Sample Collection & StabilizationDNA5RNA19Tissue/FFPE5PAXgene Blood DNA KitPrint For purification of up to 500 µg genomic DNA from whole bloodBlood collection and purification in one systemEasy handlingEnhanced workflow efficiencyThe PAXgene Blood DNA system consists of PAXgene Blood DNA Tubesfor blood collection and stabilization, and the PAXgene Blood DNA Kit for DNA purification in a single-tube procedure. Buy ProductsProduct DetailsSpecificationsProduct ResourcesBuy ProductsCat No./ID:761133PAXgene Blood DNA Kit (25)$366.00Order ProductProcessing tubes and buffers for 25 preparationsFor Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.Product Details High quality and high molecular weight of genomic DNA.Genomic DNA isolated from 8 blood donors using the PAXgene Blood DNA System. [A] Agarose gel analysis; [B] pulsed-field gel electrophoresis for enhanced separation of high-molecular-weight genomic DNA.  M: markers.Efficient multiplex PCR of 3 mitochondrial genes.Multiplex PCR of fragments from the mitochondrial genes tRNAlys/ATPase (0.92 kb), tRNAleu(UUR) (0.63 kb), and ND4 (0.29 kb), using 250 ng DNA from 8 donors as starting material.PAXgene Blood DNA procedure.Blood samples (8.5 ml) are collected in PAXgene Blood DNA Tubes, where they may be stored or transported. For DNA isolation, the blood is transferred to processing tubes (supplied already filled with cell lysis buffer), and the solution is mixed to lyse red and white blood cells. Cell nuclei and mitochondria are pelleted by centrifugation, washed, and resuspended in digestion buffer. Protein contaminants are removed by incubation with a protease. DNA is precipitated in isopropanol, washed in 70% ethanol, dried, and resuspended in resuspension buffer.PerformanceDNA isolated using the PAXgene Blood DNA System has an A260/A280 ratio of 1.7–1.9. Fragments are 20–200 kb in size, with an average length of 50–150 kb (see figure "High quality and high molecular weight of genomic DNA"). Average DNA yields are 150–500 µg, depending on the number of nucleated cells present in the blood sample. Purified DNA can be used in PCR and quantitative, real-time PCR (see figure "Efficient multiplex PCR of 3 mitochondrial genes").Principle The PAXgene Blood DNA System is an integrated and standardized system for collection and stabilization of whole blood specimens and isolation of their genomic DNA. The system requires the combined use of PAXgene Blood DNA Tubes for blood collection and stabilization, followed by DNA isolation using the PAXgene Blood DNA Kit. Blood is collected into PAXgene Blood DNA Tubes, which contain a proprietary blend of reagents that both prevents blood coagulation and stabilizes white blood cells. Blood samples can be stored in the tubes for up to 14 days at room temperature. DNA is isolated from the tubes using appropriate buffers supplied in the PAXgene Blood DNA Kit. Highly pure genomic DNA is obtained, suitable for use in a wide range of downstream applications. Procedure The PAXgene Blood DNA procedure is shown in the flowchart "PAXgene Blood DNA procedure". Blood samples (8.5 ml) are collected in PAXgene Blood DNA Tubes, where they may be stored or transported. For DNA isolation, the blood is transferred to processing tubes (supplied already filled with cell lysis buffer), and the solution is mixed to lyse red and white blood cells. Cell nuclei and mitochondria are pelleted by centrifugation, washed, and resuspended in digestion buffer. Protein contaminants are removed by incubation with a protease. DNA is precipitated in isopropanol, washed in 70% ethanol, dried, and resuspended in resuspension buffer. ApplicationsThe DNA can be used in a wide range of downstream applications, including: PCR and quantitative, real-time PCR Southern blotting SNP genotyping Pharmacogenomic studies SNP discovery and Validation SpecificationsFeaturesSpecifications ApplicationsPCR, qPCR, real-time PCR, blotting, genotyping Elution volume1 ml FormatProcessing tube (salting out method) Main sample typeWhole blood ProcessingManual (centrifugation) Sample amount8.5 ml Time per run60 min/8 samples Yield150–500 µgProduct ResourcesYou