Sample Collection & StabilizationDNA5RNA19Tissue/FFPE5RNAprotect Tissue ReagentPrint For immediate stabilization of RNA in tissuesImmediate RNA stabilization and protection, due to RNase inactivationReliable gene expression and gene-profiling dataConvenient and safe handling at room temperature instead of freezing a sampleNo need for liquid nitrogen or dry ice, ideal for sample collection in the fieldTissue archiving without risk of RNA degradationRNAprotect Tissue Reagent immediately stabilizes RNA in tissue samples to preserve the gene expression profile. It is a storage solution that permeates harvested tissues rapidly to avoid freezing samples in liquid nitrogen. RNAprotect Tissue Reagent is supplied in 50 ml or 250 ml bottles. For stabilization of DNA, RNA, and protein in tissue samples, Allprotect Tissue Reagent can be used.The following kits are compatible with the RNAprotect Tissue Reagent: RNeasy Plus Kits, RNeasy Kits, miRNeasy Kits, and AllPrep Kits.Buy ProductsProduct DetailsProduct ResourcesBuy ProductsCat No./ID:76104RNAprotect Tissue Reagent (50ml)$88.60Order Product50 ml RNAprotect Tissue Reagent for stabilization of RNA in 25 x 200 mg tissue samplesCat No./ID:76106RNAprotect Tissue Reagent (250ml)$369.00Order Product250 ml RNAprotect TissueReagent for stabilization of RNA in 125 x 200 mg tissue samplesThe RNAprotect Tissue Reagent is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.QIAGEN’s former RNA Stabilization Reagent, was manufactured and offered under license from Ambion, Inc., the owner of the RNAlater® trade mark. Following the expiration of that license and corresponding patents, QIAGEN’s new product name for the identical formulation is RNAprotect Tissue Reagent.Product Details Stable RNA in tissues: different temperatures and freeze–thaw cycles.Total RNA was isolated from tissue samples stored in RNAprotect Tissue Reagent. [A] Rat lung tissue was stored under the indicated conditions. RNA was analyzed on a formaldehyde agarose gel and northern blot (GAPDH-probed).[B]Stabilized mouse liver tissue was subjected to freeze–thaw cycles (-20°C/25°C). C: control tissues frozen in liquid nitrogen and stored at -80°C.RNAprotect Reagent prevents degradation of mRNA in tissues.RNA was isolated from fresh rat kidney samples after 0, 5, 10, 15, 30, and 60 minutes, using either standard procedures (Unstabilized) or RNeasy Protect Kits (Stabilized). The RNA isolated was analyzed by agarose gel electrophoresis and expression of GAPDH was examined using northern blot analysis. M: markers.PerformanceRNAprotect TissueReagent stabilizes and protects the RNA expression pattern of samples during harvest and storage (see figure "RNAprotect Reagent prevents degradation of mRNA in tissues"). Immediate protection with RNAprotect technology ensures that downstream analyses truly reflect the expression profile of the intact tissues. Samples can be archived without risk of RNA degradation, even after multiple freeze–thaw cycles. RNeasy purified RNA from RNAprotect stabilized samples stored under a wide variety of conditions is ideal for downstream processes such as northern blotting and RT-PCR.PrincipleOnce a biological sample is harvested, its RNA becomes extremely unstable. The innovative, aqueous RNAprotectTissue Reagent quickly permeates tissues resulting in immediate stabilization of the RNA and preservation of the RNA expression pattern. Gene induction or down-regulation triggered by sample manipulation is also prevented, allowing accurate analysis of gene expression.ProcedureFor RNA protection and