Diagnostics & Clinical ResearchInfectious Disease6Oncology4Sample Processing118Sexual & Reproductive Health0Solutions for Laboratory-Developed Tests15TB Management2QuantiFERON SARS-CoV-2 RUOPrint Maximize insights from SARS-CoV-2 T cell immune responseBased on world-leading QuantiFERON IGRA technologyCustomizable solution to fit your research needsUnlock immune response potential for better research insightsAs T cells are becoming increasingly recognized for their role in SARS-CoV-2 infection and immunity, the exploration of immune response markers in COVID-19 infections is becoming the next tool for researching potential improvements in patient management and vaccination guidance (10–12).QIAGEN is widely committed in the fight against COVID-19 and you can now count on the world-leading QuantiFERON IGRA technology to support your research toward fighting this pandemic.The QuantiFERON SARS-CoV-2 Blood Collection Tubes and QuantiFERON ELISA are for Research Use Only and not for diagnostic use.Buy ProductsProduct DetailsProduct ResourcesBuy ProductsCat No./ID:626015QuantiFERON Control SetInquireNegative and positive controls; 100 Nil Tube (2 x 50/rack); 100 Mitogen Tube (2 x 50/rack)Cat No./ID:626315QuantiFERON Monitor DirectInquireBlood collection tubes containing specialized stimulants for the detection of both innate and adaptive immune response; 100 QFN Monitor Direct Tube (2 x 50/rack)Cat No./ID:626410QuantiFERON ELISAInquireELISA microplate for the dosage of IFN-γ; Microplate Strips; IFN-gamma Standard, lyophilized; Green Diluent; Conjugate 100x Concentrate, lyophilized; Wash Buffer 20x Concentrate; Enzyme Substrate Solution; Enzyme Stopping Solution (2 x ELISA plates)Cat No./ID:626715QuantiFERON SARS-CoV-2 Starter PackInquireStarter Set (cat. no. 626115) plus Control Set (cat. no. 626015)Starter Set: Spike-specific peptide pool for general assessment of the SARS-CoV-2 immune response; 100 QF-SARS-CoV-2 Ag1 Tube (2 x 50/rack); 100 QF-SARS-CoV-2 Ag2 Tube (2 x 50/rack)Control Set: Negative and positive controls; 100 Nil Tube (2 x 50/rack); 100 Mitogen Tube (2 x 50/rack)The QuantiFERON SARS-CoV-2 RUO is for Research Use Only and not for diagnostic use.Product DetailsPerformanceThe QuantiFERON SARS-CoV-2 Blood Collection Tubes and QuantiFERON ELISA are for Research Use Only and not for diagnostic use. Performances have not been established yet.PrincipleYou decide to work with what matters most for your research: the QuantiFERON SARS-CoV-2 RUO solution is composed of several components that can be ordered separately or combined to provide better research insights on patients’ immune status.The QuantiFERON SARS-CoV-2 RUO solution is an original combination of blood collection tubes containing innovative specific peptides formulated to stimulate lymphocytes in heparinized whole blood involved in cell-mediated immunity. Plasma from the stimulated samples can then be used for detection of IFN-γ using a simple ELISA.ProcedureThe QuantiFERON Control Set Blood Collection Tubes (QFN Control BCTs) consist of the QuantiFERON Nil and QuantiFERON Mitogen BCTs. The QFN Control BCT workflow includes blood collection and processing to generate plasma for the detection of IFN-γ. QFN Nil and Mitogen BCTs are intended to be used as negative and positive controls in conjunction with other QuantiFERON Antigen Tubes, when workflow and donor controls are required.The QuantiFERON SARS-CoV-2 Starter Set (QFN SARS-CoV-2) Blood Collection Tube is for Research Use Only and uses a combination of antigens specific to SARS-CoV-2 to stimulate lymphocytes in heparinized whole blood involved in cell-mediated immunity. Plasma from the stimulated samples can be used for detection of cytokines, including IFN-γ. Detection of IFN-γ can be measured using QuantiFERON ELISA. The QFN SARS-CoV-2 Blood Collection Tubes are not for use in diagnostic procedures.The QuantiFERON Monitor Direct (QFM Direct) Blood Collection Tube uses a combination of antigens to stimulate lymphocytes in heparinized whole blood involved in cell-mediated immunity. Plasma from the stimulated samples can be used for detection of IFN-γ, which can be done using QuantiFERON ELISA. The QFM Direct BCT is for Research Use Only and not for diagnostic use.The QuantiFERON ELISA (QFN ELISA) is a microplate coated with specific material for determining the concentration of IFN-γ in a human plasma sample (reported in International Units per ml; IU/ml). Analysis of results is performed using the QuantiFERON Software.ApplicationsNumerous publications and recent research highlight the potential applications of immune response assays in COVID-19 infections, such as, but not limited to:Investigating infection severity in hospitalized COVID-19 patientsInvestigating patient prognosis in ICUInvestigating immunity in at-risk populations