Lab EssentialsBuffers & Reagents51Centrifuges3Enzymes5Gel Electrophoresis2Magnets4Plastics34Vacuum Manifolds & Accessories8QIAprep Spin Miniprep ColumnsPrint For purification of up to 20 μg molecular biology grade plasmid DNA Ready-to-use plasmid DNA in minutesReproducible yields of molecular biology grade plasmid DNASingle protocol for high- and low-copy vectors The QIAprep Spin Miniprep Kit is designed for isolation of up to 20 μg high-purity plasmid or cosmid DNA for use in routine molecular biology applications such as fluorescent and radioactive sequencing and cloning. Buy ProductsProduct DetailsSpecificationsProduct ResourcesBuy ProductsCat No./ID:27115QIAprep 2.0Spin Miniprep Columns (100 spin columns)$98.90Order ProductFor high-purity plasmid miniprepsThe QIAprep Spin Miniprep Columns is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.Product Details Spin column handling options — B.QIAvac 24 Plus.Spin column handling options — C.Spin column handling options — A.Complete digestion with various restriction enzymes.The QIAprep procedure.Performance The QIAprep Spin Miniprep Kit enables purification of up to 20 μg molecular biology grade plasmid DNA or cosmid DNA for use in routine molecular biology applications such as PCR, sequencing and cloning. Versatile QIAprep spin columns can be used either in microcentrifuges, on vacuum manifolds, or in the QIAcube (see figures "QIAprep Spin Column handling options A, B, and C"). The vacuum procedure provides simplified handling and faster sample processing. QIAprep spin columns can be vacuum processed using the QIAvac 24 Plus or any other commercial manifold with luer connectors. The QIAprep Spin Miniprep Kit can now also be fully automated on the QIAcube.   QIAprep Spin Miniprep Kit specificationsFormat:Spin columnsPurification module:QIAprep spin columns Throughput:1–24 samples Preparation time:24 minipreps in 30 minutes Equipment required:Microcentrifuge or vacuum manifold; fully automatable using the QIAcube Lysate clearing:CentrifugationCapacity of column reservoir:800 µlMinimum elution buffer volume:50 µlCulture volume for high-copy plasmids:1–5 mlCulture volume for low-copy plasmids/cosmids:1–10 mlPurified DNA can be used in restriction digestion (see figure "Complete digestion with various restriction enzymes").  PrincipleQIAprep spin columns contain a unique silica membrane that binds up to 20 μg DNA in the presence of a high concentration of chaotropic salt, and allows elution in a small volume of low-salt buffer. QIAprep membrane technology eliminates time consuming phenol–chloroform extraction and alcohol precipitation, as well as the problems and inconvenience associated with loose resins and slurries. High-purity plasmid DNA eluted from QIAprep spin columns is immediately ready to use — there is no need to precipitate, concentrate, or desalt.Procedure Plasmid purification using QIAprep Kits follows a simple bind-wash-elute procedure (see flowchart "The QIAprep procedure"). First, bacterial cultures are lysed and the lysates are cleared by centrifugation. The cleared lysates are then applied to the QIAprep module where plasmid DNA adsorbs to the silica membrane. Impurities are washed away and pure DNA is eluted in a small volume of elution buffer or water. In addition to plasmid purification from Escherichia coli, QIAprep Kits can be used to purify plasmid DNA from Saccharomyces cerevisiae, Bacillus subtilis, and Agrobacterium tumefaciens. Contact QIAGEN Technical Services or your local distributor for protocols for these applications.Applications The QIAprep Miniprep Kits provides reproducible yields of high-purity DNA suitable for use in most applications, including: PCR Restriction digestion Ligation and transformation Sequencing Screening SpecificationsFeaturesSpecifications ApplicationsFluorescent and radioactive sequencing (including capillary sequencing), ligation, cloning, transformation etc. Culture volume/starting material1–10 ml culture volume Elution volume50 µl (minimal) Plasmid typeHigh-copy, low-copy, cosmid DNA ProcessingManual (vacuum or