Sample Collection & StabilizationDNA5RNA19Tissue/FFPE5RNeasy Protect Saliva Mini KitPrint For stabilization and purification of total RNA from human salivaImmediate stabilization of RNA in salivaConvenient stabilization at room temperatureStorage and shipping of saliva without ice or dry iceArchiving of saliva samples at –20ºC or –80ºCPurification of high-quality RNA in minutes The RNeasy Protect Saliva Mini Kit includes the RNAprotect Saliva Reagent, which stabilizes RNA in saliva samples to preserve the gene expression profile, and the RNeasy Micro Kit, which purifies and concentrates total RNA using specialized RNeasy MinElute spin columns. Stabilized saliva samples can be conveniently stored or shipped at 37°C (1 day), 15–25°C (14 days), or 2–8°C (4 weeks) before RNA purification. RNA is purified from 200 μl saliva and eluted in a volume of just 14 μl. Buy ProductsProduct DetailsSpecificationsProduct ResourcesBuy ProductsCat No./ID:74324RNeasy Protect Saliva Mini Kit (50)$784.00Order ProductRNAprotect Saliva Reagent (50 ml) and RNeasy Micro Kit (50)The RNeasy Protect Saliva Mini Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.Product Details Minimization of transcript degradation in saliva.Saliva samples were incubated with or without RNAprotect Saliva Reagent at room temperature for 0 or 7 days. After RNA purification, samples were analyzed using Human Genome U133 Plus 2.0 GeneChip Arrays. Comparison between 0 and 7 days shows a 2-fold decrease in signal intensity for 159 transcripts with unstabilized samples, but for only 38 transcripts with RNAprotect stabilized samples (indicated by red spots). (Data kindly provided by Dr. David Wong, University of California, Los Angeles, CA, USA.)Effective stabilization of beta-actin transcript.RNAprotect Saliva Reagent stabilized β-actin mRNA levels in saliva for up to 3 days at 25°C. mRNA levels were quantified by real-time RT-PCR using the QuantiTect Probe RT-PCR Kit. Each time point shows the mean CT value from 4 or 6 samples from a saliva pool from 3 donors.Stable mRNA levels of cancer biomarkers.RNAprotect Saliva Reagent stabilized mRNA levels of oral cancer biomarkers for up to 168 hours. Each time point shows the mean CT value from real-time RT-PCR analysis of 3 samples from a saliva pool from 9 donors. H3F3A: H3 histone, family 3A; OAZ1: ornithine decarboxylase antizyme 1; SAT: spermidine/spermine N1-acetyltransferase; S100P: S100 calcium binding protein P; DUSP1: dual specificity phosphatase 1; IL8: interleukin 8; S100A8: S100 calcium binding protein A8 (an abundant mRNA in saliva; not a cancer biomarker). (Data kindly provided by Dr. David Wong, University of California, Los Angeles, CA, USA.)RNeasy Protect Saliva procedure.PerformanceSaliva stabilized with RNAprotect Saliva Reagent can be stored or transported for up to 1 day at 37ºC, up to 14 days at room temperature (15–25ºC), or up to 4 weeks at 2–8ºC, or can be archived at –20ºC or –80ºC (see figure "Effective stabilization of β-actin transcript"). Purified RNA from saliva, as an alternative to blood, allows microarray analysis for research into oral and systemic diseases, and gene expression analysis of RNA biomarkers for cancer by real-time RT-PCR (see figures "Minimization of transcript degradation in saliva" and "Stable mRNA levels of cancer biomarkers").Principle The RNeasy Protect Saliva Mini Kit provides a complete RNA protection and isolation solution, from sample harvesting to pure RNA, in one kit. Proven RNeasy technology, combined with the RNA-stabilizing properties of RNAprotect Saliva Reagent, allows purification of high-quality, intact RNA. Saliva contains bacteria in addition to cells and free-circulating nucleic acids of human origin. When saliva is collected from a donor and mixed with RNAprotect Saliva Reagent, the RNA is immediately stabilized and uncontrolled growth of bacteria is prevented, ensuring reliable results in downstream gene expression analysis.  