Sample Collection & StabilizationDNA5RNA19Tissue/FFPE5QIAamp Virus BioRobot MDx KitPrint For automated purification of viral DNA and RNA from cell-free body fluids on the BioRobot MDx workstationRapid isolation of high-quality, ready-to-use viral DNA and RNANo organic extraction or alcohol precipitationConsistent, high yieldsRemoval of contaminants and inhibitors The QIAamp Virus BioRobot MDx Kit provides automated viral nucleic acid purification on the BioRobot MDx using proven QIAamp technology. The fully automated procedure requires less than 2.5 hours, including bar code reading and complete process documentation, with no hands-on time during the run. Buy ProductsProduct DetailsSpecificationsProduct ResourcesBuy ProductsCat No./ID:965652QIAamp Virus BioRobot MDx Kit (12)$4,773.00Order ProductFor 12 x 96 preps for use on the BioRobot MDx; includes: 12 QIAamp 96 Plates, RNase-free buffers, QIAGEN Protease, Elution Microtubes CL, caps, S-Blocks, carrier RNAThe QIAamp Virus BioRobot MDx Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.See trademarks.Product Details QIAamp Virus BioRobot MDx protocol.PerformanceThe QIAamp Virus BioRobot MDx process provides uniform DNA and RNArecovery and purity across the 96-well plate with no detectablecross-contamination between samples. Up to 265µl of cell-free body fluids can be processed. The typical elution volumeis75 µl. Viruses can be detected with high sensitivity when using this kit, which is illustrated by hit-rate studies (see table). Further advantages of the system include high process reliability and walkaway automation, providing a highly efficient system for purification of viral nucleic acids. The QIAamp Virus BioRobot MDx provides reliable results in downstream applications. High precision using the QIAamp Virus BioRobot MDx Kit PCR product (n=96) Instrument 1 Instrument 2 Mean CT Standard deviation %CV Mean CT Standard deviation %CV Mimic virus 28.26 0.10 0.35 28.38 0.18 0.65 Internal control 31.98 0.16 0.51 31.87 0.22 0.70 Plasma was spiked with armored RNA molecules to mimic RNA viruses (25,000 copies per prep) and processed using the QIAamp Virus BioRobot MDx protocol in replicates of 96 on two separate instruments. An internal control RNA was added to the lysis buffer. The eluted RNA was amplified using a TaqMan®RT-PCR system. Mean CT value, standard deviation, and %CV were calculated for both the mimic virus and the internal control. (Data generated by QIAGEN R&D department.)Principle The QIAamp Virus BioRobot MDx Kit simplifies isolation of viral RNA and DNA from cell-free body fluids with a fast automated 96-well–plate procedure. No phenol–chloroform extraction is required. Nucleic acids bind specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acids to be eluted in either water or a buffer provided with the kit. QIAamp technology yields viral RNA and DNA from cell-free body fluids ready to use in PCR and blotting procedures. The fully automated procedure, including bar code reading and complete process documentation, requires less than 2.5 hours to process 96 samples (including a detailed load check of 20 minutes) with no hands-on time during the run. Advantages of the system include high process reliability and walkaway automation, providing a highly efficient system for purification of viral nucleic acids for laboratories. QIAamp sample preparation technology is fully licensed. ProcedureOptimized buffers lyse samples, stabilize nucleic acids, and enhance selective nucleic acid adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp 96-well plate. Wash buffers are used to remove impurities and pure, ready-to-use nucleic acids are then eluted in water or low-salt buffer (see flowchart "Protocol").Applications The QIAamp Virus BioRobot MDx Kit provides automated, high-throughput purification of viral RNA and DNA on the BioRobot MDx using proven QIAamp technology. The 96-well plates provide the flexibility of processing 32–96 samples, in increments of 8. Sample sources include: Plasma and serum CSF Other cell-free body fluids Cell-culture supernatants SpecificationsFeaturesSpecifications ApplicationsPCR, qPCR, real-time PCR Elution volume75 µl For automated processingBioRobot MDx Workstation Format96-well plates Main sample typeSerum, plasma ProcessingAutomated Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinViral DNA, viral RNA Sample amount265 µl Samples per run (throughput)36-92 samples per run TechnologySilica technology Time per run or per prep Yield>90% recoveryProduct ResourcesYou are not authorized to download the resourceFAQs (3) Sort optionsSort alphabetically How do I safely inactivate biohazardous flow-through material?FAQ ID -12ViewWhat can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?FAQ ID -728ViewWhat is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits? FAQ ID -761ViewShow allShow lessKit Handbooks (1) Sort optionsSort alphabetically QIAamp Virus BioRobot MDx Kit Handbook - (EN)For simultaneous purification of viral RNA and DNA from plasma, serum, cell-culture supernatants, and cell–free body fluids using the BioRobot MDxShow detailsShow allShow lessBrochures & Guides (1) Sort optionsSort alphabetically Product Profile - QIAamp® virus kitsShow detailsShow allShow lessSafety Data Sheets (2) Sort optionsSort alphabetically MSDS QIAamp Virus BioRobot MDx Kit (12)CofAShow allShow lessfragment fix placeholderCustomers who bought these products also boughtCat No./ID:1067924Buffer AW1 (151 ml)Buffer AW1 (151 ml)$106.00Add To CartCat No./ID:967852RNeasy 96 Universal Tissue 8000 Kit (12)For 12 x 96 total RNA preps on the BioRobot 8000 or BioRobot Universal System: 12 RNeasy 96 Plates, Collection Microtubes, Elution Microtubes CL, Caps, S-Blocks, QIAzol Lysis Reagent, RNase-Free Reagents and Buffers$5,558.00Add To CartCat No./ID:9012598Disposable Filter-Tips, 1100 µl (960)Conducting disposable filter-tips; pack of 960$273.00Add To CartCat No./ID:9012595Disposable Tips, 1100 µl (960)Conducting disposable tips; pack of 960$151.00Add To CartCat No./ID:19073Buffer AVL (155 ml)For 250 preps: 155 ml Viral Lysis Buffer, 1550 µg Carrier RNA$164.00Add To CartCat No./ID:9232764Disposable Troughs, 30 ml (10) Nonsterile, plastic, disposable troughs for PAXgene 96 procedures on the BioRobot MDx workstation, 10 troughs$15.60Add To CartCat No./ID:19081Buffer AW1 (concentrate, 242 ml)242 ml Wash Buffer (1) Concentrate for 1000 spin, 250 midi, or 100 maxi preps$106.00Add To CartCat No./ID:990610Filter-Tips, OnCor C, 200 µl (10 x 96)Disposable filter-tips; for use with QIAcube HT and QIAxtractor$169.00Add To CartCat No./ID:965842QIAamp 96 DNA Swab BioRobot Kit (12)For 12 x 96 DNA preps: 12 QIAamp 96 Plates, Buffers, QIAGEN Proteinase K, AirPore Tape Sheets, Tape Pad, S-Blocks, Racks with Collection Microtubes (1.2 ml), Caps$4,188.00Add To CartCat No./ID:965672QIAamp One-For-All Nucleic Acid Kit (12)For use on the BioRobot MDx. 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