Sample Collection & StabilizationDNA5RNA19Tissue/FFPE5QIAsymphony PAXgene Blood ccfDNA KitPrint For purification of ccfDNA from plasma using the QIAsymphony SP Optimized chemistry and input volumes for PAXgene Blood ccfDNA Tubes Separate protocol lines for small (STA) and large (LAF) ccfDNA fragments Prefilled reagent cartrdiges with bar code for safety and ease of use Standardized processing for high reproducibilityThe PAXgene Blood ccfDNA System consists of a blood collection tube (PAXgene Blood ccfDNA Tube) and a ccfDNA purification kit for theQIAsymphony (QIAsymphony PAXgene Blood ccfDNA Kit).Buy ProductsProduct DetailsSpecificationsProduct ResourcesBuy ProductsCat No./ID:768536QIAsymphony PAXgene Blood ccfDNA Kit (192)$4,060.00Order ProductReagent cartridges, accessories and proteinase K vials for 192 prepsFor Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.Product Details High performance, comparable to QIAamp Circulating Nucleic Acid Kit.Results from two studies assessing the performance of the QIAsymphony PAXgene Blood ccfDNA Kit. Study A compares ccfDNA yield and portion of double-stranded DNA of eluates generated with the QIAsymphony PAXgene Blood ccfDNA Kit to eluates from the manual QIAamp Circulating Nucleic AcidKit. Study B summarizes performance parameters for the PAXgene ccfDNA system. The QIAsymphony PAXgene Blood ccfDNA Kit provides consistently accurate results.PCR results from blood collected in PAXgene Blood ccfDNA Tubes remain stable over storage time.The change in delta-CT values of target versus control signal for ccfDNA isolated from whole blood stored in EDTA and PAXgene Blood ccfDNA Tubes was measured at different storage intervals. Shown are the relative signals of the 3 mutations covered by thetherascreen EGFR assay:[A] point mutation T790M,[B] deletion in exon 19 and[C] L858R. Each PCR was done in duplicate. To calculate the delta-CT value, the average CT of each mutant was subtracted from the average CT of the wildtype sub-assay. The average of all donors is shown in the figure for all tested time points. Thetherascreen EGFR assay defines a threshold for each sub-assays. Delta-CT values above this threshold result in false-negative calls. Results from the ccfDNA isolated from EDTA tubes exceeded this threshold over time whereas results from the PAXgene Blood ccfDNA Tubes remained stable.Two protocol lines of the QIAsymphony PAXgene Blood ccfDNA Kit for different research needs.With the standard protocols (STA), predominantly small ccfDNA fragments are isolated. The large fragment protocols (LAF) efficiently co-purify large and small ccfDNA fragments. Both protocol lines consist of one protocol for 2.4 ml and one protocol for 4.8 plasma input.The QIAsymphony SP processes samples containing magnetic particles.A magnetic rod protected by a rod cover enters a well containing the sample and attracts the magnetic particles. The magnetic rod cover is positioned above another well and the magnetic particles are released. The QIAsymphony SP uses a magnetic head with an array of 24 magnetic rods, and can therefore process up to 24 samples simultaneously. Collection, transfer and release of magnetic particles is repeated several times during sample processing.Isolated ccfDNA is compatible with methylation-specific downstream applications.Whole blood of 4 healthy donors was collected into either EDTA or PAXgene Blood ccfDNA Tubes. Blood was left at room temperature for up to 7 days. Each day, ccfDNA was isolated from samples, bisulphite converted (Epitect FAST kit, QIAGEN) and analyzed with the GEMINI assay (Clinical Genomics) based on 2 methylated (BCAT1 and IKZF1) and 1 control (ACTB) target. Three PCR replicates per target sequence were analyzed. ccfDNA from blood stored in EDTA tubes generated more false positives than from blood stored in PAXgene Blood ccfDNA Tubes (data courtesy of Clinical Genomics).PerformanceThe fully automated QIAsymphony PAXgene Blood ccfDNA Kit procedure generatesccfDNA yields comparable to the manual QIAamp Circulating Nucleic Acid Kit procedure (see figure High