Sample Collection & StabilizationDNA5RNA19Tissue/FFPE5PAXgene Tissue STABILIZERPrint For stabilization of tissue specimens previously fixed in PAXgene Tissue FIX Preservation of bothmorphology and biomolecules Tissue can be stored and archived for later processing RNA, miRNA, DNA, and/orproteins from one sample PAXgene Tissue STABILIZER is used for transport or storage of tissue specimens fixed with the PAXgene Tissue FIX Container (50 ml). After fixation of tissue samples with the PAXgene Tissue FIX Container (50 ml), PAXgene Tissue FIX is removed and replaced by PAXgene Tissue STABILIZER within the same container. Nucleic acids, proteins and morphology of the sample are now stable up to 7 days at room temperature, or for longer periods at 2–8°C, or even at –20°C and –80°C. Stabilized samples can be embedded in paraffin for histological studies. PAXgene Tissue Kits and supplementary protocols provide efficient subsequent purification of RNA, miRNA, DNA, and/or proteins from the same sample. PAXgene Tissue STABILIZER Concentrate is only to be used in conjunction with tissue specimens previously fixed in PAXgene Tissue FIX. Buy ProductsProduct DetailsProduct ResourcesBuy ProductsCat No./ID:765512PAXgene Tissue STABILIZER Concentrate (150 ml)$237.00Order Product8 bottles of PAXgene Tissue Stabilizer concentrate, for 4 liters of PAXgene Tissue STABILIZERFor Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.Product Details High-quality DNA from PFPE tissue with preserved morphology.[A] Hematoxylin and eosin (H&E) staining of PAXgene Tissue-fixed, paraffin-embedded (PFPE) human colorectal cancer tissue and [B] DNA on agarose gel electrophoresis using 0.8% TBE buffered gels with 200 ng genomic DNA isolated in triplicate from 5 cases (#1–5) of human PFPE colorectral cancer. M: markers.Absorbance ratio of high-quality, high molecular weight DNA from 12 different PAXgene Tissue-fixed, paraffin-embedded (PF) tissue types, processed on the QIAcube.Twelve different PAXgene Tissue-fixed (PF) rat tissue types, including fibrous or fatty, DNA-rich and DNA-poor tissue, processed with the PAXgene Tissue DNA Kit on the QIAcube in a single run. Ratio of absorbance at 260 and 280 nm.Comparison of manual and automated procedure: DNA yield from sections of PAXgene Tissue-fixed, paraffin-embedded (PFPE) tissue.DNA yield from 3 sections (thickness 10 µm) each of 8 different PAXgene Tissue-fixed, paraffin-embedded (PFPE) rat tissue types processed in triplicate with the PAXgene Tissue DNA Kit on the QIAcube or manually.Comparison of manual and automated procedure: Absorbance ratio from sections of PAXgene Tissue-fixed, paraffin-embedded (PFPE) tissue.Ratio of absorbance at 260 and 280 nm from 3 sections (thickness 10 µm) each of 8 different PAXgene Tissue-fixed, paraffin-embedded (PFPE) rat tissue types processed in triplicate with the PAXgene Tissue DNA Kit on the QIAcube or manually.DNA without chemical modification can be used for demanding downstream applications.Multiplex and long-range PCR of DNA from PAXgene Tissue-fixed, paraffin-embedded (PFPE) human colorectal cancer tissue (modified according to Viertler et al., (2012) A new technology for stabilization of biomolecules in tissues for combined histological and molecular analyses. J. MOl. Diagn. 