Discovery & Translational ResearchCustom LNA Oligonucleotides2DNA & RNA Purification4ISH & Northern Blotting7Lab Essentials1Next-Generation Sequencing5PCR/qPCR/dPCR1Sample Collection & Stabilization0Type-it Fast SNP Probe PCR KitPrint For accurate and reliable SNP genotyping using TaqMan® or TaqMan® MGB probes Validated using TaqMan® SNP Genotyping Assays Automated allele calling and tight fluorescence clusters Suitable for difficult SNP loci or very low template amounts Up to 40% time savings due to fast cycling procedureThe Type-it Fast SNP Probe PCR Kit is based on highly specific HotStarTaq Plus DNA Polymerase and a newly developed buffer system, both of which enable reliable and clear allelic discrimination. The combination of all components provided in the master mix result in improved accuracy and highly specific probe binding. The Type-it Fast SNP Probe PCR Kit is validated using TaqMan® SNP Genotyping Assays and enables reproducible SNP genotyping — even with difficult SNP loci (e.g., GC rich) or low amounts of starting template.Buy ProductsProduct DetailsSpecificationsProduct ResourcesBuy ProductsCat No./ID:206045Type-it Fast SNP Probe PCR Kit (800)$766.00Order ProductFor 800 x 25 μl reactions: 6 x 1.7 ml of 2x Type-it Fast SNP Probe PCR Master Mix, 5x Q-Solution, RNase-Free WaterCat No./ID:206047Type-it Fast SNP Probe PCR Kit (4000)$3,110.00Order ProductFor 4000 x 25 μl reactions: 2 x 25 ml of 2x Type-it Fast SNP Probe PCR Master Mix, 5x Q-Solution, RNase-Free WaterThe Type-it Fast SNP Probe PCR Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.See trademarks.Product Details Reliable SNP genotyping with small amounts of template.Allelic discriminationwas performed with a panel of 80 different genomic DNAs (10 ng or 1 ng each in 5μl reactions) using either the [A] Type-it Fast SNP Probe PCR Kit or[B] a genotyping master mix from Supplier A. Even when using low amounts of template, the Type-it Fast SNP Probe PCR Master Mix outperformed the master mix from Supplier A and provided tight clustering of alleles and reliable results. PCR was performed with a TaqMan® SNP genotyping assay for rs 951134 (aryl-hydrocarbon receptor repressor gene) and 2 no template controls (NTCs) and analyzed on an Applied Biosystems7900HT instrument. Black: NTCs; blue: homozygous for T allele (FAM fluorescence); green: heterozygous samples; red: homozygous for A allele (VIC fluorescence). Data was analyzed with 98% confidence.Genotyping workflow.The Type-it Fast SNP Probe PCR Master Mix consists of highly specific HotStarTaq Plus Polymerase, which enables fast and easy reaction setup at room temperature and ensures highly specific amplification — even with low template amounts.Highly specific probe binding.Buffer systems from other suppliers used for SNP genotyping often show poorer separation of allele clusters due to nonspecific binding of the mismatch probe. The Type-it Fast SNP Probe Buffer System provides increased discrimination by narrowing the probe melting temperature window, thereby reducing nonspecific binding of the mismatch probe. Wide and clear separation of allele clusters is obtained.Successful automated allele calling.Average automated allele call rates for a panel of different DNAs were analyzed using 6 different TaqMan® MGB-based SNP genotyping assays and 1 ng of genomic DNA from each of the 60 samples. PCR was performed with the indicated products in 384-well plates in a 5 μl reaction volume. Allelic discrimination plate read was performed on an Applied Biosystems7900HT instrument. The Type-it Fast SNP Probe PCR Kit consistently resulted in the highest call rates and the lowest error rates.Mechanism of fast cycling during annealing.[A] The proprietary PCR buffer contains Q-Bond, a molecule that facilitates the reduction of the annealing step to 5 seconds. Q-Bond increases the affinity of Taq DNA polymerases for short single-stranded DNA fragments, reducing the time needed for successful primer annealing. [B] Without Q-Bond, the primer and polymerase bind sequentially to the template, increasing the duration of the annealing step.Performance The Type-it Fast SNP Probe PCR Kit consistently ensures highly accurate SNP genotyping.  