PCR Enzymes & KitsEnd-Point PCR17HiFidelity, Long-Range & Other PCR6One-Step RT-PCR2Reverse Transcription & cDNA Synthesis2HotStarTaq Plus DNA PolymerasePrint For fast and highly specific amplification in all applicationsHigh PCR specificity with minimal optimizationFast 5-minute enzyme activation timeReady-to-load PCR buffer for faster and easier handlingThe polymerase combines the high specificity, sensitivity, and minimal optimization of HotStarTaq DNA Polymerase, with a fast 5-minute activation time. PCRcan be set up at room temperature and reactions can be directly loaded onto a gel, due to novel CoralLoad PCR Buffer, which contains two gel-tracking dyes. The standard QIAGEN PCR Buffer is also included for greater convenience. In addition, Q-Solution, a novel additive that enables efficient amplification of "difficult" (e.g., GC rich) templates, is also provided. The unique kit components and optimized protocols streamline the PCR procedure.Buy ProductsProduct DetailsSpecificationsProduct ResourcesBuy ProductsCat No./ID:203603HotStarTaq Plus DNA Polymerase (250)$167.00Order Product250 units HotStarTaq Plus DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2Cat No./ID:203605HotStarTaq Plus DNA Polymerase (1000)$593.00Order Product1000 units HotStarTaq Plus DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2Cat No./ID:203607HotStarTaq Plus DNA Polymerase (5000)$2,658.00Order Product5000 units HotStarTaq Plus DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2Cat No./ID:203609HotStarTaq Plus DNA Polymerase (25000)$11,983.00Order Product25,000 units HotStarTaq Plus DNA Polymerase, 10x PCR Buffer, 10x CoralLoad PCR Buffer, 5x Q-Solution, 25 mM MgCl2The HotStarTaq Plus DNA Polymerase is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.Product Details Amplification of difficult templates.Highly sensitive single-cell PCR.Higher specificity with different primer–template systems.HotStarTaq Plus procedure.The HotStarTaq Plus procedure is fast and easy for maximum convenience.Highest specificity.PCR was performed with HotStarTaq Plus DNA Polymerase, HotStarTaq DNA Polymerase, and Taq DNA Polymerase from QIAGEN, and 3 hot-start PCR enzymes from the indicated suppliers. Parallel reactions were performed following the suppliers" recommendations, using 50 ng human genomic DNA. A 1.5 kb fragment of the human CFTR gene was amplified in 35 PCR cycles. M: markers.Effect of hot start on RT-PCR performance.Increased specificity of primer annealing.CoralLoad PCR Buffer.PerformanceEach lot of HotStarTaq Plus DNA Polymerase is subjected to a comprehensive range of quality control tests, including a stringent PCR specificity and reproducibility assay in which low-copy targets are amplified. HotStarTaq Plus DNA Polymerase outperformed kits tested from other suppliers and ensures high specificity and superior performance in hot-start PCR (see figures "Highest specificity" and "Higher specificity with different primer–template systems", and table). The innovative PCR buffer provided with the kit ensures specificity over a wide range of PCR conditions, minimizing the need for optimization. Suboptimal PCR can be improved with Q-Solution, also provided with the kit (see figure "Amplification of difficult templates"). Together, these components ensure specific amplification in a range of applications (see figure "Effect of hot start on RT-PCR performance" and "Highly sensitive single-cell PCR"). Comparison of hot-start methods HotStarTaq Plus DNA Polymerase HotStarTaq DNA Polymerase Hot-start enzyme from Supplier AII Supplier R Supplier I (antibody-mediated) Manual Wax barrier Specific amplification ++ ++ + ++ + +/– +/– Minimal PCR optimization ++ ++ +/– +/– +/– – – Easy to use +++ ++ ++ + + – – Speed of activation ++ + ++ ++ ++ – – HotStarTaq Plus DNA Polymerase specifications