14, 458). [A] Multiplex PCR of 8 different genomic DNA fragments ranging from 22 to 955 bp. [B] Long-range PCR of a 5 kb genomic DNA fragment.Protein extracted from PFPE tissue is suitable for two-dimentional gel electrophoresis.Non-malignant human duodenum tissue was divided into 3 samples and either flash-frozen in liquid nitrogen (cryo), or prepared as PFPE or FFPE tissue. Proteins from cryo and PFPE tissue were extracted in 2D buffer (30 mM Tris-HCl, pH 8.8, 7 M urea, 2 M thiourea, 4% CHAPS, 75 mM DTT) supplemented with protease inhibitor. Proteins from FFPE tissue were extracted in EXB Plus buffer supplemented with protease inhibitor, precipitated with acetone and resuspended in 2D buffer (as described in Guendisch et al. 2013). Samples (150 µg) were separated by 2-D PAGE. Data kindly provided by Karl-Friedrich Becker, Technical University of Munich, Germany.Agarose gel electrophoresis of high-quality, high molecular weight DNA from 12 different PAXgene Tissue-fixed (PF) tissue types, processed on the QIAcube.Twelve different PAXgene Tissue-fixed (PF) rat tissue types, including fibrous or fatty, DNA-rich and DNA-poor tissue, processed with the PAXgene Tissue DNA Kit on the QIAcube in one single run. DNA from each tissue type (300 ng) on agarose gel electrophoresis using 1% TBE buffered gels; M: markers.Comparison of manual and automated procedure: Agarose gel electrophoresis from sections of PAXgene Tissue-fixed, paraffin-embedded (PFPE) tissue.Agarose gel electrophoresis from 3 sections (thickness 10 µm) each of 8 different PAXgene Tissue-fixed, paraffin-embedded (PFPE) rat tissue types processed in triplicate with the PAXgene Tissue DNA Kit on the QIAcube and manually. DNA from each replicate and tissue type (200 ng) on agarose gel electrophoresis using 1% TBE buffered gels; M: markers.Detection of nondegraded, immunoreactive phosphoproteins from human clinical PFPE tissue.Non-malignant human uterus, breast, prostate and bladder tissue specimens were divided into 3 samples and either flash-frozen in liquid nitrogen (cryo), PAXgene Tissue-fixed, paraffin-embedded (PFPE) or formalin-fixed, paraffin-embedded (FFPE). Proteins from cryo, PFPE and FFPE tissues were extracted using the extraction buffer EXB Plus (Qproteome FFPE Tissue Kit; described in Ergin et al. 2010, Guendisch et al. 2013 and PAXgene Tissue supplementary protocols). SDS-PAGE and Western blot analysis were performed with 15 µg protein and the indicated antibodies. Data kindly provided by Karl-Friedrich Becker, Technical University of Munich, Germany.High concordance of miRNA expression between total RNA isolated from PFPE and fresh-frozen tissue.RNA, including miRNA, was purified from mirrored human breast cancer tissue fresh frozen in liquid nitrogen using the QIAGEN miRNeasy Kit, or from sections of PAXgene Tissue-fixed, paraffin-embedded (PFPE) tissue using the PAXgene Tissue RNA/miRNA Kit. Shown is a scatterplot of CT values from different single miRNA-specific RT-qPCR assays using the QIAGEN miScript System: miR9, -10a, -10b, -29a, -103, -125b, -143, -145, -192 and miScript PCR controls RNUA1, RNU5A, RNU6B, SNORD25, SCARNA19, SNORA73A; R2: coefficient of determination.RNA purified without chemical modification from PFPE tissue using the PAXgene Tissue RNA/miRNA Kit.SYBR Green real-time RT-qPCR was performed with 10 ng RNA from cryopreserved, formalin-fixed, paraffin-embedded (PPFE) or PAXgene Tissue-fixed, paraffin-embedded (PFPE) rat tissue (modified according to Groelz et al. 2013). Depicted are the average delta-CT values (delta-CT = CT[FFPE] – CT[cryo] or delta-CT = CT[PFPE] – CT[cryo]) from 6 different assays with amplicons ranging from 109 to 465 bp.Immunohistochemistry (IHC) staining with the PAXgene Tissue System gives comparable results to formalin-fixed tissue.Human palatine tonsil tissue was fixed in PAXgene Tissue FIX or with neutral-buffered formalin and embedded in paraffin. Primary antibodies to the indicated antigens were linked to a streptavidin-peroxidase conjugate by a biotinylated secondary antibody (LSAB method). Sectiosn were counterstained with hematoxylin. PFPE: PAXgene Tissue-fixed, paraffin-embedded. FFPE: formalin-fixed, paraffin-embedded.H&E staining with the PAXgene Tissue System gives results comparable to formalin-fixed tissue.Human tissue samples were divided into 2 sub-samples. One sub-sample was fixed with PAXgene Tissue FIX and the other was fixed with neutral-buffered formalin. The fixed tissues were embedded in paraffin, sectioned and stained with hematoxylin and eosin. PFPE: PAXgene Tissue-fixed, paraffin-embedded. FFPE: formalin-fixed, paraffin-embedded.The PAXgene Tissue FIX Container (50 ml) and PAXgene Tissue STABILIZER workflow.Single-chamber container for fixation and stabilization of a single, larger or multiple, smaller tissue samples.Yield of high-quality, high molecular weight DNA from 12 different PAXgene Tissue-fixed (PF) tissue types, processed on the QIAcube.Twelve different PAXgene Tissue-fixed (PF) rat tissue types, including fibrous or fatty, DNA-rich and DNA-poor tissues, processed with the PAXgene Tissue DNA Kit on the QIAcube in one single run. DNA yield per 10 mg tissue.PerformanceThe fixation and stabilization method preserves tissue morphology and the integrity of nucleic acids without destructive crosslinking and degradation found in formalin-fixed tissues (see figure "Preservation of tissue morphology").When fixed tissue is stored in PAXgene Tissue STABILIZER, the nucleic acids, proteins, and morphology of the tissue sample are stable for up to 7 days at room temperature (15–25°C) or for up to 4 weeks at 2–8°C. Tissue samples can even be stored in the PAXgene Tissue STABILIZER for longer periods at –20°C (–15°C to –30°C) or –80°C (–65°C to –90°C) without negative effects on the morphology of the tissue or the integrity of the nucleic acids.Specifications for storage conditions in PAXgene Tissue STABILIZER were determined using animal tissues. PrincipleThe methods for tissue fixation currently used in traditional histology are of limited use for molecular analysis. Fixatives that contain formaldehyde crosslink biomolecules and modify nucleic acids and proteins. Such crosslinks lead to nucleic acid degradation during tissue fixation, storage, and processing. Since they cannot be removed completely, the resulting chemical modifications can cause inhibition in downstream applications, such as quantitative PCR or RT-PCR. To enable both molecular and traditional pathology testing from the same specimen, a method is needed to stabilize molecular content and preserve morphology.To meet this need, PreAnalytiX has developed the PAXgene Tissue System. The system consists of a fixation reagent (PAXgene Tissue FIX), a stabilization reagent (PAXgene Tissue STABILIZER), prefilled containers for tissue collection, storage, and transportation, and kits for purification of RNA, DNA, or total RNA, including miRNA. In addition, supplementary protocols for protein purification and other applications are available in the "Resources" section of this page or at www.preanalytix.com.PAXgene Tissue reagents in prefilled containers and PAXgene Tissue Kits provide a complete preanalytical solution for collection, fixation, and stabilization of tissue, and purification of high-quality nucleic acids for molecular research analysis.ProcedureAfter fixation of tissue samples with the PAXgene Tissue FIX Container (50 ml), the fixative in the container is removed and replaced by PAXgene Tissue STABILIZER within the same container. Stabilized samples can be embedded in paraffin for histological studies. Nucleic acids and proteins can be isolated from the stabilized samples before or after embedding in paraffin. See the PAXgene Tissue Kit Handbooks for information about DNA, RNA, or miRNA, and the PAXgene Tissue supplementary protocols in the "Resources" section