (30)30 individually wrapped discs for 3000 reactions of 15–25 µl$386.00Add To CartCat No./ID:981400Rotor-Disc OTV KitKit for optical temperature verification of Rotor-Gene systems, includes a Rotor-Disc 72 preloaded with thermochromatic liquid crystals, fluorescent inserts, CD with calibration files; requires Rotor-Disc 72 Rotor and Locking Ring or Rotor-Disc 72 Starter Kit$568.00Add To Cart