Sample Collection & StabilizationDNA5RNA19Tissue/FFPE5RNeasy Protect Mini KitPrint For stabilization and purification of up to 100 µg total RNA from tissues Immediate RNA stabilization and protection Reliable gene expression and gene-profiling data No need for liquid nitrogen, dry ice, or phenol Ready-to-use, high-quality total RNA in minutes No CsCl gradients, no LiCl or ethanol precipitation The RNeasy Protect Mini Kit includes RNAprotect TissueReagent for stabilizing RNA in tissue samples, and RNeasy spin columns for purifying up to 100 µg of high-quality RNA using silica-membrane technology.RNA purification can be automated on the QIAcube. Efficient disruption of tissue samples can be achieved using a TissueRuptor or TissueLyser system.Buy ProductsProduct DetailsSpecificationsProduct ResourcesBuy ProductsCat No./ID:74124RNeasy Protect Mini Kit (50)$389.00Order ProductRNAprotect TissueReagent (50 ml), 50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and BuffersCat No./ID:74126RNeasy Protect Mini Kit (250)$1,681.00Order ProductRNAprotect TissueReagent (250 ml), 250RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and BuffersThe RNeasy Protect Mini Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.Product Details RNeasy Mini spin column.For purification of total RNA from formalin-fixed, paraffin-embedded tissue sectionsChanges in mRNA levels.Once a biological sample is harvested, its RNA becomes extremely unstable. Gene induction or down-regulation triggered by sample manipulation must be prevented to enable accurate gene expression analysis.Prevention of degradation of mRNA in tissues.RNA was isolated from fresh rat kidney samples after 0, 5, 10, 15, 30, and 60 minutes, using either standard procedures (Unstabilized) or RNeasy Protect Kits (Stabilized). The RNA isolated was analyzed by agarose gel electrophoresis and expression of GAPDH was examined using northern blot analysis. M: markers.Stable RNA in tissues.PerformanceThe RNA-stabilizing properties of RNAprotect TissueReagent prevent gene induction or down-regulation triggered by sample manipulation, and allow purification of high-quality RNA (see figure "Prevention ofdegradation of mRNA in tissues"). In addition, RNeasy purified RNA from RNAprotect stabilized samples remains intact following storage of tissue samples under a wide variety of conditions (see figure "Stable RNA in tissues").A range of tissue types can be processed with the RNeasy Protect Mini Kit (see table).Total RNA yields obtained with RNeasy Protect Mini KitMouse tissueAmount (mg)Average yield* (µg)Brain1010Liver1043Lung1012Spleen1045* Amounts of RNA can vary due to developmental stage, species, and growth conditions of samples.RNA purified with RNeasy technology has A260/A280 ratios of 1.9–2.1 (measured in 10 mM Tris·Cl, pH 7.5). Since the RNeasy procedure enriches for RNA species >200 nt, RNA yield does not include 5S rRNA, tRNAs, or other low-molecular-weight RNAs.PrincipleThe RNeasy Protect system provides a complete RNA protection and isolation solution, from sample harvesting to pure RNA, in one kit. Proven RNeasy silica-membrane technology in spin-column format, combined with the RNA-stabilizing properties of RNAprotect Tissue Reagent, allows purification of high-quality, intact RNA. Once a biological sample is harvested, its RNA becomes extremely unstable (see figure "Changes in mRNA levels"). The innovative, aqueous RNAprotect Tissue Reagent quickly permeates tissues, stabilizing and protecting the RNA expression pattern. Samples can be archived without risk of RNA degradation, even after multiple freeze–thaw cycles. Following stabilization, RNeasy technology simplifies total RNA isolation by combining the stringency of guanidine-isothiocyanate lysis with the speed and purity of silica-membrane purification (see figure "RNeasy Mini spin column").ProcedureSamples can be stabilized and stored in RNAprotect TissueReagent, included in RNeasy Protect Kits. For RNA purification, samples (0.5–30 mg tissue) are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the RNeasy silica membrane (binding capacity up to 100 µg RNA). RNA binds, and all contaminants are efficiently washed away. Pure, concentrated RNA is eluted in 30–100 µl water (see flowchart "RNeasy Protect Mini procedure"). RNA purification can be automated on the QIAcube.ApplicationsRNA purified with RNeasy technology is ideal for use in all applications. Downstream applications include: Northern, dot, and slot blotting End-point RT-PCR Quantitative, real-time RT-PCR Array analysis Poly A+ RNA selection SpecificationsFeaturesSpecifications ApplicationsPCR, qPCR, real-time RT-PCR, microarray Elution volume30–100 µl FormatSpin column Main sample typeTissue samples ProcessingManual (centrifugation) Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinRNA Sample amount0.5–30 mg StabilizationYes TechnologySilica technology Yield10 –45 µgProduct ResourcesYou are not authorized to download the resourceBrochures & Guides (3) Sort optionsSort alphabetically All insights start with the sample: Your comprehensive guide for isolating top-quality RNAShow details(EN) - Stabilization Solutions for Reliable Gene Expression AnalysisShow details(EN) - Analyzing Gene Expression and RegulationShow detailsShow allShow lessFAQs (35) Sort optionsSort alphabetically How do I safely inactivate biohazardous flow-through material?FAQ ID -12ViewHow should I quantify RNA isolated with RNeasy Kits?FAQ ID -32ViewWhy does my isolated RNA have a low OD 260/280 ratio?FAQ ID -97ViewHow can I avoid little or no RNA yields when using an RNeasy Kit?FAQ ID -28ViewWhy do I have to add beta-mercaptoethanol (beta-ME) to lysis Buffer RLT of the RNeasy Kits?FAQ ID -101ViewHow should RNeasy Kits be stored and how long are they stable?FAQ ID -103ViewHow do you ensure that RNeasy buffers are RNase-free?FAQ ID -113ViewWhat is the difference between disruption and homogenization in the RNeasy System?FAQ ID -139ViewAre RNeasy spin columns sold separately?FAQ ID -159ViewHow can I check for purity of RNA isolated using RNeasy Kits?FAQ ID -1023ViewHow can I check the integrity of RNA purified using RNeasy Kits?FAQ ID -1024ViewWhich kit should I use for RNA isolation from Cartilage?FAQ ID -1026ViewDo I have to discard Buffer RLT with beta-Mercaptoethanol (ß-ME) added to it after 1 month of storage?FAQ ID -1037ViewWhat is the difference between Buffers RLT and RLT Plus?FAQ ID -1043ViewHow can I ensure complete genomic DNA removal when using the RNase-Free DNase Set?FAQ ID -1087ViewCan I clean up my DNase treated RNA samples using RNeasy columns?FAQ ID -286ViewWhat is the maximum binding capacity of RNeasy spin columns?FAQ ID -290ViewEffects of low A260/A230 ratios in RNA preparations on downstream applicationsFAQ ID -2248ViewWhat is the composition of Buffer RLT?FAQ ID -2793ViewWhat is the composition of Buffer RW1? FAQ ID -2796ViewWhat is the composition of Buffer RPE? FAQ ID -2797ViewCan RNAprotect Tissue Reagent be used to store previously isolated RNA?FAQ ID -337ViewI accidentally added RNAprotect Tissue Reagent to my cells, and now the cells are difficult to pellet. What can I do?FAQ ID -364ViewCan I use the RNeasy Mini Kit or RNeasy 96 Kit with fewer than 100 cells?FAQ ID -372ViewHow long can I store tissues preserved in RNAprotect Tissue Reagent?FAQ ID-3750ViewWhat happens if I spin my lysate on the RNeasy Spin Columns at maximum speed?FAQ ID -514ViewWhat has to be done to an RNA sample before loading it onto an Agilent Bioanalyzer?FAQ ID -528ViewIs it possible to isolate both RNA and recombinant 6xHis-tagged protein from the same sample?FAQ ID -532ViewCan the RNase-Free DNase Set be used for DNase digestions of RNA in solution?FAQ ID -619ViewHow can I minimize degradation of RNA from my pancreas sample?FAQ ID -624ViewDo you have a kit for RNA isolation from any kind of sample type?FAQ ID -627ViewWhat can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?FAQ ID -728ViewI accidentally stored Buffer RDD of the RNase-Free DNase Set at°C. Will it still function? -20ViewI ran my RNA out on an agarose gel and can see lots of bands similar to a ladder. Why? FAQ ID -745ViewDo I need to use RNase inhibitors with the RNeasy Kits?FAQ ID -813ViewShow allShow lessKit Handbooks (2) Sort optionsSort alphabetically RNAprotect Handbook - (EN)RNAprotect Tissue Tubes - For collection of harvested animal tissues with immediate stabilization of the gene expression profile, and subsequent transport and storage; RNAprotect Tissue Reagent - For immediate stabilization of the gene expression profile in harvested animal tissuesShow detailsRNeasy Mini Handbook - (EN)RNeasy Mini Kit - For purification of total RNA from animal cells, animal tissues, bacteria, and yeast, and for RNA cleanup; RNeasy Protect Mini Kit - For immediate stabilization of RNA in harvested animal tissues and subsequent total RNA purification; RNeasy Plant Mini Kit - For purification of total RNA from plants and filamentous fungiShow detailsShow allShow lessSupplementary Protocols (2) Sort optionsSort alphabetically Purification of total RNA from tissues using the RNeasy® Plus 96 Kit and vacuum/spin technology - (EN)Show detailsPurification of total RNA from tissues using the RNeasy® Plus 96 Kit and spin technology - (EN)Show detailsShow allShow lessQuick-Start Protocols (1) Sort optionsSort alphabetically RNAprotect Tissue Reagent, RNAprotect Tissue Tubes, and RNeasy Protect Kits (EN)Show detailsShow allShow lessUser-Developed Protocols (1) Sort optionsSort alphabetically Preparation of RNAprotect preserved tissues for histological studies - (EN)Show detailsShow allShow lessSafety Data Sheets (3) Sort optionsSort alphabetically MSDS RNeasy Protect Mini Kit (50)MSDS RNeasy Protect Mini Kit (250)CofAShow allShow less References 0 fragment fix placeholderCustomers who bought these products also boughtCat No./ID:965162QIAamp DNA Blood BioRobot 9604 Kit (12)For 12 x 96 DNA preps: 12 QIAamp 96 Plates, Buffers, QIAGEN Protease, AirPore Tape Sheets, Tape Pad, S-Blocks, Racks with Collection Microtubes (1.2 ml), Caps$4,039.00Add To CartCat No./ID:204245QuantiTect SYBR Green RT-PCR Kit (1000)For 1000 x 50 µl reactions: 25 ml 2x QuantiTect SYBR Green RT-PCR Master Mix, 0.5 ml QuantiTect RT Mix, 20ml RNase-Free Water$3,767.00Add To CartCat No./ID:79256RNase-Free DNase Set (250)5 x 1500 Kunitz units RNase-free DNase I, RNase-free Buffer RDD, and RNase-free water for 250 RNA minipreps$512.00Add To CartCat No./ID:150063REPLI-g WTA Single Cell Kit (24)REPLI-g SensiPhi DNA Polymerase, Buffers, and Reagents for 24 x 60 µl whole transcriptome amplification reactions (typical yield: 20 µg)$1,427.00Add To CartCat No./ID:1117316DTT (1 ml)1M DTT, forensic grade quality; 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