Sample Collection & StabilizationDNA5RNA19Tissue/FFPE5PAXgene 96 Blood RNA KitPrint For high-throughput purification of cellular RNA from whole bloodParallel purification of RNA from 96 samplesStandardized sample processing prior to analysis No organic extraction or precipitation Isolation of high-quality cellular RNA The PAXgene 96 Blood RNA Kit is for high-throughput purification of cellular RNA in a manual format. Used with PAXgene Blood RNA Tubes (available from BD, cat. no. 762165) for sample collection and stabilization, the system provides a complete preanalytical solution for collection and stabilization through to purification of high-quality RNA to support high-throughput projects, such as clinical research studies. Buy ProductsProduct DetailsSpecificationsProduct ResourcesBuy ProductsCat No./ID:762331PAXgene 96 Blood RNA Kit (4)$3,870.00Order Product4 PAXgene 96 RNA Plates, 4 PAXgene 96 Filter Plates, Buffers, Proteinase K, RNase-free DNase Sets, AirPore Tape Sheets, Collection Vessels. To be used in conjunction with PAXgene Blood RNA Tubes.Cat No./ID:9238279PAXgene 96 Incubator Block$339.00Order ProductBlock for denaturation of eluates in PAXgene 96 proceduresFor Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.Product Details Stabilization of gene expression.Blood was collected and RNA was isolated using the PAXgene Blood RNA System (for RNA stabilization and isolation). The graph shows changes in expression of 12 genes after blood collection, measured using real-time RT-PCR. (Data kindly provided by Source Precision Medicine, Boulder, Colorado, USA.)Detection of tyrosinase following blood storage.Blood samples were collected from 6 different donors, spiked with tumor cells expressing tyrosinase, and stored for up to 10 days at room temperature. RT-PCR was used to measure levels of tyrosinase.  [A]  1 cell per 2.5 ml blood;  [B]  10 cells per 2.5 ml blood. WB: tyrosinase detection in RNA isolated from whole blood collected and stored in standard EDTA tubes. PAX: tyrosinase detection using the PAXgene Blood RNA System for sample collection, and RNA stabilization and isolation. (Data kindly provided by LMO, Munich, Germany.)PAXgene 96 Blood RNA procedure.RNA isolation begins with a centrifugation step to pellet nucleic acids in the PAXgene Blood RNA Tube. The pellet is washed, and proteinase K is added to digest proteins. Alcohol is added to adjust binding conditions. Lysates are applied to the PAXgene 96 Filter Plate and centrifuged to remove cell debris. The lysate is then applied to a PAXgene 96 RNA Plate. Residual DNA is removed through a DNase I digestion. Remaining contaminants are removed in three efficient wash steps before pure RNA is eluted.RNA isolation from blood using standard methods.Blood was collected and RNA was isolated using standard methods (collection in EDTA tubes; no stabilization; RNA isolation using a guanidinium-based method). The graph shows changes in expression of 12 genes after blood collection, measured using real-time RT-PCR. (Data kindly provided by Source Precision Medicine, Boulder, Colorado, USA.)PerformanceThe PAXgene 96 Blood RNA Kit allows high-throughput isolation of total RNA from human whole blood. Together with PAXgene Blood RNA Tubes (available from BD, cat. no. 762165), it provides a complete preanalytical solution for collection and stabilization through to purification of high-quality RNA. The robust PAXgene 96 Blood RNAprocedure is highly reproducible and typically yields between 4 and 20 µg of RNA from 2.5 ml human whole blood with minimal risk of sample-to-sample cross contamination.PAXgene Blood RNA Tubes contain a proprietary blend of reagents that bring about immediate stabilization of RNA. This blend prevents the drastic changes in the cellular RNA expression profiles that normally take place in vitro, after blood collection (see figures "RNA isolation from blood using standard methods", "Stabilization of gene expression", and "Detection of tyrosinase following blood storage"). The high-throughput procedure is simple, fast, and efficient, and processing 96 samples in parallel provides significant