Protein PurificationCrystallization9Fractionation & Depletion10Tagged Protein Expression, Purification, Detection37Penta·His Alexa Fluor 647 ConjugatePrint For sensitive, direct immunofluorescent detection of His-tagged proteins, with an emission peak at 647nmHighly specific and sensitive detection of His-tagged proteinsDirect detection without the need for a secondary antibodyBroad instrument compatibility and high photostability Penta·His Alexa Fluor 647 Conjugate is for sensitive, direct immunofluorescent detection of recombinant proteins carrying His tags (consisting of at least five consecutive histidine residues) at 647 nm. Penta·His Alexa Fluor Conjugates are used for applications including immunocytochemistry, protein localization and targeting studies, and fluorescence-activated cell sorting (FACS). Buy ProductsProduct DetailsSpecificationsProduct ResourcesBuy ProductsCat No./ID:35370Penta·His Alexa Fluor 647 Conjugate$255.00Order Product125 μl Penta·His Alexa Fluor 647 Conjugate, 200 μg/mlThe Penta·His Alexa Fluor 647 Conjugate is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.Product DetailsPerformance Performance of Penta·His Alexa Fluor 647 ConjugatePropertyValueDetection:Five consecutive histidine residuesAntibody isotype:Mouse IgG1Cross-reactivity:Negligible with crude E. coli, yeast, mammalian, or insect cell lysatesForm:Stabilized stock solutionWorking solution:Dependent on applicationPrinciple Penta·His Antibodies are available as Alexa Fluor 488, 532, 555, and 647 conjugates, giving a range of highly specific reagents whose emission wavelengths cover a wide portion of the visible spectrum. ProcedureThe QIAexpress, Detection and Assay Handbook, supplied with all QIAexpress detection and assay products, contains detailed example protocols and guidelines for designing and performing assays.Applications The Penta·His Alexa Fluor 647 Conjugate can be used in immunofluorescence procedures such as flow cytometry, confocal laser microscopy, immunocytochemistry, immunohistochemistry, protein localization, and targeting studies SpecificationsFeaturesSpecifications DetectionDirect Epitope detectedHHHHH TagHis tagProduct ResourcesYou are not authorized to download the resourceKit Handbooks (1) Sort optionsSort alphabetically QIAexpress Detection and Assay Handbook - (EN)For Anti·His Antibodies,Anti·His HRP Conjugates, Penta·His™ Alexa Fluor® Conjugates, Penta·His Biotin Conjugate, Ni-NTA Conjugates, Tag·100™ Antibody, Streptavidin–R-PE, 6xHis Protein Ladder, Ni-NTA HisSorb™ Strips and PlatesShow detailsShow allShow lessSafety Data Sheets (1) Sort optionsSort alphabetically MSDS Penta·His Alexa Fluor 647 ConjugateShow allShow lessfragment fix placeholderCustomers who bought these products also boughtCat No./ID:35310Penta·His Alexa Fluor 488 Conjugate125 μl Penta·His Alexa Fluor 488 Conjugate, 200 μg/ml$255.00Add To CartCat No./ID:34440Penta·His Biotin Conjugate125 μl Penta·His Biotin Conjugate, 200 μg/ml$407.00Add To CartCat No./ID:34650RGS·His Antibody, BSA-free (100 μg)100 μg mouse anti·RGS(H)4 (lyophilized, BSA-free, for 1000ml working solution)$742.00Add To CartCat No./ID:27500-4-EP-BPPowerBead Plates, Glass (4)MagAttract PowerMicrobiome DNA/RNA EP Kit Components$311.00Add To CartCat No./ID:339459miRCURY LNA miRNA ISH Buffer and ControlsScramble miRNA Negative Control Probe, U6 snRNA Positive Control Probe, 2x Formamide-free miRNA ISH buffer, Proteinase K; for 400 slides$1,490.00Add To CartCat No./ID:30622Ni-NTA Superflow Columns (12 x 1.5 ml)For 12 6xHis-tagged protein preps: 12 polypropylene columns containing 1.5 ml Ni-NTA Superflow$428.00Add To CartCat No./ID:3693515 ml/50 ml Tube MagnetMagnet for separating magnetic particles in 5 x 15 ml and 3 x 50 ml tubes$858.00Add To CartCat No./ID:30310NTA Agarose (25 ml)25 ml uncharged resin (max. pressure 2.8 psi)$329.00Add To CartCat No./ID:19591Caps for Elution Microtubes (50x8)Caps for Elution Microtubes (50x8)$13.50Add To CartCat No./ID:3691212-Tube MagnetMagnet for separating magnetic beads in 12 x 1.5 ml or 2 ml tubes$399.00Add To CartCat No./ID:84020Vacuum Pump (230 V, 50 Hz)Universal vacuum pump (capacity 34 L/min, 8 mbar vacuum abs.)$2,658.00Add To CartCat No./ID:12877-50DNeasy PowerClean Cleanup Kit (50)For secondary DNA clean-up and removal of inhibitors from heparin$228.00Add To CartCat No./ID:158389Gentra Puregene Blood Kit (1000 ml)For 1000 ml blood: RBC Lysis Solution and Reagents$1,316.00Add To CartCat No./ID:301005Attractene Transfection Reagent (1 ml)Attractene Transfection Reagent for up to 660 transfections in 24-well plates$422.00Add To CartCat No./ID:180514GeneRead Size Selection Kit (50)For 50 reactions: Spin columns and buffers$142.00Add To CartCat No./ID:931236QIAsymphony DNA Mini Kit (192)For 192 preps of 200 µl each: includes 2 reagent cartridges and enzyme racks and accessoriesAdd To CartCat No./ID:19571AirPore Tape Sheets (50)Microporous tape sheets for covering 96-well blocks: 50 sheets per pack$111.00Add To CartCat No./ID:1031576Buffer TCL (125 ml)125 ml of lysis buffer for TurboCapture mRNA Kits$239.00Add To Cart