Protein PurificationCrystallization9Fractionation & Depletion10Tagged Protein Expression, Purification, Detection37Ni-NTA SuperflowPrint For purification of 6xHis-tagged proteins by FPLC&nbspBuy ProductsSpecificationsProduct ResourcesBuy ProductsCat No./ID:30410Ni-NTA Superflow (25 ml)$410.00Order Product25 ml nickel-charged resin (max. pressure: 140 psi)Cat No./ID:30430Ni-NTA Superflow (100 ml)$1,377.00Order Product100 ml nickel-charged resin (max. pressure: 140 psi)Cat No./ID:30450Ni-NTA Superflow (500 ml)$5,603.00Order Product500 ml nickel-charged resin (max. pressure: 140 psi)The Ni-NTA Superflow is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.SpecificationsFeaturesSpecifications ApplicationsProteomics Bead size60–160 µm Binding capacityUp to 50 mg/ml FPLCYes Gravity flow or spin columnGravity flow or automated Number of preps per run1–24 samples per run ProcessingManual ScaleLarge scale Special featureBatch and column purification Start materialCell lysate Support/matrixSuperflow Tag6xHis tag YieldDepends on binding capacityProduct ResourcesYou are not authorized to download the resourceFAQs (16) Sort optionsSort alphabetically What are the compatibilities of different reagents with Ni-NTA matrices?FAQ ID -49ViewHow can I remove imidazole from a protein sample?FAQ ID -91ViewHow can I eliminate contaminating protein in my Ni-NTA 6xHis-tag protein purification?FAQ ID -102ViewShould I use Ni-NTA Agarose in column or batch format for purification of 6xHis-tagged proteins?FAQ ID -147ViewWhat are the features and benefits of the QIAexpress 6xHis Tag System?FAQ ID -193ViewWhat are your recommendations for PCR template preparation for use with the EasyXpress Insect Kit II?FAQ ID -1221ViewHow can I check if any residual proteins remain on the Ni-NTA Agarose matrix after elution?FAQ ID -324ViewCan Ni-NTA resins be used to purify protein with an internal His-tag?FAQ ID -496ViewIs it possible to isolate both RNA and recombinant 6xHis-tagged protein from the same sample?FAQ ID -532ViewWhy do you recommend using Triton X for the purification of 6xHis-tagged protein? -100ViewWhat is the difference between Ni-NTA Agarose and Ni-NTA Superflow?FAQ ID -764ViewHow can I be sure that I am harvesting my induced bacterial culture at the best time point for protein expression?FAQ ID -788ViewAre the buffers in the Ni-NTA Fast Start Kit the same as the ones for use with Ni-NTA purchased separately?FAQ ID -791ViewCan I reuse the Ni-NTA Agarose and Ni-NTA Superflow resins?FAQ ID -802ViewWhich resin is used in the QIAexpress Ni-NTA Fast Start Columns?FAQ ID -836ViewDo you have a protocol for manual purification of 6xHis-tagged proteins expressed in E. coli using Ni-NTA Superflow?FAQ ID -967ViewShow allShow lessKit Handbooks (2) Sort optionsSort alphabetically The QIAexpressionist - (EN)A handbook for high-level expression and purification of 6xHis-tagged proteinsShow details(EN) - Important Note for Ni-NTA UsersShow detailsShow allShow lessTechnical Information (3) Sort optionsSort alphabetically Complete solutions for membrane protein analysis - (EN)Show detailsCritical factors for successful protein crystallization - (EN)Show detailsReliable purification of GST-, His-, and Strep-tagged proteins - (EN)Show detailsShow allShow lessScientific Posters (3) Sort optionsSort alphabetically (EN) - New Ni-NTA Cartridges — the faster way to purer proteinsShow details(EN) - Novel cell-free expression system for synthesis of proteins used in structural analysesShow details(EN) - Process Development: Scaling Up Human IL-1b Production, Tag Removal, and X-Ray CrystallographyShow detailsShow allShow lessQuick-Start Protocols (2) Sort optionsSort alphabetically Automated Purification of 6xHis-tagged Proteins from E. coli Using