Sample Collection & StabilizationDNA5RNA19Tissue/FFPE5PAXgene Blood RNA MDx KitPrint For fully automated, high-throughput purification of cellular RNA from blood stabilized in PAXgene Blood RNA TubesInstant stabilization of RNA at collectionOptimized purification of high-quality RNAFully automated RNA purification procedure The PAXgene Blood RNA MDx Kit is for fully automated, high-throughput purification of cellular RNA on the BioRobot MDx or BioRobot Universal System. (The PAXgene 96 Blood RNA Kit provides the same 96-well technology in a manual format.) Used with PAXgene Blood RNA Tubes (available from BD, cat. no. 762165) for sample collection and stabilization, the system provides a complete preanalytical solution for collection and stabilization through to purification of high-quality RNA to support high-throughput projects, such as clinical research studies. Buy ProductsProduct DetailsSpecificationsProduct ResourcesBuy ProductsCat No./ID:762431PAXgene Blood RNA MDx Kit (4)$4,288.00Order ProductFor 4 x 96 RNA preps on the BioRobot MDx workstation: 4 PAXgene 96 RNA Plates, 4 PAXgene 96 Filter Plates, Buffers (wash buffers are labeled with bar codes), Proteinase K, RNase-Free DNase Set, Plasticware, Collection Vessels. To be used with PAXgene Blood RNA TubesThis product will be discontinued as of December 31, 2020 or until stocks last. We recommend PAXgene 96 Blood RNA Kit (4).Cat No./ID:9016753Shaker-Adapter, 24-tube, PAXgene$678.00Order ProductAdapter for PAXgene Blood RNA Tubes on the shaker unit of the BioRobot Universal SystemCat No./ID:9238279PAXgene 96 Incubator Block$339.00Order ProductBlock for denaturation of eluates in PAXgene 96 proceduresFor Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.Product Details Very low levels of genomic DNA.RNA was purified from 384 blood samples collected in PAXgene Blood RNA Tubes and processed in 4 runs on the BioRobot MDx using the PAXgene Blood RNA MDx Kit. Genomic DNA was quantified using a genomic-DNA-specific real-time TaqMan PCR assay with primers and probe for the β-actin gene. The amount of DNA present is given as a percentage of the total nucleic acid content.High intersystem reproducibility.Blood was collected in fivefold replicates from 6 donors into PAXgene Blood RNA Tubes. RNA was purified using the PAXgene Blood RNA MDx Kit on 3 different BioRobot MDx workstations (MDx 1, MDx 2, MDx 3) and manually using the PAXgene 96 Blood RNA Kit (PAX 96). Purified RNA was analyzed using quantitative, real-time RT-PCR, with primers and probe for the c-fos transcript. All values were well within the ±3x total precision of the assay (2.34 CT), indicated by the horizontal lines.High quality RNA from different donors.Blood was collected in duplicate from 3 donors into PAXgene Blood RNA Tubes. RNA was purified using the PAXgene Blood RNA MDx Kit on the BioRobot MDx instrument. RNA was analyzed with an Agilent 2100 Bioanalyzer. The RNA integrity number (RIN), indicated on each scan, shows the high quality of the purified RNA. [A] Analysis of 2 samples from donor A. [B] Analysis of 2 samples from donor B. [C] Analysis of 2 samples from donor C.Automated PAXgene RNA purification procedure.Following collection of blood samples (2.5 ml) in PAXgene Blood RNA Tubes, the simple purification procedure begins with a centrifugation step to pellet the contents of each PAXgene Blood RNA Tube. Proteinase K and an optimized incubation buffer are added to digest proteins. The tubes are then transferred to the BioRobot MDx or BioRobot Universal System for automated RNA purification.No RT-PCR inhibition detected.A total of 48 water samples were processed on the BioRobot MDx workstation using the PAXgene Blood RNA MDx Kit. The eluates were pooled and added to quantitative, real-time RT-PCR assays at the percentage indicated. Each assay was performed in quadruplicate using the same amount of RNA from HeLa cells, with primers