of this page or atwww.preanalytix.com for protein purification and other applications. ApplicationsSections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue can be used for histological studies or extraction of nucleic acids or proteins. Purification of RNA, total RNA, including miRNA, or DNA from PAXgene Tissue fixed and stabilized tissue samples requires the use of one of the PAXgene Tissue Kits for RNA, miRNA, or DNA. Purification of protein requires the Qproteome FFPE Kit.Product ResourcesYou are not authorized to download the resourceBrochures & Guides (5) Sort optionsSort alphabetically DNA Isolation with the PAXgene Tissue DNA Kit (EN)For isolation and purification of genomic DNA from tissue samples stabilized using the PAXgene Tissue SystemShow detailsHistological staining with the PAXgene Tissue System (EN)Show detailsProtein Extraction from PFPE - (EN)For extraction of full-length proteins from PFPE tissue using the Qproteome FFPE Tissue KitShow detailsRNA Isolation with the PAXgene Tissue RNA Kit (EN)For isolation and purification of intracellular RNA from tissue samples stabilized using the PAXgene Tissue SystemShow detailsPAXgene Tissue System Brochure (EN)Moving toward excellence and standardization in tissue collection and fixationShow detailsShow allShow lessFAQs (34) Sort optionsSort alphabetically What is the maximum tissue size that can be fixed in a PAXgene Tissue Container?FAQ ID -2518ViewHow long is the fixation time?FAQ ID -2519ViewIs it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue blocks?FAQ ID -2524ViewIs it possible to embed PAXgene Tissue fixed and stabilized samples in Optimal Cutting Temperature (OCT) medium for freezing?FAQ ID -2525ViewIs the morphology after H&E staining comparable to formalin-fixed samples?FAQ ID -2526ViewCan PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for in situ hybridization?FAQ ID -2529ViewCan sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for other histochemical staining techniques, such as PAS?FAQ ID -2530ViewIs it possible to microdissect PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissues?FAQ ID -2531ViewWhat is the purity of nucleic acids extracted with the PAXgene Tissue kits?FAQ ID -2533ViewWhat is the average RNA yield from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?FAQ ID -2534ViewWhat is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?FAQ ID -2538ViewWhat does PAXgene Tissue STABILIZER contain?FAQ ID - 3513ViewAre PAXgene Tissue Containers suitable for long term-storage at freezing temperatures? FAQ ID -3030ViewIs it possible to use a standard processor — the kind used routinely for formalin-fixed samples — for dehydration and paraffin infiltration of PAXgene Tissue treated samples?FAQ ID -3032ViewWhich fixation method is used in the PAXgene Tissue System?FAQ ID - 3600ViewWhat is the fixation reagent of PAXgene Tissue FIX?FAQ ID - 3601ViewWhat is the stabilization reagent of PAXgene Tissue STABILIZER?FAQ ID - 3602ViewWhy are two reagents used in the PAXgene Tissue System?FAQ ID - 3603ViewWhat is the maximum tissue size that can be fixed in a PAXgene Tissue FIX Container (50 ml)?FAQ ID - 3604ViewWhich conditions are recommended for the storage of tissues in PAXgene Tissue STABILIZER?FAQ ID - 3605ViewIs it possible to process formalin-fixed and PAXgene Tissue fixed samples together in one run?FAQ ID - 3606ViewIs it necessary to clean a processor normally used for formalin-fixed tissue before using it with PAXgene Tissue fixed tissue?FAQ ID - 3607ViewIs it possible to integrate the PAXgene Tissue STABILIZER into tissue processing?FAQ ID - 3608ViewAre immunohistochemistry (IHC) assays developed for formalin-fixed, paraffin-embedded (FFPE) tissues