Highly stringent and specific binding of the allele-specific probe  The Type-it Fast SNP Genotyping PCR Master Mix is based on highly specific HotStarTaq Plus DNA Polymerase and a newly developed SNP genotyping PCR buffer system, both of which enable highly specific probe binding and consistently strong fluorescent signals. Compared to other commercially available SNP genotyping master mix chemistries, wider and clearer separation of allele clusters is obtained (see figure "Highly specific probe binding"). High call rates even for low template amounts Even with challenging targets or difficult SNP loci, the Type-it Fast SNP Probe PCR Kit provides reliable SNP genotyping — well-separated allele clusters and tight clustering of identical alleles are achieved, resulting in high call rates  (see figure "Successful automated allele calling"). The Type-it Fast SNP Probe PCR Kit outperformed kits tested from other suppliers, ensuring tight clustering of alleles — even with 1 ng of template DNA (see figure "Reliable SNP genotyping with small amounts of template"). PrincipleThe Type-it Fast SNP Probe PCR Kit is available in a convenient master mix format consisting of highly specific HotStarTaq Plus Polymerase and a unique SNP genotyping buffer system designed for fast and efficient amplification.The unique kit components provide tight allele clustering and outstanding separation to ensure high call rates, enabling reproducible andaccurate results (see table). High specificity and sensitivityHotStarTaq Plus DNA Polymerase, provided in the master mix, which ensures highly specific amplification, even with low template amounts. HotStarTaq Plus DNA Polymerase is provided in an inactive state with no polymerase activity at ambient temperatures. This prevents the formation of misprimed products and primer dimers at low temperatures.Unique buffer systemThe Type-it Fast SNP PCR Buffer, also included in the master mix, is specifically designed for fast-cycling SNP genotyping using sequence specific 5"-nuclease probes. The unique composition of the Type-it Fast SNP PCR Bufferensures highly stringent and specific binding of the allele-specific probe (match probe). This is due to the altered melting behaviour of the probes resulting in a narrower probe melting temperature window. Based on the original QIAGEN PCR Buffer, thisinnovative formulation enables a high ratio of specific-to-nonspecific primer binding during the short annealing step of every PCR cycle. Owing to a uniquelybalanced combination of KCl and (NH4)2SO4, the PCR buffer provides stringent primer annealing conditions over a wider range of annealing temperatures and Mg2+ concentrations than conventional PCR buffers. Optimization of PCR by varying the annealing temperature or the Mg2+ concentration is dramatically reduced and often not required. Additives in the Type-it Fast SNP PCR buffer, such as Q-Solution, provide reaction conditions for amplification of difficult genomic regions and difficult SNP loci. Innovative Q-Bond technology (for fast cycling)allows SNP genotyping results to be achieved faster (see figure "Mechanism of fast cycling during annealing"). The master mix also contains ROX dye at a concentration compatible with SNP genotyping on all instruments from Applied Biosystems. However, ROX dye is also compatible with other instruments not requiring ROX as a passive reference dye (seetable). Kit features Kit contents Features 2x Master Mix format* Developed for SNP genotyping with TaqMan® MGB probes Suitable for use with all cyclers suitable for SNP genotyping HotStarTaq Plus DNA Polymerase Fast and easy reaction setup at room temperature Highly specific amplification, even with low template amounts Type-it Fast SNP Probe PCR Buffer Wide separation of genotype clustersTight allele clusters and high allele calling ratesIncreased specificity in probe bindingFast cycling due to Q-Bond Molecule Q-Solution Tighter clusters and higher signals in scatter plot analysis for highly GC-rich SNP lociCan further improve suboptimal allele calling * Includes ROX reference dye, dNTPs, and MgCl2.ProcedureThe Type-it Fast