Protein PurificationCrystallization9Fractionation & Depletion10Tagged Protein Expression, Purification, Detection37Penta·His Antibody, BSA-free Print For sensitive detection of His-tagged proteins Detection of C-terminal, N-terminal, and internal His tagsHighly sensitive and specific detection of HHHHH epitopes Monoclonal mouse Anti·His Antibodies are used to detect recombinant proteins carrying His tags. Each of the three variants detects a different His tag epitope.  Like all QIAGEN mouse monoclonal antibodies, they are prepared under serum-free conditions, which guarantees the absence of viruses, mycoplasma, and contaminating immunoglobulins. Buy ProductsProduct DetailsSpecificationsProduct ResourcesBuy ProductsCat No./ID:34660Penta·His Antibody, BSA-free (100 μg)$842.00Order Product100 μg mouse anti-(H)5 (lyophilized, BSA-free, for 1000 ml working solution)The Penta·His Antibody, BSA-freeis intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.Product Details His-tagged thioredoxin detected by Penta·His Antibody in yeast cells.Identification of positive expression clones by colony blotting.Immunohistochemistry with Penta·His Antibodies.Sensitivity of QIAexpress Anti·His Antibodies.Performance Performance of Penta·His Antibody BSA-free PropertyValue Detection: N-terminal, C-terminal, and internal 6xHis tags Sensitivity in dot blots:* 0.5 ng Isotype: Mouse IgG1 Cross-reactivity: Negligible with crude E. coli, yeast, mammalian, or insect cell lysates Form: Lyophilized Working solution: 1000 ml * Detection using chromogenic substrate. Sensitivity may vary with different proteins. Many different proteins have been detected, via their His tags, using the QIAexpress Anti·His Antibodies (see table). For the sensitivity of the three Anti·His Antibodies in detecting this panel of proteins see the figure Sensitivity of QIAexpress Anti·His Antibodies)  Proteins tested with QIAexpress Anti·His Antibodies Protein Tag locationSequence A DHFR N-terminus MRGSHHHHHHGSVRPLNCIV... B DHFR C-terminus ...VYEKKDGSRSHHHHHH C Thioredoxin N-terminus MRGSHHHHHHGSSDKIIHLT... D TNFα N-terminus MRHHHHHHQSTESMIRDV... E TFIIAγ N-terminus MGSSHHHHHHSSGLVPRGSH... F Chaperonin C-terminus ...AKIAKDGSRSHHHHHH G DNA Polymerase C-terminus ...EDWLSAKERSHHHHHH H TFIIAαβ Internal ...QQQHQPQQQQHHHHHHQQAQPQQTVP...For other experiments with  Anti·His Antibodies see figures Identification of positive expression clones by colony blotting and His-tagged thioredoxin detected by Penta·His Antibodies in yeast cellsPrincipleAll QIAexpress mouse monoclonal antibodies are produced under conditions that guarantee the highest purity and activity. Hybridoma cell culture is carried out under serum-free conditions, ensuring that the preparations are free of viruses, mycoplasma, and contaminating immunoglobulins. Purification by adsorption chromatography is performed entirely at physiological pH, yielding preparations with the highest proportion of native antibody. See figure Immunohistochemistry with Penta·His Antibodies.Applications QIAexpress Detection Systems allow efficient detection of His-tagged proteins and are ideal for many applications, including: Colony, dot, and western blotting procedures  Screening for positive expression clones  Monitoring expression levels and stability of His-tagged proteins  Immunoprecipitation and ELISA SpecificationsFeaturesSpecifications DetectionSecondary antibody required Epitope detectedHHHHH Sensitivity in dot blots (chemiluminescent detection)10 pg Sensitivity in dot blots (chromogenic detection)0.5 ng Sensitivity in Western blots (chemiluminescent detection)50 pg Sensitivity in Western blots (chromogenic detection)2 ng Substrate for blot detectionDependent on secondary antibody Substrates for assay procedureDependent on secondary antibody TagHis-tagProduct ResourcesYou are not authorized to download the resourceFAQs (9) Sort optionsSort alphabetically What epitopes do the Anti-His Conjugates and Anti-His Antibodies recognize?FAQ ID -170ViewWhich Anti-His Antibody is the most sensitive for my protein of interest?FAQ ID -172ViewHow should I store Anti·His and Tag·100 Antibodies?FAQ ID -194ViewWhat blocking reagents do you recommend for chemiluminescent detection using Penta-, Tetra-, and RGS-His antibodies?FAQ ID -1093ViewAre QIAGEN monoclonal Anti His Antibodies glycosylated?FAQ ID -334ViewWhat are the dissociation constants for the Anti-His Antibodies?FAQ ID -385ViewWhat is the difference between BSA-free and BSA-containing RGS-His Antibody?FAQ ID -569ViewHow should I dissolve the lyophilized Anti-His and Tag Antibodies?-100ViewHow is an antibody epitope determined?FAQ ID -593ViewShow allShow lessKit Handbooks (1) Sort optionsSort alphabetically QIAexpress Detection and Assay Handbook - (EN)For Anti·His Antibodies,Anti·His HRP Conjugates, Penta·His™ Alexa Fluor® Conjugates, Penta·His Biotin Conjugate, Ni-NTA Conjugates, Tag·100™ Antibody, Streptavidin–R-PE, 6xHis Protein Ladder, Ni-NTA HisSorb™ Strips and PlatesShow detailsShow allShow lessSafety Data Sheets (2) Sort optionsSort alphabetically MSDS Penta·His Antibody, BSA-free (100 μg)CofAShow allShow less References 0 fragment fix placeholderCustomers who bought these products also boughtCat No./ID:34650RGS·His Antibody, BSA-free (100 μg)100 μg mouse anti·RGS(H)4 (lyophilized, BSA-free, for 1000ml working solution)$742.00Add To CartCat No./ID:34670Tetra·His Antibody, BSA-free (100 µg)100 µg mouse anti-(H)4 (lyophilized, BSA-free, for 1000 ml working solution)$842.00Add To CartCat No./ID:9018956 Reagent Block, 24x2ml Flat-Base Tubes Reagent Block; 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