compatible with PAXgene Tissue fixed, paraffin-embedded (PFPE) tissues?FAQ ID - 3609ViewIs it possible to use formalin-fixed, paraffin-embedded (FFPE) kits and protocols to isolate biomolecules from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?FAQ ID - 3610ViewHow well is RNA integrity preserved in PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?FAQ ID - 3611ViewHow well is DNA integrity preserved in PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?FAQ ID - 3612ViewWhat are the yield and integrity of nucleic acids isolated from blocks of PAXgene Tissue fixed, cryo-embedded (PFCE) tissue?FAQ ID - 3613ViewAre special kits and protocols required to isolate biomolecules from PAXgene Tissue fixed, cryo-embedded (PFCE) tissue? FAQ ID - 3614ViewDoes the PAXgene Tissue RNA Kit co-purify small RNAs?FAQ ID - 3615ViewCan proteins be extracted from PAXgene Tissue fixed specimens?FAQ ID - 3616ViewWhich kits and protocols can be used to isolate nucleic acids from microdissected PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) specimens?FAQ ID - 3617ViewWhat is the PCR performance of DNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissue compared to DNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?FAQ ID - 3618ViewHow is the quality of proteins purified from PAXgene Tissue fixed and stabilized tissue?FAQ ID - 3619ViewShow allShow lessKit Handbooks (2) Sort optionsSort alphabetically PAXgene Tissue FIX Container HandbookFor collection, fixation, and stabilization of multiple small tissue samples or a single large tissue sampleShow detailsPAXgene Tissue STABILIZER Reagent Product SheetFor stabilization of tissue specimens fixed in PAXgene Tissue FIX ContainerShow detailsShow allShow lessScientific Posters (14) Sort optionsSort alphabetically DNA Quality Measurement and Somatic Mutation Profiling in PAXgene Tissue Samples with qBiomarker Somatic Mutation PCR Arrays (EN)Long et al., ADAPT 2012Show detailsEvaluation of PAXgene Tissue System: preservation of morphology and gene expression in human melanoma (EN)Hesse et al., AACR-NCI-EORTC 2011Show detailsPreservation of gene expression profile and histomorphology in human breast tumor tissue with the new PAXgene Tissue System (EN)Groelz et al., AACR 2010Show detailsMorphological, Epigenomic, and Mutational Analyses of PAXgene Tissue Fixed, Paraffin-embedded (PFPE) Colorectal Cancer (CRC) Specimens (EN)Groelz et al.,AMP 2010Show detailsNew fixation technology for simlutaneous preservation of morphology and nucleic acids in tissue (EN)Groelz et al., AMP 2008Show detailsPreservation of histomorphology and nucleic acids in human breast tumor tissue with the new PAXgene Tissue System (EN)Groelz et al.,AMP 2009Show detailsPreservation of morphology and biomolecules within tissue stored for three years at –80°C in PAXgene Tissue Stabilizer Reagent (EN)Groelz et al., ISBER 2012Show detailsPAXgene vs. formalin fixed tissue: a comparison of tissue morphology and RNA quality (EN)Groelz et al.,BRN Symposium 2012Show detailsPAXgene tissue fixation technology for simultaneous preservation of morphology and biomolecules (EN)Groelz et al., ECP 2012Show detailsA new tissue fixative for biomarker discovery: Gene expression and miRNA in PFPE colorectal cancer (CRC) and breast cancer tissue vs. FFPE tissue (EN)Groelz et al.,3rd Annual Oncology Biomarkers 2011Show detailsRT-PCR performance of RNA obtained from archived FFPE and PFPE blocks of tissue (EN)Groelz et al., AMP 2014Show detailsPAXgene Tissue: A new fixation technology for simultaneous preservation of morphology and nucleic acids (EN)Groelz et al.,BRN Symposium 2011Show detailsStability of nucleic acids in archived formalin and PAXgene Tissue fixed, paraffin-embedded