RNACell-Free RNA6Microbial RNA7miRNA10mRNA9RNA Clean Up3Total RNA31PAXgene Tissue RNA/miRNA KitPrint For purification of microRNA and total RNA from tissues fixed and stabilized in PAXgene Tissue ContainersIntegrated fixation, stabilization, and purificationEffective purification of miRNA and total RNAHigh-quality miRNA from tissues Preserved tissue morphology Tissue samples fixed and stored in PAXgene Tissue Containers can be paraffin-embedded and used for pathological studies as well as for subsequent purification of miRNA, RNA, and/or DNA. The PAXgene Tissue miRNA Kit provides purification of total RNA, including RNA from approximately 18 nucleotides, from tissues fixed and stabilized in PAXgene Tissue Containers. Purification is carried out using silica-based RNA purification technology in a spin-column format. Used with the containers, the kit provides a complete preanalytical solution for collection, fixation, and stabilization through to purification of high-quality miRNA and total RNA for molecular analysis. Buy ProductsProduct DetailsSpecificationsProduct ResourcesBuy ProductsCat No./ID:766134PAXgene Tissue RNA/miRNA Kit (50)$578.00Order ProductFor 50 RNA preps: PAXgene RNA MinElute Spin Columns, PAXgene Shredder Spin Columns, Processing Tubes, Microcentrifuge Tubes, Carrier RNA, RNase-Free DNase, and RNase-Free Buffers; to be used in conjunction with PAXgene Tissue ContainersFor Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.Product Details The PAXgene Tissue RNA/miRNA Procedure.Disruption and homogenization of the tissue sample is performed in binding buffer, Buffer TM1. After centrifugation to remove residual cell debris, isopropanol is added to the lysate to provide appropriate binding conditions for all RNA molecules 18 nucleotides and longer. The sample is then applied to a PAXgene RNA MinElute spin column, where total RNA binds to the membrane and contaminants are efficiently washed away. Between the first and the second wash step, the membrane is treated with DNase I to remove trace amounts of bound DNA. After the wash steps, RNA, including miRNA, is eluted in a low-salt elution buffer and denatured by heating.The PAXgene Tissue miRNA procedure.Efficient purification of miRNA from fixed tissue stored in PAXgene Tissue Containers.Tissues from different organs were fixed for 3 hours in PAXgene Tissue Fix, transferred to PAXgene Tissue Stabilizer, and stored for 24 hours at room temperature. After processing, total RNA was purified from sections of paraffin-embedded tissue using the PAXgene Tissue RNA Kit (RNA/miRNA). miRNA-enriched fractions (200 nt) were also isolated from sections of the same tissue blocks using the PAXgene Tissue miRNA Kit (Enriched miRNA). Purified RNA was used as a template in quantitative, real-time RT-PCR assays for the miRNA miR-10a using the miScript Reverse Transcription Kit and the miScript SYBR® Green PCR Kit.Performance Together the container and kit provide a complete preanalytical solution for collection, fixation, and stabilization of tissue, and for purification of high-quality RNA, including miRNA, for molecular analysis (see figure "Efficient purification of miRNA from fixed tissue stored in PAXgene Tissue Containers"). Total RNA purified using the PAXgene Tissue miRNA Kit is highly pure. Genomic DNA contamination is minimized, and purified RNA is ready to use in downstream applications with no detectable PCR inhibition. All RNA molecules longer than 18 nucleotides are purified. Principle Current tissue fixation methods used in traditional histology are of limited use for molecular analysis. Fixatives that contain formaldehyde cross-link biomolecules and modify nucleic acids and proteins. During tissue fixation, storage, and processing, cross-links lead to degradation of nucleic acids. Since cross-links can not be removed completely, the resulting chemical modifications can cause inhibition in sensitive downstream applications such as quantitative PCR or RT-PCR. In order to enable both molecular and traditional pathology testing from the same specimen, a method is needed for stabilization of molecular content and preservation of morphology. PreAnalytiX has developed the PAXgene Tissue System to meet such needs. The system consists of a tissue collection device (the PAXgene Tissue Container for collection, stabilization, storage, and transportation of human tissue specimens) and kits for purification of miRNA, total RNA, or DNA. PAXgene Tissue Containers provide tissue fixation for histopathology studies