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Qiagen/PAXgene Tissue FIX Container (50 ml)/For fixation and stabilization of tissue specimen: 10 prefilled Reagent Containers containing 50 ml of PAXgene Tissue FIX/765312
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Sample Collection & StabilizationDNA5RNA19Tissue/FFPE5PAXgene Tissue FIX Container (50 ml)Print For fixation and stabilization of tissues specimens Preservation of bothmorphology and biomolecules Fixation and paraffin embedding without formalin Improved molecular results from fixed tissues Tissue can be stored and archived for later processing RNA, miRNA, DNA, and/orproteins from one sample The PAXgene Tissue FIX Container (50 ml)enables fixation and stabilization of tissue specimens (up to 4 samples with a maximum size of 4 x 15 x 15 mm placed into 4 standard tissue cassettes, or a single tissue sample with a maximum size of 20 x 20 x 20 mm). PAXgene Tissue FIX Containers (50 ml)are single-chamber containers prefilled with 50 ml of PAXgene Tissue FIX. PAXgene Tissue FIX rapidly penetrates and fixes the tissue, preserving tissue morphology. Fixation is comparable to formalin fixation, but without the destructive nucleic acid crosslinking and degradation. After fixation, PAXgene Tissue FIX is removed and replaced by PAXgene Tissue STABILIZER within the same container. Nucleic acids, proteins, and morphology of the sample are stable up to 7 days at room temperature, and for longer periods at 2–8°C, or even at –20°C and –80°C. Stabilized samples can be embedded in paraffin for histological studies. PAXgene Tissue Kits and supplementary protocols provide efficient subsequent purification of RNA, miRNA, DNA, and/or proteins from the same sample.PAXgene Tissue FIX Container (50 ml)is only to be used in conjunction with PAXgene Tissue STABILIZER.Buy ProductsProduct DetailsProduct ResourcesBuy ProductsCat No./ID:765312PAXgene Tissue FIX Container (50 ml)$171.00Order ProductFor fixation and stabilization of tissue specimen: 10 prefilled Reagent Containers containing 50 ml of PAXgene Tissue FIXFor Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.Product Details Preservation of tissue morphology.Tissue was fixed and stabilized with the PAXgene Tissue FIX Container (50 ml); 4 µm sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue were stained with hematoxylin and eosin (HE). [A] Rat liver, [B] rat kidney, [C] rat intestine. Overview at 40x, details at 400x magnification.Absorbance ratio of high-quality, high molecular weight DNA from 12 different PAXgene Tissue-fixed, paraffin-embedded (PF) tissue types, processed on the QIAcube.Twelve different PAXgene Tissue-fixed (PF) rat tissue types, including fibrous or fatty, DNA-rich and DNA-poor tissue, processed with the PAXgene Tissue DNA Kit on the QIAcube in a single run. Ratio of absorbance at 260 and 280 nm.Comparison of manual and automated procedure: DNA yield from sections of PAXgene Tissue-fixed, paraffin-embedded (PFPE) tissue.DNA yield from 3 sections (thickness 10 µm) each of 8 different PAXgene Tissue-fixed, paraffin-embedded (PFPE) rat tissue types processed in triplicate with the PAXgene Tissue DNA Kit on the QIAcube or manually.Comparison of manual and automated procedure: Absorbance ratio from sections of PAXgene Tissue-fixed, paraffin-embedded (PFPE) tissue.Ratio of absorbance at 260 and 280 nm from 3 sections (thickness 10 µm) each of 8 different PAXgene Tissue-fixed, paraffin-embedded (PFPE) rat tissue types processed in triplicate with the PAXgene Tissue DNA Kit on the QIAcube or manually.DNA without chemical modification can be used for demanding downstream applications.Multiplex and long-range PCR of DNA from PAXgene Tissue-fixed, paraffin-embedded (PFPE) human colorectal cancer tissue (modified according to Viertler et al., (2012) A new technology for stabilization of biomolecules in tissues for combined histological and molecular analyses. J. MOl. Diagn. 