and probe for β-actin. [A] CT values are given (mean ± standard deviation), with the difference between the mean CT values and the mean CT value for the 0% reactions (ΔCT). None of the reactions were inhibited (ΔCT ≥0.5). [B] Amplification curves for all 20 assays show no detectable inhibition of RT-PCR.Performance The automated PAXgene Blood RNA procedure provides high-quality RNA with high intra-donor repeatability (see figure "High-quality RNA from different donors"). Since yields are highly donor-dependent, individual yields may vary. An integrated DNase treatment ensures that genomic DNA contamination is minimized, typically to <0.1% (see figure "Very low levels of genomic DNA"). The fully automated PAXgene Blood RNA procedure provides RNA yields comparable to the manual PAXgene 96 Blood RNA procedure (typically ≥80%). Typical yields of RNA isolated from 2.5 ml healthy, human, whole blood (4.6 x 106 – 1.1 x 107 leukocytes/ml) are ≥3 µg for >95% of the samples processed. Purified RNA is ready to use in downstream applications, such as quantitative, real-time RT-PCR, with no RT-PCR inhibition detected (see figure "No RT-PCR inhibition detected"). Intersystem variation is minimal and well within the error range of typical real-time RT-PCR assays (see figure "High intersystem reproducibility"). The robust and standardized procedure is designed to minimize the risk of sample-to-sample cross-contamination. Total RNA was purified from 48 blood samples collected in PAXgene Blood RNA Tubes and 48 water samples. The samples were arranged in a checkerboard pattern on a 96-well block. After purification using the PAXgene Blood RNA MDx Kit on the BioRobot MDx workstation, all samples were analyzed by quantitative, real-time PCR using primers and probes specific for 18s rRNA for 40 PCR cycles. Threshold cycle values (CT) for the blood samples ranged from 27.3 to 32.7 while no RNA was detected in the water samples after 40 cycles.  Principle Blood samples (2.5 ml) are collected directly into PAXgene Blood RNA Tubes, and RNA is isolated using the PAXgene Blood RNA MDx Kit. PAXgene Blood RNA Tubes contain a proprietary blend of reagents that bring about immediate stabilization of RNA. Blood samples can be stored in the PAXgene Blood RNA Tubes for up to 5 days at room temperature. RNA is then isolated by an automated procedure using silica membrane technology supplied in the PAXgene Blood RNA MDx Kit. The resulting RNA accurately represents the expression profile in vivo and is suitable for use in a range of downstream applications. ProcedureThe simple purification procedure begins with a centrifugation step to pellet the contents of each PAXgene Blood RNA Tube. Proteinase K and an optimized incubation buffer are added to digest proteins. The tubes are then transferred to the BioRobot MDx or BioRobot Universal System. After setup of buffers and plasticware, guided by the QIAsoft Operating System, the BioRobot workstation carries out the fully automated RNA purification procedure (see flowchart "Automated PAXgene RNA purification procedure"). Following purification, a final heat treatment of the eluate enhances performance in downstream applications. Total processing time is 3–4 hours, with a maximum of 25–30 minutes of hands-on time. Protocols are available for batches of 48 or 96 samples per run.Applications The purified RNA is ready for use in a wide range of downstream applications, including: High-throughput, quantitative, real-time RT-PCR Expression-array and expression-chip analysis cDNA synthesis RNase and S1 nuclease protection Northern, dot, and slot blot analysis Primer extension SpecificationsFeaturesSpecifications ApplicationsPCR, real-time PCR, qPCR, NASBA, cDNA synthesis Elution volume120 µl For automated processingBioRobot MDx workstation, BioRobot Universal System Format96-well plate Main sample typeWhole blood