Sample Collection & StabilizationDNA5RNA19Tissue/FFPE5PAXgene Tissue DNA KitPrint For purification of DNA from tissues fixed and stabilized in PAXgene Tissue ContainersIntegrated fixation, stabilization, and purificationHigh-quality DNA from tissues Preserved tissue morphologyTissue samples fixed and stored in PAXgene Tissue Containers can be paraffin-embedded and used for pathological studies as well as for subsequent purification of DNA, RNA, and/or miRNA. The PAXgene Tissue DNA Kit provides purification of DNA from tissues fixed and stabilized in PAXgene Tissue Containers. Purification is carried out using silica-based DNA purification technology in a spin-column format. Used with the containers, the kit provides a complete preanalytical solution for collection, fixation, and stabilization through to purification of high-quality DNA for molecular analysis.The procedures can be fully automated on theQIAcube Connect.Buy ProductsProduct DetailsSpecificationsProduct ResourcesBuy ProductsCat No./ID:767134PAXgene Tissue DNA Kit (50)$296.00Order ProductFor 50 DNA preps: PAXgene DNA Mini Spin Columns, Processing Tubes, Microcentrifuge Tubes, Carrier RNA, and Buffers; to be used in conjunction with PAXgene Tissue ContainersSpecifications for fixation and storage conditions in PAXgene Tissue Fix and PAXgene Tissue Stabilizer were determined using animal tissues. For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.Product Details Significant hands-on time savings compared to the manual purification.Comparison of sample preparation times between manual and QIAcube procedures for 12 PAXgene Tissue-fixed, paraffin-embedded (PFPE) tissues. Hands-on time without incubation times.High-quality DNA from different tissue types fixed and stabilized in PAXgene Tissue Containers.Tissues from different organs were fixed for 3 hours in PAXgene Tissue Fix, transferred to PAXgene Tissue Stabilizer, and stored for 24 hours at room temperature. After processing, DNA was purified from sections of paraffin-embedded tissue using the PAXgene Tissue DNA Kit. M: markers.The PAXgene Tissue DNA procedure.High-quality DNA from PFPE tissue with preserved morphology.[A] Hematoxylin and eosin (H&E) staining of PAXgene Tissue-fixed, paraffin-embedded (PFPE) human colorectal cancer tissue and [B] DNA on agarose gel electrophoresis using 0.8% TBE buffered gels with 200 ng genomic DNA isolated in triplicate from 5 cases (#1–5) of human PFPE colorectral cancer. M: markers.PerformanceTotal DNA purified using the PAXgene Tissue DNA Kit is highly pure. DNA has A260/A280 ratios of 1.7–1.9, and absorbance scans show a symmetrical peak at 260 nm confirming the high purity of genomic DNA. Contamination is minimized, and purified DNA is ready to use in downstream applications with no detectable PCR inhibition. Together the container and kit provides a complete preanalytical solution for collection, fixation, and stabilization of tissue, and purification of high-quality DNA for molecular analysis (see figure "High-quality DNA from different tissue types fixed and stabilized in PAXgene Tissue Containers").Principle Current tissue fixation methods used in traditional histology are of limited use for molecular analysis. Fixatives that contain formaldehyde cross-link biomolecules and modify nucleic acids and proteins. During tissue fixation, storage, and processing, cross-links lead to degradation of nucleic acids. Since cross-links cannot be removed completely, the resulting chemical modifications can cause inhibition in sensitive downstream applications such as quantitative PCR or RT-PCR. In order to enable both molecular and traditional pathology testing from the same specimen, a method is needed for stabilization of molecular content and preservation of morphology. PreAnalytiX has developed the PAXgene Tissue System to meet such needs. The system consists of a tissue collection device (the PAXgene Tissue Container for collection, stabilization, storage, and transportation of human tissue specimens) and kits for purification of DNA, total RNA, or miRNA. PAXgene Tissue Containers provide tissue fixation for histopathology studies and enable purification of high-quality nucleic acids from the same sample for molecular analysis. The fixation and stabilization method preserves tissue morphology and the integrity of nucleic acids without destructive cross-linking and degradation found in formalin-fixed tissues. For isolation of genomic