sample storage, tissue samples are submerged in an appropriate volume of RNAprotect Tissue Reagent immediately after harvesting. RNAprotect Tissue Tubes provide premeasured volumes of RNAprotect Tissue Reagent in reclosable tubes for convenient handling and sample storage. The reagent preserves RNA for up to 1 day at 37°C, 7 days at 18–25°C, or 4 weeks at 2–8°C, allowing processing, transportation, storage, and shipping of samples without liquid nitrogen or dry ice. Alternatively, the samples can also be placed at –20°C or –80°C for archival storage.ApplicationsAnimal and human tissues stabilized in RNAprotect Tissue Reagent can be efficiently disrupted using QIAGEN instruments for sample disruption, and used with QIAGEN kits for:Total RNA purificationmiRNA purificationmRNA purificationProduct ResourcesYou are not authorized to download the resourceApplication Notes (1) Sort optionsSort alphabetically Optimal RNAprotect incubation and removal conditions prior to isolation of total RNA from stabilized cell sampleShow detailsShow allShow lessBrochures & Guides (3) Sort optionsSort alphabetically All insights start with the sample: Your comprehensive guide for isolating top-quality RNAShow details(EN) - Stabilization Solutions for Reliable Gene Expression AnalysisShow details(EN) - Analyzing Gene Expression and RegulationShow detailsShow allShow lessFAQs (13) Sort optionsSort alphabetically Can RNAprotect Tissue Reagent be used to store previously isolated RNA?FAQ ID -337ViewI accidentally added RNAprotect Tissue Reagent to my cells, and now the cells are difficult to pellet. What can I do?FAQ ID -364ViewHow long can I store tissues preserved in RNAprotect Tissue Reagent?FAQ ID-3750ViewWhat has to be done to an RNA sample before loading it onto an Agilent Bioanalyzer?FAQ ID -528ViewWill pancreas tissue swim on top of RNAprotect Tissue Reagent?FAQ ID -536ViewIs it possible to perform laser microdissection with tissues stabilized with RNAprotect Tissue reagent?FAQ ID -608ViewDo you have a kit for RNA isolation from any kind of sample type?FAQ ID -627ViewWhat is the recommended incubation time to stabilize tissue RNA in RNAprotect Tissue Reagent?FAQ ID -647ViewCan RNAprotect Tissue Reagent be used to stabilize tissue which has already been flash-frozen?FAQ ID -757ViewWhy are some of my RNAprotect-stabilized samples frozen at 0ºC while others are not?FAQ ID -758ViewCan I stabilize adipose tissue using RNAprotect Tissue Reagent prior to RNA isolation?FAQ ID -856ViewWhy is it not recommended to stabilize cells with RNAprotect Tissue Reagent?FAQ ID -941ViewDo you have a protocol for the preparation of tissues preserved in RNAprotect Tissue Reagent for pathological studies?FAQ ID -950ViewShow allShow lessKit Handbooks (1) Sort optionsSort alphabetically RNAprotect Handbook - (EN)RNAprotect Tissue Tubes - For collection of harvested animal tissues with immediate stabilization of the gene expression profile, and subsequent transport and storage; RNAprotect Tissue Reagent - For immediate stabilization of the gene expression profile in harvested animal tissuesShow detailsShow allShow lessSupplementary Protocols (3) Sort optionsSort alphabetically Purification of total RNA from tissues using the RNeasy® Plus 96 Kit and vacuum/spin technology - (EN)Show detailsPurification of total RNA from tissues using the RNeasy® Plus 96 Kit and spin technology - (EN)Show detailsMicrobiome stabilization using the RNAprotect Tissue ReagentShow detailsShow allShow lessQuick-Start Protocols (1) Sort optionsSort alphabetically RNAprotect Tissue