Protein PurificationCrystallization9Fractionation & Depletion10Tagged Protein Expression, Purification, Detection37Ni-NTA Magnetic Agarose BeadsPrint For high-throughput, micro-scale purification of His-tagged proteins and versatile magnetocapture assays using His tags Directed presentation for enhanced signal-to-noise and reproducibility Wide range of binding capacities by varying the number of beadsEffective screening procedures even with crude cell lysates Ideal for study of biomolecular interactions Options for full automation Ni-NTA Magnetic Agarose Beads are magnetic particles coated with Ni-NTA Agarose affinity purification matrix. They are used for immobilizing and purifying recombinant proteins carrying a His tag. Histidine residues in the His tag bind to the vacant positions in the coordination sphere of the immobilized nickel ions with high specificity and affinity. Once proteins are bound, the beads can be precipitated using a magnet, washed, and proteins eluted in small volumes of buffer under native or denaturing conditions. Buy ProductsProduct DetailsSpecificationsProduct ResourcesBuy ProductsCat No./ID:36111Ni-NTA Magnetic Agarose Beads (2 x 1 ml)$249.00Order Product2 x 1 ml nickel-charged magnetic agarose beads (5%suspension)Cat No./ID:36113Ni-NTA Magnetic Agarose Beads (6 x 1 ml)$703.00Order Product6 x 1 ml nickel-charged magnetic agarose beads (5%suspension)Ni-NTA Magnetic Agarose Beads are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.Product Details Micrograph of Ni-NTA Magnetic Agarose Beads.Magnetic particles are seen inside the beads.Micro-scale protein purification.Principle The QIAexpress Ni-NTA Protein Purification System, including the Ni-NTA Magnetic Agarose Beads (see figure Micrograph of Ni-NTA Magnetic Agarose Beads) is based on the remarkable selectivity of patented Ni-NTA (nickel-nitrilotriacetic acid) resin for proteins containing an affinity tag of six or more histidine residues — the His tag. This technology allows one-step purification of almost any His-tagged protein from any expression system under native or denaturing conditions (see figure Protein purification with the Ni-NTA protein purification system). NTA, which has four chelation sites for nickel ions, binds nickel more tightly than metal-chelating purification systems that only have three sites available for interaction with metal ions. The extra chelation site prevents nickel ion leaching and results in a greater binding capacity and protein preparations with higher purity than those obtained using other metal-chelating purification systems. The QIAexpress system can be used to purify His-tagged proteins from any expression system including baculovirus, mammalian cells, yeast, and bacteria. Procedure The purification of His-tagged proteins consists of 4 stages: cell lysis, binding, washing, and elution (see figure Micro-scale protein purification). Purification of recombinant proteins using the QIAexpress system does not depend on the 3-dimensional structure of the protein or His tag. This allows one-step protein purification under either native or denaturing conditions, from dilute solutions and crude lysates. Strong denaturants and detergents can be used for efficient solubilization and purification of receptors, membrane proteins, and proteins that form inclusion bodies. Reagents that allow efficient removal of nonspecifically binding contaminants can be included in wash buffers (see table). Purified proteins are eluted under mild conditions by adding 100–250 mM imidazole as competitor or by a reduction in pH.   Reagents Compatible with the His/Ni-NTA Interaction DenaturantsDetergentsReducing agents Others Salts For long-term storage 6 M Gu·HCl 2% Triton X-100 20 mM β-ME 50% glycerol 4 M MgCl2 Up to 30% ethanol 8 M urea 2% Tween 20 10 mM DTT 20% ethanol 5 mM CaCl2 or 100 mM NaOH  1% CHAPS 20 mM TCEP20 mM imidazole 2 M NaCl       Applications The QIAexpress Ni-NTA Protein Purification System, including the Ni-NTA Magnetic Agarose Beads, provides reliable, one-step purification of proteins suitable for any application, including: Structural and functional investigations Crystallization for determination of three-dimensional structure Assays involving protein–protein and protein–DNA interactions Immunization to produce antibodies SpecificationsFeaturesSpecifications ApplicationsProteomics Bead size20–70 µm Binding capacityUp to 2 mg/ml suspension (5%) ProcessingAutomated/manual ScaleMicro scale Special featureHigh throughput (96-well) Start materialCell lysate Support/matrixMagnetic agarose beads Tag6xHis tag YieldProduct ResourcesYou are not authorized to download the resourceFAQs (6) Sort optionsSort alphabetically How can I remove imidazole from a