Concentration: 5 units/µlRecombinant enzyme: YesSubstrate analogs: dNTP, ddNTP, dUTP, biotin-11-dUTP, DIG-11-dUTP, fluorescent-dNTP/ddNTPExtension rate: 2–4 kb/min at 72°CHalf-life: 10 min at 97°C ; 60 min at 94°CAmplification efficiency: ≥105 fold5"–>3" exonuclease activity: YesExtra A addition: Yes3"–>5" exonuclease activity: NoContaminating nucleases: NoContaminating RNases: NoContaminating proteases: NoSelf-priming activity: NoPrincipleHotStarTaq Plus DNA Polymerase provides the unrivaled performance of HotStarTaq DNA Polymerase with a shortened activation time of just 5 minutes. HotStarTaq Plus DNA Polymerase, a modified form of QIAGEN Taq DNA Polymerase, is supplied in an inactive state that has no polymerase activity at ambient temperatures. This prevents extension of nonspecifically annealed primers and primer dimers formed at low temperatures during PCR setup and the initial PCR cycle (see figures "Highest specificity" and "Higher specificity with different primer-template systems"). HotStarTaq Plus DNA Polymerase is activated by a short 5-minute incubation at 95°C which can be easily incorporated into any existing thermal-cycler program. QIAGEN PCR Buffer QIAGEN PCR Buffer maintains specific amplification in every cycle of PCR by promoting a high ratio of specific-to-nonspecific primer binding during the annealing step in each PCR cycle (see figure "Increased specificity ofprimer annealing"). Owing to a uniquely balanced combination of KCl and (NH4)2SO4, the buffer provides stringent primer-annealing conditions over a wider range of annealing temperatures and Mg2+ concentrations than conventional PCR buffers. Optimization of PCR by varying the annealing temperature or the Mg2+ concentration is therefore often minimal or not required. CoralLoad PCR Buffer HotStarTaq Plus DNA Polymerase is supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity and minimal reaction optimization as the conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes — an orange dye and a red dye — that facilitate estimation of DNA migration distance and optimization of agarose gel run time (see figure "CoralLoad PCR Buffer").The buffer ensures improved pipetting visibility and enables direct loading of PCR products onto a gel, for enhanced convenience. Q-Solution Q-Solution, an innovative PCR additive that facilitates amplification of difficult templates by modifying the melting behavior of DNA, is also provided with HotStarTaq DNA Polymerase. This unique reagent improves suboptimal PCR caused by templates that have a high degree of secondary structure or GC-rich templates (see figure "Amplification of difficult templates"). Unlike other commonly used PCR additives such as DMSO, Q-Solution is used at just one working concentration, is nontoxic, and PCR purity is guaranteed. Adding Q-Solution to the PCR does not compromise PCR fidelity. ProcedureHotStarTaq Plus DNA Polymerase is supplied with a streamlined, optimized protocol for fast and easy PCR setup. HotStarTaq Plus DNA Polymerase is activated by a 5-minute, 95°C incubation step, which can easily be incorporated into existing thermal cycling programs.Reactions can be set up at room temperature, ensuring greater convenience and ease of use (see figure "HotStarTaq Plus procedure"). CoralLoad PCR Buffer, alsoprovided with the kit, ensures improved pipetting visibility and enables direct loading of PCR products onto a gel for enhanced convenience.Applications HotStarTaq Plus DNA Polymerase is highly suitable for a wide variety of applications, including challenging applications such as amplification of: Complex genomic templates Complex cDNA templates (e.g., RT-PCR) Very low-copy targets (e.g., single-cell PCR) Reactions with multiple primer pairs SpecificationsFeaturesSpecifications ApplicationsPCR, RT-PCR, Complex genomic templates, very