14, 458). [A] Multiplex PCR of 8 different genomic DNA fragments ranging from 22 to 955 bp. [B] Long-range PCR of a 5 kb genomic DNA fragment.Protein extracted from PFPE tissue is suitable for two-dimentional gel electrophoresis.Non-malignant human duodenum tissue was divided into 3 samples and either flash-frozen in liquid nitrogen (cryo), or prepared as PFPE or FFPE tissue. Proteins from cryo and PFPE tissue were extracted in 2D buffer (30 mM Tris-HCl, pH 8.8, 7 M urea, 2 M thiourea, 4% CHAPS, 75 mM DTT) supplemented with protease inhibitor. Proteins from FFPE tissue were extracted in EXB Plus buffer supplemented with protease inhibitor, precipitated with acetone and resuspended in 2D buffer (as described in Guendisch et al. 2013). Samples (150 µg) were separated by 2-D PAGE. Data kindly provided by Karl-Friedrich Becker, Technical University of Munich, Germany.Agarose gel electrophoresis of high-quality, high molecular weight DNA from 12 different PAXgene Tissue-fixed (PF) tissue types, processed on the QIAcube.Twelve different PAXgene Tissue-fixed (PF) rat tissue types, including fibrous or fatty, DNA-rich and DNA-poor tissue, processed with the PAXgene Tissue DNA Kit on the QIAcube in one single run. DNA from each tissue type (300 ng) on agarose gel electrophoresis using 1% TBE buffered gels; M: markers.Comparison of manual and automated procedure: Agarose gel electrophoresis from sections of PAXgene Tissue-fixed, paraffin-embedded (PFPE) tissue.Agarose gel electrophoresis from 3 sections (thickness 10 µm) each of 8 different PAXgene Tissue-fixed, paraffin-embedded (PFPE) rat tissue types processed in triplicate with the PAXgene Tissue DNA Kit on the QIAcube and manually. DNA from each replicate and tissue type (200 ng) on agarose gel electrophoresis using 1% TBE buffered gels; M: markers.Detection of nondegraded, immunoreactive phosphoproteins from human clinical PFPE tissue.Non-malignant human uterus, breast, prostate and bladder tissue specimens were divided into 3 samples and either flash-frozen in liquid nitrogen (cryo), PAXgene Tissue-fixed, paraffin-embedded (PFPE) or formalin-fixed, paraffin-embedded (FFPE). Proteins from cryo, PFPE and FFPE tissues were extracted using the extraction buffer EXB Plus (Qproteome FFPE Tissue Kit; described in Ergin et al. 2010, Guendisch et al. 2013 and PAXgene Tissue supplementary protocols). SDS-PAGE and Western blot analysis were performed with 15 µg protein and the indicated antibodies. Data kindly provided by Karl-Friedrich Becker, Technical University of Munich, Germany.High concordance of miRNA expression between total RNA isolated from PFPE and fresh-frozen tissue.RNA, including miRNA, was purified from mirrored human breast cancer tissue fresh frozen in liquid nitrogen using the QIAGEN miRNeasy Kit, or from sections of PAXgene Tissue-fixed, paraffin-embedded (PFPE) tissue using the PAXgene Tissue RNA/miRNA Kit. Shown is a scatterplot of CT values from different single miRNA-specific RT-qPCR assays using the QIAGEN miScript System: miR9, -10a, -10b, -29a, -103, -125b, -143, -145, -192 and miScript PCR controls RNUA1, RNU5A, RNU6B, SNORD25, SCARNA19, SNORA73A; R2: coefficient of determination.RNA purified without chemical modification from PFPE tissue using the PAXgene Tissue RNA/miRNA Kit.SYBR Green real-time RT-qPCR was performed with 10 ng RNA from cryopreserved, formalin-fixed, paraffin-embedded (PPFE) or PAXgene Tissue-fixed, paraffin-embedded (PFPE) rat tissue (modified according to Groelz et al. 2013). Depicted are the average delta-CT values (delta-CT = CT[FFPE] – CT[cryo] or delta-CT = CT[PFPE] – CT[cryo]) from 6 different assays with amplicons ranging from 109 to 465 bp.Immunohistochemistry (IHC) staining with the PAXgene Tissue System gives comparable results to formalin-fixed tissue.Human palatine tonsil tissue was fixed in PAXgene Tissue FIX or with neutral-buffered formalin and