protein sample?FAQ ID -91ViewHow can I eliminate contaminating protein in my Ni-NTA 6xHis-tag protein purification?FAQ ID -102ViewHow can I purify very small amounts of 6xHis-tagged protein using Ni-NTA technology?FAQ ID -134ViewWhat are the features and benefits of the QIAexpress 6xHis Tag System?FAQ ID -193ViewDo you have a protocol for the purification of 6xHis-tagged proteins using BioSprint?FAQ ID -1167ViewCan Ni-NTA resins be used to purify protein with an internal His-tag?FAQ ID -496ViewShow allShow lessKit Handbooks (2) Sort optionsSort alphabetically (EN) - Ni-NTA Magnetic Agarose BeadsFor Manual and automated Assays using 6xHis-tagged proteins Purification of 6xHis-tagged proteinsShow details(EN) - Important Note for Ni-NTA UsersShow detailsShow allShow lessTechnical Information (2) Sort optionsSort alphabetically Critical factors for successful protein crystallization - (EN)Show detailsReliable purification of GST-, His-, and Strep-tagged proteins - (EN)Show detailsShow allShow lessScientific Posters (1) Sort optionsSort alphabetically (EN) - Process Development: Scaling Up Human IL-1b Production, Tag Removal, and X-Ray CrystallographyShow detailsShow allShow lessSupplementary Protocols (1) Sort optionsSort alphabetically Purification of 6xHis-tagged proteins using the BioSprint 96 workstation - (EN)Show detailsShow allShow lessQuick-Start Protocols (1) Sort optionsSort alphabetically Purification of 6xHis-tagged Proteins in Single Reaction Tubes with Ni-NTA Magnetic Agarose Beads (EN)Show detailsShow allShow lessSafety Data Sheets (3) Sort optionsSort alphabetically MSDS Ni-NTA Magnetic Agarose Beads (2 x 1 ml)MSDS Ni-NTA Magnetic Agarose Beads (6 x 1 ml)CofAShow allShow less References 0 fragment fix placeholderCustomers who bought these products also boughtCat No./ID:32915N-Terminus pQE Vector Set25 µg each: pQE-9, pQE-30, pQE-31, pQE-32, pQE-40$718.00Add To CartCat No./ID:36910Single-Tube MagnetMagnet for separating magnetic particles in a 1.5 ml or 2 ml tube$221.00Add To CartCat No./ID:34924Polypropylene Columns (1 ml)50/pack, 1 ml capacity$147.00Add To CartCat No./ID:27000-4-KF-3ClearMag Binding Solution (200 ml)MagAttract PowerSoil DNA KF Kit Components (Optimized for KingFisher).$193.00Add To CartCat No./ID:19015QIArack1 rack for 12x QIAGEN-tip 20, 8x QIAGEN-tip 100, 6x QIAGEN-tip 500 or HiSpeed Midi Tips, 4x QIAGEN-tip 2500 or HiSpeed Maxi Tips, or 10x QIAfilter Midi or Maxi Cartridges$115.00Add To CartCat No./ID:1069275Buffer ACB (300 ml)Buffer ACB (300 ml)$169.00Add To CartCat No./ID:301005Attractene Transfection Reagent (1 ml)Attractene Transfection Reagent for up to 660 transfections in 24-well plates$422.00Add To CartCat No./ID:37901Qproteome Mammalian Protein Prep KitFor approximately 100 protein preparations from cultured mammalian cells: Buffer, Reagents, Protease Inhibitor Solution, Benzonase$234.00Add To CartCat No./ID:204074Rotor-Gene SYBR Green PCR Kit (400)For 400 x 25 µl reactions: 3 x 1.7 ml 2x Rotor-Gene SYBR Green PCR Master Mix, 2 x 2 ml RNase-Free Water$471.00Add To CartCat No./ID:30600Ni-NTA Fast Start Kit (6)For purification and detection of six 6xHis-tagged protein preps: 6 x Fast Start Columns, Penta·His Antibody, Buffers and Reagents$367.00Add To CartCat No./ID:338125384EZLoad Covers (4)384EZLoad™ Covers$24.90Add To CartCat No./ID:37623Qproteome FFPE Tissue Kit (20)For 20 protein preparations from formalin-fixed, paraffin-embedded tissue samples: Extraction Buffer, Collection Tubes, Collection Tube Sealing Clips$441.00Add To CartCat No./ID:31314Ni-NTA Spin Kit (50)50 Ni-NTA Spin Columns, Reagents, Buffers, Collection Tubes, 1 μg Control Expression Plasmid$554.00Add To CartCat No./ID:763134PAXgene Blood miRNA Kit (50)For 50 RNA preps: PAXgene Spin Columns, PAXgene Shredder Spin Columns, Processing Tubes, Microcentrifuge Tubes, RNase-Free DNase, RNase-Free Reagents and Buffers; to be used in conjunction with PAXgene Blood RNA Tubes$717.00Add To CartCat No./ID:12867-25RNeasy PowerSoil DNA Elution Kit (25)For co-isolation of DNA and RNA from soil using the RNA PowerSoil Total RNA Isolation Kit$137.00Add To CartCat No./ID:20912Buffer QX1 (500 ml)500 ml Solubilization and Binding Buffer (with pH indicator)$107.00Add To CartCat No./ID:19606MagAttract Magnetic RackMagnetic rack for convenient parallel processing of up to 12 samples$988.00Add To CartCat No./ID:12843CompactPrep Plasmid Midi Kit (25)25 CompactPrep Midi Columns, Extender tubes, Reagents, Buffers, LyseBlue$290.00Add To CartCat No./ID:158928Lytic Enzyme Solution (650 µl)650 µl Lytic Enzyme Solution$84.80Add To CartCat No./ID:69990Stainless Steel Beads, 7 mm (200)200 stainless steel beads (7 mm diameter), suitable for use with TissueLyser systems$268.00Add To Cart