Reagent, RNAprotect Tissue Tubes, and RNeasy Protect Kits (EN)Show detailsShow allShow lessUser-Developed Protocols (1) Sort optionsSort alphabetically Preparation of RNAprotect preserved tissues for histological studies - (EN)Show detailsShow allShow lessSafety Data Sheets (3) Sort optionsSort alphabetically MSDS RNAprotect Tissue Reagent (50ml)MSDS RNAprotect Tissue Reagent (250 ml)CofAShow allShow less References 0 fragment fix placeholderCustomers who bought these products also boughtCat No./ID:12462 QIAGEN Large-Construct Kit (10) 10 QIAGEN-tip 500, Reagents, Buffers, ATP-Dependent Exonuclease$454.00Add To CartCat No./ID:27106X4QIAprep Spin Miniprep Kit (1000)For 1000 high-purity plasmid minipreps: 1000 QIAprep 2.0 Spin Columns, Reagents, Buffers, Collection Tubes (2 ml)$1,360.00Add To CartCat No./ID:74904RNeasy Plant Mini Kit (50)50 RNeasy Mini Spin Columns, 50 QIAshredder Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers$402.00Add To CartCat No./ID:74104RNeasy Mini Kit (50)50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers$330.00Add To CartCat No./ID:79254RNase-Free DNase Set (50)1500 Kunitz units RNase-free DNase I, RNase-free Buffer RDD, and RNase-free water for 50 RNA minipreps$113.00Add To CartCat No./ID:217184miRNeasy Serum/Plasma Kit (50)For 50 total RNA preps: 50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml and 2 ml), QIAzol Lysis Reagent, Ce_miR-39_1 miScript Primer Assay, RNase-free Reagents and Buffers$489.00Add To CartCat No./ID:74134RNeasy Plus Mini Kit (50)For 50 minipreps: RNeasy Mini Spin Columns, gDNA Eliminator Spin Columns, Collection Tubes, RNase-Free Water and Buffers$374.00Add To CartCat No./ID:12143QIAGEN Plasmid Midi Kit (25)25 QIAGEN-tip 100, Reagents, Buffers$303.00Add To CartCat No./ID:74106RNeasy Mini Kit (250)250 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers$1,451.00Add To CartCat No./ID:30210Ni-NTA Agarose (25 ml)25 ml nickel-charged resin (max. pressure: 2.8 psi)$323.00Add To CartCat No./ID:51104QIAamp DNA Blood Mini Kit (50)For 50 DNA minipreps: 50 QIAamp Mini Spin Columns, QIAGEN Protease, Reagents, Buffers, Collection Tubes (2ml)$167.00Add To CartCat No./ID:27104QIAprep Spin Miniprep Kit (50)For 50 high-purity plasmid minipreps: 50 QIAprep 2.0 Spin Columns, Reagents, Buffers, Collection Tubes (2 ml)$96.90Add To CartCat No./ID:51306QIAamp DNA Mini Kit (250)For 250 DNA preps: 250 QIAamp Mini Spin Columns, QIAGEN Proteinase K, Reagents, Buffers, Collection Tubes (2 ml)$757.00Add To CartCat No./ID:74004RNeasy Micro Kit (50)50 RNeasy MinElute Spin Columns, Collection Tubes (1.5ml and 2ml), RNase-free DNase I, Carrier RNA, RNase-free Reagents and Buffers$522.00Add To CartCat No./ID:79306QIAzol Lysis Reagent (200ml)200 ml QIAzol Lysis Reagent$336.00Add To CartCat No./ID:69504 DNeasy Blood & Tissue Kit (50) 50 DNeasy Mini Spin Columns, Proteinase K, Buffers, Collection Tubes (2 ml)$171.00Add To CartCat No./ID:51106QIAamp DNA Blood Mini Kit (250)For 250 DNA minipreps: 250 QIAamp Mini Spin Columns, QIAGEN Protease, Reagents, Buffers, Collection Tubes (2 ml)$720.00Add To CartCat No./ID:28104QIAquick PCR Purification Kit (50)For purification of 50 PCR reactions: 50 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)$123.00Add To CartCat No./ID:204574Rotor-Gene Probe RT-PCR Kit (400)For 400 x 25 µl reactions: 3 x 1.7 ml 2x Rotor-Gene Probe RT-PCR Master Mix, 100 µl Rotor-Gene RT Mix, 2 x 2 ml RNase-Free Water$689.00Add To CartCat No./ID:79654QIAshredder (50)50 disposable cell-lysate homogenizers for use in nucleic acid minipreps, caps$93.30Add To Cart