产品说明
Sample Collection & StabilizationDNA5RNA19Tissue/FFPE5RNAprotect Cell ReagentPrint For immediate stabilization and convenient transport of sorted or cultured cellsImmediate RNA stabilization with no need to remove mediumCells stable at 30ºC (1 day), 15–25ºC (7 days), or 2–8ºC (4 weeks)Archiving of cells at –20ºC or –80ºC RNAprotect Cell Reagent provides immediate stabilization of RNA in sorted or cultured cells. Cells are stabilized at room temperature and can be stored or transported at ambient temperature prior to RNA purification. Buy ProductsProduct DetailsProduct ResourcesBuy ProductsCat No./ID:76526RNAprotect Cell Reagent (250 ml)$363.00Order Product250 ml RNAprotect Cell ReagentThe RNAprotect Cell Reagent is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.Product Details More reliable quantification of CD83 transcript.Effective inhibition of PMA induction of c-fos expression.PerformanceRNAprotect Cell Reagent prevents induction of gene expression in treated cells and preserves and stabilizes transcript levels during storage of cell samples (see figures " Effective inhibition of PMA induction of c-fos expression" and " More reliable quantification of CD83 transcript").Principle RNAprotect Cell Reagent provides immediate stabilization of RNA in sorted or cultured cells. Cells are stabilized at room temperature and can be stored or transported at ambient temperature prior to RNA purification. ProcedureRNAprotect Cell Reagent immediately stabilizes the gene expression profile in cells. With other technologies, it is necessary to wash or process the cells (e.g., trypsin treatment) prior to adding an RNA stabilization reagent. Changes in expression that may occur during the washing or processing steps are eliminated by adding RNAprotect Cell Reagent directly to cells in culture medium or buffer. Instantaneous RNA stabilization with RNAprotect Cell Reagent can also be extended to pelleted cells not in medium or storage solution. The amount of starting material must not exceed 1 x 107 cells.Applications Cells in RNAprotect Cell Reagent can be conveniently stored and transported at ambient temperature before further processing and RNA purification. The RNA can be purified using the RNeasy Plus Mini Kit, or purified simultaneously with DNA from the same cell sample using an AllPrep Kit. Product ResourcesYou are not authorized to download the resourceBrochures & Guides (3) Sort optionsSort alphabetically All insights start with the sample: Your comprehensive guide for isolating top-quality RNAShow details(EN) - Stabilization Solutions for Reliable Gene Expression AnalysisShow details(EN) - Analyzing Gene Expression and RegulationShow detailsShow allShow lessFAQs (5) Sort optionsSort alphabetically Can you recommend a Reagent or Kit for stabilizing RNA in cultured cells?FAQ ID -1215ViewCan cells stabilized with RNAprotect Cell Reagent be used for cell sorting by flow cytometry (FACS)?FAQ ID -1216ViewIs RNAprotect Cell Reagent provided in the RNeasy Protect Cell Kit available separately?FAQ ID -1217ViewI accidentally added RNAprotect Tissue Reagent to my cells, and now the cells are difficult to pellet. What can I do?FAQ ID -364ViewWhy is it not recommended to stabilize cells with RNAprotect Tissue Reagent?FAQ ID -941ViewShow allShow lessKit Handbooks (1) Sort optionsSort alphabetically RNAprotect Cell Reagent HandbookRNAprotect Cell ReagentFor immediate stabilization of nucleic acids in cellsRNeasy Protect Cell Mini KitFor immediate stabilization of nucleic acids in cellsand subsequent total RNA purificationShow detailsShow allShow lessSafety Data Sheets (2) Sort optionsSort alphabetically MSDS RNAprotect Cell Reagent (250 ml)CofAShow allShow lessfragment fix placeholderCustomers who bought these products also boughtCat No./ID:74624RNeasy Protect Cell Mini Kit (50)RNAprotect Cell Reagent (50 ml) and RNeasy Plus Mini Kit (50)$391.00Add To CartCat No./ID:76104RNAprotect Tissue Reagent (50ml)50 ml RNAprotect Tissue Reagent for stabilization of RNA in 25 x 200 mg tissue samples$88.60Add To CartCat No./ID:76106RNAprotect Tissue Reagent (250ml)250 ml RNAprotect TissueReagent for stabilization of RNA in 125 x 200 mg tissue samples$369.00Add To CartCat No./ID:79654QIAshredder (50)50 disposable cell-lysate homogenizers for use in nucleic acid minipreps, caps$93.30Add To CartCat No./ID:206047Type-it Fast SNP Probe PCR Kit (4000)For 4000 x 25 μl reactions: 2 x 25 ml of 2x Type-it Fast SNP Probe PCR Master Mix, 5x Q-Solution, RNase-Free Water$3,110.00Add To CartCat No./ID:28306QIAquick Nucleotide Removal Kit (250)250 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)$573.00Add To CartCat No./ID:74134RNeasy Plus Mini Kit (50)For 50 minipreps: RNeasy Mini Spin Columns, gDNA Eliminator Spin Columns, Collection Tubes, RNase-Free Water and Buffers$374.00Add To CartCat No./ID:67563MagAttract HMW DNA Kit (48)For 48 DNA preps: MagAttract Suspension G, Buffer ATL, Buffer AL, Buffer MB, Buffer MW1, Buffer PE, Proteinase K, RNase A, Buffer AE, Nuclease-Free Water$339.00Add To CartCat No./ID:76405Allprotect Tissue Reagent (100 ml)100 ml Allprotect Tissue Reagent, Allprotect Reagent Pump$684.00Add To CartCat No./ID:79656QIAshredder (250)250 disposable cell-lysate homogenizers for use in nucleic acid minipreps, caps$406.00Add To CartCat No./ID:150370REPLI-g Single Cell Cryo-Protect Reagent (15 ml)Single cell reagent protects cell integrity during isolation, storage and shipping$304.00Add To CartCat No./ID:74004RNeasy Micro Kit (50)50 RNeasy MinElute Spin Columns, Collection Tubes (1.5ml and 2ml), RNase-free DNase I, Carrier RNA, RNase-free Reagents and Buffers$522.00Add To CartCat No./ID:12866-25RNeasy PowerSoil Total RNA KitFor the isolation of high quality total RNA from all soil types$362.00Add To CartCat No./ID:79254RNase-Free DNase Set (50)1500 Kunitz units RNase-free DNase I, RNase-free Buffer RDD, and RNase-free water for 50 RNA minipreps$113.00Add To CartCat No./ID:79256RNase-Free DNase Set (250)5 x 1500 Kunitz units RNase-free DNase I, RNase-free Buffer RDD, and RNase-free water for 250 RNA minipreps$512.00Add To Cart
QIAGEN是一家专业化致力于生物分子样品制备解决方案的跨国经营企业,总部位于德国。1984年,QIAGEN在德国成立,1996年在美国纽约纳斯达克上市。
QIAGEN拥有超过1000项专利和认可证明,在18个国家设立了分公司,代理商服务国超过40个,在全球有超过400000的用户。QIAGEN提供的产品超过500类,包括各种试剂,耗材和自动化纯化工作站。这些产品用于样品采集,稳定,核酸或蛋白的分离,纯化和检测中,不仅广泛的应用于科研领域的各个方面,在生物技术,制药,法医研究,食品安全检测,畜牧业和分子诊断领域也得到了广泛的应用。QIAGEN产品的卓越品质和在应用中的出色表现使得其成为样品处理中标准的代名词。
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