centrifugation) Samples per run (throughput)1–24 samples per run TechnologySilica technology Time per run or prep per run YieldProduct ResourcesYou are not authorized to download the resourceApplication/Protocol Documents (1) Sort optionsSort alphabetically QIAprep Spin Miniprep Kit High-Yield Protocol - English (PDF)For purification of up to 30 μg plasmid DNAShow detailsShow allShow lessApplication Notes (1) Sort optionsSort alphabetically (EN) - QIAprep Spin Miniprep Kit – CompetitionShow detailsShow allShow lessUser-Developed Protocols (4) Sort optionsSort alphabetically Isolation of Plasmid DNA from Bacillus subtilis using the QIAprep® Spin Miniprep Kit - (EN)Show detailsIsolation of plasmid DNA from Agrobacterium using the QIAprep® Spin Miniprep Kit; spin procedure - (EN)Show detailsIsolation of plasmid DNA from Agrobacterium using the QIAprep® Spin Miniprep Kit; vacuum procedure - (EN)The procedure has been used successfully for isolation of a single-copy, 14.5 kb, binary plasmid, p35S GUS INT, from Agrobacterium tumefaciens strain GV2260 (1).Show detailsIsolation of plasmid DNA from yeast using the QIAprep® Spin Miniprep Kit - (EN)Show detailsShow allShow lessSupplementary Protocols (1) Sort optionsSort alphabetically QIAprep Spin Miniprep Kit High-Yield Protocol - (EN)Show detailsShow allShow lessfragment fix placeholderCustomers who bought these products also boughtCat No./ID:28115QIAquick Spin Columns (100)For DNA fragment purification from PCR, gel fragments and enzymatic reactions$125.00Add To CartCat No./ID:27106QIAprep Spin Miniprep Kit (250)For 250 high-purity plasmid minipreps: 250 QIAprep 2.0 Spin Columns, Reagents, Buffers, Collection Tubes (2 ml)$422.00Add To CartCat No./ID:19064Buffer N3 (500 ml)500 ml Neutralization Buffer$87.00Add To CartCat No./ID:19065Buffer PE (concentrate, 100 ml)100 ml Wash Buffer (5x concentrate for 500 ml buffer)$89.30Add To CartCat No./ID:19052Buffer P2 (500 ml)500 ml Lysis Buffer$75.30Add To CartCat No./ID:19066Buffer PB (500 ml)500 ml Binding Buffer$89.70Add To CartCat No./ID:19051Buffer P1 (500 ml)500 ml Resuspension Buffer (RNase A not included)$76.00Add To CartCat No./ID:27104QIAprep Spin Miniprep Kit (50)For 50 high-purity plasmid minipreps: 50 QIAprep 2.0 Spin Columns, Reagents, Buffers, Collection Tubes (2 ml)$96.90Add To CartCat No./ID:19063Buffer QG (250 ml)250 ml Solubilization and Binding Buffer (with pH indicator)$58.90Add To CartCat No./ID:28706QIAquick Gel Extraction Kit (250)For gel extraction or cleanup of 250 reactions: 250 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)$579.00Add To CartCat No./ID:28106QIAquick PCR Purification Kit (250)For purification of 250 PCR reactions: 250 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)$561.00Add To CartCat No./ID:28704QIAquick Gel Extraction Kit (50)For gel extraction or cleanup of 50 reactions: 50 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)$124.00Add To CartCat No./ID:28104QIAquick PCR Purification Kit (50)For purification of 50 PCR reactions: 50 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)$123.00Add To CartCat No./ID:12943QIAGEN Plasmid Plus Midi Kit (25)25 QIAGEN Plasmid Plus Midi Columns, Extender Tubes, Reagents, Buffers, 25 QIAfilter Midi Cartridges$359.00Add To CartCat No./ID:12143QIAGEN Plasmid Midi Kit (25)25 QIAGEN-tip 100, Reagents, Buffers$303.00Add To CartCat No./ID:12362EndoFree Plasmid Maxi Kit (10)10 QIAGEN-tip 500, Reagents, 10 QIAfilter Maxi Cartridges, Endotoxin-free Buffers$355.00Add To CartCat No./ID:19101RNase A (17,500 U)2.5 ml (100 mg/ml; 7000 units/ml, solution)Unit definition: That amount of enzyme causing the hydrolysis of RNA at a rate such that k (velocity constant) equals unity (Kunitz units) at 25°C and pH 5.0.$226.00Add To CartCat No./ID:69504 DNeasy Blood & Tissue Kit (50) 50 DNeasy Mini Spin Columns, Proteinase K, Buffers, Collection Tubes (2 ml)$171.00Add To CartCat No./ID:12163QIAGEN Plasmid Maxi Kit (25)25 QIAGEN-tip 500, Reagents, Buffers$591.00Add To CartCat No./ID:1031576Buffer TCL (125 ml)125 ml of lysis buffer for TurboCapture mRNA Kits$239.00Add To Cart