embedded in paraffin. Primary antibodies to the indicated antigens were linked to a streptavidin-peroxidase conjugate by a biotinylated secondary antibody (LSAB method). Sectiosn were counterstained with hematoxylin. PFPE: PAXgene Tissue-fixed, paraffin-embedded. FFPE: formalin-fixed, paraffin-embedded.H&E staining with the PAXgene Tissue System gives results comparable to formalin-fixed tissue.Human tissue samples were divided into 2 sub-samples. One sub-sample was fixed with PAXgene Tissue FIX and the other was fixed with neutral-buffered formalin. The fixed tissues were embedded in paraffin, sectioned and stained with hematoxylin and eosin. PFPE: PAXgene Tissue-fixed, paraffin-embedded. FFPE: formalin-fixed, paraffin-embedded.The PAXgene Tissue FIX Container (50 ml) and PAXgene Tissue STABILIZER workflow.Single-chamber container for fixation and stabilization of a single, larger or multiple, smaller tissue samples.Yield of high-quality, high molecular weight DNA from 12 different PAXgene Tissue-fixed (PF) tissue types, processed on the QIAcube.Twelve different PAXgene Tissue-fixed (PF) rat tissue types, including fibrous or fatty, DNA-rich and DNA-poor tissues, processed with the PAXgene Tissue DNA Kit on the QIAcube in one single run. DNA yield per 10 mg tissue.PerformanceThe fixation and stabilization method preserves tissue morphology and the integrity of nucleic acids without destructive crosslinking and degradation found in formalin-fixed tissues (see figure "Preservation of tissue morphology").When fixed tissue is stored in PAXgene Tissue STABILIZER, the nucleic acids, proteins, and morphology of the tissue sample are stable for up to 7 days at room temperature (15–25°C) or for up to 4 weeks at 2–8°C. Tissue samples can even be stored in the PAXgene Tissue STABILIZER for longer periods at –20°C (–15°C to –30°C) or –80°C (–65°C to –90°C) without negative effects on the morphology of the tissue or the integrity of the nucleic acids. Specifications for fixation and storage conditions in PAXgene Tissue FIX and PAXgene Tissue STABILIZER were determined using animal tissues. PrincipleThe methods for tissue fixation currently used in traditional histology are of limited use for molecular analysis. Fixatives that contain formaldehyde crosslink biomolecules and modify nucleic acids and proteins. Such crosslinks lead to nucleic acid degradation during tissue fixation, storage, and processing. Since they cannot be removed completely, the resulting chemical modifications can cause inhibition in downstream applications, such as quantitative PCR or RT-PCR. To enable both molecular and traditional pathology testing from the same specimen, a method is needed to stabilize molecular content and preserve morphology. To meet this need, PreAnalytiX has developed the PAXgene Tissue System. The system consists of a fixation reagent (PAXgene Tissue FIX), a stabilization reagent (PAXgene Tissue STABILIZER), prefilled containers for tissue collection, storage, and transportation, and kits for purification of RNA, DNA, or total RNA, including miRNA. In addition, supplementary protocols for protein purification and other applications are available in the "Resources" section of this page or at www.preanalytix.com. PAXgene Tissue reagents in prefilled containers and PAXgene Tissue Kits provide a complete preanalytical solution for collection, fixation, and stabilization of tissue, and purification of high-quality nucleic acids for molecular research analysis.ProcedurePAXgene Tissue FIX Containers (50 ml)are single-chamber containers prefilled with 50 ml of PAXgene Tissue FIX. PAXgene Tissue FIX Containers (50 ml) can accommodate four standard tissue cassettes (not provided), which can hold tissue samples with a maximum size of 4 x 15 x 15 mm. PAXgene Tissue FIX Containers (50 ml) also offer the possibility for direct fixation of larger tissue samples with a maximum size of 20 x 20 x 20 