and enable purification of high-quality nucleic acids from the same sample for molecular analysis. The fixation and stabilization method preserves tissue morphology and the integrity of nucleic acids without destructive cross-linking and degradation found in formalin-fixed tissues. For purification of total RNA including miRNA, the system requires the use of PAXgene Tissue Containers for tissue collection and stabilization, followed by RNA isolation and purification using the PAXgene Tissue miRNA Kit.Procedure PAXgene Tissue Containers are dual-chamber containers prefilled with 2 reagents. PAXgene Tissue Fix rapidly penetrates and fixes the tissue. After fixation, the tissue is removed from the PAXgene Tissue Fix and transferred to PAXgene Tissue Stabilizer in the second chamber of the same container. When the tissue is stored in PAXgene Tissue Stabilizer, nucleic acids and morphology of the tissue sample are stable for a minimum of 3 and a maximum of 7 days at room temperature or for up to 8 weeks at 2–8°C, depending on the type of tissue. Storage at –15 to –30°C is also possible for at least 26 months without any negative effects on the morphology of the tissue or the integrity of the nucleic acids. Stabilized samples can be embedded in paraffin for histological studies. Nucleic acids can be isolated from the PAXgene Tissue fixed, paraffin-embedded (PFPE) samples either before or after embedding in paraffin. The PAXgene Tissue miRNA Kit provides 3 protocols for purification of total RNA from tissue fixed and stabilized in PAXgene Tissue Containers, including RNA molecules smaller than 200 nucleotides, such as 5.8S rRNA, 5S rRNA, tRNAs, and miRNAs. Optimized binding and washing conditions ensure the purification of RNA molecules as small as 18 nucleotides. As a prerequisite, the tissue must be fixed and stabilized in PAXgene Tissue Containers. Disruption and homogenization of the tissue sample is performed in the binding buffer, Buffer TM1. After a centrifugation step to remove residual cell debris, ethanol is added to the lysate to provide appropriate binding conditions for all RNA molecules 18 nucleotides and longer. The sample is then applied to a PAXgene RNA MinElute spin column where the total RNA binds to the membrane and contaminants are efficiently washed away. Between the first and second wash steps, the membrane is treated with DNase I to remove trace amounts of bound DNA. After the wash steps, RNA including miRNA is eluted in a low-salt elution buffer and denatured by heating (see figure "The PAXgene Tissue miRNA procedure"). Specifications for fixation and storage conditions in PAXgene Tissue Fix and PAXgene Tissue Stabilizer were determined using animal tissues.ApplicationsThe purified miRNA and total RNA is ready to use in a wide range of downstream applications, including: Northern blot analysis RT-PCR and quantitative, real-time RT-PCR Microarray analysis SpecificationsFeaturesSpecifications ApplicationsNorthern blot analysis, RT-PCR and quantitative, real-time RT-PCR, microarray analysis Elution volume14–40 µl FormatSpin column Main sample typeHuman tissue ProcessingManual (centrifugation) Sample amount4 x 15 x 15 mm TechnologySilica technology Time per run60 min + 15 min incubation/8 samples YieldDepends on tissue type and starting material (fixed or PFPE*) * PAXgene Fixed Paraffin Embedded. Product ResourcesYou are not authorized to download the resourceBrochures & Guides (3) Sort optionsSort alphabetically PAXgene Tissue - (EN)Two worlds in one sampleShow detailsAll insights start with the sample: Your comprehensive guide for isolating top-quality RNAShow detailsPAXgene Tissue System Brochure (EN)Moving toward excellence and standardization in tissue collection and fixationShow detailsShow allShow lessFAQs (6) Sort optionsSort alphabetically What can I use to isolate RNA smaller than 200 nucleotides?FAQ ID -115ViewIs a special processing protocol needed?FAQ ID -2523ViewIs it possible to microdissect PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissues?FAQ ID -2531ViewWhat is the purity of nucleic acids extracted with the PAXgene Tissue kits?FAQ ID -2533ViewWhat is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?FAQ ID -2538ViewWhere can I find additional information for PreAnalytiX PAXgene products? FAQ ID - 3515ViewShow allShow lessKit Handbooks (1) Sort optionsSort alphabetically PAXgene Tissue miRNA Kit Handbook - (EN)For isolation and purification of total RNA, including miRNA, from tissue samples stabilized in PAXgene