to possibly aid in vaccine prioritization or determination of vaccine efficacySoftwareAnalysis software for QuantiFERON SARS-CoV-2 RUO is available for download.ReferencesResearch publications indicating the use of T cell assays in severity assessment and monitoring of individuals1. Rydyznski Moderbacher, C. et al., (2020) Antigens-specific adaptive immunity to SARS-CoV-2 in acute COVID-19 and associations with age and disease severity. Cell 183, 1–17.2. Candon, S. et al., (2020) T cell and antibody responses to SARS-CoV-2: experience from a French transplantation and hemodialysis center during the COVID-19 pandemic. Am J Transplant doi:10.1111/ajt.163483. Han, M. et al., (2020) Assessing SARS-CoV-2 RNA levels and lymphocyte/T cell counts in COVID-19 patients revealed initial immune status as a major determinant of disease severity. Med Microbiol Immunol 209, 657–668.4. Iqbal, H. (2020) The importance of cell-mediated immunity in COVID-19 – An opinion. Medical Hypotheses 143, 110152.5. Liu, B. et al., (2020) Reduced numbers of T cells and B cells correlates with persistent SARS-CoV-2 presence in non-severe COVID-19 patients. Sci Rep 10, 17718.6. Petrone, L. et al., (2020) A whole blood test to measure SARS-CoV-2 specific response in COVID-19 patients. Clin Microbiol Infect https://doi.org/10.1016/j.cmi.2020.09.0517. Stephens, D.S. and McElrath, M.J. (2020) COVID-19 and the path to immunity. JAMA 324 (13), 1279–1281.8. Zhang, B., Yue, D., Wang, Y., Wang, F., Wu, S. and Hou, H. (2020) The dynamics of immune response in COVID‐19 patients with different illness severity. J Med Virol https://doi.org/10.1002/jmv.26504.9. Poonia, B. and Kottilil, S. (2020) Immune correlates of COVID-19 control. Front Immunol 11, 569611. doi: 10.3389/fimmu.2020.569611Research publications indicating the use of T cell assays in vaccine efficacy, epidemiology survey and protective immunity assessment10. Grifoni, A. et al., (2020) Targets of T cell responses to SARS-CoV-2 coronavirus in humans with COVID-19 disease and unexposed individuals. Cell 181, 1489–1501.11. Murugesan, K. et al., (2020) Interferon-gamma release assay for accurate detection of SARS-CoV-2 T cell response. Clin Infect Dis, ciaa1537, https://doi.org/10.1093/cid/ciaa153712. Sauer, K. and Harris, T. (2020) An Effective COVID-19 vaccine needs to engage T cells. Front Immunol 11, 2371.13 Oladipo, E.K. et al., (2020) Exploration of surface glycoprotein to design multi-epitope vaccine for the prevention of Covid-19. Inform Med Unlocked 21, 100438, https://doi.org/10.1016/j.imu.2020.10043814. Cox, R.J. and Brokstad, K.A. (2020) Not just antibodies: B cells and T cells mediate immunity to COVID-19. Nat Rev Immunol 20, 581–582.15. Mateus, J. et al., (2020) Selective and cross-reactive SARS-CoV-2 T cell epitopes in unexposed humans. Science 370 (6512), 89–94.16. de Vries, R.D. (2020) SARS-CoV-2-specific T-cells in unexposed humans: presence of cross-reactive memory cells does not equal protective immunity. Sig Transduct Target Ther 5, 224.17. Peng, Y. et al., (2020) Broad and strong memory CD4+ and CD8+ T cells induced by SARS-CoV-2 in UK convalescent individuals following COVID-19. Nat Immunol 21, 1336–1345.18. Petrone, L., et al., (2020) A whole blood test to measure SARS-CoV-2 specific response in COVID-19 patients. Clin Microbiol Infect https://doi.org/10.1016/j.cmi.2020.09.051.19. Weiskopf, D. et al., (2020) Phenotype and kinetics of SARS-CoV-2-specific T-cells in COVID-19 patients with acute respiratory distress syndrome. Sci Immunol 5 (48), doi: 10.1126/sciimmunol.abd207120. Sekine, T. et al., (2020) Robust T cell immunity in convalescent individuals with asymptomatic or mild COVID-19. Cell 183 (1), 158–168 E14.Product ResourcesYou are not authorized to download the resourceKit Handbooks (4) Sort optionsSort alphabetically QuantiFERON Control Set Blood Collection Tubes Instructions for Use (Handbook)For Research Use OnlyNot for use in diagnostic proceduresShow detailsQuantiFERON SARS-CoV-2 Starter Set Blood Collection Tubes Instructions for Use (Handbook)For Research Use OnlyNot for use in diagnostic proceduresShow detailsQuantiFERON Monitor Direct Blood Collection Tubes Instructions for Use (Handbook)For Research Use OnlyNot for use in diagnostic proceduresShow detailsQuantiFERON ELISA Instructions for Use (Handbook)For Research Use OnlyNot for use in diagnostic proceduresShow detailsShow allShow lessSoftware User Guides (1) Sort optionsSort alphabetically QuantiFERON R&D Analysis RUO Software User ManualFor installation, setup, and use of QuantiFERON R&D Analysis RUO Software version 5.03.0For Research Use OnlyShow detailsShow allShow lessOperating Software (1) Sort optionsSort alphabetically QuantiFERON R&D Analysis Software (RUO)Version 5.3.0Show detailsShow allShow lessfragment fix placeholder