mm. PAXgene Tissue FIX rapidly penetrates and fixes the tissue. After fixation for 2 to 48 hours, depending on tissue size, PAXgene Tissue FIX is removed and replaced by PAXgene Tissue STABILIZER within the same container. Stabilized samples can be embedded in paraffin for histological studies. Nucleic acids and proteins can be isolated from the stabilized samples before or after embedding in paraffin. See the PAXgene Tissue Kit Handbooks for information about DNA, RNA, or miRNA, and the PAXgene Tissue supplementary protocols in the "Resources" section of this page or at www.preanalytix.com for protein purification and other applications. ApplicationsSections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue can be used for histological studies or extraction of nucleic acids or proteins. Purification of RNA, total RNA, including miRNA, or DNA from PAXgene Tissue fixed and stabilized tissue samples requires the use of one of the PAXgene Tissue Kits for RNA, miRNA, or DNA. Purification of protein requires the Qproteome FFPE Kit.Product ResourcesYou are not authorized to download the resourceBrochures & Guides (5) Sort optionsSort alphabetically DNA Isolation with the PAXgene Tissue DNA Kit (EN)For isolation and purification of genomic DNA from tissue samples stabilized using the PAXgene Tissue SystemShow detailsHistological staining with the PAXgene Tissue System (EN)Show detailsProtein Extraction from PFPE - (EN)For extraction of full-length proteins from PFPE tissue using the Qproteome FFPE Tissue KitShow detailsRNA Isolation with the PAXgene Tissue RNA Kit (EN)For isolation and purification of intracellular RNA from tissue samples stabilized using the PAXgene Tissue SystemShow detailsPAXgene Tissue System Brochure (EN)Moving toward excellence and standardization in tissue collection and fixationShow detailsShow allShow lessFAQs (34) Sort optionsSort alphabetically What is the maximum tissue size that can be fixed in a PAXgene Tissue Container?FAQ ID -2518ViewHow long is the fixation time?FAQ ID -2519ViewIs a special processing protocol needed?FAQ ID -2523ViewIs it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue blocks?FAQ ID -2524ViewIs it possible to embed PAXgene Tissue fixed and stabilized samples in Optimal Cutting Temperature (OCT) medium for freezing?FAQ ID -2525ViewIs the morphology after H&E staining comparable to formalin-fixed samples?FAQ ID -2526ViewCan PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for in situ hybridization?FAQ ID -2529ViewCan sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for other histochemical staining techniques, such as PAS?FAQ ID -2530ViewIs it possible to microdissect PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissues?FAQ ID -2531ViewWhat is the purity of nucleic acids extracted with the PAXgene Tissue kits?FAQ ID -2533ViewWhat is the average RNA yield from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?FAQ ID -2534ViewWhat is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?FAQ ID -2538ViewAre PAXgene Tissue Containers suitable for long term-storage at freezing temperatures? FAQ ID -3030ViewIs it possible to use a standard processor — the kind used routinely for formalin-fixed samples — for dehydration and paraffin infiltration of PAXgene Tissue treated samples?FAQ ID -3032ViewWhich fixation method is used in the PAXgene Tissue System?FAQ ID - 3600ViewWhat is the fixation reagent of PAXgene Tissue FIX?FAQ ID - 3601ViewWhat is the stabilization reagent of PAXgene Tissue STABILIZER?FAQ ID - 3602ViewWhy are two reagents used in the PAXgene Tissue System?FAQ ID - 3603ViewWhat is the maximum tissue size that can be fixed in a PAXgene Tissue FIX Container (50 ml)?FAQ ID - 3604ViewWhich conditions are recommended for the storage of tissues in PAXgene Tissue STABILIZER?FAQ ID - 3605ViewIs it possible to process formalin-fixed and PAXgene Tissue fixed samples together in one run?FAQ ID - 3606ViewIs