RNACell-Free RNA6Microbial RNA7miRNA10mRNA9RNA Clean Up3Total RNA31PAXgene Blood miRNA KitPrint For purification of miRNA and total RNA from whole blood Integrated system for collection, stabilization, and purification Effective purification of miRNA and total RNA RNA stabilization for up to 3 days at 18–25°CStabilization for at least 50 months at –20°C or –70°CThe PAXgene Blood miRNA System consists of the PAXgene Blood miRNA Kit for purification of total RNA, including RNA longer than approximately 18 nucleotides, and PAXgene Blood RNA Tubes (available from BD, cat. no. 762165) for collection, stabilization, and transport of blood. Purification is carried out using silica-based RNA purification technology in a spin-column format. Purification can be carried out manually, using a microcentrifuge, or automated on the QIAcube Connect. Used with the tubes, the kit provides a complete preanalytical solution for collection and stabilization through to purification of high-quality total RNA, including miRNA, for molecular analysis.Buy ProductsProduct DetailsSpecificationsProduct ResourcesBuy ProductsCat No./ID:763134PAXgene Blood miRNA Kit (50)$717.00Order ProductFor 50 RNA preps: PAXgene Spin Columns, PAXgene Shredder Spin Columns, Processing Tubes, Microcentrifuge Tubes, RNase-Free DNase, RNase-Free Reagents and Buffers; to be used in conjunction with PAXgene Blood RNA TubesFor Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.Product Details The QIAcube.Samples can be processed manually or automated on the QIAcube.High yields of small RNA species.Blood was collected in PAXgene Blood RNA Tubes and stored for 20–24 hours at room temperature before freezing at –15 to –30°C. RNA was purified using the PAXgene Blood RNA Kit (RNA kit) or the PAXgene Blood miRNA Kit (miRNA kit). [A] Purified RNA was analyzed by agarose gel electrophoresis. [B] Purified RNA was analyzed using the Agilent Small RNA LabChip. Small RNA species were significantly enriched when using the PAXgene Blood miRNA Kit.Analysis of miRNA species.Blood was collected in PAXgene Blood RNA Tubes and stored for 20–24 hours at room temperature before freezing at –15 to–30°C. RNA was purified using the PAXgene Blood RNA Kit or the PAXgene Blood miRNA Kit, as indicated. miRNA species were analyzed using the QIAGEN miScript PCR System, with the indicated miScript Primer Assays. The lower CT values for RNA purified using the PAXgene Blood miRNA Kit indicate higher amounts of those miRNA species.PerformanceBlood samples collected in PAXgene Blood RNA Tubes can be safely stored or transported at 15–25°C for up to 72 hours, at 2–8°C for up to 5 days, or at –20°C or –70°C for at  least 50 months without showing any significant RNA degradation or changes in transcript levels. Total RNA >18 nucleotides is purified from the stabilized blood samples with the PAXgene Blood miRNA Kit and includes both mRNA and small RNAs such as miRNA (see figure "High yields of small RNA species"). The miRNA species can be analyzed using the QIAGEN miScript PCR System (see figure "Analysis of miRNA species") or other real-time RT-PCR systems.Principle Blood samples are collected in PAXgene Blood RNA Tubes. The tubes contain a reagent that lyses blood cells and immediately stabilizes intracellular RNA to preserve the gene expression profile. RNA stabilization is critical for reliable downstream gene expression analysis. Without stabilization, degradation of RNA and upregulation or downregulation of transcripts occur immediately after blood is drawn. ProcedureWith the PAXgene Blood miRNA Kit, total RNA >18 nucleotides (including