Following stabilization, RNeasy technology simplifies total RNA isolation by combining the stringency of guanidine-isothiocyanate lysis with the speed and purity of silica-membrane purification. RNA in bacteria is already degrading prior to collection and stabilization in RNAprotect Saliva Reagent, and will be of lower quality than RNA purified from cultured cells and animal tissues. Procedure Saliva (200 µl) collected from a donor is immediately mixed with RNAprotect Saliva Reagent to stabilize the RNA in the saliva. Total RNA is purified from stabilized saliva using the RNeasy Micro Kit (see flowchart "RNeasy Protect Saliva procedure"). The saliva sample is first centrifuged, and Buffer RLT is then added to the resulting pellet. After addition of ethanol, the sample is applied to an RNeasy MinElute spin column, where RNA binds to the membrane after centrifugation. Traces of genomic DNA are removed by DNase digestion, and contaminants are washed away in several wash steps. Highly pure RNA is then eluted using RNase-free water in a volume of just 14 µl. RNAprotect Saliva Reagent also stabilizes DNA. Protocols for purification of DNA, or RNA and DNA, from stabilized saliva are available.  Applications Total RNA purified from stabilized saliva using the RNeasy Micro Kit represents a noninvasive alternative to blood analysis. The kit allows research into the identification of RNA biomarkers for: Oral diseases Systemic diseases (e.g., cancer) SpecificationsFeaturesSpecifications ApplicationsPCR, qPCR, real-time RT-PCR, microarray Elution volume8–14 µl FormatSpin column Main sample typeSaliva samples ProcessingManual (centrifugation) Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinRNA Sample amount200 µl StabilizationYes TechnologySilica technology YieldVariesProduct ResourcesYou are not authorized to download the resourceBrochures & Guides (3) Sort optionsSort alphabetically All insights start with the sample: Your comprehensive guide for isolating top-quality RNAShow details(EN) - Stabilization Solutions for Reliable Gene Expression AnalysisShow details(EN) - Analyzing Gene Expression and RegulationShow detailsShow allShow lessFAQs (12) Sort optionsSort alphabetically How do you ensure that concentrated RNA is purified from saliva using the RNeasy Protect Saliva Mini Kit?FAQ ID -1212ViewIs a saliva collection device provided in the RNeasy Protect Saliva Mini Kit?FAQ ID -1213ViewCan RNAprotect Saliva Reagent be purchased separately?FAQ ID -1214ViewEffects of low A260/A230 ratios in RNA preparations on downstream applicationsFAQ ID -2248ViewWhat is the composition of Buffer RLT?FAQ ID -2793ViewWhat is the composition of Buffer RW1? FAQ ID -2796ViewWhat is the composition of Buffer RPE? FAQ ID -2797ViewIs it possible to isolate DNA from Saliva Stabilized in RNAprotect® Saliva Reagent?FAQ ID -2995ViewIs it possible to simultaneously isolate DNA and RNA from Saliva Stabilized in RNAprotect® Saliva Reagent?FAQ ID -2996View3326 - Which QIAcube standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet?ViewDo you have a kit for RNA isolation from any kind of sample type?FAQ ID -627ViewWhat