it necessary to clean a processor normally used for formalin-fixed tissue before using it with PAXgene Tissue fixed tissue?FAQ ID - 3607ViewIs it possible to integrate the PAXgene Tissue STABILIZER into tissue processing?FAQ ID - 3608ViewAre immunohistochemistry (IHC) assays developed for formalin-fixed, paraffin-embedded (FFPE) tissues compatible with PAXgene Tissue fixed, paraffin-embedded (PFPE) tissues?FAQ ID - 3609ViewIs it possible to use formalin-fixed, paraffin-embedded (FFPE) kits and protocols to isolate biomolecules from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?FAQ ID - 3610ViewHow well is RNA integrity preserved in PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?FAQ ID - 3611ViewHow well is DNA integrity preserved in PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?FAQ ID - 3612ViewWhat are the yield and integrity of nucleic acids isolated from blocks of PAXgene Tissue fixed, cryo-embedded (PFCE) tissue?FAQ ID - 3613ViewAre special kits and protocols required to isolate biomolecules from PAXgene Tissue fixed, cryo-embedded (PFCE) tissue? FAQ ID - 3614ViewDoes the PAXgene Tissue RNA Kit co-purify small RNAs?FAQ ID - 3615ViewCan proteins be extracted from PAXgene Tissue fixed specimens?FAQ ID - 3616ViewWhich kits and protocols can be used to isolate nucleic acids from microdissected PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) specimens?FAQ ID - 3617ViewWhat is the PCR performance of DNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissue compared to DNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?FAQ ID - 3618ViewHow is the quality of proteins purified from PAXgene Tissue fixed and stabilized tissue?FAQ ID - 3619ViewShow allShow lessKit Handbooks (2) Sort optionsSort alphabetically PAXgene Tissue FIX Container HandbookFor collection, fixation, and stabilization of multiple small tissue samples or a single large tissue sampleShow detailsPAXgene Tissue STABILIZER Reagent Product SheetFor stabilization of tissue specimens fixed in PAXgene Tissue FIX ContainerShow detailsShow allShow lessScientific Posters (14) Sort optionsSort alphabetically DNA Quality Measurement and Somatic Mutation Profiling in PAXgene Tissue Samples with qBiomarker Somatic Mutation PCR Arrays (EN)Long et al., ADAPT 2012Show detailsEvaluation of PAXgene Tissue System: preservation of morphology and gene expression in human melanoma (EN)Hesse et al., AACR-NCI-EORTC 2011Show detailsPreservation of gene expression profile and histomorphology in human breast tumor tissue with the new PAXgene Tissue System (EN)Groelz et al., AACR 2010Show detailsMorphological, Epigenomic, and Mutational Analyses of PAXgene Tissue Fixed, Paraffin-embedded (PFPE) Colorectal Cancer (CRC) Specimens (EN)Groelz et al.,AMP 2010Show detailsNew fixation technology for simlutaneous preservation of morphology and nucleic acids in tissue (EN)Groelz et al., AMP 2008Show detailsPreservation of histomorphology and nucleic acids in human breast tumor tissue with the new PAXgene Tissue System (EN)Groelz et al.,AMP 2009Show detailsPreservation of morphology and biomolecules within tissue stored for three years at –80°C in PAXgene Tissue Stabilizer Reagent (EN)Groelz et al., ISBER 2012Show detailsPAXgene vs. formalin fixed tissue: a comparison of tissue morphology and RNA quality (EN)Groelz et al.,BRN Symposium 2012Show detailsPAXgene tissue fixation technology for simultaneous preservation of morphology and biomolecules (EN)Groelz et al., ECP 2012Show detailsA new tissue fixative for biomarker discovery: Gene expression and miRNA in PFPE colorectal cancer (CRC) and breast cancer tissue vs. FFPE tissue (EN)Groelz et al.,3rd Annual Oncology Biomarkers 2011Show detailsRT-PCR performance of RNA obtained from archived FFPE and PFPE blocks of tissue (EN)Groelz et al., AMP 2014Show detailsPAXgene Tissue: A new fixation technology for simultaneous preservation of morphology and nucleic