DNA, the system requires the use of PAXgene Tissue Containers for tissue collection and stabilization, followed by DNA isolation and purification using the PAXgene Tissue DNA Kit. Procedure PAXgene Tissue Containers are dual-chamber containers prefilled with 2 reagents. PAXgene Tissue Fix rapidly penetrates and fixes the tissue. After fixation, the tissue is removed from the PAXgene Tissue Fix and transferred to PAXgene Tissue Stabilizer in the second chamber of the same container. When the tissue is stored in PAXgene Tissue Stabilizer, nucleic acids and morphology of the tissue sample are stable for a minimum of 3 and a maximum of 7 days at room temperature or for up to 8 weeks at 2–8°C, depending on the type of tissue. Storage at –15 to –30°C is also possible for at least 26 months without any negative effects on the morphology of the tissue or the integrity of the nucleic acids. Stabilized samples can be embedded in paraffin for histological studies. Nucleic acids can be isolated from the PAXgene Tissue fixed, paraffin-embedded (PFPE) samples either before or after embedding in paraffin.  The PAXgene Tissue DNA Kit provides 3 protocols for purification of genomic DNA from tissues fixed and stabilized in PAXgene Tissue Containers. Lysis of the tissue sample is performed in the lysis buffer, Buffer TD1, with digestion using proteinase K. Buffering conditions are adjusted with binding Buffer TD2 and ethanol to provide optimal DNA-binding conditions, and the lysate is loaded onto the PAXgene DNA spin column. During centrifugation, DNA is selectively bound to the silica membrane, and contaminants pass through. Remaining contaminants and enzyme inhibitors are removed in 2 efficient wash steps with wash buffers TD3 and TD4, and DNA is then eluted in low-salt elution Buffer TD5, ready for use (see figure "The PAXgene Tissue DNA procedure"). ApplicationsThe purified DNA is ready to use in a wide range of downstream applications, including: PCR and quantitative, real-time PCR Southern blotting Pharmacogenomic studies SNP discovery and SNP genotyping SpecificationsFeaturesSpecifications ApplicationsPCR and quantitative, real-time PCR, Southern blotting, pharmacogenomic studies, SNP discovery and SNP genotyping Elution volume14–40 µl FormatSpin column Main sample typeHuman tissue ProcessingManual (centrifugation) Sample amount4 x 15 x 15 mm TechnologySilica technology Time per run30 min + 120 min incubation/8 samples YieldDepends on tissue type and starting material (fixed or PFPE*) * PAXgene Fixed Paraffin Embedded. Product ResourcesYou are not authorized to download the resourceBrochures & Guides (3) Sort optionsSort alphabetically PAXgene Tissue - (EN)Two worlds in one sampleShow detailsDNA Isolation with the PAXgene Tissue DNA Kit (EN)For isolation and purification of genomic DNA from tissue samples stabilized using the PAXgene Tissue SystemShow detailsPAXgene Tissue System Brochure (EN)Moving toward excellence and standardization in tissue collection and fixationShow detailsShow allShow lessFAQs (5) Sort optionsSort alphabetically Is a special processing protocol needed?FAQ ID -2523ViewIs it possible to microdissect PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissues?FAQ ID -2531ViewWhat is the purity of nucleic acids extracted with the PAXgene Tissue kits?FAQ ID -2533ViewWhat is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?FAQ ID -2538ViewWhere can I find additional information for PreAnalytiX PAXgene products? FAQ ID - 3515ViewShow allShow lessKit Handbooks (1) Sort optionsSort alphabetically (EN) - PAXgene Tissue DNA Kit HandbookFor isolation and purification of genomic DNA from tissue samples stabilized in PAXgene Tissue ContainersShow detailsShow allShow lessTechnical Information (1) Sort optionsSort alphabetically (EN) - Quantitative analysis of KRAS and BRAF mutational status in DNA from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue using Pyrosequencing technologyShow detailsShow allShow lessScientific Posters (6) Sort optionsSort alphabetically DNA Quality Measurement and Somatic Mutation Profiling in PAXgene Tissue Samples with qBiomarker Somatic Mutation PCR Arrays (EN)Long et al., ADAPT 2012Show detailsMorphological, Epigenomic, and Mutational Analyses of PAXgene Tissue Fixed, Paraffin-embedded (PFPE) Colorectal Cancer (CRC) Specimens (EN)Groelz et al.,AMP 2010Show detailsNew fixation technology for simlutaneous preservation of morphology and nucleic acids