Sample Collection & StabilizationDNA5RNA19Tissue/FFPE5RNeasy Protect Cell Mini KitPrint For stabilization and purification of total RNA from cellsImmediate RNA stabilization in sorted or cultured cellsNo need to remove medium prior to cell stabilizationStorage and shipping of cells without dry iceArchiving of cells at –20ºC or –80ºCPurification of high-quality RNA in minutes The RNeasy Protect Cell Mini Kit includes RNAprotect Cell Reagent, which stabilizes RNA and DNA in sorted or cultured cells and allows convenient and reliable transport of the cells at ambient temperature. The stabilized cells can be stored or shipped at 30°C (1 day), 15–25°C (7 days), or 2–8°C (4 weeks). The RNeasy Protect Cell Mini Kit provides the RNeasy Plus Mini Kit for purification of total RNA. Stabilized cells are lysed and spun through gDNA Eliminator spin columns to effectively remove genomic DNA. High-quality RNA is then purified using RNeasy spin columns. The purification procedure can be automated on the QIAcube. Buy ProductsProduct DetailsSpecificationsProduct ResourcesBuy ProductsCat No./ID:74624RNeasy Protect Cell Mini Kit (50)$391.00Order ProductRNAprotect Cell Reagent (50 ml) and RNeasy Plus Mini Kit (50)The RNeasy Protect Cell Mini Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.Product Details More reliable quantification of CD83 transcript.Storage of cytokine-stimulated immature dendritic cells overnight at –20ºC in RNAprotect Cell Reagent instead of PBS allows quantification of higher levels of CD83 mRNA, indicating stabilization of RNA by RNAprotect Cell Reagent. (Data kindly provided by Dr. Alexander Prechtel and Prof. Alexander Steinkasserer, University Hospital Erlangen, Erlangen, Germany.)RNeasy Protect Cell procedure.Effective inhibition of PMA induction.Incubation of PMA-stimulated Jurkat cells in RNAprotect Cell Reagent prevents PMA induction of c-fos expression as well as degradation of c-fos transcript, demonstrating stabilization of RNA by RNAprotect Cell reagent (each bar represents the mean of duplicate cell samples).PerformanceThe RNeasy Protect Cell Mini Kit is ideal for gene expression analysis experiments where cells need to be delivered from one lab to another (see Table 1).Table 1. Higher RNA yields from leukocytes* preserved for 10 days in RNAprotect Cell ReagentAssay resultRNAprotect Cell ReagentRNAprotect TissueReagentRNA yield using RNeasy technology (µg)103.5Real-time RT-PCR of abl (CT value)2526* Leukocytes were isolated on a Ficoll gradient. Data kindly provided by Pascale Cornillet-Lefebvre, Hôpital Robert Debré, Reims, France.Cells in RNAprotect Cell Reagent can be stored or transported for up to 1 day at 30ºC, up to 7 days at room temperature (15–25ºC), or up to 4 weeks at 2–8ºC, and can also be stored long-term at –20ºC or –80ºC (see Table 2, and figures "Effective inhibition of PMA induction" and "More reliable quantification of CD83 transcript").Table 2. Intact RNA from Jurkat cells stored at ambient temperatureCell storage conditionsRIN value of purified RNA*30ºC (1 day)9.825ºC (7 days)9.72–8ºC (28 days)10–20ºC (28 days)9.8–80ºC (28 days)9.8* RNA integrity is assessed on the Agilent 2100 Bioanalyzer, which gives RIN values on a scale of 1 (completely degraded RNA) to 10 (completely intact RNA).Principle The RNeasy Protect Cell Mini Kit provides a complete RNA protection and isolation solution, from sample harvesting to pure RNA, in one kit. Proven RNeasy technology, combined with the RNA-stabilizing properties of RNAprotect Cell Reagent, allows purification of high-quality, intact RNA. Sorted or cultured cells are mixed with RNAprotect Cell Reagent, which immediately stabilizes the cellular RNA, ensuring reliable results in downstream gene expression analysis. RNAprotect Cell Reagent can be added directly to cells in culture medium or buffer, eliminating the need to wash or process cells (e.g., by trypsin treatment). Alternatively, RNAprotect Cell Reagent can be added to pelleted cells. Following stabilization, RNeasy technology simplifies total RNA isolation by combining the stringency of guanidine-isothiocyanate lysis with the speed and purity of silica-membrane purification. ProcedureSorted or cultured cells are mixed with RNAprotect Cell Reagent, which immediately stabilizes the cellular RNA. After RNA stabilization, total RNA is purified using the RNeasy Plus Mini Kit (see flowchart "RNeasy Protect Cell procedure"). The cells in RNAprotect Cell Reagent are centrifuged, and the resulting cell pellet is lysed and homogenized in Buffer RLT Plus. The lysate is then passed through a gDNA Eliminator spin column, which rapidly and effectively removes genomic DNA. Ethanol is added to the lysate, which is then applied to an RNeasy spin column. After centrifugation, total RNA binds to the membrane of the RNeasy spin column. Contaminants are efficiently washed away, and high-quality total RNA is eluted in 30–50 µl of RNase-free water. Purification of DNA, or of RNA and DNA, requires the additional purchase of a QIAGEN DNA purification kit. Details are provided in the RNAprotect Cell Reagent Handbook.ApplicationsCells in RNAprotect Cell Reagent can be transported at ambient temperature, avoiding the need to freeze cell pellets in liquid nitrogen and to ship the frozen pellets on dry ice. RNA purified using the RNeasy Plus Mini Kit is ideally suited for downstream applications that are sensitive to low amounts of DNA contamination, such as: Quantitative real-time RT-PCR SpecificationsFeaturesSpecifications ApplicationsPCR, qPCR, real-time RT-PCR, microarray Elution volume30–50 µl FormatSpin column Main sample typeCultured cells ProcessingManual (centrifugation) Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinTotal RNA Sample amountMax. 