Sample Collection & StabilizationDNA5RNA19Tissue/FFPE5RNAprotect Bacteria ReagentPrint For stabilization of RNA in bacterial culturesImmediate RNA stabilizationConvenient and safe handling at room temperatureStabilization of Gram-positive and Gram-negative bacteriaImmediate RNA protection to ensure reliable work RNAprotect Bacteria Reagent provides immediate stabilization of RNA prior to RNA isolation procedures, thereby ensuring reliable gene expression and gene-profiling data. Adding the reagent directly to bacterial cultures achieves stabilization of both Gram-positive and Gram-negative bacteria. Buy ProductsProduct DetailsProduct ResourcesBuy ProductsCat No./ID:76506RNAprotect Bacteria Reagent$283.00Order ProductRNAprotect Bacteria Reagent (2 x 100 ml)The RNAprotect Bacteria Reagent is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.Product Details RNAprotect Bacteria Reagent prevents mRNA degradation.In order to monitor mRNA degradation only, transcription was stopped by adding the RNA polymerase inhibitor rifampicin to a growing culture of E. coli. The culture was split into halves, and RNAprotect Bacteria Reagent was added to one half. Samples were left at room temperature for 0, 4, 8, and 16 minutes before centrifugation and RNA isolation. The resulting RNA was analyzed by agarose gel electrophoresis (top panel). The expression of two marker genes with different half-lives was examined by northern blot analysis. Middle panel: ompA (half life of 15 minutes); bottom panel: α-lactamase (half life of 2–5 minutes).RNAprotect Bacteria Reagent procedure.GeneChip analysis.Total RNA was isolated from RNAprotect stabilized E. coli cultures using the RNeasy Protect Bacteria Kit (RNeasy Protect Bacteria) or from unstabilized cultures using a commercial precipitation method (Supplier EIII). To distinguish between gene expression under defined culture conditions and effects of artifactual gene induction during harvesting and RNA isolation, the RNA polymerase inhibitor rifampicin was added to half of the culture prior to RNA isolation. Differences in transcript levels with and without rifampicin therefore generally reflect the degree of RNA degradation. [A] GeneChip analysis of E.coli microarrays was carried out according to standard Affymetrix protocols. [B] The percentage of genes expressed was estimated as the number of different transcripts determined present by GeneChip analysis divided by the total number of transcripts represented on the microarray. (Data from a collaborative study with Affymetrix.)Performance Use of RNAprotect Bacteria Reagent overcomes the problems of RNA degradation and changes in expression profiles by providing immediate stabilization prior to RNA isolation (see figures "RNAprotect Bacteria Reagent prevents mRNA degradation" and "GeneChip analysis"). RNAprotect Bacteria Reagent is suitable for use with a wide range of bacterial species, both Gram positive (e.g., Staphylococcus aureus and Mycobacterium avium) and Gram negative (e.g., Escherichia coli and Salmonella typhimurium). Bacteria can be grown in either minimal or complex medium prior to isolation of RNA. Principle Traditional methods for cell harvesting and RNA isolation can lead to vast changes in bacterial expression profiles. Enzymatic degradation of RNA leads to reduction or loss of many transcripts because bacterial mRNA molecules have very short half lives of only a few minutes. In addition, genes can be induced during handling and processing of samples, leading to higher expression levels of certain transcripts. Use of RNAprotect Bacteria Reagent overcomes these problems by providing immediate stabilization of RNA prior to RNA isolation procedures. Procedure Two volumes of reagent are added directly to 1 volume of bacterial culture prior to RNA isolation, providing immediate stabilization of RNA (see figure "RNAprotect Bacteria Reagent procedure"). The stabilization allows time for efficient bacterial lysis using a choice of protocols: enzymatic lysis, mechanical disruption, or a combination of both methods. QIAGEN recommends the TissueLyser for efficient mechanical disruption. Applications Bacteria stabilized in RNAprotect Bacteria Reagent can be used for total RNA isolation using RNeasy Kits. Product ResourcesYou are not authorized to download the resourceBrochures & Guides (3) Sort optionsSort alphabetically All insights start with the sample: Your comprehensive guide for isolating top-quality RNAShow details(EN) - Stabilization Solutions for Reliable Gene Expression AnalysisShow details(EN) - Analyzing Gene Expression and RegulationShow detailsShow allShow lessFAQs (6) Sort optionsSort alphabetically Do you have a protocol for the isolation of RNA from bacterial cultures using RNAprotect Bacteria Reagent and QIAzol Lysis Reagent?FAQ ID -1020ViewHow much RNA does a bacterial cell contain? FAQ ID -2949ViewHow long can I store bacterial culture stabilized in RNAprotect Bacteria Reagent?FAQ ID -614ViewCan I stabilize RNA in bacteria grown on solid media or substrate using the RNAprotect Bacteria Reagent?FAQ ID -615ViewDo you have a kit for RNA isolation from any kind of sample type?FAQ ID -627ViewIs RNAprotect Bacteria Reagent compatible with the RNeasy Mini Kit?FAQ ID -797ViewShow allShow lessQuick-Start Protocols (1) Sort optionsSort alphabetically RNAprotect Bacteria Reagent and RNeasy Protect Bacteria Kits (EN)Show detailsShow allShow lessKit Handbooks (1) Sort optionsSort alphabetically RNAprotect Bacteria Reagent HandbookShow detailsShow allShow lessSafety Data Sheets (2) Sort optionsSort alphabetically MSDS RNAprotect Bacteria ReagentCofAShow allShow less References 0 fragment fix placeholderCustomers who bought these products also boughtCat No./ID:79254RNase-Free DNase Set (50)1500 Kunitz units RNase-free DNase I, RNase-free Buffer RDD, and RNase-free water for 50 RNA minipreps$113.00Add To CartCat No./ID:74524RNeasy Protect Bacteria Mini Kit (50)RNeasy Mini Kit (50) and RNAprotect Bacteria Reagent (2 x 100 ml)$528.00Add To CartCat No./ID:19131QIAGEN Proteinase K (2 ml)2 ml (>600 mAU/ml, solution)$102.00Add To CartCat No./ID:74104RNeasy Mini Kit (50)50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers$330.00Add To CartCat No./ID:12462 QIAGEN Large-Construct Kit (10) 10 QIAGEN-tip 500, Reagents, Buffers, ATP-Dependent Exonuclease$454.00Add To CartCat No./ID:951336MagAttract DNA Blood Mini M48 Kit (192)MagAttract Suspension B, Buffers, and RNase-Free Water for up to 192 x 200 µl preps$614.00Add To CartCat No./ID:76106RNAprotect Tissue Reagent (250ml)250 ml RNAprotect TissueReagent for stabilization of RNA in 125 x 200 mg tissue samples$369.00Add To CartCat No./ID:204074Rotor-Gene SYBR Green PCR Kit (400)For 400 x 25 µl reactions: 3 x 1.7 ml 2x Rotor-Gene SYBR Green PCR Master Mix, 2 x 2 ml RNase-Free Water$471.00Add To CartCat No./ID:382428Investigator 24plex GO! 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