in tissue (EN)Groelz et al., AMP 2008Show detailsPreservation of morphology and biomolecules within tissue stored for three years at –80°C in PAXgene Tissue Stabilizer Reagent (EN)Groelz et al., ISBER 2012Show detailsPAXgene tissue fixation technology for simultaneous preservation of morphology and biomolecules (EN)Groelz et al., ECP 2012Show detailsPAXgene Tissue: A new fixation technology for simultaneous preservation of morphology and nucleic acids (EN)Groelz et al.,BRN Symposium 2011Show detailsShow allShow lessSupplementary Protocols (5) Sort optionsSort alphabetically Simultaneous purification of genomic DNA and total RNA, including miRNA, from sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue (EN)Show detailsSimultaneous purification of genomic DNA and total RNA from sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue (EN)Show detailsDeparaffinization of PAXgene® Tissue fixed, paraffin-embedded tissue sections with Deparaffinization Solution - (EN)Show detailsPurification of genomic DNA from microdissected PAXgene® Tissue-fixed, paraffin-embedded (PFPE) and PAXgene Tissue-fixed, cryo-embedded (PFCE) tissues - (EN)Show detailsPurification of genomic DNA from sections of PAXgene® Tissue fixed, cryo-embedded (PFCE) tissue placed directly into a microcentrifuge tube - (EN)Show detailsShow allShow lessSafety Data Sheets (1) Sort optionsSort alphabetically MSDS PAXgene Tissue DNA Kit (50)Show allShow less References 0 fragment fix placeholderCustomers who bought these products also boughtCat No./ID:766134PAXgene Tissue RNA/miRNA Kit (50)For 50 RNA preps: PAXgene RNA MinElute Spin Columns, PAXgene Shredder Spin Columns, Processing Tubes, Microcentrifuge Tubes, Carrier RNA, RNase-Free DNase, and RNase-Free Buffers; to be used in conjunction with PAXgene Tissue Containers$578.00Add To CartCat No./ID:765512PAXgene Tissue STABILIZER Concentrate (150 ml)8 bottles of PAXgene Tissue Stabilizer concentrate, for 4 liters of PAXgene Tissue STABILIZER$237.00Add To CartCat No./ID:765312PAXgene Tissue FIX Container (50 ml)For fixation and stabilization of tissue specimen: 10 prefilled Reagent Containers containing 50 ml of PAXgene Tissue FIX$171.00Add To CartCat No./ID:979202PyroMark Q24 Cartridge (3)Reusable dispensing tool for nucleotide and reagent delivery, for use with PyroMark Q24 and PyroMark Q24 Advanced$515.00Add To CartCat No./ID:979009PyroMark Annealing BufferFor annealing of sequencing primer to DNA template$58.80Add To CartCat No./ID:761133PAXgene Blood DNA Kit (25)Processing tubes and buffers for 25 preparations$366.00Add To CartCat No./ID:979006PyroMark Binding Buffer (200 ml)For binding of biotinylated PCR product to Sepharose beads$58.70Add To CartCat No./ID:59695EpiTect PCR Control DNA Set (100)Human control DNA set (containing both bisulfite converted methylated and unmethylated DNA and unconverted unmethylated DNA) for 100 control PCRs$432.00Add To CartCat No./ID:979008PyroMark Wash Buffer (conc., 200 ml)For use with the PyroMark Q24 Vacuum Workstation and PyroMark Q96 Vacuum Workstation to wash and neutralize the immobilized DNA$58.40Add To CartCat No./ID:939018Deparaffinization Solution(50 ml)1 x 50ml Deparaffinization Solution$349.00Add To CartCat No./ID:972804PyroMark Gold Q96 Reagents (5 x 96)For performing Pyrosequencing reactions on the PyroMark Q96 ID (5 x 96) and PyroMark Q96 MD (15 x 96)$601.00Add To CartCat No./ID:978703PyroMark PCR Kit (200)For 200 reactions: 2x PyroMark PCR Master Mix (includes HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer containing 3 mM MgCl2 and dNTPs), 10x CoralLoad Concentrate, 5x Q-Solution, 25 mM MgCl2, and RNase-Free Water.$321.00Add To CartCat No./ID:979007PyroMark Denaturation Sol. (500 ml)For denaturation of double-stranded PCR product intosingle-stranded DNA template$58.70Add To CartCat No./ID:1018013Buffer RPE (concentrate, 55 ml)55 ml concentrated wash buffer for use with RNeasy, miRNeasy, and AllPrep Kits; requires addition of ethanol$40.20Add To CartCat No./ID:79656QIAshredder (250)250 disposable cell-lysate homogenizers for use in nucleic acid minipreps, caps$406.00Add To CartCat No./ID:217004miRNeasy Mini Kit (50)For 50 preps: 50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), QIAzol Lysis Reagent, RNase-Free Reagents and Buffers$390.00Add To CartCat No./ID:79254RNase-Free DNase Set (50)1500 Kunitz units RNase-free DNase I, RNase-free Buffer RDD, and RNase-free water for 50 RNA minipreps$113.00Add To Cart