performance, comparable to QIAamp Circulating Nucleic Acid Kit). Since yield is strongly dependent of donor, individual yields may vary. Onboard measures of the QIAsymphony SP minimize cross-contamination.PrincipleApoptosis and lysis of white blood cells in unstabilized whole blood releases genomic DNA into plasma, which dilutes naturally occurring ccfDNA.PAXgene Blood ccfDNA Tubes contain an additive that prevents blood coagulation and immediately stabilizes blood cells via a non-crosslinking stabilization solution (see figure PCR results from blood collected in PAXgene Blood ccfDNA Tubes remain stable over storage time). This impedes the release of intracellular DNA and maintains constant ccfDNA levels in plasma. When used with PAXgene Blood ccfDNA Tubes, the proven magnetic particle technology of the QIAsymphony PAXgene Blood ccfDNA Kit provides a rapid, automated procedure to isolate ccfDNA of high quality and purity (see figure Isolated ccfDNA is compatible with methylation-specific downstream applications).ProcedureThe workflow begins with a centrifugation step that separates plasma from the cellular fraction of the whole blood collected into PAXgene Blood ccfDNA Tubes. The plasma is then carefully transferred into a second tube. Asecond centrifugation step can be carried out optionally to remove any traces of buffy coat. The pure plasma is then transferred to a third tube which is loaded into the QIAsymphony SP.In the QIAsymphony SP, plasma proteins are digested by proteinase K while ccfDNA binds to the surface of magnetic beads (see figure The QIAsymphony SP processes samples containing magnetic particles). Depending on the chosen protocol line, predominantly small ccfDNA fragments (STA protocol line)or small and large ccfDNA fragments (LAF protocol line)are isolated. Three wash steps ensurecontaminant removal. Finally, ccfDNA is eluted from the magnetic particles and is readyfor use in downstream applications (see figure Two protocol lines of the QIAsymphony PAXgene Blood ccfDNA Kit for different research needs).ApplicationsccfDNA purified using the QIAsymphony PAXgene Blood ccfDNA Kit and the QIAsymphony SP is ready for usein a wide range of downstream applications, including: PCR, digital, multiplex and quantitative real-time PCR Pharmacogenomic studies SNP genotyping Next generation sequencing Methylation assaysSpecificationsFeaturesSpecifications ApplicationsWide spectrum of research applications, including analysis of cancer tumor DNA and non-invasive prenatal testing Elution volume60 µl for the STA protocol line 60, 100 or 150 µl for the LAF protocol line FormatMagnetic beads Input volume2.4 ml or 4.8 ml plasma ProcessingManual: plasma centrifugation Automated: QIAsymphony SP Shelf life6 weeks for an open reagent cartridge Throughput192 samples per working day and instrument Time per run96 samples of 4.8 ml plasma in 5 h 40 minProduct ResourcesYou are not authorized to download the resourceFAQs (31) Sort optionsSort alphabetically Do I need to use a blood collection set with the PAXgene Blood ccfDNA Tube?FAQ ID - 3626ViewWhat is the shelf life of unused PAXgene Blood ccfDNA Tubes?FAQ ID - 3633ViewWhat is the expected ccfDNA yield from plasma generated from 10 ml of blood?FAQ ID - 3637ViewCan I use plasma from underfilled PAXgene Blood ccfDNA Tubes?FAQ ID - 3638ViewHow long can whole blood stabilized in PAXgene Blood ccfDNA Tubes be stored at room temperature?FAQ ID - 3639ViewCan whole blood in PAXgene Blood ccfDNA Tubes be stored at temperatures above 25°C?FAQ ID - 3640ViewCan whole blood in PAXgene Blood ccfDNA Tubes be stored at temperatures below 15°C?FAQ ID - 3641ViewCan whole blood be frozen in PAXgene Blood ccfDNA Tubes?FAQ ID - 3642ViewIs double centrifugation required for plasma processing?FAQ ID - 3643ViewHow long and at which temperature can plasma generated from whole blood in PAXgene Blood ccfDNA Tubes be stored?FAQ ID - 3644ViewHow should I thaw frozen plasma samples?FAQ ID - 3645ViewWhat should I do if cryoprecipitates form while thawing a frozen plasma sample?FAQ ID - 3646ViewWhich plasma volumes can I