Sample Collection & StabilizationDNA5RNA19Tissue/FFPE5RNeasy Protect Animal Blood SystemPrint For collecting and stabilizing animal blood and purifying RNA and miRNA Reliable stabilization of transcript levels at blood collectionConvenient collection and storage of small blood volumesRapid purification of high-quality total RNAEffective recovery of miRNAThe RNeasy Protect Animal Blood System provides a complete solution for preparing high-quality RNA from animal blood. Blood from mouse, rat, and other small animals is conveniently collected in RNAprotect Animal Blood Tubes, which contain a reagent that immediately stabilizes cellular RNA. Total RNA is then purified using the RNeasy Protect Animal Blood Kit. Please note that the RNeasy Protect Animal Blood Kit and the RNAprotect Animal Blood Tubes are ordered separately.Alternatively, the RNeasy Protect Animal Blood Kit can be used to obtain microRNA (miRNA), either in a total RNA fraction or in a separate small RNA fraction. For purification of total RNA containing miRNA, Buffer RWT (cat. no. 1067933) is also required. For purification of small RNA (containing miRNA) and large RNA in 2 separate fractions, the RNeasy MinElute Cleanup Kit (cat. no. 74204) is also required.The procedures can be fully automated on theQIAcube Connect.Buy ProductsProduct DetailsSpecificationsProduct ResourcesBuy ProductsCat No./ID:73224RNeasy Protect Animal Blood Kit (50)$520.00Order ProductFor 50 preps: RNeasy MinElute Spin Columns, QIAshredder Spin Columns, Collections Tubes, ProteinaseK, RNase-Free DNase, and RNase-Free Reagents and Buffers; to be used with RNAprotect Animal Blood TubesCat No./ID:76544RNAprotect Animal Blood Tubes (50x100µl)$176.00Order Product50 tubes for collection, storage, and transport of 100µl animal blood samples with RNA stabilization; to be used with the RNeasy Protect Animal Blood KitCat No./ID:76554RNAprotect Animal Blood Tubes (50x500µl)$316.00Order Product50 tubes for collection, storage, and transport of 500µl animal blood samples with RNA stabilization; to be used with the RNeasy Protect Animal Blood KitThe RNeasy Protect Animal Blood System is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.Product Details Reproducible RNA yields.Blood was collected from 4 rats in RNAprotect Animal Blood Tubes (four 500 μl samples collected per rat), and RNA was purified using the RNeasy Protect Animal Blood Kit. RNA was quantified using a NanoDrop spectrophotometer. Variability in RNA yield (%CV) between blood samples from the same rat was between 2.4 and 5.9%; between rats, variability was 15%.RNeasy Protect Animal Blood flowchart.The RNeasy Protect Animal Blood System provides a complete solution for preparing high-quality RNA from animal blood. Blood from mouse, rat, and other small animals is conveniently collected in RNAprotect Animal Blood Tubes, which contain a reagent that immediately stabilizes cellular RNA. Total RNA is then purified using the RNeasy Protect Animal Blood Kit. Alternatively, the RNeasy Protect Animal Blood Kit can be used in combination with Buffer RWT to obtain total RNA, including miRNA.Highly intact RNA.Mouse blood (100 μl) was collected in RNAprotect Animal Blood Tubes and stored at 4°C for [A] 2 hours or [B] 2 weeks. RNA was purified using the RNeasy Protect Animal Blood Kit and analyzed on the Agilent 2100 Bioanalyzer. The RIN values were virtually the same, demonstrating preservation of RNA integrity.Effective transcript stabilization.Blood samples (500 μl) from the same rat were collected in RNAprotect Animal Blood Tubes or EDTA-containing tubes. Samples were stored at 15–25°C for 0 minutes (t=0) to 6 hours. RNA was purified using the RNeasy Protect Animal Blood Kit, and FOS expression was analyzed in duplicate by real-time RT-PCR. ΔCT on the Y-axis shows CT values subtracted from those for the t=0 samples. FOS expression remained stable in the RNAprotect stabilized samples, but changed with time in the EDTA-treated samples (equivalent results were observed with other rats; data not shown).PerformanceBlood samples collected in RNAprotect Animal Blood Tubes can be conveniently shipped at ambient temperature or easily stored in a refrigerator. Samples can be stored at 15–25°C for up to 48 hours, at 2–8°C for up to 2 weeks, or at –20°C for at least 3 months with no significant RNA degradation (see figure "Highly intact RNA"). Collection of animal blood in RNAprotect Animal Blood Tubes prevents changes in transcript levels, ensuring reliable gene expression analysis in applications such as real-time RT-PCR and microarray analysis (see figure "Effective transcript stabilization"). The RNeasy Protect Animal Blood Kit delivers reproducible yields of RNA, and requires less than 30 minutes of hands-on time (see figure "Reproducible RNA yields"). Proven RNeasy technology delivers purified RNA that is highly pure, with virtually no genomic DNA contamination.PrincipleWhen blood samples are collected, the gene expression profile can change significantly within minutes. Anticoagulants such as EDTA prevent blood clotting, but do not prevent changes in gene expression due to induced transcription or regulated turnover of mRNA. Collection of animal blood in RNAprotect Animal Blood Tubes prevents changes in transcript levels. Following digestion and homogenization of the stabilized blood, RNA is then purified from the samples using well-established RNeasy silica-membrane technology (see figure "RNeasy Protect Animal Blood flowchart").Procedure Blood samples are collected in RNAprotect Animal Blood Tubes. The tubes contain a reagent that lyses blood cells and stabilizes intracellular RNA. The RNAprotect Animal Blood Tubes are centrifuged to pellet the samples, which are then washed with water and resuspended in Buffer RSB. After digestion in Buffer RBT with proteinase K, the samples are homogenized by centrifugation through QIAshredder spin columns. Ethanol is added to the samples, and the samples are then centrifuged through RNeasy MinElute spin columns. The bound total DNA is subjected to DNase digestion and washed with Buffer RW1, followed by Buffer RPE. Pure RNA is eluted in Buffer REB. The purification of RNA with the RNeasy Protect Animal Blood Kit can be fully automated on the QIAcube. A separate protocol is provided for isolation of total RNA including miRNA and requires wash Buffer RWT (ordered separately; cat. no. 1067933). For purification of small RNA (containing miRNA) and large RNA in 2 separate fractions, the RNeasy MinElute Cleanup Kit is also required. Applications Total RNA and miRNA purified with the RNeasy Protect Animal Blood System can be used for many downstream applications, including: Gene expression analysis RT-PCR Quantitative real-time RT-PCR Differential display cDNA synthesisSpecificationsFeaturesSpecifications ApplicationsNorthern, dot, and slot blotting, end-point RT-PCR, quantitative, real-time RT-PCR, array analysis, next-generation sequencing Elution volume14 µl FormatSpin column Main sample typeTissue, cells ProcessingManual (centrifugation) Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinTotal RNA Sample amount100 µl or 500 µl animal blood TechnologySilica technology YieldRat blood (500 μl): 10–30 µg; Mouse blood (100 μl): 4–8 µgProduct ResourcesYou are not authorized to download the resourceBrochures & Guides (1) Sort optionsSort alphabetically All insights start with the sample: Your comprehensive guide for isolating top-quality RNAShow detailsShow allShow lessFAQs (7) Sort optionsSort alphabetically How can I check the integrity of RNA purified using RNeasy Kits?FAQ ID -1024ViewEffects of low A260/A230 ratios in RNA preparations on downstream applicationsFAQ ID -2248ViewWhat is the composition of Buffer RLT?FAQ ID -2793ViewWhat is the composition of Buffer RW1? FAQ ID -2796ViewWhat is the composition of Buffer RPE? FAQ ID -2797ViewDo you have a kit for RNA isolation from any kind of sample type?FAQ ID -627ViewWhat