SNP Probe PCR Kit is functionally verified with commercially available SNP genotyping assays and is compatible with TaqMan® MGB Probes, as well as user-developed probe-based assays consisting of TaqMan® MGB, TaqMan®, or other dual-labeled probes. The kit includes streamlined, preoptimized protocols for fast and reliable analysis.Fast-cycling procedure for straightforward results The Type-it Fast SNP Probe PCR Master Mix provides reaction conditions for fast-cycling PCR using sequence-specific probes.The buffer includes proprietary Q-Bond Molecule, which allows short cycling times on standard cyclers and on fast cyclers with rapid ramping rates. The fast-cycling procedure increases throughput by reducing PCR run times by up to 40% (see figure "Genotyping workflow"). Convenient kit formatThe kit isprovided ina ready-to-use, preoptimized master mix formatfor greater convenience. Use of a master mix saves time, simplifies handling for reaction setup, and increases reproducibility by eliminating many possible sources of pipetting errors and contamination — pipetting stepsare minimizedand tedious calculations are eliminated. HotStarTaq Plus DNA Polymerase(included in the master mix) is activated by a 5-minute, 95°C incubation step, which can easily be incorporated into existing thermal cycling programs.Room-temperature reaction setup using the master mix is fast and easy.Suitable instruments The Type-it Fast SNP Probe PCR Kit is optimized for amplification of SNP PCR assays on any suitable standard and fast ramping cycler compatible with optical PCR plates that fit into real-time PCR instruments used for fluorescence plate read analysis (see table). Suitable instruments Cyclers Model Real-time PCR cyclers QIAGEN: Rotor-Gene QABI PRISM 7900 (all series) ABI StepOne and StepOne Plus Applied Biosystems 7500 (all series) Applied BiosystemsViiA 7 Bio-Rad: iCycler iQ Bio-Rad: CFX series Roche: LightCycler 480Roche: LightCycler Nano Agilent: Mx3000P and Mx3005P Standard cyclers All cyclers in standard-ramping modes (e.g., GeneAmp 9700)All cyclers in fast-ramping modes (e.g., GeneAmp 9800) ApplicationsThe Type-it Fast SNP Probe PCR Kit is used to detect SNPs using TaqMan® MGB probes and can be used in various fields of research, including: Typing of disease loci Biomarker discoverySpecificationsFeaturesSpecifications ApplicationsProbe-based SNP Genotyping Enzyme activity5"-> 3" Exonuclease activity MastermixYes Product useFunctionally validated and developed for reliable SNP Genotyping Reaction typePCR amplification Real-time or endpointBoth Sample/target typeGenomic DNA Sequence specific ProbeTaqMan® Genotyping Assays, TaqMan® or TaqMan® MGB probes With or without ROXROX included in Master Mix With/without hotstartWithProduct ResourcesYou are not authorized to download the resourceBrochures & Guides (3) Sort optionsSort alphabetically Analyzing Genetic Differences - (EN)Second edition — innovative toolsShow details(EN) - Rotor-Gene Q - Pure DetectionNow with even more applications!Show details(EN) - Maximizing PCR and RT-PCR success — Third EditionAddressing critical factors and new solutionsShow detailsShow allShow less (1) Sort optionsSort alphabetically Product Profile - Type-it Fast SNP Probe PCR KitShow detailsShow allShow lessFAQs (2) Sort optionsSort alphabetically Why is special Type-it Fast SNP Probe PCR chemistry required for TaqMan® SNP Genotyping?FAQ ID -2057ViewCan other probe technologies besides TaqMan® be used with the Type-it Fast SNP Probe PCR Kit?FAQ ID -2058ViewShow allShow lessKit Handbooks (1) Sort optionsSort alphabetically (EN) - Type-it Fast SNP Probe PCR HandbookShow detailsShow allShow lessTechnical Information (1) Sort optionsSort alphabetically (EN) - Maximizing end-point PCR success with QIAGEN"s automatable PCR solutionsShow detailsShow allShow lessSafety Data Sheets (2) Sort optionsSort alphabetically MSDS Type-it Fast SNP Probe PCR Kit (800)MSDS Type-it Fast SNP Probe PCR Kit (4000)Show allShow lessfragment fix placeholderCustomers who bought these products also boughtCat No./ID:929554 QX DNA Size Marker 50 bp–1.5 kb (100 ul); 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