process with the QIAsymphony PAXgene Blood ccfDNA protocols?FAQ ID - 3648ViewHow many extractions can I perform with a QIAsymphony PAXgene Blood ccfDNA Kit, using the 2.4 ml or the 4.8 ml protocols?FAQ ID - 3649ViewHow long after first use can a reagent cartridge be stored for further use?FAQ ID - 3650ViewWhat is the shelf life of the QIAsymphony PAXgene Blood ccfDNA Kit?FAQ ID - 3651ViewFor what type of sample material is the QIAsymphony PAXgene Blood ccfDNA Kit intended?FAQ ID - 3652ViewWhich tubes are recommended for the QIAsymphony sample rackFAQ ID - 3653ViewWhich protocol line should I useto process plasma samples?FAQ ID - 3654ViewWhich elution volumes are available with the QIAsymphony PAXgene Blood ccfDNA Kit?FAQ ID - 3655ViewDoes the QIAsymphony PAXgene Blood ccfDNA Kit also allow eluting ccfDNA in less than 60 µl? FAQ ID - 3656ViewHow should eluates produced with the PAXgene Blood ccfDNA System be stored?FAQ ID - 3657ViewWhich downstream applications have been tested with ccfDNA purified from whole blood collected and processed with the PAXgene Blood ccfDNA System?FAQ ID - 3658ViewWhy is a blood collection set required to collect blood into the PAXgene Blood ccfDNA Tube?FAQ ID - 3628ViewCan reagent from the PAXgene Blood ccfDNA Tube flow back into the patient’s arm?FAQ ID - 3629ViewCan I use a different brand blood collection set with the PAXgene Blood ccfDNA Tube?FAQ ID - 3630ViewWhat is the blood draw volume of the PAXgene Blood ccfDNA TubeFAQ ID - 3631ViewWhat is the reagent in the PAXgene Blood ccfDNA Tube?FAQ ID - 3632ViewHow should unused PAXgene Blood ccfDNA Tubes be stored?FAQ ID - 3634ViewAre there special considerations for phlebotomy when using the PAXgene Blood ccfDNA Tube (blood collection set, discard tube, positioning of the tube, issues with short draws, etc.)?FAQ ID - 3635ViewAre PAXgene Blood ccfDNA Tubes sterile?FAQ ID - 3636ViewShow allShow lessBrochures & Guides (1) Sort optionsSort alphabetically Flyer: Primary Tube Handling with PAXgene Blood ccfDNA SystemShow detailsShow allShow lessScientific Posters (4) Sort optionsSort alphabetically A New Blood Collection Tube and Automated Extraction Method for Stabilization and Analysis of ccfDNAUllius et al., 2015Show detailsA Shortened, Automated Workflow for Processing Plasma Directly From Primary Blood Collection Tubes Reduces Turnaround Time and Risk of Sample Mix-up in ccfDNA Based ApplicationsVosset al., 2017Show detailsIntegrated Workflow for Blood Collection, ccfDNA Stabilization and Extraction for Non-invasive Prenatal TestingUllius et al., 2016Show detailsPerformance Evaluation of a New Integrated Blood Collection and Sample Preparation System for the Stabilization and Extraction of Circulating Cell-free DNA (ccfDNA)Ullius et al.Show detailsShow allShow lessApplication/Protocol Documents (1) Sort optionsSort alphabetically QIAsymphony Protocol Sheet PAXgene ccfDNA_2400 and ccfDNA_4800For purification ofccfDNA from human whole blood stabilized in PAXgene Blood ccfDNA Tubes using the QIAsymphony SP and QIAsymphony PAXgene Blood ccfDNA Kit.Show detailsShow allShow lessApplication Notes (1) Sort optionsSort alphabetically PAXgene Blood ccfDNA System Primary Tube Handling (EN)Show detailsShow allShow lessKit Handbooks (1) Sort optionsSort alphabetically QIAsymphony PAXgene Blood ccfDNA Kit HandbookFor purification of ccfDNA from plasma using the QIAsymphony SPShow detailsShow allShow lessLabware Files and Documents (1) Sort optionsSort alphabetically Labware List — QIAsymphony PAXgene Blood ccfDNA Kit (SOW-516-7)For use with software version 4.0 or higher Show detailsShow allShow lessSafety Data Sheets (1) Sort optionsSort alphabetically MSDS QIAsymphony PAXgene Blood ccfDNA Kit (192)Show allShow lessfragment fix placeholderCustomers who bought these products also boughtCat No./ID:180715QIAseq FX Single Cell DNA Library Kit (96)For 96 reactions: Buffers and reagents for cell lysis, whole genome amplification and library preparation including DNA fragmentation, end-repair and adapter ligation. 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