miRNA) is purified from the stabilized blood samples using well-established PAXgene silica-membrane technology. Purification can be carried out in a manual format or it is automatable on the QIAcube (see figure "The QIAcube").Applications The purified total RNA is ready to use and is ideally suited for any downstream application, including: RT-PCR and real-time RT-PCR Differential display cDNA synthesis Northern, dot, and slot blot analyses Primer extension Poly A+ RNA selection RNase/S1 nuclease protection Microarray analysis SpecificationsFeaturesSpecifications ApplicationsRT-PCR and real-time RT-PCR, differential display, cDNA synthesis, northern, dot, and slot blot analyses, primer extension, microarray analysis Elution volume40 µl FormatSpin column Main sample typeWhole blood ProcessingManual (centrifugation) or on QIAcube Sample amount2.5 ml TechnologySilica technology Time per run or per prep90 minutes/12 samples Yield>3 µg (total RNA)Product ResourcesYou are not authorized to download the resourceBrochures & Guides (3) Sort optionsSort alphabetically All insights start with the sample: Your comprehensive guide for isolating top-quality RNAShow detailsPAXgene Blood miRNA Kit Brochure (EN)For purification of intracellular RNA, including miRNA, from whole bloodShow detailsPAXgene Blood RNA System Brochure (EN)For collection, transport, and storageof whole blood and stabilization and purification of intracellular RNAShow detailsShow allShow lessFAQs (12) Sort optionsSort alphabetically What can I use to isolate RNA smaller than 200 nucleotides?FAQ ID -115ViewCan the PAXgene Blood miRNA System be used for diagnostic or prognostic procedures?FAQ ID -2494ViewHow great is the enrichment of miRNA when using the dedicated PAXgene Blood miRNA Kit compared to the PAXgene Blood RNA Kit?FAQ ID -2495ViewHow many samples can be processed with the PAXgene Blood miRNA Kit?FAQ ID - 3477ViewWhich sample types can be processed using the PAXgene Blood miRNA protocols? FAQ ID - 3478ViewDo the samples require pretreatment before starting the manual protocol or before loading onto the QIAcube instrument? FAQ ID - 3479ViewDo you have stability data for RNA isolated with PAXgene miRNA Kit?FAQ ID - 3480ViewWhat are the expected yields of RNA from PAXgene Blood RNA Tubes isolated with the PAXgene Blood miRNA kit?FAQ ID - 3481ViewWhat is the maximum number of samples for the QIAcube variant of the PAXgene Blood miRNA protocol for a single run?FAQ ID - 3482ViewIs it possible to isolate genomic DNA or viral nucleic acids with PAXgene Blood miRNA protocol?FAQ ID - 3483ViewWhere can I find additional information for PreAnalytiX PAXgene products? FAQ ID - 3515ViewDo eluates generated with the PAXgene Blood miRNA Kit contain residual genomic DNA?FAQ ID - 3637ViewShow allShow lessScientific Posters (3) Sort optionsSort alphabetically Isolation of miRNAs and Other Small RNA Species from PAXgene Blood RNA Tubes (EN)Voss et al.,AMP 2009Show detailsIsolation of Small RNA Species from PAXgene Blood RNA Tubes (EN)Voss et al.,qPCR 2009Show detailsEstablishment of miRNA Profiles from Human Whole Blood in Combination with Other Genetic Data (EN)Kruhoffer et al., AMP 2007Show detailsShow allShow lessApplication Notes (2) Sort optionsSort alphabetically PAXgene Blood miRNA System versus Tempus Technical NoteComparison of miRNA recovery from the PAXgene Blood miRNA System and the Tempus Blood RNA Tube processed with the Tempus Spin RNA Isolation KitShow detailsPAXgene Blood miRNA System Technical NoteTypical total RNA yields from PAXgene Blood RNA Tubes processed with the PAXgene Blood