can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?FAQ ID -728ViewShow allShow lessKit Handbooks (1) Sort optionsSort alphabetically RNeasy Protect Saliva Mini Handbook - (EN)For immediate stabilization of total RNA in saliva and subsequent RNA purificationShow detailsShow allShow lessSupplementary Protocols (2) Sort optionsSort alphabetically Purification of DNA from Saliva Stabilized in RNAprotect® Saliva Reagent - (EN)Show detailsSimultaneous Purification of DNA and RNA from Saliva Stabilized in RNAprotect® Saliva Reagent - (EN)Show detailsShow allShow lessSafety Data Sheets (1) Sort optionsSort alphabetically MSDS RNeasy Protect Saliva Mini Kit (50)Show allShow lessfragment fix placeholderCustomers who bought these products also boughtCat No./ID:27500-4-EP-BPPowerBead Plates, Glass (4)MagAttract PowerMicrobiome DNA/RNA EP Kit Components$311.00Add To CartCat No./ID:9014579QIAvac MultiwellVacuum manifold for processing 96-well purification plates of SBS standard$888.00Add To CartCat No./ID:27361DirectPrep 96 MiniPrep Kit (4)For 4 x 96 plasmid minipreps: 4 DirectPrep 96 Plates, Buffers, Flat-Bottom Blocks and Lids, AirPore Tape Sheets, Tape Pads, Elution Microtubes RS, Caps$633.00Add To CartCat No./ID:208052QuantiNova SYBR Green PCR Kit (100)For 100 x 20 µl reactions: 1 ml 2x QuantiNova SYBR Green PCR Master Mix, 500 µl QuantiNova Yellow Template Dilution Buffer, 250 µl QN ROX Reference Dye, 1.9 ml Water$116.00Add To CartCat No./ID:208152QuantiNova SYBR Green RT-PCR Kit (100)For 100 x 20 µl reactions: 1 ml QuantiNova SYBR Green RT-PCR Master Mix, 20 µl QuantiNova SYBR Green RT Mix, 20 µl Internal Control RNA, 500 µl Yellow Template Dilution Buffer, 250 µl ROX Reference Dye, 1.9 µl RNase-Free Water$239.00Add To CartCat No./ID:208252QuantiNova Probe PCRKit (100)For 100 x 20 µl reactions: 1 ml 2x QuantiNova Probe PCR Master Mix , 250 µl QN ROX Reference Dye, 500 µl QuantiNova Yellow Template Dilution Buffer, 1.9 ml Water$111.00Add To CartCat No./ID:14900-50-NF-BTPowerWater DNA Bead Tube (50)Bead Tubes for the DNeasy PowerWater Kit$246.00Add To CartCat No./ID:201900dNTP Mix, PCR Grade (200 µl)Mix containing 10 mM each of dATP, dCTP, dGTP, and dTTP (1 x 200 µl)$51.70Add To CartCat No./ID:31314Ni-NTA Spin Kit (50)50 Ni-NTA Spin Columns, Reagents, Buffers, Collection Tubes, 1 μg Control Expression Plasmid$554.00Add To CartCat No./ID:981008PCR Tubes, 0.2 ml  (10000)10 x 1000 thin-walled tubes for 10,000 reactions of 20–50 µl$992.00Add To CartCat No./ID:219610 miRNeasy Serum/Plasma Spike-In Control 10 pmol lyophilized C. elegans miR-39 miRNA mimic$51.30Add To CartCat No./ID:204374Rotor-Gene Probe PCR Kit (400)For 400 x 25 µl reactions: 3 x 1.7 ml 2x Rotor-Gene Probe PCR Master Mix, 2 x 2 ml RNase-Free Water$489.00Add To CartCat No./ID:74524RNeasy Protect Bacteria Mini Kit (50)RNeasy Mini Kit (50) and RNAprotect Bacteria Reagent (2 x 100 ml)$528.00Add To CartCat No./ID:80284 AllPrep DNA/RNA Micro KitFor 50 micropreps: AllPrep DNA Spin Columns, RNeasy MinElute Spin Columns, Collection Tubes, Carrier RNA, RNase-Free Water and Buffers$766.00Add To CartCat No./ID:3691212-Tube MagnetMagnet for separating magnetic beads in 12 x 1.5 ml or 2 ml tubes$399.00Add To CartCat No./ID:763134PAXgene Blood miRNA Kit (50)For 50 RNA preps: PAXgene Spin Columns, PAXgene Shredder Spin Columns, Processing Tubes, Microcentrifuge Tubes, RNase-Free DNase, RNase-Free Reagents and Buffers; to be used in conjunction with PAXgene Blood RNA Tubes$717.00Add To CartCat No./ID:76506RNAprotect Bacteria ReagentRNAprotect Bacteria Reagent (2 x 100 ml)$283.00Add To Cart