10e7 cells StabilizationYes TechnologySilica technology YieldVariesProduct ResourcesYou are not authorized to download the resourceBrochures & Guides (3) Sort optionsSort alphabetically All insights start with the sample: Your comprehensive guide for isolating top-quality RNAShow details(EN) - Stabilization Solutions for Reliable Gene Expression AnalysisShow details(EN) - Analyzing Gene Expression and RegulationShow detailsShow allShow lessFAQs (10) Sort optionsSort alphabetically How can I check the integrity of RNA purified using RNeasy Kits?FAQ ID -1024ViewCan you recommend a Reagent or Kit for stabilizing RNA in cultured cells?FAQ ID -1215ViewIs RNAprotect Cell Reagent provided in the RNeasy Protect Cell Kit available separately?FAQ ID -1217ViewEffects of low A260/A230 ratios in RNA preparations on downstream applicationsFAQ ID -2248ViewWhat is the composition of Buffer RLT?FAQ ID -2793ViewWhat is the composition of Buffer RW1? FAQ ID -2796ViewWhat is the composition of Buffer RPE? FAQ ID -2797ViewI accidentally added RNAprotect Tissue Reagent to my cells, and now the cells are difficult to pellet. What can I do?FAQ ID -364ViewDo you have a kit for RNA isolation from any kind of sample type?FAQ ID -627ViewWhat can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?FAQ ID -728ViewShow allShow lessKit Handbooks (1) Sort optionsSort alphabetically RNAprotect Cell Reagent HandbookRNAprotect Cell ReagentFor immediate stabilization of nucleic acids in cellsRNeasy Protect Cell Mini KitFor immediate stabilization of nucleic acids in cellsand subsequent total RNA purificationShow detailsShow allShow lessSafety Data Sheets (2) Sort optionsSort alphabetically MSDS RNeasy Protect Cell Mini Kit (50)CofAShow allShow lessfragment fix placeholderCustomers who bought these products also boughtCat No./ID:76526RNAprotect Cell Reagent (250 ml)250 ml RNAprotect Cell Reagent$363.00Add To CartCat No./ID:901890636-Well Rotor Locking RingFor locking PCR Tubes, 0.2 ml, in the 36-Well Rotor$209.00Add To CartCat No./ID:901890736-Well RotorFor holding PCR Tubes, 0.2 ml, with reaction volumes of 20–50 µl; requires 36-Well Rotor Locking Ring$553.00Add To CartCat No./ID:382615Investigator 26plex QS Kit (100)Primer Mix, Fast Reaction Mix 3.0includingTaqDNA Polymerase, Control DNA, allelic ladder 26plex, and nuclease-free water$2,344.00Add To CartCat No./ID:972807PyroMark Gold Q96 Reagents (50 x 96)For performing Pyrosequencing reactions on the PyroMark Q96 MD$1,653.00Add To CartCat No./ID:51161QIAamp 96 DNA Blood Kit (4)For 4 x 96 DNA preps: 4 QIAamp 96 Plates, QIAGEN Protease, Reagents, Buffers, Lysis Blocks, Tape Pads, Collection Vessels$1,305.00Add To CartCat No./ID:28183QIAquick 96 PCR Purification Kit (24)For purification of 24 x 96 PCR reactions: 24 QIAquick 96 Plates, Buffers, Collection Microtubes (1.2 ml), Caps$3,326.00Add To CartCat No./ID:77164exoRNeasy Maxi Kit (50)For purification of total exosome-derived RNA; includes 50 exoEasy Maxi columns and 50 RNeasy MinElute spin columns$2,305.00Add To CartCat No./ID:158912Protein Precipitation Solution (350 ml)350 ml Protein Precipitation Solution$417.00Add To CartCat No./ID:14700-50-NFRNeasy PowerWater Kit (50)For the isolation of total RNA from filtered water samples, including turbid water$560.00Add To CartCat No./ID:13000-V1-5Vortex Adapter for 6 (5 ml) tubesFor vortexing 5 and 15 ml tubes using the Vortex-Genie 2 Vortex.$126.00Add To CartCat No./ID:386015DNA Size Standard 550 (BTO) (100)DNA Size Standard 550 (BTO) for 100 reactions$134.00Add To CartCat No./ID:55284QIAamp MinElute ccfDNA Midi Kit (50)For 50 preps (4 or 5 ml sample input volume each): QIAamp UCP MinElute Columns, QIAGEN Proteinase K, Magnetic Bead Suspension, Buffers, Bead Elution Tubes, and Collection Tubes (1.5 ml and 2 ml)$977.00Add To CartCat No./ID:12243QIAfilter Plasmid Midi Kit (25)25 QIAGEN-tip 100, Reagents, Buffers, 25 QIAfilter Midi Cartridges$360.00Add To CartCat No./ID:239045 GelPilot 100 bp Plus Ladder (100) Ready-to-use DNA marker; 600 µl (100 lanes), 11 fragments: 100–1500 bp$176.00Add To CartCat No./ID:74524RNeasy Protect Bacteria Mini Kit (50)RNeasy Mini Kit (50) and RNAprotect Bacteria Reagent (2 x 100 ml)$528.00Add To CartCat No./ID:158924RNase A Solution (5 ml)5 ml RNase A Solution$446.00Add To Cart