Sample Collection & StabilizationDNA5RNA19Tissue/FFPE5PAXgene Bone Marrow RNA TubesPrint For collection of bone marrow samples and stabilization/purification of intracellular RNA Integrated system for storage, stabilization, and purificationImmediate stabilization of intracellular RNA at collectionRapid purification from heterogeneous bone marrow samplesSafe and easy transportationThe PAXgene Bone Marrow RNA System consists of PAXgene Bone Marrow RNA Tubes, for stabilization, storage, and transportation of bone marrow, and the PAXgene Bone Marrow RNA Kit, for silica-membrane-based RNA isolation and purification in a spin-column format. Bone marrow samples are collected using standard medical practices, with commonly used anticoagulants. A 2 ml sample is immediately transferred into a PAXgene Bone Marrow RNA Tube, which stabilizes the RNA in the sample for storage at room temperature, refrigerated, or frozen. RNA can be purified days later using the PAXgene Bone Marrow RNA Kit or archived for several months at –20°C or –70°C. Buy ProductsProduct DetailsSpecificationsProduct ResourcesBuy ProductsCat No./ID:764114PAXgene Bone Marrow RNA Tubes (50)$1,139.00Order Product50 Bone Marrow Collection Tubes. To be used in conjunction with the PAXgene Bone Marrow RNA KitFor Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.Product Details Storage under different conditions.Bone marrow samples were stored in PAXgene Bone Marrow RNA Tubes [A] for 3 days at 18–25°C, [B] for 5 days at 2–8°C, or [C] subjected to 2 freeze–thaw cycles. RNA was purified using the PAXgene Bone Marrow RNA Kit, and RNA purity was analyzed by absorbance. The A260/A280 ratios indicate highly pure RNA from samples stored under different conditions.RNA stability at room temperature.Bone marrow samples were collected from 39 individuals and immediately transferred to [A] PAXgene Bone Marrow RNA Tubes or [B] EDTA tubes. RNA was purified using the PAXgene Bone Marrow RNA Kit or a reference silica-membrane-based method at day 0 or after 2 days storage at room temperature. Expression levels of 5 marker transcripts for each sample (IL-8, p53, cox-2, IL-1β, c-fos) were quantified using real-time RT-PCR. The high correlation of CT values (threshold cycles) for the samples stabilized in PAXgene Bone Marrow RNA Tubes indicates that the levels of these transcripts did not significantly change during storage, in contrast to the samples stored in EDTA tubes.RNA yields from bone marrow samples.Bone marrow samples were stabilized in PAXgene Bone Marrow RNA Tubes, and RNA was purified using the PAXgene Bone Marrow RNA Kit. Yields are indicated for 295 samples, from both in-house and external studies. The median yield for the 295 samples was 11.0 μg RNA per 2 ml sample. Note that RNA yields can vary greatly due to the extreme heterogeneity of bone marrow samples.High RNA integrity after freeze–thaw cycles.Bone marrow samples were stabilized in PAXgene Bone Marrow RNA Tubes, and RNA was purified using the PAXgene Bone Marrow RNA Kit after the indicated number of freeze–thaw cycles. The RIN values did not change significantly, indicating that RNA remained intact in the samples up to 2 freeze–thaw cycles.RNA stabilized at 2–8°C.Bone marrow samples were collected in duplicate and stabilized in PAXgene Bone Marrow RNA Tubes. RNA was purified from the samples using the PAXgene Bone Marrow RNA Kit with no storage or after 5 days storage at 2–8°C. RNA integrity was evaluated using the RNA integrity numbers (RIN), measured on the Agilent 2100 Bioanalyzer. Results are shown for 3 representative donors. The RIN did not change significantly during storage, indicating that the RNA remains stable in the sample.Stability of 5 transcripts at room temperature.The changes in mRNA levels (ΔCT) from day 0 to day 2 for the samples in figure "RNA stability at room temperature" were calculated for each transcript quantified. The minimal changes and small standard deviation for samples stabilized in PAXgene Bone Marrow RNA Tubes contrasts with the significant changes for samples stored in EDTA tubes.PerformanceThe PAXgene Bone Marrow RNA Kit enables efficient isolation and purification of intracellular RNA from heterogeneous human whole bone marrow samples (see figure "RNA yields from bone marrow samples"), including an optimized procedure for cell-rich, viscous samples. RNA can be purified days after collection, and intact, high-quality RNA can be purified using the PAXgene Bone Marrow RNA Kit after samples are stored at room temperature, refrigerated, or frozen (see figures "RNA stability at room temperature", "RNA stabilized at 2–8°C", and "High RNA integrity after freeze–thaw cycles"). RNA Tubes protect RNA molecules from degradation by RNases and enable accurate analysis byminimizing ex vivo changes in gene expression (see figure "Stability of 5 transcripts at room temperature").An integrated DNase treatment ensures that genomic DNA is minimized. In-house and external studies showed that genomic DNA was <0.85% in 95% of the samples tested (n = 157). A260/A280 values are typically between 1.8 and 2.2, indicating the high purity of the RNA (see