blocks of tissue (EN)Groelz et al., ECP 2014Show detailsLong-term storage of tissue specimens at –20­°C to –80°C with preservation of morphology and nucleic acids with frozen tissue (EN)Groelz et al.,AMP 2009Show detailsShow allShow lessSupplementary Protocols (16) Sort optionsSort alphabetically Simultaneous purification of genomic DNA and total RNA, including miRNA, from sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue (EN)Show detailsSimultaneous purification of genomic DNA and total RNA from sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue (EN)Show detailsDeparaffinization of PAXgene® Tissue fixed, paraffin-embedded tissue sections with Deparaffinization Solution - (EN)Show detailsManual processing of tissue specimens treated with the PAXgene® Tissue System - (EN)Show detailsCryo-embedding tissue specimens fixed and stabilized with the PAXgene® Tissue System - (EN)Show detailsPurification of genomic DNA from microdissected PAXgene® Tissue-fixed, paraffin-embedded (PFPE) and PAXgene Tissue-fixed, cryo-embedded (PFCE) tissues - (EN)Show detailsPurification of genomic DNA from sections of PAXgene® Tissue fixed, cryo-embedded (PFCE) tissue placed directly into a microcentrifuge tube - (EN)Show detailsPurification of total RNA from microdissected PAXgene® Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissues - (EN)Show detailsPurification of total RNA from sections of PAXgene® Tissue fixed, cryo-embedded (PFCE) tissue placed directly into a microcentrifuge tube - (EN)Show detailsPurification of total RNA, including miRNA, from microdissected PAXgene® Tissue fixed, paraffin embedded (PFPE) and PAXgene Tissue fixed, cryo-cryoembedded (PFCE) tissues - (EN)Show detailsPreparation of sections from PAXgene® Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissues for manual or laser microdissection (LMD) - (EN)Show detailsPreparation of PFPE tissue sections for use with in situ hybridization (ISH) staining assays - (EN)Show detailsPurification of total RNA, including miRNA, from sections of PAXgene® Tissue fixed, cryo-embedded (PFCE) tissue placed directly into a microcentrifuge tube - (EN)Show detailsPurification of full-length proteins from PAXgene® Tissue fixed and stabilized (PF) tissue samples - (EN)Show detailsPurification of full-length proteins from sections of PAXgene® Tissue fixed, cryo-embedded (PFCE) tissue - (EN)Show detailsPurification of full-length proteins from sections of PAXgene® Tissue fixed, paraffin-embedded (PFPE) tissue - (EN)Show detailsShow allShow lessTechnical Information (9) Sort optionsSort alphabetically Effect of epitope retrieval conditions on immunohistochemical staining of PFPE tonsil tissue with anti-human Ki-67 antigen (clone MIB-1) (EN)Show detailsMorphology and RNA preservation in PAXgene Tissue fixed, paraffin-embedded tissue (PFPE) stored for 18 months at different temperatures (EN)Show detailsInfluence of formalin contamination during processing of PAXgene Tissue fixed, paraffin-embedded tissue (PFPE) on RNA yield, integrity, and performance in quantitative RT-PCR (EN)Show detailsInfluence of modifications to processing protocol on RNA yield and integrity (EN)Show details(EN) - Simultaneous perservation of RNA and morphology in tissue samples fixed with PAXgene Tissue Fix and stored for up to 2 years in PAXgene Tissue Stabilizer at –20°C or –80°CShow detailsVacuum sealing of fixed and stabilized tissue with a FoodSaver Vacuum Sealer for dry and safe transport (EN)Show detailsQuantitative analysis of KRAS and BRAF mutational status in DNA from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue using Pyrosequencing technology (EN)Show detailsRNA stability in tissue samples fixed and stabilized with the