Tissue ContainersShow detailsShow allShow lessScientific Posters (12) Sort optionsSort alphabetically Evaluation of PAXgene Tissue System: preservation of morphology and gene expression in human melanoma (EN)Hesse et al., AACR-NCI-EORTC 2011Show detailsPreservation of gene expression profile and histomorphology in human breast tumor tissue with the new PAXgene Tissue System (EN)Groelz et al., AACR 2010Show detailsNew fixation technology for simlutaneous preservation of morphology and nucleic acids in tissue (EN)Groelz et al., AMP 2008Show detailsPreservation of histomorphology and nucleic acids in human breast tumor tissue with the new PAXgene Tissue System (EN)Groelz et al.,AMP 2009Show detailsPreservation of morphology and biomolecules within tissue stored for three years at –80°C in PAXgene Tissue Stabilizer Reagent (EN)Groelz et al., ISBER 2012Show detailsPAXgene vs. formalin fixed tissue: a comparison of tissue morphology and RNA quality (EN)Groelz et al.,BRN Symposium 2012Show detailsPAXgene tissue fixation technology for simultaneous preservation of morphology and biomolecules (EN)Groelz et al., ECP 2012Show detailsA new tissue fixative for biomarker discovery: Gene expression and miRNA in PFPE colorectal cancer (CRC) and breast cancer tissue vs. FFPE tissue (EN)Groelz et al.,3rd Annual Oncology Biomarkers 2011Show detailsRT-PCR performance of RNA obtained from archived FFPE and PFPE blocks of tissue (EN)Groelz et al., AMP 2014Show detailsPAXgene Tissue: A new fixation technology for simultaneous preservation of morphology and nucleic acids (EN)Groelz et al.,BRN Symposium 2011Show detailsStability of nucleic acids in archived formalin and PAXgene Tissue fixed, paraffin-embedded blocks of tissue (EN)Groelz et al., ECP 2014Show detailsLong-term storage of tissue specimens at –20­°C to –80°C with preservation of morphology and nucleic acids with frozen tissue (EN)Groelz et al.,AMP 2009Show detailsShow allShow lessSupplementary Protocols (7) Sort optionsSort alphabetically Simultaneous purification of genomic DNA and total RNA, including miRNA, from sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue (EN)Show detailsDeparaffinization of PAXgene® Tissue fixed, paraffin-embedded tissue sections with Deparaffinization Solution - (EN)Show detailsManual processing of tissue specimens treated with the PAXgene® Tissue System - (EN)Show detailsPurification of total RNA from microdissected PAXgene® Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissues - (EN)Show detailsPurification of total RNA from sections of PAXgene® Tissue fixed, cryo-embedded (PFCE) tissue placed directly into a microcentrifuge tube - (EN)Show detailsPurification of total RNA, including miRNA, from microdissected PAXgene® Tissue fixed, paraffin embedded (PFPE) and PAXgene Tissue fixed, cryo-cryoembedded (PFCE) tissues - (EN)Show detailsPurification of total RNA, including miRNA, from sections of PAXgene® Tissue fixed, cryo-embedded (PFCE) tissue placed directly into a microcentrifuge tube - (EN)Show detailsShow allShow lessTechnical Information (7) Sort optionsSort alphabetically Morphology and RNA preservation in PAXgene Tissue fixed, paraffin-embedded tissue (PFPE) stored for 18 months at different temperatures (EN)Show detailsInfluence of formalin contamination during processing of PAXgene Tissue fixed, paraffin-embedded tissue (PFPE) on RNA yield, integrity, and performance in quantitative RT-PCR (EN)Show detailsInfluence of modifications to processing protocol on RNA yield and integrity (EN)Show details(EN) - Simultaneous perservation of RNA and morphology in tissue samples fixed with PAXgene Tissue Fix and stored for up to 2 years in PAXgene Tissue Stabilizer at –20°C or –80°CShow detailsVacuum sealing of fixed and stabilized tissue with a FoodSaver Vacuum Sealer for dry and safe transport (EN)Show detailsRNA stability in tissue samples fixed and stabilized with the PAXgene Tissue System for up to 7 days at 22°C or 2 months at 4°C (EN)Show detailsYield, purity, and integrity of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) rat tissue (EN)Show detailsShow allShow lessSafety Data Sheets (1) Sort optionsSort alphabetically MSDS PAXgene Tissue RNA/miRNA Kit (50)Show allShow less References 0 fragment fix placeholderCustomers who bought these products also boughtCat No./ID:767134PAXgene Tissue DNA Kit (50)For 50 DNA preps: PAXgene DNA Mini Spin Columns, Processing Tubes, Microcentrifuge Tubes, Carrier RNA, and Buffers; 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