figure "Storage under different conditions").Principle Copy numbers of individual mRNA species in bone marrow can change significantly during storage or transport at room temperature, making reliable studies of gene expression impossible. PAXgene Bone Marrow RNA Tubes protect RNA molecules from degradation and immediately stabilize the RNA in the sample for storage at room temperature, refrigerated, or frozen. Procedure Bone marrow samples are collected using standard medical practices, with commonly used anticoagulants. A 2 ml sample is then transferred into a PAXgene Bone Marrow RNA Tube. The RNA in the sample is immediately stabilized for storage at room temperature, refrigerated, or frozen. The PAXgene Bone Marrow RNA Kit provides an easy and rapid procedure for isolation and purification of intracellular RNA from human whole bone marrow samples. The kit uses silica-membrane technology in a spin-column format for efficient purification of intact, high-quality RNA. Applications RNA purified using the PAXgene Bone Marrow RNA Kit is ready for use in a wide range of downstream applications, including Real-time RT-PCR analysis of specific transcripts Array analysis of complex expression profiles SpecificationsFeaturesSpecifications Additive/concentrationAdditive 11.1 ml Closure type/colorBD Hemogard/Red Draw volume2 ml Label typePaper Quantity50/box Tube size16 x 125 mm Product ResourcesYou are not authorized to download the resourceBrochures & Guides (3) Sort optionsSort alphabetically All insights start with the sample: Your comprehensive guide for isolating top-quality RNAShow details(EN) - Analyzing Gene Expression and RegulationShow details(EN) - PAXgene Bone Marrow RNA System BrochureFor collection, storage, and transport of bone marrow samples and stabilization and purification of intracellular RNAShow detailsShow allShow lessFAQs (17) Sort optionsSort alphabetically Can the PAXgene Bone Marrow RNA System be used with animal bone marrow or with blood samples?FAQ ID -2501ViewCan the PAXgene Bone Marrow RNA System be used for other human body fluids than bone marrow samples or cultured cells of human origin?FAQ ID -2502ViewCan bone marrow aliquots other than 2 mL be pipetted into the PAXgene Bone Marrow RNA Tube?FAQ ID -2503ViewHow much time may the aspirated bone marrow sample be allowed to incubate before pipetting it into the PAXgene Bone Marrow RNA Tube?FAQ ID -2504ViewHow should the PAXgene Bone Marrow RNA Tubes be frozen after collection and for how long?FAQ ID -2505ViewWhat RNA yields are expected with the PAXgene Bone Marrow RNA System?FAQ ID -2506ViewCan the PAXgene Bone Marrow RNA System be used to isolate genomic DNA or viral RNA and DNA?FAQ ID -2507ViewCan specific types of white blood cells be enriched from a PAXgene Bone Marrow RNA Tube prior to the RNA isolation?FAQ ID -2508ViewIs RNA from red blood cells also isolated when RNA is extracted from a PAXgene Bone Marrow RNA Tube?FAQ ID -2509ViewCan bone marrow from leukemia patients with elevated numbers of white blood cells be processed with the PAXgene Bone Marrow RNA System?FAQ ID -2510ViewWhat is in the elution buffer in the PAXgene Bone Marrow RNA kit?FAQ ID -2511ViewCan I use water to elute the RNA in the PAXgene Bone Marrow RNA Kit?FAQ ID -2512ViewI routinely heat an aliquot of the RNA eluate prior to my RT reaction. Do I still need to heat the eluate after the PAXgene Bone Marrow RNA preparation?FAQ ID -2513ViewIs the 260/230 nm absorbance ratio specified for RNA derived from the PAXgene Bone Marrow RNA System?FAQ ID -2514ViewCan the PAXgene RNA stabilization solution/bone marrow mixture from the PAXgene Bone Marrow RNA Tube be disposed of by adding bleach?FAQ ID -2515ViewHow are the supernatants resulting from the first two spin steps of the PAXgene Bone Marrow RNA kit purification procedure disposed? FAQ ID -2516ViewWhere can I find additional information for PreAnalytiX PAXgene products? FAQ ID - 3515ViewShow allShow lessKit Handbooks (2) Sort optionsSort alphabetically (EN) - PAXgene Bone Marrow RNA Kit HandbookFor isolation and purification of intracellular RNA from whole bonemarrow samples stabilized in PAXgene Bone Marrow RNA TubesShow details(EN) - PAXgene Bone Marrow Tube product circularFor the collection of human bone marrow and immediate stabilization of intracellular RNAShow detailsShow allShow lessScientific Posters (2) Sort optionsSort alphabetically (EN) - Evaluation of the PAXgene Bone Marrow RNA System for Stabilization and Purification of Cellular RNAGuenther et al., AMP 2006Show details(EN) - RNA Stabilization of Human Whole Bone Marrow Aspirates for High Reliability of Gene Expression AnalysisGuenther et al., AACR 2007Show detailsShow allShow lessSafety Data Sheets (1) Sort optionsSort alphabetically MSDS PAXgene Bone Marrow RNA Tubes (50)Show allShow lessfragment fix placeholderCustomers who bought these products also boughtCat No./ID:764133PAXgene Bone Marrow RNA Kit (30)For 30 RNA preps: 30 PAXgene Spin Columns, 30PAXgene Shredder Spin Columns, Processing Tubes, RNase-free DNase I, RNase-free reagents and buffers. 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