acids (EN)Groelz et al.,BRN Symposium 2011Show detailsStability of nucleic acids in archived formalin and PAXgene Tissue fixed, paraffin-embedded blocks of tissue (EN)Groelz et al., ECP 2014Show detailsLong-term storage of tissue specimens at –20­°C to –80°C with preservation of morphology and nucleic acids with frozen tissue (EN)Groelz et al.,AMP 2009Show detailsShow allShow lessSupplementary Protocols (16) Sort optionsSort alphabetically Simultaneous purification of genomic DNA and total RNA, including miRNA, from sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue (EN)Show detailsSimultaneous purification of genomic DNA and total RNA from sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue (EN)Show detailsDeparaffinization of PAXgene® Tissue fixed, paraffin-embedded tissue sections with Deparaffinization Solution - (EN)Show detailsManual processing of tissue specimens treated with the PAXgene® Tissue System - (EN)Show detailsCryo-embedding tissue specimens fixed and stabilized with the PAXgene® Tissue System - (EN)Show detailsPurification of genomic DNA from microdissected PAXgene® Tissue-fixed, paraffin-embedded (PFPE) and PAXgene Tissue-fixed, cryo-embedded (PFCE) tissues - (EN)Show detailsPurification of genomic DNA from sections of PAXgene® Tissue fixed, cryo-embedded (PFCE) tissue placed directly into a microcentrifuge tube - (EN)Show detailsPurification of total RNA from microdissected PAXgene® Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissues - (EN)Show detailsPurification of total RNA from sections of PAXgene® Tissue fixed, cryo-embedded (PFCE) tissue placed directly into a microcentrifuge tube - (EN)Show detailsPurification of total RNA, including miRNA, from microdissected PAXgene® Tissue fixed, paraffin embedded (PFPE) and PAXgene Tissue fixed, cryo-cryoembedded (PFCE) tissues - (EN)Show detailsPreparation of sections from PAXgene® Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissues for manual or laser microdissection (LMD) - (EN)Show detailsPreparation of PFPE tissue sections for use with in situ hybridization (ISH) staining assays - (EN)Show detailsPurification of total RNA, including miRNA, from sections of PAXgene® Tissue fixed, cryo-embedded (PFCE) tissue placed directly into a microcentrifuge tube - (EN)Show detailsPurification of full-length proteins from PAXgene® Tissue fixed and stabilized (PF) tissue samples - (EN)Show detailsPurification of full-length proteins from sections of PAXgene® Tissue fixed, cryo-embedded (PFCE) tissue - (EN)Show detailsPurification of full-length proteins from sections of PAXgene® Tissue fixed, paraffin-embedded (PFPE) tissue - (EN)Show detailsShow allShow lessTechnical Information (9) Sort optionsSort alphabetically Effect of epitope retrieval conditions on immunohistochemical staining of PFPE tonsil tissue with anti-human Ki-67 antigen (clone MIB-1) (EN)Show detailsMorphology and RNA preservation in PAXgene Tissue fixed, paraffin-embedded tissue (PFPE) stored for 18 months at different temperatures (EN)Show detailsInfluence of formalin contamination during processing of PAXgene Tissue fixed, paraffin-embedded tissue (PFPE) on RNA yield, integrity, and performance in quantitative RT-PCR (EN)Show detailsInfluence of modifications to processing protocol on RNA yield and integrity (EN)Show details(EN) - Simultaneous perservation of RNA and morphology in tissue samples fixed with PAXgene Tissue Fix and stored for up to 2 years in PAXgene Tissue Stabilizer at –20°C or –80°CShow detailsVacuum sealing of fixed and stabilized tissue with a FoodSaver Vacuum Sealer for dry and safe transport (EN)Show detailsQuantitative analysis of KRAS and BRAF mutational status in DNA from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue using Pyrosequencing technology (EN)Show detailsRNA stability in tissue samples fixed and stabilized with the PAXgene Tissue System for up to 7 days at 22°C or 2 months at 4°C (EN)Show detailsYield, purity, and integrity of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) rat tissue (EN)Show detailsShow allShow lessSafety Data Sheets (1) Sort optionsSort alphabetically MSDS PAXgene Tissue FIX Container (50 ml)Show allShow less References 0 fragment fix placeholderCustomers who bought these products also boughtCat No./ID:765512PAXgene Tissue STABILIZER Concentrate (150 ml)8 bottles of PAXgene Tissue Stabilizer concentrate, for 4 liters of PAXgene Tissue STABILIZER$237.00Add To CartCat No./ID:766134PAXgene Tissue RNA/miRNA Kit (50)For 50 RNA preps: PAXgene RNA MinElute Spin Columns, PAXgene Shredder Spin Columns, Processing Tubes, Microcentrifuge Tubes, Carrier RNA, RNase-Free DNase, and RNase-Free Buffers; to be used in conjunction with PAXgene Tissue Containers$578.00Add To CartCat No./ID:767134PAXgene Tissue DNA Kit (50)For 50 DNA preps: PAXgene DNA Mini Spin Columns, Processing Tubes, Microcentrifuge Tubes, Carrier RNA, and Buffers; to be used in conjunction with PAXgene Tissue Containers$296.00Add To CartCat No./ID:951356MagAttract DNA Blood Midi M48 Kit (192)MagAttract Suspension B, Buffers, and RNase-Free Water for up to 192 x 350 µl preps$939.00Add To CartCat No./ID:292035 mericon Chicken Kit (96) For96 reactions: PCR Assay Chicken, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-Free Water, 50x ROX Dye Solution$1,065.00Add To CartCat No./ID:13116-400Glass Beads, 0.5 mm (400 g)Customize your bead tubes with bulk beads for rapid and reliable biological sample lysis from a wide variety of starting materials.$264.00Add To CartCat No./ID:292065 mericon Sheep Kit (96)For 96 reactions: PCR Assay Sheep, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-Free Water, 50x ROX Dye Solution$1,070.00Add To CartCat No./ID:158924RNase A Solution (5 ml)5 ml RNase A Solution$446.00Add To CartCat No./ID:292015 mericon Pig Kit (96) For 96 reactions: PCR Assay Pig, Internal Control, Positive Control, Multiplex PCR Master Mix, QuantiTect Nucleic Acid Dilution Buffer, RNase-free water$1,057.00Add To CartCat No./ID:931447InvestigatorSTAR Lyse&PrepKit (400)For 400 preps of 300 µl each from casework and reference samples: Buffer ATL, Buffer QSL3, Buffer QSW1, Buffer QSW2, Bead Suspension G, Buffer ATE, Proteinase K, Carrier RNA, Q-Card, Handbook$1,826.00Add To CartCat No./ID:1027271miScript Inhibitor Neg. Control (5 nmol)5 nmol ready-to-transfect miRNA inhibitor for use as a negative control$313.00Add To CartCat No./ID:180415QIAseq 1-Step Amplicon Library Kit (96)For 96 reactions: 1-Step Amplicon Enzyme Mix, 4x 1-Step Amplicon Buffer, Adapter Plate 96-plex Illumina, Primer Mix Illumina Library Amp, HiFi PCR Master Mix, RNase-Free Water$2,997.00Add To CartCat No./ID:20902QIAEX II Suspension (1.5 ml)1.5 ml suspension$177.00Add To CartCat No./ID:20912Buffer QX1 (500 ml)500 ml Solubilization and Binding Buffer (with pH indicator)$107.00Add To CartCat No./ID:231222QIAGEN PCR Cloningplus Kit (10)For 10 reactions: 2x Ligation Master Mix (50 µl), pDrive Cloning Vector (0.5 µg), Distilled water (1.7 ml), QIAGEN EZ Competent Cells (10 tubes, 50 µl each), SOC medium (2 x 1.9 ml)$309.00Add To CartCat No./ID:74116RNeasy Mini QIAcube Kit (240)For 240 RNA minipreps: RNeasy Mini Rotor Adapters (preloaded with spin columns and elution tubes); RNase-free reagents and buffers$1,601.00Add To CartCat No./ID:57414QIAamp MinElute Media KitFor 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Proteinase K, Carrier RNA, Buffers, Extension Tubes (3 ml), Collection Tubes (1.5 ml)$361.00Add To Cart

QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业,总部位于德国。1984年,QIAGEN在德国成立,1996年在美国纽约纳斯达克上市。

QIAGEN拥有超过1000项专利和认可证明,在18个国家设立了分公司,代理商服务国超过40个,在全球有超过400000的用户。QIAGEN提供的产品超过500类,包括各种试剂,耗材和自动化纯化工作站。这些产品用于样品采集,稳定,核酸或蛋白的分离,纯化和检测中,不仅广泛的应用于科研领域的各个方面,在生物技术,制药,法医研究,食品安全检测,畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。


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