miRNA KitShow detailsShow allShow lessKit Handbooks (1) Sort optionsSort alphabetically PAXgene Blood miRNA Kit HandbookFor purification of miRNA and total RNA from whole blood.Show detailsShow allShow lessSafety Data Sheets (2) Sort optionsSort alphabetically MSDS PAXgene Blood miRNA Kit (50)CofAShow allShow lessfragment fix placeholderProduct citations in scientific articlesPowered by Bioz See more details on BiozCustomers who bought these products also boughtCat No./ID:762331PAXgene 96 Blood RNA Kit (4)4 PAXgene 96 RNA Plates, 4 PAXgene 96 Filter Plates, Buffers, Proteinase K, RNase-free DNase Sets, AirPore Tape Sheets, Collection Vessels. To be used in conjunction with PAXgene Blood RNA Tubes.$3,870.00Add To CartCat No./ID:51185QIAamp DNA Blood Midi Kit (100)For 100 DNA midipreps: 100 QIAamp Midi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (15ml)$977.00Add To CartCat No./ID:951356MagAttract DNA Blood Midi M48 Kit (192)MagAttract Suspension B, Buffers, and RNase-Free Water for up to 192 x 350 µl preps$939.00Add To CartCat No./ID:36113Ni-NTA Magnetic Agarose Beads (6 x 1 ml)6 x 1 ml nickel-charged magnetic agarose beads (5%suspension)$703.00Add To CartCat No./ID:761133PAXgene Blood DNA Kit (25)Processing tubes and buffers for 25 preparations$366.00Add To CartCat No./ID:990399Spin Column Adapter Rings (25)Spin column adapter rings for use with spin columns that have screw caps; not required for all protocols$149.00Add To CartCat No./ID:762164PAXgene Blood RNA Kit (50)50 PAXgene Spin Columns, 50 PAXgene Shredder Spin Columns, Processing Tubes, RNase-Free DNase I, RNase-Free Reagents and Buffers. To be used in conjunction with PAXgene Blood RNA Tubes$588.00Add To CartCat No./ID:217184miRNeasy Serum/Plasma Kit (50)For 50 total RNA preps: 50 RNeasy MinElute Spin Columns, Collection Tubes (1.5 ml and 2 ml), QIAzol Lysis Reagent, Ce_miR-39_1 miScript Primer Assay, RNase-free Reagents and Buffers$489.00Add To CartCat No./ID:51194QIAamp DNA Blood Maxi Kit (50)For 50 DNA maxipreps: 50 QIAamp Maxi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (50 ml)$990.00Add To CartCat No./ID:9014579QIAvac MultiwellVacuum manifold for processing 96-well purification plates of SBS standard$888.00Add To CartCat No./ID:80224AllPrep DNA/RNA/miRNA Universal Kit (50)For 50 preps: 50 AllPrep DNA Mini Spin Columns, 50 RNeasy Mini Spin Columns, Collection Tubes, and Buffers$676.00Add To CartCat No./ID:764133PAXgene Bone Marrow RNA Kit (30)For 30 RNA preps: 30 PAXgene Spin Columns, 30PAXgene Shredder Spin Columns, Processing Tubes, RNase-free DNase I, RNase-free reagents and buffers. To be used in conjunction with PAXgene Bone Marrow RNA Tubes$707.00Add To CartCat No./ID:34924Polypropylene Columns (1 ml)50/pack, 1 ml capacity$147.00Add To CartCat No./ID:32915N-Terminus pQE Vector Set25 µg each: pQE-9, pQE-30, pQE-31, pQE-32, pQE-40$718.00Add To CartCat No./ID:59826 EpiTect Fast DNA Bisulfite Kit (200) For 200 preps: Bisulfite Solution, DNA Protect Buffer, MinElute DNA Spin Columns, Carrier RNA, and Buffers$785.00Add To CartCat No./ID:1022064GFP-22 siRNA (5 nmol)Control siRNA targeting green fluorescent protein$182.00Add To CartCat No./ID:76603miRCURY Exosome Serum/Plasma KitFor isolation of exosomes and other extracellular vesicles from serum or plasma; includes 10 ml Precipitation Buffer A, 10 ml Resuspension Buffer, 200 U Thrombin and 500 µl Thrombin Buffer$335.00Add To CartCat No./ID:217504miRNeasy FFPE Kit (50)50 RNeasy MinElute Spin Columns, Collection Tubes, Proteinase K, RNase-Free DNase I, DNase Booster Buffer, RNase-Free Buffers, RNase-Free Water$460.00Add To Cart