Sample Collection & StabilizationDNA5RNA19Tissue/FFPE5Allprotect Tissue ReagentPrint For immediate stabilization of DNA, RNA, and protein in tissuesNo need for liquid nitrogen or dry iceImmediate, convenient stabilization of harvested tissuesLong-term storage of tissues for later analysisStandardized sample preparation for systems biologyAllprotect Tissue Reagent provides immediate stabilization of DNA, RNA, and protein in tissue samples at room temperature. This preserves the in vivo profile of DNA, RNA, and proteins, allowing reliable downstream analysis. Stabilized tissues can be transported at 15–25°C for up to 7 days, or stored at 2–8°C for up to 12 months. For longer storage, stabilized tissues can be archived at –20°C or –80°C. Buy ProductsProduct DetailsProduct ResourcesBuy ProductsCat No./ID:76405Allprotect Tissue Reagent (100 ml)$684.00Order Product100 ml Allprotect Tissue Reagent, Allprotect Reagent PumpThe Allprotect Tissue Reagent is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.Product Details Intact protein for western blotting.Various rat tissues were stored in Allprotect Reagent at 25°C for 5 days prior to western blot analysis of ERK2. Blot intensities were similar to those from liquid nitrogen stabilized tissues, indicating no protein degradation.Prevention of gene induction.Rat lung was stored at 25°C in Allprotect Reagent or in PBS for the indicated times prior to real-time RT-PCR analysis of c-fos expression. Transcript levels relative to those of liquid nitrogen stabilized lung were calculated. The data show that Allprotect Reagent prevents c-fos induction.Prevention of protein degradation.Human intestinal mucosa samples were stored for 1 day in Allprotect Reagent (4°C) or in liquid nitrogen, and then analyzed by mass spectrometry. The Allprotect samples gave similar peak patterns for [A] <10 kDa and [B] 10–20 kDa protein as the liquid nitrogen samples, indicating stabilization of the protein content. Example analyses are shown. (Data kindly provided by Indivumed GmbH, Hamburg, Germany).Mechanism of Allprotect stabilization.Each Allprotector molecule possesses contact points that allow multiple Allprotector molecules to envelop and protect DNA, RNA, and protein. Upon tissue lysis, Allprotector molecules become diluted and fall apart, releasing DNA, RNA, and protein.Prevention of RNA degradation.Rat intestine was stored at 25°C in Allprotect Reagent or in PBS for the indicated times prior to real-time RT-PCR analysis of Madh7 expression. Transcript levels relative to those of liquid nitrogen stabilized intestine were calculated. The data show that Allprotect Reagent prevents RNA degradation.Preservation of protein activity.Rat liver was stored in Allprotect Reagent before total protein was purified using the Qproteome Mammalian Protein Prep Kit. Acidic phosphatase activity was similar to thatof protein from control liver stored in liquid nitrogen. Each bar shows the mean from two liver pieces assayed in duplicate.PerformanceImmediate stabilization of harvested tissues in Allprotect Tissue Reagent preserves the in vivo profile of DNA, RNA, and proteins. This enables reliable downstream analysis even with tissues stored at room temperature (15–25°C) for up to 7 days (see figures "Intact protein for western blotting", "Prevention of protein degradation", and "Preservation of protein activity"), Stabilization is especially critical for RNA, since transcript levels change rapidly after tissue harvesting (see figures "Prevention of gene induction" and "Prevention of RNA degradation"). Agilent 2100 Bioanalyzer analysis of RNA from Allprotect stabilized tissues gives high RIN values, indicating intact RNA. Allprotect Tissue Reagent is also able to stabilize molecules of increasing interest such