low-copy targets Enzyme activity5" -> 3" exonuclease activity MastermixNo Reaction typePCR amplification Real-time or endpointEndpoint Sample/target typeGenomic DNA and cDNA Single or multiplexSingle With/without hotstartWith hotstartProduct ResourcesYou are not authorized to download the resourceBrochures & Guides (2) Sort optionsSort alphabetically Analyzing Genetic Differences - (EN)Second edition — innovative toolsShow details(EN) - Maximizing PCR and RT-PCR success — Third EditionAddressing critical factors and new solutionsShow detailsShow allShow less (1) Sort optionsSort alphabetically Product Profile - HotStarTaq® Plus DNA Polymerase and Master MixShow detailsShow allShow lessFAQs (9) Sort optionsSort alphabetically What kind of PCR products can be cloned with the QIAGEN PCR Cloning Kit?FAQ ID -165ViewWhat is the largest PCR amplicon that can be amplified with the HotStar HiFidelity Polymerase Kit?FAQ ID -1047ViewDoes an activation time of 15 minutes influence the performance of the HotStarTaq Plus DNA Polymerase?FAQ ID -1050ViewAre the CoralLoad dyes in the HotStarTaq Plus PCR Buffer visible when loading small amounts of PCR product onto a gel?FAQ ID -1051ViewDo I have to use CoralLoad Gel loading dye when using your HotStarTaq Plus DNA Polymerase?FAQ ID -1052ViewWhy is the activation time for HotStarTaq Plus Polymerase in the QuantiFast SYBR Green Kits different from that for QuantiFast Probe Kits?FAQ ID -1449ViewHow comparable is CoralLoad gel loading dye contained in various QIAGEN PCR Kits to Sigma Red?FAQ ID -1644ViewIs Q-Solution required for PCR with QIAGEN"s PCR kits?FAQ ID -380ViewDo you have a protocol for polyacrylamide gel analysis of oligonucleotides?FAQ ID -961ViewShow allShow lessKit Handbooks (1) Sort optionsSort alphabetically HotStarTaq Plus PCR Handbook - (EN)For highly specific hot-start PCR without optimization Show detailsShow allShow lessTechnical Information (1) Sort optionsSort alphabetically (EN) - Maximizing end-point PCR success with QIAGEN"s automatable PCR solutionsShow detailsShow allShow lessQuick-Start Protocols (1) Sort optionsSort alphabetically HotStarTaq Plus DNA Polymerase (EN)Show detailsShow allShow lessSafety Data Sheets (5) Sort optionsSort alphabetically MSDS HotStarTaq Plus DNA Polymerase (250)MSDS HotStarTaq Plus DNA Polymerase (1000)MSDS HotStarTaq Plus DNA Polymerase (5000)MSDS HotStarTaq Plus DNA Polymerase (25000)CofAShow allShow less References 0 fragment fix placeholderCustomers who bought these products also boughtCat No./ID:201901dNTP Mix, PCR Grade (800 µl)Mix containing 10 mM each of dATP, dCTP, dGTP, and dTTP (4 x 200 µl)$180.00Add To CartCat No./ID:990399Spin Column Adapter Rings (25)Spin column adapter rings for use with spin columns that have screw caps; not required for all protocols$149.00Add To CartCat No./ID:12281QIAfilter Plasmid Mega Kit (5)5 QIAGEN-tip 2500, Reagents, Buffers, 5 QIAfilter Mega-Giga Cartridges$391.00Add To CartCat No./ID:185446GeneRead QIAact Panel Cleanup Kit (48)Reagents for automated DNA cleanup on the QIAcubeof 48 libraries prepared using the GeneRead QIAact Lung DNA Panel Kit.$80.70Add To CartCat No./ID:201900dNTP Mix, PCR Grade (200 µl)Mix containing 10 mM each of dATP, dCTP, dGTP, and dTTP (1 x 200 µl)$51.70Add To CartCat No./ID:210215QIAGEN OneStep RT-PCR Kit (1000)For 1000 x 50 µl reactions (available in a single tube): QIAGEN OneStep RT-PCR Enzyme Mix (2 x 1 ml), 5x QIAGEN OneStep RT-PCR Buffer (1 x 10 ml), dNTP Mix (2 x 1 ml, 10 mM each), 5x Q-Solution (1 x 10 ml), RNase-Free Water (1 x 40 ml)$5,355.00Add To CartCat No./ID:180412QIAseq 1-Step Amplicon Library Kit (12)For 12 reactions: 1-Step Amplicon Enzyme Mix, 4x 1-Step Amplicon Buffer, Primer Mix Illumina Library Amp, HiFi PCR Master Mix, RNase-Free Water. 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