can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?FAQ ID -728ViewShow allShow lessKit Handbooks (1) Sort optionsSort alphabetically RNeasy Protect Animal Blood HandbookShow detailsShow allShow lessQuick-Start Protocols (1) Sort optionsSort alphabetically RNeasy Protect Animal Blood Kit (EN)Show detailsShow allShow lessPackage Insert (1) Sort optionsSort alphabetically RNAprotect Animal Blood Tubes Product SheetFor collection and RNA stabilization of animal bloodShow detailsShow allShow lessSafety Data Sheets (3) Sort optionsSort alphabetically MSDS RNeasy Protect Animal Blood Kit (50)MSDS RNAprotect Animal Blood Tubes (50x100µl)MSDS RNAprotect Animal Blood Tubes (50x500µl)Show allShow lessfragment fix placeholderCustomers who bought these products also boughtCat No./ID:1067933Buffer RWT (80 ml)80 ml Buffer RWT concentrate$135.00Add To CartCat No./ID:330603RT² SYBR Green FAST Mastermix (24)Contains 24 x 1.35 mltubes: for 24 x 100-well ring discs on the QIAGEN Rotor-Gene Q or 3000 x 20 µl reactions$1,684.00Add To CartCat No./ID:334376QIAseq FastSelect –Globin Kit (24)Includes3 tubes of QIAseq FastSelect reagent forglobin mRNAremoval; sufficient for24reactions from human, mouse and rat samples$1,080.00Add To CartCat No./ID:940014BioSprint 15 DNA Blood Kit (45)For 45 preps: 5-Rod Covers, 5-Tube Strips, MagAttract Suspension G, Buffers and Reagents$170.00Add To CartCat No./ID:76104RNAprotect Tissue Reagent (50ml)50 ml RNAprotect Tissue Reagent for stabilization of RNA in 25 x 200 mg tissue samples$88.60Add To CartCat No./ID:1030058BioSprint 15 Plasticware (130)26 x 5-Rod Covers and 130 x 5-Tube Strips for use with the BioSprint 15$151.00Add To CartCat No./ID:338114Mouse XpressRef Universal Total RNA2 tubes each containing 100 μg Mouse XpressRef Universal Total RNA$317.00Add To CartCat No./ID:218075miScript SYBR Green PCR Kit (1000)For 1000 reactions: QuantiTect SYBR Green PCR Master Mix, miScript Universal Primer$2,068.00Add To CartCat No./ID:335376QIAseq FastSelect –rRNA/Globin Kit (24)Includes3 tubes of QIAseq FastSelect reagent for rRNA removal and3 tubes of QIAseq FastSelect reagent for globin mRNA removal; sufficient for24 reactions from human, mouse and rat samples$1,989.00Add To CartCat No./ID:1014630Buffer AP1 (200/250),KGBuffer AP1 (200/250),KG$87.60Add To CartCat No./ID:218761miScript Plant RT Kit (50)For fifty 20 µl first strand cDNA synthesis reactions; 10x miScript Ligation Buffer, 10x miScript Plant Adaptor, 2x miScript Ligation Activator, miScript Plant RNA Ligase, 5x miScript RT Buffer, 10x miScript Plant RT Nucleics, miScript Plant Reverse Transcriptase, RNase-Free Water$520.00Add To CartCat No./ID:150025REPLI-g Mini Kit (100)DNA Polymerase, Buffers, and Reagents for 100 x 50 µl whole genome amplification reactions (typical yield 10 µg per reaction)$705.00Add To CartCat No./ID:929500QX Intensity Calibration Marker (1 ml)1 ml QX Intensity Calibration Marker$81.40Add To CartCat No./ID:76154RNAprotect Tissue Tubes (50 x 1.5 ml)For stabilization of RNA in 50 x 150 mg tissue samples: 50 screw-top tubes containing 1.5 ml RNAprotect TissueReagent each$289.00Add To CartCat No./ID:158388Gentra Puregene Cell Kit (6.7 x 109)For (6.7 x 109) cells: Reagents$1,034.00Add To CartCat No./ID:13116-400Glass Beads, 0.5 mm (400 g)Customize your bead tubes with bulk beads for rapid and reliable biological sample lysis from a wide variety of starting materials.$264.00Add To CartCat No./ID:204854QuantiFast Multiplex RT-PCR Kit (400)For 400 x 25 µl reactions: 3 x 1.7 ml 2x QuantiFast Multiplex RT-PCR Master Mix (with ROX dye), 100 µl QuantiFast RT Mix, 2 x 2 ml RNase-Free Water$1,006.00Add To CartCat No./ID:10043QIAGEN-tip 100 (25)25 columns$300.00Add To CartCat No./ID:929606QX FA Separation Buffer (40 ml)40 ml QX FA Separation Buffer; for use with the QIAxcel DNA Fast Analysis Kit$74.50Add To CartCat No./ID:929530 QX Alignment Marker 15 bp/600 bp (1.5 ml) Alignment marker with 15 bp and 600 bp fragments$97.00Add To Cart