Ni-NTA Superflow under Native Conditions (EN)Show detailsNi-NTA Agarose Purification of 6xHis-tagged Proteins from E. coli under Native Conditions (EN)Show detailsShow allShow lessSafety Data Sheets (4) Sort optionsSort alphabetically MSDS Ni-NTA Superflow (100 ml)MSDS Ni-NTA Superflow (25 ml)MSDS Ni-NTA Superflow (500 ml)CofAShow allShow less References 0 fragment fix placeholderCustomers who bought these products also boughtCat No./ID:1007595Penta.His Antibody, (1000)Penta.His Antibody, (1000)$3,578.00Add To CartCat No./ID:150245REPLI-g FFPE Kit (100)DNA Polymerase, Buffers, and Reagents for 100 x 50 μl whole genome amplification reactions$1,876.00Add To CartCat No./ID:1048145QIAamp DNA Accessory Set AAdditional buffers and reagents for use with at least 12x QIAamp DNA Mini Kits (50), cat. no. 51304, or 8x QIAamp DNA Stool Mini Kits (50), cat. no. 51504, on the QIAcube$215.00Add To CartCat No./ID:30761Ni-NTA Superflow Cartridges (5 x 5 ml)5 cartridges pre-filled with 5 ml Ni-NTA Superflow: for automated purification of His-tagged proteins using liquid chromatography systems$644.00Add To CartCat No./ID:27361DirectPrep 96 MiniPrep Kit (4)For 4 x 96 plasmid minipreps: 4 DirectPrep 96 Plates, Buffers, Flat-Bottom Blocks and Lids, AirPore Tape Sheets, Tape Pads, Elution Microtubes RS, Caps$633.00Add To CartCat No./ID:69525 mericon DNA Bacteria Kit (100)Fast Lysis Buffer$123.00Add To CartCat No./ID:150363REPLI-g Advanced DNA Single Cell Kit (24)Buffers, enzymes and single cell storage solution for 24 WGA reactions$617.00Add To CartCat No./ID:30622Ni-NTA Superflow Columns (12 x 1.5 ml)For 12 6xHis-tagged protein preps: 12 polypropylene columns containing 1.5 ml Ni-NTA Superflow$428.00Add To CartCat No./ID:1014636Buffer G2 (260 ml)260 ml Lysis Buffer$85.50Add To CartCat No./ID:28306QIAquick Nucleotide Removal Kit (250)250 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)$573.00Add To CartCat No./ID:331595QIAseq miRNA NGS 48 Index IL (96)Sequencing adapters, primers and indexes compatible with Illumina platforms; two 48 indexes for 96 samples$1,113.00Add To CartCat No./ID:30230Ni-NTA Agarose (100 ml)100 ml nickel-charged resin (max. pressure: 2.8 psi)$1,114.00Add To CartCat No./ID:79237 Oligo-dT Primers (100 µl) 100 µl of oligo-dT primers (0.4 µg/ul) for cDNA synthesis with TurboCapture mRNA Kits$29.40Add To CartCat No./ID:218761miScript Plant RT Kit (50)For fifty 20 µl first strand cDNA synthesis reactions; 10x miScript Ligation Buffer, 10x miScript Plant Adaptor, 2x miScript Ligation Activator, miScript Plant RNA Ligase, 5x miScript RT Buffer, 10x miScript Plant RT Nucleics, miScript Plant Reverse Transcriptase, RNase-Free Water$520.00Add To CartCat No./ID:30004 Strep-Tactin Superflow Plus (10 ml)For purification of Strep-tagged proteins: 10 ml Strep-Tactin-charged Superflow Plus suspension (50%) containing0.02% sodium azide (max. pressure: 140 psi)$742.00Add To CartCat No./ID:150063REPLI-g WTA Single Cell Kit (24)REPLI-g SensiPhi DNA Polymerase, Buffers, and Reagents for 24 x 60 µl whole transcriptome amplification reactions (typical yield: 20 µg)$1,427.00Add To CartCat No./ID:79236 Random Hexamers (100 µl) 100 µl of random hexamers (0.4 µg/µl) for cDNA synthesis with TurboCapture mRNA Kits$28.50Add To CartCat No./ID:37901Qproteome Mammalian Protein Prep KitFor approximately 100 protein preparations from cultured mammalian cells: Buffer, Reagents, Protease Inhibitor Solution, Benzonase$234.00Add To CartCat No./ID:76214Buffer XE (45 ml)45 ml Elution Buffer for elution of intact extracellular vesicles$28.40Add To CartCat No./ID:301425Effectene Transfection Reagent (1 ml)1 ml Effectene Reagent, Enhancer, Buffer; for 40 transfections in 60 mm dishes or 160 transfections in 12-well plates$357.00Add To Cart