time savings in your clinical research studies.Principle In the PAXgene 96 Blood RNA System, blood is collected directly into PAXgene Blood RNA Tubes, and RNA is isolated using the PAXgene 96 Blood RNA Kit. PAXgene Blood RNA Tubes contain a proprietary blend of reagents that bring about immediate stabilization of RNA. Blood samples can be stored in the PAXgene Blood RNA Tubes for up to 5 days at room temperature. RNA is then isolated using silica membrane technology supplied in the PAXgene 96 Blood RNA Kit. The resulting RNA accurately represents the expression profile in vivo and is suitable for use in a range of downstream applications. ProcedureBlood samples (2.5 ml) are collected in PAXgene Blood RNA Tubes (available from BD, cat. no. 762165), and may be stored or transported at room temperature if desired. In the PAXgene 96 Blood RNA procedure, RNA isolation begins with a centrifugation step to pellet nucleic acids in the PAXgene Blood RNA Tube. The pellet is washed, and proteinase K is added to digest proteins. Alcohol is added to adjust binding conditions. Lysates are applied to the PAXgene 96 Filter Plate and centrifuged to remove cell debris. The lysate is then applied to a PAXgene 96 RNA Plate (see flowchart "PAXgene 96 Blood RNA procedure"). Residual DNA is removed through a DNase I digestion. Remaining contaminants are removed in three efficient wash steps before pure RNA is eluted.Applications The RNA isolated from human whole blood is ready for use in a wide range of downstream applications, including: Nucleic acid amplification technologies, such as RT-PCR and NASBA Expression-array and expression-chip analysis Quantitative RT-PCR, including TaqMan technology cDNA synthesis RNase and S1 nuclease protection Northern, dot, and slot blot analysis Primer extension SpecificationsFeaturesSpecifications ApplicationsPCR, real-time PCR, qPCR, NASBA, cDNA synthesis CE/FDA/IVD compatibleResearch use only Elution volume45–60 µl Format96-well plate Main sample typeWhole blood ProcessingManual (centrifugation or vacuum) Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinCellular RNA Sample amount2.5 ml StabilizationYes TechnologySilica technology Time per run or per prep Yield4–20 µgProduct ResourcesYou are not authorized to download the resourceBrochures & Guides (4) Sort optionsSort alphabetically All insights start with the sample: Your comprehensive guide for isolating top-quality RNAShow details(EN) - Analyzing Gene Expression and RegulationShow detailsPAXgene 96 Blood RNA Kit Brochure (EN)For high-throughput purification of cellular RNA from whole bloodShow detailsPAXgene Blood RNA System Brochure (EN)For collection, transport, and storageof whole blood and stabilization and purification of intracellular RNAShow detailsShow allShow lessFAQs (7) Sort optionsSort alphabetically How many PAXgene Blood RNA Tubes can be processed per PAXgene 96 Blood RNA Kit well plate?FAQ ID -2485ViewWhat is the PAXgene 96 filter plate?FAQ ID -2486ViewWhat kits does QIAGEN offer to extract RNA from whole blood?FAQ ID -304ViewCan the PAXgene 96 Blood RNA Kit be used for diagnostic or prognostic procedures? FAQ ID - 3471ViewWhat additional equipment is needed to purify RNA using the PAXgene 96 Blood RNA Kit?FAQ ID - 3474ViewWhere can I find additional information for PreAnalytiX PAXgene products? FAQ ID - 3515ViewI accidentally stored Buffer RDD of the RNase-Free DNase Set at°C. Will it still function? -20ViewShow allShow lessKit Handbooks (1) Sort optionsSort alphabetically PAXgene 96 Blood RNA Kit Handbook - (EN)For isolation of cellular RNA from whole blood Important: To be used only in conjunction with PAXgene Blood RNA tubesShow detailsShow allShow lessSafety Data Sheets (2) Sort optionsSort alphabetically MSDS PAXgene 96 Blood RNA Kit (4)CofAShow allShow lessfragment fix placeholderCustomers who bought these products also boughtCat No./ID:764133PAXgene Bone Marrow RNA Kit (30)For 30 RNA preps: 30 PAXgene Spin Columns, 30PAXgene Shredder Spin Columns, Processing Tubes, RNase-free DNase I, RNase-free reagents and buffers. 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