PAXgene Tissue System for up to 7 days at 22°C or 2 months at 4°C (EN)Show detailsYield, purity, and integrity of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) rat tissue (EN)Show detailsShow allShow lessSafety Data Sheets (1) Sort optionsSort alphabetically MSDS PAXgene Tissue STABILIZER Concentrate (150 ml)Show allShow less References 0 fragment fix placeholderCustomers who bought these products also boughtCat No./ID:765312PAXgene Tissue FIX Container (50 ml)For fixation and stabilization of tissue specimen: 10 prefilled Reagent Containers containing 50 ml of PAXgene Tissue FIX$171.00Add To CartCat No./ID:767134PAXgene Tissue DNA Kit (50)For 50 DNA preps: PAXgene DNA Mini Spin Columns, Processing Tubes, Microcentrifuge Tubes, Carrier RNA, and Buffers; to be used in conjunction with PAXgene Tissue Containers$296.00Add To CartCat No./ID:766134PAXgene Tissue RNA/miRNA Kit (50)For 50 RNA preps: PAXgene RNA MinElute Spin Columns, PAXgene Shredder Spin Columns, Processing Tubes, Microcentrifuge Tubes, Carrier RNA, RNase-Free DNase, and RNase-Free Buffers; to be used in conjunction with PAXgene Tissue Containers$578.00Add To CartCat No./ID:292035 mericon Chicken Kit (96) For96 reactions: PCR Assay Chicken, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-Free Water, 50x ROX Dye Solution$1,065.00Add To CartCat No./ID:150065REPLI-g WTA Single Cell Kit (96)REPLI-g SensiPhi DNA Polymerase, Buffers, and Reagents for 96 x 60 µl whole transcriptome amplification reactions (typical yield: 20 µg)$4,510.00Add To CartCat No./ID:292065 mericon Sheep Kit (96)For 96 reactions: PCR Assay Sheep, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-Free Water, 50x ROX Dye Solution$1,070.00Add To CartCat No./ID:292015 mericon Pig Kit (96) For 96 reactions: PCR Assay Pig, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water$1,057.00Add To CartCat No./ID:204374Rotor-Gene Probe PCR Kit (400)For 400 x 25 µl reactions: 3 x 1.7 ml 2x Rotor-Gene Probe PCR Master Mix, 2 x 2 ml RNase-Free Water$489.00Add To CartCat No./ID:28706X4QIAquick Gel Extraction Kit (1000)For gel extraction or cleanup of 1000 reactions: 1000 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)$1,879.00Add To CartCat No./ID:181942GeneRead DNAseq Panel PCR Kit V2 (96)GeneRead HotStarTaq polymerase, GeneRead DNAseq Panel 5x PCR Buffer, Nuclease-free Water; enough for 96 samples (4-pool panels) or 192 samples (1-pool panels)$886.00Add To CartCat No./ID:158767Gentra Puregene Cell Kit (8 x 108)For (8 x 108) cells: RNase A Solution and Reagents$282.00Add To CartCat No./ID:204643QuantiTect Multiplex RT-PCR Kit (200)For 200 x 50 µl reactions: 3 x 1.7 ml 2x QuantiTect Multiplex RT-PCR Master Mix (with ROX dye), 100 µl QuantiTect Multiplex RT Mix, 2 x 2 ml RNase-Free Water$1,037.00Add To CartCat No./ID:20902QIAEX II Suspension (1.5 ml)1.5 ml suspension$177.00Add To CartCat No./ID:30600Ni-NTA Fast Start Kit (6)For purification and detection of six 6xHis-tagged protein preps: 6 x Fast Start Columns, Penta·His Antibody, Buffers and Reagents$367.00Add To CartCat No./ID:35061Ni-NTA HisSorb Plates (5)5 Ni-NTA-coated, transparent 96-well plates$254.00Add To CartCat No./ID:974203PyroMark Q48 Magnetic Beads (300)Magnetic streptavidin-coated Sepharose beads for running 300 PyroMark Q48 Autoprep reactions$148.00Add To CartCat No./ID:217184miRNeasy Serum/Plasma Kit (50)For 50 total RNA preps: 50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml and 2 ml), QIAzol Lysis Reagent, Ce_miR-39_1 miScript Primer Assay, RNase-free Reagents and Buffers$489.00Add To CartCat No./ID:979201PyroMark Q24 Plate (100)24-well plate for samples, for use with PyroMark Q24 and PyroMark Q24 Advanced; 100 plates in each package$249.00Add To Cart