as miRNA, methylated genomic DNA, and phosphorylated protein.PrincipleFreshly harvested tissue is submerged in Allprotect Tissue Reagent to immediately preserve the in vivo profile of DNA, RNA, and protein. Tissue samples in Allprotect Tissue Reagent are stabilized for long-term storage without freezing andcan be stored at room temperature for up to 7 days or at 2–8°C for up to12 months. The reagent is part of QIAGEN’s range of sample technologies for systems biology, and can be used in combination with the AllPrep DNA/RNA/Protein Mini Kit, which allows simultaneous purification of DNA, RNA, and protein from the same precious sample. Allprotect stabilized tissues are also compatible with QIAGEN kits for purification of DNA, RNA, or protein, such as DNeasy, QIAamp, RNeasy, and Qproteome Kits. Functional protein for enzyme assays can be purified from Allprotect stabilized tissues using the Qproteome Mammalian Protein Prep Kit. There is no need to split the sample into 3 prior to purification, allowing maximum recovery of DNA, RNA, and protein. In addition, no organic solvents such as phenol and acetone are required. DNA, RNA, and protein are all prepared from the same source, eliminating the variation inherent in preparing these analytes from different samples. QIAGEN sample technologies for individual purification of DNA, RNA, and protein are also available (see table). These include the Qproteome Mammalian Protein Prep Kit, which allows purification of functional protein from Allprotect stabilized tissues. Protein purified from Allprotect stabilized tissue with the Qproteome Mammalian Protein Prep Kit is suitable for all downstream applications. QIAGEN kits compatible with Allprotect Tissue Reagent Analytes purified Recommended kit Genomic DNA, total RNA, and total protein AllPrep DNA/RNA/Protein Mini Kit Genomic DNA and total RNA AllPrep DNA/RNA Micro Kit AllPrep DNA/RNA Mini Kit AllPrep DNA/RNA/miRNA Universal Kit Genomic DNA DNeasy Blood & Tissue Kit QIAamp DNA Mini Kit Total RNA RNeasy Plus Micro Kit* (for small tissue samples) RNeasy Mini Kit (for easy-to-lyse tissues) RNeasy Plus Mini Kit* (for easy-to-lyse tissues) RNeasy Fibrous Tissue Mini Kit (for fibrous tissues) RNeasy Lipid Tissue Mini Kit (for fatty tissues) RNeasy Microarray Tissue Mini Kit (for tissue samples for microarray analysis) Total protein Qproteome Mammalian Protein Prep Kit * Kit includes gDNA Eliminator columns Procedure Freshly harvested tissue is submerged in Allprotect Tissue Reagent to immediately stabilize DNA, RNA, and protein (see figure "Mechanism of Allprotect stabilization"). Stabilization takes place at room temperature, eliminating the use of hazardous liquid nitrogen or dry ice. Immediate stabilization of harvested tissues in Allprotect Tissue Reagent preserves the in vivo profile of DNA, RNA, and proteins, enabling reliable downstream analysis. Tissues stabilized in Allprotect Tissue Reagent can be stored at room temperature for up to 7 days or at 2–8°C for up to 12 months without showing any degradation of DNA, RNA, and protein. For longer storage, stabilized tissues can be archived at –20°C or –80°C. Applications DNA, RNA, and protein can be simultaneously purified from Allprotect stabilized tissues using the AllPrep DNA/RNA/Protein Mini Kit. The combination of Allprotect and AllPrep sample technologies standardizes sample preparation for systems biology. Genomic DNA and total RNA purified using the AllPrep DNA/RNA/Protein Mini Kit deliver optimal results in all downstream applications. These include real-time PCR and RT-PCR analyses. Protein purified from Allprotect stabilized tissue is denatured and suitable for SDS-PAGE and western blotting. Product ResourcesYou are not authorized to download the resourceBrochures & Guides (2) Sort optionsSort alphabetically (EN) - Analyzing Gene Expression and RegulationShow detailsAllprotect® Reagent and AllPrep® DNA/RNA/Protein KitShow detailsShow allShow lessFAQs (5) Sort optionsSort alphabetically Can tissue incubated in Allprotect Tissue Reagent be used for morphological studies?FAQ ID -1571ViewWhich homogenization method do you recommend for tissues stored in Allprotect Tissue Reagent?FAQ ID -1572ViewDoes residual Allprotect Tissue Reagent left behind on tissue samples interfere with DNA, RNA, and protein purification?FAQ ID -1573ViewCan Allprotect Tissue Reagent be used to stabilize RNA, DNA and proteins in cells, bacteria, yeast, and plants?FAQ ID -1574ViewCan samples in Allprotect Tissue Reagent undergo repeated freeze-thaws without degradation of DNA, RNA, and protein?FAQ ID -1575ViewShow allShow lessKit Handbooks (1) Sort optionsSort alphabetically (EN) - Allprotect Tissue Reagent HandbookFor immediate stabilization of DNA, RNA, and proteins in animal and human tissues, and subsequent storageShow detailsShow allShow lessScientific Posters (1) Sort optionsSort alphabetically (EN) - Stabilization of biomolecules in various sample materialsShow detailsShow allShow lessSupplementary Protocols (2) Sort optionsSort alphabetically Purification of total RNA from tissues using the RNeasy® Plus 96 Kit and vacuum/spin technology - (EN)Show detailsPurification of total RNA from tissues using the RNeasy® Plus 96 Kit and spin technology - (EN)Show detailsShow allShow lessSafety Data Sheets (2) Sort optionsSort alphabetically MSDS Allprotect Tissue Reagent (100 ml)CofAShow allShow less References 0 fragment fix placeholderCustomers who bought these products also boughtCat No./ID:37901Qproteome Mammalian Protein Prep KitFor approximately 100 protein preparations from cultured mammalian cells: Buffer, Reagents, Protease Inhibitor Solution, Benzonase$234.00Add To CartCat No./ID:80004AllPrep DNA/RNA/Protein Mini Kit (50)For 50 preps: AllPrep DNA Spin Columns, RNeasy Spin Columns, Collection Tubes, RNase-Free Reagents and Buffers$686.00Add To CartCat No./ID:951356MagAttract DNA Blood Midi M48 Kit (192)MagAttract Suspension B, Buffers, and RNase-Free Water for up to 192 x 350 µl preps$939.00Add To CartCat No./ID:979201PyroMark Q24 Plate (100)24-well plate for samples, for use with PyroMark Q24 and PyroMark Q24 Advanced; 100 plates in each package$249.00Add To CartCat No./ID:76106RNAprotect Tissue Reagent (250ml)250 ml RNAprotect TissueReagent for stabilization of RNA in 125 x 200 mg tissue samples$369.00Add To CartCat No./ID:59104EpiTect Bisulfite Kit (48)48 EpiTect Bisulfite Spin Columns, Reaction Mix, DNA Protect Buffer, Carrier RNA, Buffers$269.00Add To CartCat No./ID:1022064GFP-22 siRNA (5 nmol)Control siRNA targeting green fluorescent protein$182.00Add To CartCat No./ID:80224AllPrep DNA/RNA/miRNA Universal Kit (50)For 50 preps: 50 AllPrep DNA Mini Spin Columns, 50 RNeasy Mini Spin Columns, Collection Tubes, and Buffers$676.00Add To CartCat No./ID:74804RNeasy Lipid Tissue Mini Kit (50)50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), QIAzol Lysis Reagent, RNase-free Reagents and Buffers$425.00Add To CartCat No./ID:76204Buffer XBP (250 ml)250 ml Binding Buffer$84.90Add To CartCat No./ID:76214Buffer XE (45 ml)45 ml Elution Buffer for elution of intact extracellular vesicles$28.40Add To CartCat No./ID:80311AllPrep DNA/RNA 96 Kit (4)For 4 x 96 preps: 4 AllPrep 96 DNA Plates, 4 RNeasy 96 Plates, Elution Microtubes CL, Caps, S-Blocks, AirPore Tape Sheets, RNase-Free Reagents and Buffers$2,470.00Add To CartCat No./ID:51604QIAamp Fast DNA Stool Mini Kit (50)For 50 DNA preps: 50 QIAamp Mini Spin Columns, QIAGEN Proteinase K, InhibitEXBuffer, wash and elution buffers, Collection Tubes (2 ml)$285.00Add To Cart