ProcessingAutomated Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinCellular RNA RUO/CE/ASRRUO Sample amount2.5 ml Samples per run (throughput)48 or 96 samples TechnologySilica technology Time per run or per prep3–4 hours Yield>3 µgProduct ResourcesYou are not authorized to download the resourceBrochures & Guides (2) Sort optionsSort alphabetically (EN) - Analyzing Gene Expression and RegulationShow detailsPAXgene Blood RNA MDx Kit Brochure (EN)For high-throughput purification of cellular RNA from whole bloodShow detailsShow allShow lessFAQs (16) Sort optionsSort alphabetically Can the PAXgene Blood RNA Tubes undergo freeze/thaw cycles?FAQ ID -2471ViewWhat is the PAXgene 96 filter plate?FAQ ID -2486ViewCan the PAXgene Blood RNA MDx Kit be used for diagnostic or prognostic procedures?FAQ ID -2488ViewCan the PAXgene Blood RNA MDx Kit be used with both the BioRobot Universal System and the BioRobot MDx?FAQ ID -2489ViewHow many samples can be processed with the PAXgene Blood RNA MDx Kit?FAQ ID -2490ViewWhat batch sizes can be selected with the PAXgene Blood RNA MDx Kit?FAQ ID -2491ViewIf less than 48/96 samples are being processed, is it possible to leave positions empty with the PAXgene Blood RNA MDx Kit?FAQ ID -2492ViewIs there a critical step during upfront sample handling when using the PAXgene Blood RNA MDX kit?FAQ ID -2493ViewWhy is a blood collection set required to be used with the PAXgene Blood RNA tube?FAQ ID - 3459ViewIs there any chance of the PAXgene Blood RNA tube reagent going back into the patient"s arm?FAQ ID - 3460ViewCan the PAXgene Blood RNA System be used with animal blood samples? FAQ ID - 3461ViewWhat are possible reasons for blood draws with lower than expected blood volume? FAQ ID - 3462ViewHow long can the PAXgene Blood RNA tubes be kept frozen at –20°C or –70°C? FAQ ID - 3463ViewWhat RNA yields are expected with the PAXgene Blood RNA kit?FAQ ID - 3464ViewWhat is the integrity of RNA from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?FAQ ID -3046ViewWhere can I find additional information for PreAnalytiX PAXgene products? FAQ ID - 3515ViewShow allShow lessKit Handbooks (1) Sort optionsSort alphabetically (EN) - PAXgene Blood RNA MDx Kit HandbookShow detailsShow allShow lessScientific Posters (2) Sort optionsSort alphabetically Automated, Magnetic Bead miRNA Enrichment from Stabilized Whole Blood: Development of an Optimized Protocol (EN)Voss et al., AMP 2010Show detailsHigh-Throughput Automation for RNA Isolation from Blood Stabilized in PAXgene Blood RNA TubesVoss et al., AMP 2005Show detailsShow allShow lessApplication Notes (1) Sort optionsSort alphabetically PAXgene Blood RNA MDx System Technical NoteTypical total RNA yields from PAXgene Blood RNA Tubes processed with BioRobot instruments and the PAXgene Blood RNA MDx KitShow detailsShow allShow lessSafety Data Sheets (2) Sort optionsSort alphabetically MSDS PAXgene Blood RNA MDx Kit (4)CofAShow allShow lessfragment fix placeholderCustomers who bought these products also boughtCat No./ID:762331PAXgene 96 Blood RNA Kit (4)4 PAXgene 96 RNA Plates, 4 PAXgene 96 Filter Plates, Buffers, Proteinase K, RNase-free DNase Sets, AirPore Tape Sheets, Collection Vessels. To be used in conjunction with PAXgene Blood RNA Tubes.$3,870.00Add To CartCat No./ID:204484QuantiFast Probe RT-PCR Plus Kit (400)For 400 x 25 µl reactions: 1.3 ml 2x QuantiFast Mix 1 (x2), 1.3 ml 2x QuantiFast Mix 2 (x 2), 100 µl RT Mix, 210µl ROX Dye Solution, 210 µl High-ROX Dye Solution, 1.9ml RNase-Free Water$712.00Add To CartCat No./ID:19504QIAvac 96Vacuum manifold for processing QIAGEN 96-well plates: includes QIAvac 96 Top Plate, Base, Waste Tray, Plate Holder, Rack of Collection Microtubes (1.2 ml)$748.00Add To CartCat No./ID:19585S-Blocks (24)96-well blocks with 2.2 ml wells, 24 per case$348.00Add To CartCat No./ID:79656QIAshredder (250)250 disposable cell-lysate homogenizers for use in nucleic acid minipreps, caps$406.00Add To Cart