are not authorized to download the resourceBrochures & Guides (3) Sort optionsSort alphabetically Analyzing Genetic Differences - (EN)Second edition — innovative toolsShow details(EN) - Analyzing Gene Expression and RegulationShow details(EN) - PAXgene Blood DNA System BrochureFor collection, storage, and transport of whole blood for genomic DNA isolationShow detailsShow allShow lessFAQs (17) Sort optionsSort alphabetically How do I safely inactivate biohazardous flow-through material?FAQ ID -12View3497 - What is the expected purity and size of DNA extracted using the PAXgene Blood DNA kit?FAQ ID - 3497ViewCan mitochondrial DNA be isolated with the PAXgene Blood DNA System?FAQ ID - 3498ViewDoes the DNA extracted using the PAXgene Blood DNA System contain RNA?FAQ ID - 3499ViewCan smaller volumes of blood be processed from the PAXgene Blood DNA Tube?FAQ ID - 3500ViewWhat does the resuspension buffer, Buffer BG4, in the PAXgene Blood DNA kit contain? FAQ ID - 3501ViewHow should DNA purified using the PAXgene Blood DNA System be stored?FAQ ID - 3502ViewIs it possible to interrupt the PAXgene Blood DNA kit protocol?FAQ ID - 3503ViewCan I use the PAXgene Blood DNA Tube to collect blood from animals?FAQ ID - 3504ViewCan I add bleach to the sample preparation waste?FAQ ID - 3505ViewWhat downstream applications have been tested with DNA purified using the PAXgene Blood DNA System?FAQ ID - 3506ViewHow should blood samples drawn into PAXgene Blood DNA Tubes be transported?FAQ ID - 3507ViewHow long can samples in PAXgene Blood DNA Tubes be stored at room temperature? FAQ ID - 3508ViewCan PAXgene Blood DNA Tubes be stored at higher than room temperature (18–25°C)? FAQ ID - 3509ViewWhat is the expected purity of DNA extracted from blood samples collected in PAXgene Blood DNA Tubes IVD? FAQ ID - 3511ViewWhich downstream applications have been tested with DNA purified from samples collected in PAXgene Blood DNA Tubes IVD?FAQ ID - 3512ViewWhere can I find additional information for PreAnalytiX PAXgene products? FAQ ID - 3515ViewShow allShow lessKit Handbooks (1) Sort optionsSort alphabetically (EN) -PAXgene Blood DNA Kit HandbookFor isolation of genomic DNA from 8.5 ml human whole bloodShow detailsShow allShow lessScientific Posters (1) Sort optionsSort alphabetically Reducing Variability and Improving Workflow in Collecting, Transporting, and Processing Blood Samples for Genomic DNA Purification Using the PAXgene Blood DNA System (EN)Groelz et al., ASHG 2004Show detailsShow allShow lessSupplementary Protocols (1) Sort optionsSort alphabetically Manual, High-Throughput Genomic DNA Purification Using the PAXgene Blood DNA System (EN)Show detailsShow allShow lessApplication Notes (10) Sort optionsSort alphabetically Integrity and quality of purified DNA obtained using the PAXgene Blood DNA System and stored for 10 years at 4°C or –20°CShow detailsDetection of Mitochondrial DNA in DNA Purified Using the PAXgene Blood DNA System (EN)Show detailsApplications of the PAXgene Blood DNA System (EN)Show detailsHigh-throughput DNA Purification Using the PAXgene Blood DNA System (EN)Show detailsStorage of Blood Samples Collected in PAXgene Blood DNA Tubes at 25°C (EN)Show detailsStorage of Blood Samples Collected in PAXgene Blood DNA Tubes at 2–8°C (EN)Show detailsStorage of Blood Samples Collected in PAXgene Blood DNA Tubes at 30°C (EN)Show detailsStorage of Blood Samples Collected in PAXgene Blood DNA Tubes at –20°C (EN)Show detailsStorage of Blood Samples Collected in PAXgene Blood DNA Tubes at –80°C (EN)Show detailsTransportation Study with Blood Samples Collected into PAXgene Blood DNA Tubes (EN)Show detailsShow allShow lessSafety Data Sheets (2) Sort optionsSort alphabetically MSDS PAXgene Blood DNA Kit (25)CofAShow allShow lessfragment fix placeholderCustomers who bought these products also boughtCat No./ID:761115PAXgene Blood DNA Tubes (100)100 PAXgene Blood DNA Tubes$162.00Add To CartCat No./ID:762164PAXgene Blood RNA Kit (50)50 PAXgene Spin Columns, 50 PAXgene Shredder Spin Columns, Processing Tubes, RNase-Free DNase I, RNase-Free Reagents and Buffers. 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